##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR522124_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 27703451 Sequences flagged as poor quality 0 Sequence length 50 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 39.22974993259865 40.0 39.0 40.0 38.0 40.0 2 39.155648695175195 40.0 39.0 40.0 38.0 40.0 3 39.132366108467856 40.0 39.0 40.0 38.0 40.0 4 38.93017552217592 40.0 39.0 40.0 38.0 40.0 5 39.13636940033211 40.0 39.0 40.0 38.0 40.0 6 39.19773976895514 40.0 39.0 40.0 38.0 40.0 7 39.17473342942004 40.0 39.0 40.0 38.0 40.0 8 39.15904711654876 40.0 39.0 40.0 38.0 40.0 9 39.14503438578826 40.0 39.0 40.0 38.0 40.0 10 39.13069129907317 40.0 39.0 40.0 38.0 40.0 11 39.1582839986253 40.0 39.0 40.0 38.0 40.0 12 39.12554869066673 40.0 39.0 40.0 38.0 40.0 13 39.10703215278126 40.0 39.0 40.0 38.0 40.0 14 39.08746953583508 40.0 39.0 40.0 38.0 40.0 15 39.06366412617692 40.0 39.0 40.0 38.0 40.0 16 39.06032382030672 40.0 39.0 40.0 38.0 40.0 17 39.025881324315876 40.0 39.0 40.0 38.0 40.0 18 38.99831169048217 40.0 39.0 40.0 38.0 40.0 19 38.97141161222116 40.0 39.0 40.0 38.0 40.0 20 38.93446538483599 40.0 39.0 40.0 37.0 40.0 21 38.8983939582112 40.0 39.0 40.0 37.0 40.0 22 38.83192530778927 40.0 39.0 40.0 37.0 40.0 23 38.7567341339532 40.0 39.0 40.0 36.0 40.0 24 38.69383088771143 40.0 39.0 40.0 36.0 40.0 25 38.67631989242062 40.0 39.0 40.0 36.0 40.0 26 38.551492158864974 40.0 39.0 40.0 36.0 40.0 27 38.47334994474154 40.0 38.0 40.0 35.0 40.0 28 38.4234312902028 40.0 38.0 40.0 35.0 40.0 29 38.40426411135566 40.0 38.0 40.0 35.0 40.0 30 38.339765973560475 40.0 38.0 40.0 35.0 40.0 31 38.283270015710315 40.0 38.0 40.0 35.0 40.0 32 38.21731758256399 40.0 38.0 40.0 35.0 40.0 33 38.1822115591303 40.0 38.0 40.0 35.0 40.0 34 38.12864668015548 40.0 38.0 40.0 35.0 40.0 35 38.04665101109605 40.0 38.0 40.0 35.0 40.0 36 38.05410820478647 40.0 38.0 40.0 35.0 40.0 37 38.302558082023786 40.0 38.0 40.0 35.0 40.0 38 38.23160139868495 40.0 38.0 40.0 35.0 40.0 39 38.19975049317863 40.0 38.0 40.0 35.0 40.0 40 38.146607402810574 40.0 38.0 40.0 35.0 40.0 41 38.22163967947531 40.0 38.0 40.0 35.0 40.0 42 38.156031499469144 40.0 38.0 40.0 35.0 40.0 43 38.13178054965066 40.0 38.0 40.0 35.0 40.0 44 38.071213943706866 40.0 38.0 40.0 35.0 40.0 45 38.00404050744436 40.0 38.0 40.0 35.0 40.0 46 37.89626635324242 40.0 38.0 40.0 35.0 40.0 47 37.74737439750737 40.0 38.0 40.0 34.0 40.0 48 37.61750119145806 40.0 38.0 40.0 33.0 40.0 49 37.55267240893562 40.0 38.0 40.0 33.0 40.0 50 37.529461654434314 40.0 38.0 40.0 33.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1 1 -0.0708689152406663 1 2 -0.12203979897569894 1 3 -0.1351154006618529 1 4 0.04609367560109945 1 5 -0.09140453379871616 1 6 -0.16026644646520793 1 7 -0.10528294297575513 1 8 -0.13603306465519438 1 9 -0.11217681898907017 1 10 -0.1435532556357586 1 11 -0.12650589038296545 1 12 -0.14907789426820273 1 13 -0.1525394965857103 1 14 -0.16630057565750178 1 15 -0.13183780124162325 1 16 -0.14552670585085536 1 17 -0.1488880426592445 1 18 -0.17134434908032148 1 19 -0.1393608991250801 1 20 -0.13277416583769508 1 21 -0.14535174136324258 1 22 -0.20113038020458873 1 23 -0.23424319336491806 1 24 -0.25413834249467016 1 25 -0.2726793335220705 1 26 -0.0786033350173696 1 27 -0.056970330008653036 1 28 -0.031935280578650804 1 29 -0.27104560359658336 