##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR522121_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	33827012
Sequences flagged as poor quality	0
Sequence length	50
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.79126403478971	40.0	39.0	40.0	37.0	40.0
2	38.63312124641692	40.0	39.0	40.0	36.0	40.0
3	38.58810272689767	40.0	39.0	40.0	36.0	40.0
4	38.63156985311029	40.0	39.0	40.0	36.0	40.0
5	38.4962751070062	40.0	39.0	40.0	36.0	40.0
6	38.748857954110754	40.0	39.0	40.0	36.0	40.0
7	38.69811291638765	40.0	39.0	40.0	36.0	40.0
8	38.679061041513215	40.0	39.0	40.0	36.0	40.0
9	38.72535803635272	40.0	39.0	40.0	36.0	40.0
10	38.640047604559335	40.0	39.0	40.0	36.0	40.0
11	38.785529120928565	40.0	39.0	40.0	36.0	40.0
12	38.71105825131703	40.0	39.0	40.0	36.0	40.0
13	38.719914014279475	40.0	39.0	40.0	36.0	40.0
14	38.68569801553859	40.0	39.0	40.0	36.0	40.0
15	38.66259603419894	40.0	39.0	40.0	36.0	40.0
16	38.63843803289513	40.0	39.0	40.0	36.0	40.0
17	38.5977133008378	40.0	39.0	40.0	36.0	40.0
18	38.57215287001997	40.0	39.0	40.0	36.0	40.0
19	38.5465952771708	40.0	39.0	40.0	36.0	40.0
20	38.420178997778464	40.0	38.0	40.0	35.0	40.0
21	38.39439714628061	40.0	38.0	40.0	35.0	40.0
22	38.33533343116442	40.0	38.0	40.0	35.0	40.0
23	38.233976001191	40.0	38.0	40.0	35.0	40.0
24	37.98405694242223	40.0	38.0	40.0	35.0	40.0
25	38.079191061865	40.0	38.0	40.0	35.0	40.0
26	37.81790389881317	40.0	38.0	40.0	35.0	40.0
27	37.50674797998712	40.0	38.0	40.0	33.0	40.0
28	37.69437557771878	40.0	38.0	40.0	33.0	40.0
29	37.60420568627226	40.0	38.0	40.0	33.0	40.0
30	37.28932818541585	40.0	38.0	40.0	33.0	40.0
31	37.07141340181036	40.0	38.0	40.0	33.0	40.0
32	36.99144937779311	39.0	38.0	40.0	33.0	40.0
33	36.76507165338754	39.0	38.0	40.0	32.0	40.0
34	36.5719579074853	39.0	38.0	40.0	31.0	40.0
35	36.72074057265241	39.0	37.0	40.0	31.0	40.0
36	36.44975630717842	39.0	37.0	40.0	31.0	40.0
37	36.72814045769103	39.0	38.0	40.0	32.0	40.0
38	36.821520298629984	40.0	38.0	40.0	32.0	40.0
39	36.76662050434724	40.0	38.0	40.0	32.0	40.0
40	36.67202982634115	40.0	38.0	40.0	31.0	40.0
41	36.83489378251913	40.0	38.0	40.0	33.0	40.0
42	36.77298580199753	40.0	38.0	40.0	33.0	40.0
43	36.70158617616005	40.0	38.0	40.0	32.0	40.0
44	36.59246560115922	40.0	38.0	40.0	32.0	40.0
45	36.43779760979184	40.0	38.0	40.0	31.0	40.0
46	36.02447635635095	40.0	38.0	40.0	31.0	40.0
47	36.11821203717313	39.0	37.0	40.0	31.0	40.0
48	36.03740525471183	39.0	37.0	40.0	31.0	40.0
49	35.95494461645031	39.0	37.0	40.0	30.0	40.0
50	35.88196492199784	39.0	37.0	40.0	30.0	40.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
3	1	-0.17766122176590926
3	2	-0.17674611388635242
3	3	-0.1875054757577317
3	4	-0.1542968594806311
3	5	0.0477709748668147
3	6	-0.2252478130673481
3	7	-0.19035743760328927
3	8	-0.2126644132034201
3	9	-0.21279342304797666
3	10	-0.2381261265071899
3	11	-0.2701078198847213
3	12	-0.27168937214533173
3	13	-0.28367868426501985
3	14	-0.376393581019542
3	15	-0.3226142971253836
3	16	-1.203237666978417
3	17	-1.2188051271141305
3	18	-1.8973351512880043
3	19	-1.6815381680695083
3	20	-10.92936762362174
3	21	-5.026748940871066
3	22	-3.332788836866392
3	23	-2.915580215735176
3	24	-2.761411127765868
3	25	-3.1385398419803963
3	26	-1.6820278388882102
3	27	-3.158580867396722
3	28	-4.980334642071661
3	29	-4.623076097683992
