##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR522120_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	26575823
Sequences flagged as poor quality	0
Sequence length	50
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.595657037601434	40.0	39.0	40.0	37.0	40.0
2	38.30388135110623	40.0	39.0	40.0	36.0	40.0
3	38.24734827591228	40.0	39.0	40.0	36.0	40.0
4	38.396114205005055	40.0	39.0	40.0	36.0	40.0
5	38.357066834769334	40.0	39.0	40.0	36.0	40.0
6	38.53037454380999	40.0	39.0	40.0	36.0	40.0
7	38.48628563638462	40.0	39.0	40.0	36.0	40.0
8	38.52608015187338	40.0	39.0	40.0	36.0	40.0
9	38.57021364869867	40.0	39.0	40.0	36.0	40.0
10	38.48733523699341	40.0	39.0	40.0	36.0	40.0
11	38.727898548993196	40.0	39.0	40.0	36.0	40.0
12	38.66339672716815	40.0	39.0	40.0	36.0	40.0
13	38.47961235292694	40.0	39.0	40.0	36.0	40.0
14	38.60154701511972	40.0	39.0	40.0	36.0	40.0
15	38.58808538121284	40.0	39.0	40.0	36.0	40.0
16	38.54393641920328	40.0	39.0	40.0	36.0	40.0
17	38.515008509802314	40.0	39.0	40.0	36.0	40.0
18	38.50227942141246	40.0	39.0	40.0	35.0	40.0
19	38.4363805403129	40.0	39.0	40.0	35.0	40.0
20	38.45623144013263	40.0	39.0	40.0	35.0	40.0
21	38.327924708107815	40.0	39.0	40.0	35.0	40.0
22	38.35409432099243	40.0	39.0	40.0	35.0	40.0
23	38.32953278624711	40.0	38.0	40.0	35.0	40.0
24	38.16082651513746	40.0	38.0	40.0	35.0	40.0
25	38.06557414985794	40.0	38.0	40.0	35.0	40.0
26	37.97837899507383	40.0	38.0	40.0	35.0	40.0
27	38.10749635110077	40.0	38.0	40.0	35.0	40.0
28	38.03191220079995	40.0	38.0	40.0	35.0	40.0
29	37.98247512410058	40.0	38.0	40.0	35.0	40.0
30	37.94844614972037	40.0	38.0	40.0	34.0	40.0
31	37.85031056234834	40.0	38.0	40.0	35.0	40.0
32	37.78843319358351	40.0	38.0	40.0	34.0	40.0
33	37.64262465173703	40.0	38.0	40.0	33.0	40.0
34	37.608361441901536	40.0	38.0	40.0	33.0	40.0
35	37.649444534605756	40.0	38.0	40.0	33.0	40.0
36	37.43241253525808	40.0	38.0	40.0	33.0	40.0
37	37.48596538289708	40.0	38.0	40.0	33.0	40.0
38	37.32049272754413	40.0	38.0	40.0	33.0	40.0
39	37.36173250401314	40.0	38.0	40.0	33.0	40.0
40	37.141495448701626	40.0	38.0	40.0	33.0	40.0
41	37.3922575041232	40.0	38.0	40.0	33.0	40.0
42	37.30521444246524	40.0	38.0	40.0	33.0	40.0
43	37.20616956998848	40.0	38.0	40.0	33.0	40.0
44	37.14501887674372	40.0	38.0	40.0	33.0	40.0
45	37.03333307871594	40.0	38.0	40.0	33.0	40.0
46	36.93499143939964	40.0	38.0	40.0	33.0	40.0
47	36.84740521488271	40.0	38.0	40.0	33.0	40.0
48	36.73855383519073	40.0	38.0	40.0	32.0	40.0
49	36.704156443245424	40.0	38.0	40.0	32.0	40.0
50	36.59715956868015	40.0	38.0	40.0	32.0	40.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	-6.704626154237431
1	2	-14.05773779217007
1	3	-9.121109820642705
1	4	-9.720216953144426
1	5	-15.182492605560615
1	6	-12.783663868207007
1	7	-18.00195321675776
1	8	-14.516791331001997
1	9	-13.338715617375406
1	10	-12.07497186963845
1	11	-15.047703517821986
1	12	-16.063764274763827
1	13	-17.47778479788572
1	14	-18.978580727771277
1	15	-23.389284397986245
1	16	-22.256198569337876
1	17	-25.64778549543385
1	18	-18.915882431312056
1	19	-21.571644296258732
1	20	-24.481575378396265
1	21	-18.807380998578772
1	22	-17.94695765038086
1	23	-22.10909297384209