1 30 -0.19597561011843112 1 31 -0.19579043991978295 1 32 -0.100323530790007 1 33 -0.1389039612509535 1 34 -0.09169402055213993 1 35 -0.17814247710454367 1 36 -0.09612564114322453 1 37 -0.0938726421492646 1 38 -0.07153153517836586 1 39 -0.06869228621098245 1 40 -0.06107223460421807 1 41 -0.11038592890896126 1 42 -0.1074937808103158 1 43 -0.22125559320794963 1 44 -0.059938464544970316 1 45 -0.17608499703437275 1 46 -0.17409637502901631 1 47 0.026070129008438414 1 48 -0.23420962988222982 1 49 -0.2872047097142101 1 50 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120 20 -0.04978273729555127 120 21 -0.03395169032871337 120 22 -0.045862186719595854 120 23 -0.05502758042883471 120 24 0.003572523334185007 120 25 0.0049446659712870655 120 26 0.032037530254143576 120 27 0.003538814542793034 120 28 0.03542890440579072 120 29 0.04731449903768947 120 30 -0.0364108692224292 120 31 0.03063416029346655 120 32 0.03937629639824536 120 33 4.6382307680659096E-4 120 34 0.04931022198835677 120 35 0.030201401059471777 120 36 -0.009904298201860229 120 37 0.019233697387946336 120 38 0.02634230992774178 120 39 0.026086158623783717 120 40 0.02743451004117503 120 41 0.026917678270926615 120 42 0.02719259483900771 120 43 0.10692643739370311 120 44 0.10643306739493852 120 45 0.09511946931456094 120 46 0.10893731076144064 120 47 0.20351031270919862 120 48 0.16785922167923673 120 49 0.21659367039293187 120 50 0.12228703893394055 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5903.0 3 562.0 4 259.0 5 294.0 6 566.0 7 977.0 8 1272.0 9 1576.0 10 2336.0 11 3389.0 12 3966.0 13 5626.0 14 7816.0 15 9072.0 16 9774.0 17 11691.0 18 13936.0 19 16334.0 20 18641.0 21 19214.0 22 24610.0 23 31158.0 24 34880.0 25 42634.0 26 57123.0 27 74519.0 28 93158.0 29 114587.0 30 140605.0 31 175326.0 32 221653.0 33 291410.0 34 411476.0 35 620350.0 36 1006538.0 37 1870006.0 38 3670237.0 39 1.8202965E7 40 487012.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 17.6832177268599 29.439370783772322 24.95621238829972 27.921199101068055 2 23.779947536424718 29.430990039717674 26.17678282844873 20.61227959540888 3 23.266148481364944 28.04515204976982 25.69384346739765 22.99485600146759 4 23.12834232323578 27.048721992232295 26.53946030539523 23.283475379136696 5 23.90502947704971 27.882134539576832 27.239697543447438 20.97313843992602 6 23.77672009165934 26.997773670868657 25.336724294745807 23.888781942726197 7 23.52337259354439 27.385678412411508 26.78411075934186 22.30683823470224 8 23.172278949515622 27.040513074598927 26.930464042551762 22.856743933333686 9 22.500355470137638 26.44878672510082 27.421325587579776 23.62953221718177 10 22.706406097756908 27.737861946568138 26.29482418298122 23.26090777269373 11 23.20520071303801 27.30766715113131 26.434580379788326 23.052551756042355 12 23.026237894673006 27.05687319782779 26.75795987969395 23.158929027805254 13 22.76987966371985 27.59375917417358 26.52098300106965 23.11537816103692 14 22.81439399189818 27.801631655791464 26.718396072221047 22.665578280089314 15 22.572649613067366 27.187088896372476 27.090663024970407 23.149598465589747 16 23.177723251631345 27.137266014097584 26.730448420902547 22.95456231336852 17 23.025352959878763 27.0151041213955 26.81188742246809 23.147655496257645 18 22.610069774152482 27.005861594804564 26.733927287289326 