3	30	-2.69583965173166
3	31	-3.2594876834966584
3	32	-4.515261847205565
3	33	-2.526676784341504
3	34	-2.9318657188908688
3	35	-3.1753129384364414
3	36	-7.648285095170312
3	37	-5.38724943143939
3	38	-6.336157814504141
3	39	-3.082690018218102
3	40	-4.426480025797154
3	41	-2.4428479990105885
3	42	-1.8491460250064407
3	43	-2.0251684490829973
3	44	-1.8971645521667497
3	45	-2.1169985199215944
3	46	-1.3882525495118756
3	47	-1.7175218360934394
3	48	-1.6234241862278367
3	49	-1.4787039926762446
3	50	-1.7459483694437594
4	1	-0.10901939590633702
4	2	-0.1111060949960958
4	3	-0.10786036476023497
4	4	-0.05655823021805162
4	5	0.11580716934009416
4	6	-0.13946378061649511
4	7	-0.13420794517861623
4	8	-0.12178465613721556
4	9	-0.14579745071313965
4	10	-0.1874653128373467
4	11	-0.20537073248513593
4	12	-0.21244969938523894
4	13	-0.246202597486878
4	14	-0.21147903764224196
4	15	-0.20352290258003336
4	16	-0.25376721743956665
4	17	-0.3290467216081794
4	18	-0.2742418332872276
4	19	-0.2692074967020872
4	20	-0.2093934502571244
4	21	-0.2022910023998321
4	22	-0.17229399926696942
4	23	-0.12515887226582834
4	24	0.1169672340491772
4	25	-0.152979694691453
4	26	0.09389169732491354
4	27	0.2678944452938907
4	28	-0.05352895181110284
4	29	0.0011709217347615208
4	30	0.16055425724964323
4	31	0.18510469257330442
4	32	0.10560731364109444
4	33	0.15348630998872181
4	34	0.19069966109199754
4	35	-0.028326514519953605
4	36	0.11196264497039721
4	37	-0.14338164719462299
4	38	-0.371809859581397
4	39	-0.470755454722557
4	40	-0.47418858787076346
4	41	-0.617339426952455
4	42	-0.9909782127017195
4	43	-0.9060794829575016
4	44	-1.1356890460977098
4	45	-0.9989540130451857
4	46	-0.7515581155397086
4	47	-1.0432071434073293
4	48	-0.9921113647792481
4	49	-1.1513790383095355
4	50	-1.2165422454876165
5	1	-0.08973751816449038
5	2	-0.23838325478684652
5	3	-0.08745155974764884
5	4	-0.09483788019706196
5	5	0.1338968941908547
5	6	-0.1742968125331501
5	7	-0.15478415180352556
5	8	-0.17840798299115335
5	9	-0.14235589942415317
5	10	-0.14117887788905392
5	11	-0.20005388713746441
5	12	-0.21417535825069223
5	13	-0.18623988443183492
5	14	-0.17081824770573917
5	15	-0.22886315376732114
5	16	-0.1535656541800563
5	17	-0.17183414518608942
5	18	-0.19662317151766473
5	19	-0.18419726674490278
5	20	-0.10247614605025035
5	21	-0.2214722879707267
5	22	-0.25924829455412635
5	23	-0.9957190589455323
5	24	-0.7646734871213212
5	25	-0.12516477450866859
5	26	-0.0021227274953545816
5	27	0.14550192581412347
5	28	-0.23321272990555286
5	29	-0.15143857740760325
5	30	0.08276850736073271
5	31	0.12008675995750195
5	32	0.2000445253331975
5	33	0.23113943872036913
5	34	0.3448524843179257
5	35	0.10931131902589186
5	36	0.20873728661295132
5	37	-0.017112220103300047
5	38	-0.03123887402291814
5	39	-0.06856118383660714
5	40	-0.08890723374396003
5	41	-0.0571551679597917
5	42	-0.14832375285165966
5	43	-0.18230521548925793
5	44	-0.2055230540224855
5	45	-0.36954426052053435
5	46	-0.22473493991478222
5	47	-0.6802685706014415
5	48	-0.5551794692464469
5	49	-0.709129206110056
5	50	-0.9139160667017876
7	1	-0.06720530204644604
7	2	-0.09549360933694118
7	3	-0.10067185459362804
7	4	-0.040726142729297976
7	5	0.1440450150619057
7	6	-0.11238421111304575
7	7	-0.11386468819740259
7	8	-0.12411737870379369
7	9	-0.1356712668265061
7	10	-0.1721392718332595
7	11	-0.1875160187388829