1	24	-22.013378414431187
1	25	-19.48378453471707
1	26	-19.031975410060575
1	27	-20.42591487761806
1	28	-21.862747719764023
1	29	-19.115085990942006
1	30	-22.09752481078187
1	31	-17.514951147527015
1	32	-20.9547083035572
1	33	-18.2205547219573
1	34	-16.676990371486983
1	35	-17.334180603334648
1	36	-20.628597986656775
1	37	-16.766187943523594
1	38	-18.319853483318486
1	39	-15.661348137116871
1	40	-16.834213632899132
1	41	-19.386178262762275
1	42	-17.978851336555312
1	43	-18.031075730907432
1	44	-17.577184648398354
1	45	-17.272758932539244
1	46	-15.952223549121392
1	47	-16.84645333827431
1	48	-16.37604293985432
1	49	-16.663041316507666
1	50	-15.000000859006231
2	1	-15.518013120117253
2	2	-20.824464442605834
2	3	-17.7250334648331
2	4	-18.990542629726058
2	5	-21.14669288078026
2	6	-18.654215366037008
2	7	-18.766326386370686
2	8	-15.81210201164135
2	9	-16.26054618086999
2	10	-17.99737051471096
2	11	-14.333342697808579
2	12	-11.808356914402513
2	13	-12.086708265653268
2	14	-11.95616444041374
2	15	-13.966797541488592
2	16	-16.763396621629607
2	17	-15.473049247595032
2	18	-15.454282628905649
2	19	-14.52112737730739
2	20	-14.995283433863605
2	21	-17.0261100483653
2	22	-17.733090814348255
2	23	-14.194545145607378
2	24	-17.86931151488636
2	25	-17.807312286031422
2	26	-16.354501079585294
2	27	-16.78317114945039
2	28	-17.389433440098525
2	29	-16.725103280381337
2	30	-13.51572106356053
2	31	-15.1808944805075
2	32	-15.852143115314021
2	33	-19.54751919039525
2	34	-19.361990900755533
2	35	-19.16176960866262
2	36	-13.743862091663658
2	37	-15.335296302438241
2	38	-15.057615030194036
2	39	-14.948377529163729
2	40	-13.85251221093096
2	41	-13.760720445465775
2	42	-11.960104644483753
2	43	-14.204672287133395
2	44	-11.82521753361765
2	45	-13.639937225471744
2	46	-15.143342422837915
2	47	-14.964240290040301
2	48	-12.223610068748854
2	49	-15.069484279000168
2	50	-15.155736365869082
7	1	-0.11279320037498053
7	2	0.2059356644162733
7	3	0.13936107473823967
7	4	-0.14440875587040125
7	5	0.24577355453202898
7	6	-0.05097951525014821
7	7	-0.12389123011480763
7	8	-0.37691852607274257
7	9	-0.3785267227996343
7	10	-0.6393578446542065
7	11	-3.143214703087864
7	12	-3.6127109084772258
7	13	-21.397830080239366
7	14	-7.25527664627602
7	15	-5.525987302973064
7	16	-5.312552523337949
7	17	-5.532146698720204
7	18	-7.0981212743679265
7	19	-11.17147087793041
7	20	-4.458272973224105
7	21	-1.6033180510241607
7	22	-1.2116794578032355
7	23	-1.0559511523115575
7	24	-0.8300814329753763
7	25	-0.9685334823511695
7	26	-0.9973717212304862
7	27	-0.301788235193186
7	28	-1.8609860123370225
7	29	-1.2184479380291364
7	30	-0.4071265461547995
7	31	-0.15229316421400085
7	32	-0.030452178691675158
7	33	-0.5157097967145461
7	34	-0.009933851549959627
7	35	0.050015644351283584
7	36	0.360888790804033
7	37	0.22703395217612155
7	38	0.1679055187393388
7	39	0.14134441630443462
7	40	0.4514644303312352
7	41	0.20850873932582914
7	42	0.2991126122238228
7	43	0.24635536342602649
7	44	0.23166549940197
7	45	0.05764994699828918
7	46	0.057187977654763245
7	47	-0.0559307012882968
7	48	0.19951483637871803
7	49	0.15229117271587
7	50	0.2601412877850535
9	1	-0.7654008023346393