23.650141343753624 19 23.351849441401622 27.10976822161402 26.6845539720943 22.85382836489006 20 23.008519768890885 27.320506026487458 26.874471342938467 22.796502861683187 21 23.156424198865476 27.03647429085589 26.756191317963467 23.05091019231517 22 22.913293365508867 27.237834015697178 26.69612894075904 23.152743678034913 23 22.77570133670313 26.950736415450184 27.435254841069234 22.838307406777453 24 22.78898838700893 26.85458747962477 26.825244480175353 23.531179653190943 25 23.34726096037638 26.77838584081095 26.659624463392667 23.21472873542 26 23.360444883298808 26.882039399793058 26.953893279337642 22.80362243757049 27 23.006815590652337 27.069317677490428 26.87324784561603 23.050618886241196 28 23.046320113692694 27.195229215306068 26.73155052054706 23.02690015045418 29 23.409404539437897 26.871199703430225 26.850429712133117 22.86896604499876 30 22.96152667423997 27.12138260096446 26.811671263713077 23.10541946108249 31 23.41730665686446 26.770443578547688 26.74161752776972 23.07063223681814 32 22.98929870056346 26.844375487583417 26.81197794251795 23.354347869335175 33 23.352434940468097 26.715128387651987 26.743301735910023 23.189134935969893 34 23.45514194338192 26.823897269758216 26.675416661747995 23.04554412511186 35 23.048238851154384 26.88197059486994 27.153422224314316 22.91636832966136 36 22.96065563887953 26.737495553190342 27.153776498789185 23.148072309140943 37 23.079176098417463 26.790753005571354 26.632185163980076 23.497885732031104 38 23.01599952698595 27.344200816700052 26.753691289170483 22.886108367143514 39 23.35793662161366 26.72146612476768 26.77687875193047 23.143718501688188 40 23.050986559503862 26.774701095298063 26.65032532047626 23.523987024721816 41 23.036042488234383 27.271383820324928 26.770690529645346 22.921883161795343 42 23.43584946960671 26.66640941614865 26.74734586771442 23.150395246530223 43 23.44862174893682 26.765325174090986 26.669313561732295 23.116739515239903 44 23.06151595631877 27.2316601375845 26.79219470111062 22.914629204986106 45 22.99080917168985 27.09277810479364 26.732280564042387 23.18413215947412 46 23.061524820909405 26.77070357732147 27.052592788396616 23.115178813372506 47 23.52429538771503 26.78270092721742 26.753029507865577 22.939974177201975 48 23.02561693898234 26.599302583496286 26.737336242050976 23.637744235470407 49 23.089856763667598 26.748517133933447 26.609025462030967 23.552600640367988 50 23.54691771847131 26.768071391082177 26.693033074181375 22.991977816265134 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 6754.0 1 3893.5 2 1033.0 3 862.0 4 691.0 5 782.5 6 874.0 7 1056.0 8 1238.0 9 2162.5 10 3087.0 11 5595.0 12 8103.0 13 13580.0 14 19057.0 15 28535.0 16 38013.0 17 57022.5 18 76032.0 19 103964.0 20 131896.0 21 174055.0 22 216214.0 23 288187.5 24 360161.0 25 400262.0 26 440363.0 27 518508.0 28 596653.0 29 681348.0 30 766043.0 31 853057.5 32 940072.0 33 1030878.5 34 1121685.0 35 1226361.0 36 1331037.0 37 1421054.0 38 1511071.0 39 1580633.5 40 1650196.0 41 1701438.0 42 1752680.0 43 1770781.0 44 1788882.0 45 1789047.5 46 1789213.0 47 1778982.5 48 1768752.0 49 1743160.5 50 1717569.0 51 1674719.0 52 1631869.0 53 1586915.0 54 1541961.0 55 1467980.5 56 1394000.0 57 1325166.0 58 1256332.0 59 1225567.0 60 1194802.0 61 1029573.5 62 864345.0 63 743792.5 64 623240.0 65 528122.0 66 433004.0 67 360313.5 68 287623.0 69 236970.5 70 186318.0 71 152786.5 72 119255.0 73 92204.0 74 65153.0 75 48429.0 76 31705.0 77 24330.5 78 16956.0 79 12947.5 80 8939.0 81 6783.0 82 4627.0 83 3555.5 84 2484.0 85 1917.0 86 1350.0 87 1189.5 88 1029.0 89 714.5 90 400.0 91 273.0 92 146.0 93 104.5 94 63.0 95 55.0 96 47.0 97 65.5 98 84.0 99 217.0 100 350.