7	12	-0.21046597540488676
7	13	-0.2070373762270563
7	14	-0.2548925593784688
7	15	-0.2062936894967109
7	16	-0.1593701829988845
7	17	-0.17892678780277294
7	18	-0.15009890443252516
7	19	-0.19151081044030605
7	20	-0.09455028123807807
7	21	-0.18624262019174154
7	22	-0.24214130830999636
7	23	-0.34049015738882105
7	24	-0.2674075894506416
7	25	-0.284200868007467
7	26	-0.16122556491970386
7	27	0.10579329151538275
7	28	-0.2509627963489507
7	29	-0.21155175858771713
7	30	0.017430751041636938
7	31	0.09463609849419896
7	32	0.04495678609381315
7	33	-0.17389080602264784
7	34	0.18080688995238603
7	35	-0.0516043286063379
7	36	-0.07860479607935389
7	37	-0.13987865269373856
7	38	-0.4085116207233952
7	39	-0.3838065738643337
7	40	-0.5159117403196376
7	41	-0.5491261848122022
7	42	-0.5443981626507011
7	43	-0.5647265940611419
7	44	-0.5193425349053413
7	45	-0.7016585780781739
7	46	-0.6667234828215953
7	47	-1.6169775259700145
7	48	-1.152330492568339
7	49	-1.0098075632538226
7	50	-1.039956546789682
8	1	-0.022369678660169257
8	2	-0.012119166868387765
8	3	-0.0414902775581254
8	4	-0.007639001005777857
8	5	0.23246745325990759
8	6	-0.08688528066750933
8	7	-0.07552852353537531
8	8	-0.09543306693437614
8	9	-0.1871481039932661
8	10	-0.09587275058635214
8	11	-0.14285458389964134
8	12	-0.17860382153574506
8	13	-0.20268990711306856
8	14	-0.15119650325453904
8	15	-0.14212625104959642
8	16	-0.19060113095060416
8	17	-0.13190689333862338
8	18	-0.17914290307065528
8	19	-0.18355620825859376
8	20	-0.10831670934732784
8	21	-0.07987408083622682
8	22	-0.12362156847595429
8	23	-0.050032815965046495
8	24	0.12907758859045515
8	25	-0.24231843773561224
8	26	0.0802026722348046
8	27	0.022331067853706088
8	28	-0.23515985807250672
8	29	-0.3848553223269846
8	30	-0.12026529351351911
8	31	-0.08659733269064418
8	32	-0.1607401718617112
8	33	0.049472440332067436
8	34	0.06669857782844701
8	35	-0.09060533009382965
8	36	-0.03637730319434951
8	37	-0.3862145775570198
8	38	-0.35914469652606584
8	39	-1.0307444703978206
8	40	-0.8990502750780109
8	41	-0.7426142954707302
8	42	-1.1660276457024494
8	43	-1.1891031999773531
8	44	-0.7134036997956912
8	45	-0.9248882242008776
8	46	-1.7469468430256114
8	47	-1.295727667722261
8	48	-1.9988974971855598
8	49	-2.3527783110303346
8	50	-1.6165412544511355
9	1	-0.039133235758917806
9	2	-0.07630179437943951
9	3	-0.08337093885950964
9	4	0.0026870150593722997
9	5	0.16276182508564574
9	6	-0.09629462962119106
9	7	-0.10924666912389824
9	8	-0.12777591995004656
9	9	-0.08822805894972419
9	10	-0.10078312471263473
9	11	-0.21822334829475665
9	12	-0.17778488026248596
9	13	-0.18649381457864678
9	14	-0.17574076348891765
9	15	-0.2128325317580675
9	16	-0.15781587408697817
9	17	-0.16678999263591265
9	18	-0.17638287269245012
9	19	-0.18370704007290328
9	20	-0.09999531356464786
9	21	-0.027999571237700138
9	22	-0.018007209687560533
9	23	0.05779377056713031
9	24	0.3112817530664387
9	25	0.07438060404659552
9	26	0.2925208025107864
9	27	0.4876799292882694
9	28	0.24366891391549927
9	29	0.2548006760503583
9	30	0.46708509233569373
9	31	0.5073231819039705
9	32	0.5045764590201074
9	33	0.6017441292547332
9	34	0.718590781078845
9	35	0.49414724151146316
9	36	0.590949685166791
9	37	0.4802992459264743
9	38	0.4538044837028181
9	39	0.4122917494208025
9	40	0.4166298283666734
9	41	0.27568476869646474
