FastQCFastQC Report
Thu 26 May 2016
SRR522121_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR522121_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33827012
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1529830.45225099988139655No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1467300.43376577274989586No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT808940.23914024685360918No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT523960.15489396462211916No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT512010.15136128488085204No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT459150.1357347199332888No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA587700.035.958771
TATGCCG42600.029.3911935
TCGTATG45150.027.211532
GACCGAT47700.026.06095924
CGTATGC48400.025.84746233
TATCGTA36150.024.31040830
ATATCGT36750.023.91302729
GTATCAA898350.023.587442
ACCGATA39800.022.030625
AGACCGA59950.021.59234823
CGATATC40650.021.51730527
GATATCG41450.021.31107328
GCGGGCT64200.020.9393948
ATCGTAT43950.020.14972931
CCGATAT45250.019.8678426
CCGTCTT63300.019.85990339
GTACATG2141100.019.7994381
TACATGG2145000.019.6022052
GCCGTCT65500.019.32712638
ACATGGG2220200.018.6511843
Produced by FastQC (version 0.11.5)