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1024637688640307 2 0.0015196662682927119 3 0.0031656705873936066 4 0.044698402375935045 5 1.227283922136632E-4 6 0.0 7 0.0 8 0.0012453322151092295 9 0.002533980333352693 10 6.49738547013511E-5 11 6.858351329587061E-5 12 0.0027830467763745393 13 7.219317189039012E-6 14 2.1296985707665083E-4 15 0.004844161833845177 16 4.5481698290945774E-4 17 6.858351329587061E-5 18 2.490664430218459E-4 19 1.8048292972597528E-5 20 0.0 21 7.219317189039011E-5 22 0.0 23 2.418471258328069E-4 24 2.8877268756156044E-4 25 0.0 26 2.1657951567117036E-5 27 2.021408812930923E-4 28 0.0 29 7.580283048490963E-5 30 0.003180109221771685 31 0.022675875290771537 32 0.024935521570940745 33 0.012680730642547025 34 0.010338062214703864 35 0.004613143683795929 36 0.003631316546086623 37 0.0017398554425584018 38 9.27682258791513E-4 39 0.0013680606073228929 40 0.0011081651885174884 41 2.3101815004924838E-4 42 8.482697697120839E-4 43 0.0019925315441747674 44 0.001718197490991285 45 2.598954188054044E-4 46 5.053522032327308E-5 47 8.663180626846815E-5 48 2.2740849145472887E-4 49 1.8048292972597528E-5 50 9.782174791147862E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 2.7703451E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.75028837738443 #Duplication Level Percentage of deduplicated Percentage of total 1 70.75122271738064 30.246351742205746 2 14.595044025452959 12.478846819374711 3 5.067715306275301 6.499388722732624 4 2.5789567344651654 4.410045764447338 5 1.5044241770123863 3.2157283704594373 6 1.0064984963283108 2.5816860581663454 7 0.7056299913784846 2.1116119933413056 8 0.5468982040355749 1.8704044748475561 9 0.39064740472660253 1.5030260285345158 >10 2.522545009867725 20.951171652002785 >50 0.23351326485406698 6.79710331819953 >100 0.09671294078696091 6.651926654117115 >500 1.1552708602312593E-4 0.02835862249560933 >1k 2.5500085324036448E-5 0.037048919106132096 >5k 3.3800176248190875E-5 0.09493073799539266 >10k+ 1.690008812164731E-5 0.5223701219738417 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCG 109916 0.396759234075206 Illumina Paired End PCR Primer 2 (100% over 31bp) AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 34688 0.12521183732669264 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 1.4438634378078024E-5 3.609658594519506E-6 0.0 0.0 0.0 2 2.1657951567117036E-5 7.219317189039012E-6 3.609658594519506E-6 0.0 0.0 3 2.5267610161636542E-5 7.219317189039012E-6 3.609658594519506E-6 0.0 0.0 4 3.609658594519506E-5 1.0828975783558518E-5 3.609658594519506E-6 0.0 0.0 5 4.331590313423407E-5 1.4438634378078024E-5 3.609658594519506E-6 0.0 0.0 6 4.331590313423407E-5 3.248692735067555E-5 3.609658594519506E-6 0.0 0.0 7 5.0535220323273084E-5 3.248692735067555E-5 3.609658594519506E-6 0.0 0.0 8 6.49738547013511E-5 3.248692735067555E-5 3.609658594519506E-6 3.609658594519506E-6 0.0 9 9.024146486298764E-5 3.248692735067555E-5 3.609658594519506E-6 7.219317189039012E-6 