9	42	0.28724309861356545
9	43	0.29186895172417593
9	44	0.2767609566697402
9	45	0.2800173585028247
9	46	0.5169988446773885
9	47	0.3267875272173342
9	48	0.311337732055442
9	49	0.31643561460344927
9	50	0.313190490103743
10	1	0.02579245052621104
10	2	-0.0179144290645894
10	3	0.008210393372877434
10	4	0.05606776455853435
10	5	0.23984157696718
10	6	-0.07151569261864665
10	7	-0.13591145200331312
10	8	-0.10234191567261064
10	9	-0.157395227583649
10	10	-0.14863450743503392
10	11	-0.13933854470496243
10	12	-0.15303673810535656
10	13	-0.15676359096069348
10	14	-0.17956812284784007
10	15	-0.14854245446638714
10	16	-0.09617373049501765
10	17	-0.14979377600263888
10	18	-0.1256594476288342
10	19	-0.14553412193981785
10	20	-0.011737625005437735
10	21	-0.015414462406688756
10	22	0.004050782759243532
10	23	0.10789571504165707
10	24	0.3237797226370702
10	25	0.20139132132960924
10	26	0.3899886032850617
10	27	0.5583937774933645
10	28	0.3221848561700824
10	29	0.35905645600595903
10	30	0.585009553595107
10	31	0.6548685620003525
10	32	0.6298710404809356
10	33	0.7385795866427856
10	34	0.8596327805103243
10	35	0.5654385776693474
10	36	0.750209458300148
10	37	0.5828419357039394
10	38	0.6156978083278659
10	39	0.5618561377201132
10	40	0.5833686880775417
10	41	0.5345668819673506
10	42	0.42715457189311934
10	43	0.418084586707117
10	44	0.47402309186926317
10	45	0.4232607663754777
10	46	0.6086293302207366
10	47	0.39169164019042313
10	48	0.3624724036673328
10	49	0.3531553375175207
10	50	0.3685981157196352
11	1	-0.04264511426160311
11	2	-0.04997053474620117
11	3	-0.05994097014882982
11	4	0.006443403232808009
11	5	0.186214875051256
11	6	-0.11931578509410912
11	7	-0.05869721998568167
11	8	-0.07808498363973371
11	9	-0.07626200067198141
11	10	-0.08125036033165145
11	11	-0.13747546751524453
11	12	-0.12485191150699393
11	13	-0.1341545489257001
11	14	-0.1647408880124246
11	15	-0.13151166955904614
11	16	-0.11518320642214519
11	17	-0.10907227238403294
11	18	-0.1123976072828512
11	19	-0.1177681999907918
11	20	-0.05127693089970364
11	21	0.015684359201934228
11	22	0.016869843000741014
11	23	0.049272354597469814
11	24	0.3353300766037606
11	25	0.18318616198492776
11	26	0.3500797777281335
11	27	0.5454191757985143
11	28	0.3098746215791124
11	29	0.343574362129317
11	30	0.5678384487145465
11	31	0.5621023764151332
11	32	0.5226927027648429
11	33	0.6739342295803255
11	34	0.7723202020532085
11	35	0.5491638592413821
11	36	0.704627283289085
11	37	0.5485023428050155
11	38	0.5614477784152996
11	39	0.4652712442923175
11	40	0.42539072541123346
11	41	0.4687556328450313
11	42	0.4144647167853819
11	43	0.3980250353472812
11	44	0.3451433694079711
11	45	0.40480361997050096
11	46	0.5757877602843706
11	47	0.3631357768513155
11	48	0.35482662260299236
11	49	0.316238907538434
11	50	0.33846024023111454
12	1	-0.05478135503764037
12	2	-0.0671413464141466
12	3	-0.05489435488550498
12	4	-0.006185929853913308
12	5	0.16321013746454582
12	6	-0.08721431437352578
12	7	-0.10607389679207557
12	8	-0.0792620579553045
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112	12	5.54111987298711E-4
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118	3	-0.05943072634960345
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118	6	-0.11002173487963063
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119	1	-6.424211085933074
