FastQCFastQC Report
Thu 26 May 2016
SRR522122_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR522122_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32539399
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1057300.3249291727852749No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1037500.3188442417144828No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT582300.1789522910364755No Hit
TCTCTGAGCGGGCTGGCAAGGCAGACCGATATCGTATGCCGTCTTCTGCT361440.11107765081954957Illumina PCR Primer Index 1 (95% over 24bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT343530.1055735540782422No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT339940.10447027617197233No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA461700.034.688231
TATGCCG76200.032.2848135
TCGTATG79150.030.91519232
GACCGAT79600.030.82117724
CGTATGC82200.029.92871733
ATATCGT58950.029.14937429
TATCGTA59300.029.01675230
ACCGATA61000.028.49635325
CGATATC61750.027.90158527
AGACCGA91550.027.56688923
GATATCG63850.027.05200628
GCGGGCT95800.026.720178
CCGATAT66600.026.03458826
TGAGCGG101250.025.760175
ATCGTAT67950.025.55086731
GCCGTCT97250.025.2940838
CCGTCTT98150.025.0172739
ATGCCGT102250.024.01649536
CAGACCG106550.023.87032722
CGGGCTG111600.023.331289
Produced by FastQC (version 0.11.5)