9	2	-0.42799297819747295
9	3	-0.4670609888834818
9	4	-0.6813611128346295
9	5	-0.5057194317346898
9	6	-0.13626366216666952
9	7	-0.07402595873346485
9	8	-0.20517021872193197
9	9	-0.24093790251576763
9	10	-0.10688691227210967
9	11	-0.043043121775497184
9	12	-0.04412999139974971
9	13	0.3730053876797328
9	14	0.06838112098875371
9	15	-0.09837942987083892
9	16	0.1551060812727485
9	17	0.19178289277237326
9	18	0.05876020195103848
9	19	0.1773473081438155
9	20	0.0862335504820706
9	21	0.32867561383590527
9	22	0.24366209988712484
9	23	0.12983454347116208
9	24	0.3014210737463259
9	25	0.557904260920516
9	26	0.4961882876979189
9	27	0.23888888236977834
9	28	0.28862109741143627
9	29	0.25916631807081814
9	30	0.24562499365862323
9	31	0.22411619706601726
9	32	0.3156966441925988
9	33	0.35368015210873693
9	34	0.2955114390651872
9	35	0.5280874398483846
9	36	0.7458273306741603
9	37	0.5751011894876683
9	38	0.7386061912887811
9	39	0.6177742840260052
9	40	0.7207490682360742
9	41	0.6662938736098099
9	42	0.746982486049319
9	43	0.7689132915334795
9	44	0.6842270955036369
9	45	0.7021737864481565
9	46	0.5597939474189388
9	47	0.6114493618078498
9	48	0.6149750325458143
9	49	0.6045634099027524
9	50	0.6631001375177092
10	1	0.5574094181512663
10	2	0.8836464861708748
10	3	0.8924010033148164
10	4	0.7683077952867521
10	5	0.8694609052633453
10	6	0.6336797135389389
10	7	0.6868411094833746
10	8	0.5355409255053729
10	9	0.5831810815960381
10	10	0.58213926622728
10	11	0.6122667766342147
10	12	0.5386886605152199
10	13	0.9171296130162148
10	14	0.5055045271394221
10	15	0.5569337149690838
10	16	0.2574890026052401
10	17	0.2563302076592393
10	18	0.17742862286343097
10	19	0.19444311230388678
10	20	0.13183073351937225
10	21	-2.316429307219373
10	22	-0.07744964844999913
10	23	-0.1532925950964099
10	24	-0.02602480706421062
10	25	0.21773490384815375
10	26	0.3154955342043948
10	27	0.1056710271014083
10	28	0.10105756039638436
10	29	0.006838928296048152
10	30	0.015610238046619429
10	31	0.30296649385280716
10	32	0.3538996203966036
10	33	0.3996145996423479
10	34	0.3689908397565631
10	35	0.3744288058963505
10	36	0.5152585355666872
10	37	0.2940326201390988
10	38	0.43056796408413334
10	39	0.2620917648548371
10	40	0.49890730579605247
10	41	0.2674038545003583
10	42	0.28961325814967864
10	43	0.23142176268731163
10	44	0.16136126523277738
10	45	0.10790350108743496
10	46	0.015058764940469871
10	47	-0.03294771278695663
10	48	-0.07059642839912073
10	49	-0.09030877779763102
10	50	-0.03738380059770918
11	1	0.352159889963346
11	2	0.6724474839075469
11	3	0.6443113830943403
11	4	0.5336118243902348
11	5	0.11940665650698179
11	6	0.4089275690912899
11	7	0.24016572883547838
11	8	0.008339410034665207
11	9	0.20636435307021372
11	10	0.3066333118481239
11	11	0.4382394123656965
11	12	0.38101864863907764
11	13	0.533137343268244
11	14	0.3704627010776136
11	15	0.4135236185758302
11	16	0.5885931732754699
11	17	0.649679983746168
11	18	0.5236254016250399
11	19	0.5971968820378919
11	20	0.5194000463226303
11	21	0.6939249962043661
11	22	0.5808383211942285
11	23	0.45193047548640664
11	24	0.5899561240272106
11	25	0.7664340539236321
11	26	0.6414718764913587
11	27	0.4028762867356903