119	2	-7.357383920464667
119	3	-7.2384585473818746
119	4	-9.692942796177483
119	5	-30.059115614255255
119	6	-4.757190761747118
119	7	-5.857985571722757
119	8	-4.992900872295515
119	9	-5.111078394087102
119	10	-5.15031856934516
119	11	-1.8251290140836858
119	12	-1.7935408982223393
119	13	-1.7568064007309516
119	14	-1.7718472504914402
119	15	-1.7658117792930312
119	16	-1.909396005771022
119	17	-1.9985584473452036
119	18	-1.8917653145775546
119	19	-1.9257151340570928
119	20	-2.3411392014969437
119	21	-9.957680511355253
119	22	-11.736747499092342
119	23	-16.153840726173456
119	24	-29.428242126973323
119	25	-27.711396440982604
119	26	-32.789880120864666
119	27	-30.490232872668166
119	28	-22.752299466023445
119	29	-25.34252228146071
119	30	-24.67688576770649
119	31	-17.157176196421013
119	32	-15.51611729937851
119	33	-18.214519778680653
119	34	-14.345890684951748
119	35	-15.379144050647046
119	36	-12.377416617093687
119	37	-11.683613742906612
119	38	-11.421277176372296
119	39	-12.617423176549263
119	40	-11.72823246185493
119	41	-16.5810511893644
119	42	-12.449379171884576
119	43	-12.70572743596394
119	44	-13.904075901195306
119	45	-13.105164859031834
119	46	-19.328240913241533
119	47	-13.356980835038232
119	48	-13.452659063275831
119	49	-13.825986042431225
119	50	-13.789863626243417
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	26628.0
3	854.0
4	1559.0
5	1788.0
6	3185.0
7	4984.0
8	6245.0
9	7358.0
10	10599.0
11	14824.0
12	19270.0
13	26406.0
14	33932.0
15	36632.0
16	36939.0
17	36674.0
18	43991.0
19	62781.0
20	81708.0
21	70127.0
22	127355.0
23	294233.0
24	111651.0
25	111144.0
26	165235.0
27	207726.0
28	242168.0
29	241656.0
30	303858.0
31	411341.0
32	476830.0
33	483683.0
34	660256.0
35	970809.0
36	1455083.0
37	2516144.0
38	4681919.0
39	1.9620474E7
40	218963.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.77333692416652	20.059092073892508	11.528096746446387	22.639474255494584
2	17.41117719767859	22.881406137793075	37.00164826854941	22.70576839597893
3	18.661491591394473	27.29520124331407	31.589810533664636	22.45349663162682
4	11.604025209202634	16.253040617362245	38.72778358313173	33.41515059030339
5	12.06436170928235	41.78361953762008	35.07781444644203	11.074204306655536
6	29.815015881390885	38.342207109513545	18.172669226593232	13.670107782502338
7	27.3331998081376	33.7650010753459	22.711048273792674	16.190750842723826
8	28.864067831413458	34.48338768501055	20.605722843901585	16.04682163967441
9	28.39185737126294	12.389897162658055	19.783101150051326	39.435144316027674
10	16.496969936333723	28.55911128065346	35.19936670729298	19.74455207571984
11	35.27593864926645	21.67960622711814	23.759358349475264	19.285096774140147
12	20.01047860804259	27.38392323862362	32.86404368201366	19.74155447132014
13	29.565644166265702	20.708077911226685	27.37241764067131	22.3538602818363
14	20.86117153947857	20.33941691332359	29.81501292517353	28.984398622024315
15	23.20914490494002	29.499529237153116	24.892305737565756	22.39902012034111
16	21.716112270294612	27.3877522170105	27.535504139581402	23.360631373113485
17	20.752916309456943	28.572468737990363	28.857545747479822	21.81706920507287
18	20.89015368529817	26.889073831071396	30.132494400591625	22.088278083038805
19	21.94642167705194	26.51077520328592	30.935987328368032	20.606815791294107