0.0 10 4.4398800712589924E-4 3.248692735067555E-5 3.609658594519506E-6 7.219317189039012E-6 0.0 11 4.945232274491723E-4 3.9706244539714564E-5 3.609658594519506E-6 7.219317189039012E-6 0.0 12 6.316902540409135E-4 3.9706244539714564E-5 3.609658594519506E-6 1.0828975783558518E-5 0.0 13 7.327606946874597E-4 3.9706244539714564E-5 3.609658594519506E-6 1.0828975783558518E-5 0.0 14 7.977345493888108E-4 3.9706244539714564E-5 3.609658594519506E-6 1.0828975783558518E-5 0.0 15 0.004443489729853512 4.331590313423407E-5 3.609658594519506E-6 1.0828975783558518E-5 0.0 16 0.004703385148658916 5.0535220323273084E-5 3.609658594519506E-6 1.0828975783558518E-5 0.0 17 0.0053711719886450245 5.7754537512312095E-5 3.609658594519506E-6 1.4438634378078024E-5 0.0 18 0.006114761659116043 5.7754537512312095E-5 3.609658594519506E-6 1.804829297259753E-5 0.0 19 0.008074806275940134 5.7754537512312095E-5 3.609658594519506E-6 1.804829297259753E-5 0.0 20 0.4267554969956631 6.13641961068316E-5 3.609658594519506E-6 1.804829297259753E-5 0.0 21 0.4327836268485107 6.13641961068316E-5 3.609658594519506E-6 1.804829297259753E-5 0.0 22 0.4347905970270635 7.580283048490962E-5 3.609658594519506E-6 1.804829297259753E-5 0.0 23 0.4356569150897482 7.580283048490962E-5 3.609658594519506E-6 1.804829297259753E-5 0.0 24 0.43603231958357824 7.941248907942913E-5 3.609658594519506E-6 1.804829297259753E-5 0.0 25 0.4363716274914631 7.941248907942913E-5 3.609658594519506E-6 1.804829297259753E-5 0.0 26 0.43690224730485744 8.302214767394864E-5 3.609658594519506E-6 1.804829297259753E-5 0.0 27 0.43735345462917236 8.302214767394864E-5 3.609658594519506E-6 1.804829297259753E-5 0.0 28 0.4563583071293176 8.302214767394864E-5 3.609658594519506E-6 1.804829297259753E-5 0.0 29 0.45720657689902966 8.302214767394864E-5 3.609658594519506E-6 1.804829297259753E-5 0.0 30 0.4587009755571607 8.302214767394864E-5 3.609658594519506E-6 1.804829297259753E-5 0.0 31 0.4830445131186003 8.663180626846815E-5 3.609658594519506E-6 2.1657951567117036E-5 0.0 32 0.48383141869220553 8.663180626846815E-5 3.609658594519506E-6 2.1657951567117036E-5 0.0 33 0.4843223322610602 8.663180626846815E-5 3.609658594519506E-6 2.5267610161636542E-5 0.0 34 0.48469412709629567 8.663180626846815E-5 3.609658594519506E-6 2.5267610161636542E-5 0.0 35 0.4850695315901257 8.663180626846815E-5 3.609658594519506E-6 2.5267610161636542E-5 0.0 36 0.48544132642536125 9.024146486298764E-5 3.609658594519506E-6 3.609658594519506E-5 0.0 37 0.4858022922848132 9.024146486298764E-5 3.609658594519506E-6 3.609658594519506E-5 0.0 38 0.4861704774614542 9.385112345750715E-5 3.609658594519506E-6 3.609658594519506E-5 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GATCGGA 15485 0.0 33.155064 21 AGATCGG 15970 0.0 32.2859 20 ATGCCGA 16190 0.0 32.06642 14 ATCGGAA 16710 0.0 30.750816 22 TGCCGAG 17265 0.0 30.171745 15 CGAGATC 17360 0.0 29.815052 18 AAGAGCG 17250 0.0 29.566118 27 AGAGCGG 17460 0.0 29.386936 28 GAGATCG 17620 0.0 29.374893 19 CCGAGAT 17705 0.0 29.320889 17 TCGGAAG 17610 0.0 29.279152 23 GAGCGGT 17685 0.0 28.714468 29 GCCGAGA 18270 0.0 28.53464 16 AGCGGTT 18150 0.0 27.978807 30 GCGGTTC 18245 0.0 27.965776 31 CGGAAGA 21270 0.0 24.355278 24 AATGCCG 27445 0.0 18.908194 13 GAAGAGC 28425 0.0 18.499819 26 CGGTTCA 28765 0.0 18.127838 1 GAATGCC 34120 0.0 15.467546 12 >>END_MODULE