11	28	0.4121384510375208
11	29	0.44205723128180097
11	30	0.3847131239995676
11	31	0.2785538296896064
11	32	0.2375097203533798
11	33	0.26785615904424276
11	34	0.2626787878593646
11	35	0.19400634488530955
11	36	0.43609385334370643
11	37	0.26247195991106054
11	38	0.3782910249566669
11	39	0.18437225332360185
11	40	0.2854901618918717
11	41	0.20240349044163963
11	42	-0.022435323374601523
11	43	0.05218543272224707
11	44	-0.14092464003513783
11	45	-0.19744315093797127
11	46	-0.1308783539500098
11	47	-0.46685730822184723
11	48	-0.38979787654516684
11	49	-0.39417949324113977
11	50	-0.5979873230214707
12	1	0.5926576447749241
12	2	0.953734771891213
12	3	0.9197460104224504
12	4	0.8507866497373797
12	5	0.9581068930060965
12	6	0.7610857458590843
12	7	0.7741259942273473
12	8	0.6868740739696548
12	9	0.6616482899106018
12	10	0.7101146706590384
12	11	0.6151147918561222
12	12	0.52352330558967
12	13	0.9554358439938184
12	14	0.6131680863477271
12	15	0.6904088017147387
12	16	0.6911290957276464
12	17	0.7655319387951494
12	18	0.661073227159207
12	19	0.6684192003212104
12	20	0.5662427378469062
12	21	0.7774133925566673
12	22	0.6838267095928359
12	23	0.6353030910671862
12	24	0.7648554122236462
12	25	0.9359186237635555
12	26	0.8237360998177081
12	27	0.6130763653948819
12	28	0.6999018647252342
12	29	0.616627855131199
12	30	0.5712059939199676
12	31	0.42798417157200674
12	32	0.4536599416633962
12	33	0.5371384353127979
12	34	0.4179898045512971
12	35	0.4683471545796465
12	36	0.5956493954196702
12	37	0.46063424483711657
12	38	0.6150426795562325
12	39	0.40158411198240884
12	40	0.5184140248033842
12	41	0.46904941016544655
12	42	0.5026376881956267
12	43	0.5213045796617308
12	44	0.46459517770986736
12	45	0.41157957611478935
12	46	0.437745538726773
12	47	0.44968495148624044
12	48	0.4043269077563849
12	49	0.40623971700899375
12	50	0.405816915293876
13	1	0.2030206392367404
13	2	0.35888301328315464
13	3	0.374411067511069
13	4	0.30520694982025987
13	5	-0.12694515298812803
13	6	0.521985080493593
13	7	0.537441816197159
13	8	0.42197442595863066
13	9	0.43878607422672644
13	10	0.4781669921012721
13	11	0.5995022060584247
13	12	0.511451874397892
13	13	0.9010436110058393
13	14	0.5618711179546096
13	15	0.6132216792510548
13	16	0.7192285915272194
13	17	0.7310281276020092
13	18	0.6199076095370017
13	19	0.6279662593385922
13	20	0.6301881481556251
13	21	0.7738735436862214
13	22	0.6693902432612262
13	23	0.5631424470535791
13	24	0.741189161941513
13	25	0.7593605922157423
13	26	0.7798898632271474
13	27	0.5167465764079537
13	28	0.6028080028633482
13	29	0.551813490300006
13	30	0.5472715704425966
13	31	0.40683794101269655
13	32	0.4334276761936948
13	33	0.4833231819596975
13	34	0.4189527906812742
13	35	0.3149725931668641
13	36	0.5693720907810089
13	37	0.4648392975359741
13	38	0.5405750558890929
13	39	0.4056330422430747
13	40	0.5052360325395568
13	41	0.4045530105755688
13	42	0.4299630383037254
13	43	0.4044331280756026
13	44	0.3820013777149711
13	45	0.32219182304304184
13	46	0.1674752203946852
13	47	0.12810446439716827
13	48	0.1811344653533098
13	49	0.1392813158758628
13	50	0.1755034890377729
15	1	-0.4152618098267169
15	2	-1.3344633195775373