20	22.452609766419805	25.830315725196186	30.812171645547647	20.904902862836362
21	22.636678640135287	25.86530551383019	30.201653637040128	21.2963622089944
22	22.206685000732552	25.892059280908402	30.487809564734835	21.41344615362421
23	22.142177636149746	25.96889570980721	30.331828386848713	21.557098267194334
24	21.42134472673636	26.59026528166158	30.30911143293794	21.679278558664112
25	21.810413390664863	25.965453198198198	30.82758461445718	21.396548796679767
26	21.54062532465947	26.289039626474793	30.50075357952492	21.669581469340823
27	21.442983979360225	26.47563807646519	30.340980262689065	21.740397681485515
28	21.54296646516042	26.228888655210458	30.77194927516775	21.456195604461374
29	21.54451314210147	26.405074629567054	30.642110785081318	21.408301443250163
30	21.408762031229863	26.507745107022114	30.345329790478516	21.73816307126951
31	21.64667790338418	26.42303201399886	30.520049307811025	21.410240774805935
32	21.785285001476176	26.388783090962768	30.44083216153916	21.385099746021893
33	21.659897274248532	26.5399409484931	30.32248942238255	21.477672354875818
34	22.001197735549507	26.120871906371224	30.284569386751375	21.59336097132789
35	21.89035279007086	26.186299901364663	30.57622951632369	21.34711779224079
36	22.037131960651895	26.654688467085496	29.762858593566072	21.545320978696537
37	21.95932716619986	26.31600701561407	30.203518868977124	21.52114694920895
38	22.002781456898806	26.746033158992976	29.841260114839045	21.409925269269173
39	21.824444230406	26.981521421195016	29.733892484284564	21.46014186411442
40	21.788750640621164	26.44084561786434	29.98621401891917	21.784189722595322
41	21.66774341387978	26.738524804659402	29.725525285439552	21.868206496021262
42	21.62331787741083	26.97075138308876	29.718653547134803	21.687277192365602
43	21.78829851035447	26.654851378121673	29.86674622894196	21.690103882581894
44	21.744278772710757	26.95513307773408	29.5203124109631	21.78027573859206
45	21.7395398119024	26.986078021454666	29.736992493632076	21.53738967301086
46	21.83987367110782	26.915856537572353	29.225591258782906	22.01867853253692
47	21.89360669512	27.001261974636883	29.386976670179216	21.718154660063902
48	22.024150473731627	27.342208653332484	28.656903874389826	21.976736998546063
49	21.936596821471667	27.107986848855887	28.97564757602525	21.979768753647196
50	21.990995561964873	27.249073075165015	28.85964509088173	21.90028627198838
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	13228.0
1	15655.0
2	18082.0
3	44874.0
4	71666.0
5	73865.5
6	76065.0
7	55167.5
8	34270.0
9	45979.0
10	57688.0
11	91828.0
12	125968.0
13	212070.0
14	298172.0
15	280263.5
16	262355.0
17	332623.0
18	402891.0
19	367298.0
20	331705.0
21	362030.5
22	392356.0
23	436502.0
24	480648.0
25	543097.0
26	605546.0
27	688826.0
28	772106.0
29	855979.0
30	939852.0
31	1048216.5
32	1156581.0
33	1261534.0
34	1366487.0
35	1478951.5
36	1591416.0
37	1699034.0
38	1806652.0
39	1875123.0
40	1943594.0
41	2000895.0
42	2058196.0
43	2113235.5
44	2168275.0
45	2209749.5
46	2251224.0
47	2192977.5
48	2134731.0
49	2083578.5
50	2032426.0
51	1981206.0
52	1929986.0
53	1854659.5
54	1779333.0
55	1647879.5
56	1516426.0
57	1397184.0
58	1277942.0
59	1203451.0
60	1128960.0
61	1004731.5
62	880503.0
63	768221.5
64	655940.0
65	565626.0
66	475312.0
67	394912.5
68	314513.0
69	265863.5
70	217214.0
71	171846.0
72	126478.0
73	99296.0
74	72114.0
75	52340.0
76	32566.0
77	23826.0
78	15086.0
79	10763.0
80	6440.0
81	4721.5
82	3003.0
83	2228.5
84	1454.0
85	1164.0
86	874.0
87	674.5
88	475.0
89	272.0
90	69.0
91	51.0
92	33.0
93	24.5
94	16.0
95	19.0
96	22.0
97	26.5
98	31.0
99	36.5
100	42.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3736185744102967
2	0.0
3	0.0
4	0.0
5	1.980665628994958E-4
6	0.0
7	0.0020309213240590094
8	2.6310334474709143E-4
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	2.956217356708893E-6
16	1.2120491162506461E-4
17	3.547460828050671E-5
18	6.621926879027919E-4
19	8.040911210248188E-4
20	0.0
21	0.0
22	0.0
23	0.0029650860087790196
24	0.006083895320106902
25	0.010018620621886437
26	0.009193835979364656
27	0.9782389292911831
28	0.03062641181550413
29	0.039820247794868785
30	1.0136987564849063
31	0.03452861872635987
32	0.029337501047979057
33	1.0240632545375277
34	1.9592714839844558
35	1.011191884166417
36	1.0398583238744232
37	1.0135213834435037
38	0.0386614105910389
39	0.034572961986710504
40	0.05329764272410462
41	0.045230125557646064
42	0.04864751282200154
43	1.0064589801783261
44	0.030369220905470458
45	0.03268689531313023
46	1.9911631568286317
47	0.044112675396810096
48	1.001099358110613
49	0.03954236336333815
50	0.052934027989229436
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	3.3827012E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.04018299197396
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.02724090200574	29.68170522271134
2	9.586482348327113	7.293430855593833
3	3.9839393583092653	4.546493466570353
4	2.1366843821941703	3.251194595790363
5	1.3351314292957275	2.539432194437261
6	0.8807255951809124	2.0101777683838256
7	0.6532711528133865	1.7395387937479307
8	0.48124502423927207	1.4645319028831083
9	0.36593916359345985	1.2528353472322598
>10	2.1500957676634203	15.341307739451421
>50	0.20979610249437794	5.5745054029081755
>100	0.1634698149589982	12.42731254552612
>500	0.016726977393723245	4.367959940526199
>1k	0.008889426433663286	6.045045268693007
>5k	2.7770177652169914E-4	0.7105581757380173
>10k+	8.485332037845424E-5	1.753970779806744
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	152983	0.45225099988139655	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	146730	0.43376577274989586	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	80894	0.23914024685360918	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	52396	0.15489396462211916	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	51201	0.15136128488085204	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	45915	0.1357347199332888	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.956217356708893E-6
2	0.0	0.0	0.0	0.0	2.956217356708893E-6
3	0.0	2.956217356708893E-6	0.0	0.0	2.956217356708893E-6
4	0.0	2.956217356708893E-6	0.0	0.0	2.956217356708893E-6
5	0.0	1.1824869426835572E-5	0.0	0.0	2.956217356708893E-6
6	2.956217356708893E-6	1.1824869426835572E-5	0.0	2.956217356708893E-6	2.956217356708893E-6
7	2.956217356708893E-6	1.1824869426835572E-5	0.0	2.956217356708893E-6	2.956217356708893E-6
8	2.956217356708893E-6	1.1824869426835572E-5	0.0	5.912434713417786E-6	2.956217356708893E-6
9	2.956217356708893E-5	1.1824869426835572E-5	0.0	5.912434713417786E-6	2.956217356708893E-6
10	2.956217356708893E-5	1.1824869426835572E-5	0.0	1.1824869426835572E-5	2.956217356708893E-6
11	3.2518390923797825E-5	1.1824869426835572E-5	0.0	1.7737304140253357E-5	2.956217356708893E-6
12	3.2518390923797825E-5	1.1824869426835572E-5	0.0	1.7737304140253357E-5	5.912434713417786E-6
13	3.2518390923797825E-5	1.7737304140253357E-5	0.0	1.7737304140253357E-5	5.912434713417786E-6
14	3.843082563721561E-5	1.7737304140253357E-5	0.0	1.7737304140253357E-5	5.912434713417786E-6
15	3.843082563721561E-5	2.069352149696225E-5	0.0	2.069352149696225E-5	5.912434713417786E-6
16	3.843082563721561E-5	2.069352149696225E-5	0.0	2.3649738853671143E-5	5.912434713417786E-6
17	3.843082563721561E-5	2.069352149696225E-5	0.0	2.956217356708893E-5	5.912434713417786E-6
18	3.843082563721561E-5	2.3649738853671143E-5	0.0	3.2518390923797825E-5	5.912434713417786E-6
19	6.503678184759565E-5	2.3649738853671143E-5	0.0	3.2518390923797825E-5	5.912434713417786E-6
20	6.503678184759565E-5	2.3649738853671143E-5	0.0	3.843082563721561E-5	5.912434713417786E-6
21	6.503678184759565E-5	2.6605956210380035E-5	0.0	4.13870429939245E-5	5.912434713417786E-6
22	6.503678184759565E-5	2.6605956210380035E-5	0.0	5.321191242076007E-5	5.912434713417786E-6
23	6.503678184759565E-5	2.6605956210380035E-5	0.0	6.208056449088675E-5	5.912434713417786E-6
24	7.094921656101343E-5	2.6605956210380035E-5	0.0	6.503678184759565E-5	5.912434713417786E-6
25	7.094921656101343E-5	2.6605956210380035E-5	0.0	6.799299920430454E-5	5.912434713417786E-6
26	7.686165127443122E-5	2.6605956210380035E-5	0.0	7.094921656101343E-5	5.912434713417786E-6
27	8.2774085987849E-5	2.6605956210380035E-5	0.0	9.755517277139346E-5	5.912434713417786E-6
28	8.2774085987849E-5	2.6605956210380035E-5	0.0	1.330297810519002E-4	5.912434713417786E-6
29	8.57303033445579E-5	2.6605956210380035E-5	0.0	2.1284764968304028E-4	5.912434713417786E-6
30	8.57303033445579E-5	2.6605956210380035E-5	0.0	2.985779530275982E-4	5.912434713417786E-6
31	8.57303033445579E-5	2.6605956210380035E-5	0.0	4.2273908200937167E-4	5.912434713417786E-6
32	8.57303033445579E-5	2.6605956210380035E-5	0.0	5.646375151313986E-4	5.912434713417786E-6
33	8.57303033445579E-5	2.6605956210380035E-5	0.0	7.893100342412744E-4	5.912434713417786E-6
34	9.755517277139346E-5	2.6605956210380035E-5	0.0	0.001215005333607355	5.912434713417786E-6
35	9.755517277139346E-5	2.6605956210380035E-5	0.0	0.0018121612396625514	5.912434713417786E-6
36	9.755517277139346E-5	2.6605956210380035E-5	0.0	0.002979867095562564	5.912434713417786E-6
37	9.755517277139346E-5	2.956217356708893E-5	0.0	0.005093562505609423	5.912434713417786E-6
38	9.755517277139346E-5	2.956217356708893E-5	0.0	0.008088210687955531	5.912434713417786E-6
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	58770	0.0	35.95877	1
TATGCCG	4260	0.0	29.39119	35
TCGTATG	4515	0.0	27.2115	32
GACCGAT	4770	0.0	26.060959	24
CGTATGC	4840	0.0	25.847462	33
TATCGTA	3615	0.0	24.310408	30
ATATCGT	3675	0.0	23.913027	29
GTATCAA	89835	0.0	23.58744	2
ACCGATA	3980	0.0	22.0306	25
AGACCGA	5995	0.0	21.592348	23
CGATATC	4065	0.0	21.517305	27
GATATCG	4145	0.0	21.311073	28
GCGGGCT	6420	0.0	20.939394	8
ATCGTAT	4395	0.0	20.149729	31
CCGATAT	4525	0.0	19.86784	26
CCGTCTT	6330	0.0	19.859903	39
GTACATG	214110	0.0	19.799438	1
TACATGG	214500	0.0	19.602205	2
GCCGTCT	6550	0.0	19.327126	38
ACATGGG	222020	0.0	18.651184	3
>>END_MODULE