15	3	-1.260086448871924
15	4	-1.0300891443586835
15	5	-0.8352050645469404
15	6	-0.5029666785081801
15	7	-0.4429769926424214
15	8	-0.5084774650776538
15	9	-0.5191308599292199
15	10	-0.6228380706415848
15	11	0.2401488189665173
15	12	0.1092092107028364
15	13	0.5509843800378462
15	14	0.09341682267690032
15	15	0.28566706949113296
15	16	0.28727506922577817
15	17	0.2894671208149475
15	18	0.20660153236121204
15	19	0.15862507672749615
15	20	0.044032696420757134
15	21	0.2642122825617008
15	22	0.14796217187120675
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119	40	-4.484233406686997
119	41	-4.966291105178364
119	42	-4.773899980073466
119	43	-4.84002402904342
119	44	-7.269918440483568
119	45	-5.048832649220408
119	46	-5.316356534436057
119	47	-5.069346111613971
119	48	-4.998740611820363
119	49	-5.0139891400727485
119	50	-6.211638392292109
120	1	-15.43488754207231
120	2	-13.478189344229463
120	3	-13.847867738789844
120	4	-16.420639428597216
120	5	-16.477624485867953
120	6	-14.260520317040424
120	7	-13.512248768599981
120	8	-14.096944033457419
120	9	-12.53048880777892
120	10	-13.537524578528533
120	11	-11.203113656830688
120	12	-10.420179828938611
120	13	-11.542574454978826
120	14	-11.560705552074978
120	15	-11.32170049106356
120	16	-17.772486697194513
120	17	-17.666949512831557
120	18	-13.457831066662052
120	19	-15.309804326179886
120	20	-17.61758100469953
120	21	-16.755498358680278
120	22	-18.417557903343315
120	23	-16.721025140491665
120	24	-14.538707235461256
120	25	-18.790849989255214
120	26	-12.986612284265043
120	27	-13.015294978843258
120	28	-13.087424629600406
120	29	-12.72542477563637
120	30	-12.875343140304683
120	31	-11.061652983616188
120	32	-11.068656428156292
120	33	-11.258004426063614
120	34	-11.115432721986775
120	35	-10.556437277783228
120	36	-10.323224846926188
120	37	-10.475657345723842
120	38	-10.414511109291979
120	39	-10.439782685594974
120	40	-10.037925424609284
120	41	-9.771093516317926
120	42	-9.836251694885277
120	43	-9.728331751836151
120	44	-9.693218305676456
120	45	-9.565826092261506
120	46	-9.584228226312742
120	47	-9.702807260499416
120	48	-9.624735196347793
120	49	-8.63715398735284
120	50	-9.766463728018735
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	55442.0
3	2745.0
4	2345.0
5	1893.0
6	3647.0
7	5432.0
8	6106.0
9	6214.0
10	7318.0
11	8752.0
12	9544.0
13	10179.0
14	11249.0
15	12542.0
16	13677.0
17	15618.0
18	18883.0
19	24659.0
20	33746.0
21	38205.0
22	53629.0
23	79096.0
24	107748.0
25	110963.0
26	154417.0
27	189135.0
28	200205.0
29	188654.0
30	201511.0
31	234816.0
32	298655.0
33	363271.0
34	519663.0
35	743925.0
36	1067077.0
37	1779626.0
38	3454362.0
39	1.620647E7
40	334404.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.64934805400851	19.99028283278906	11.348163741641027	23.0122053715614
2	17.66084158522579	22.464579930412693	36.587540487457346	23.28703799690418
3	18.45396471823281	27.052573310711768	31.289190931170786	23.204271039884635
4	11.754533434392606	16.25262179086608	38.52400356519533	33.468841209545985
5	12.15313632996427	41.18451195283774	35.28013036510666	11.382221352091335
6	30.128075431568007	38.44783659192794	17.811689218429848	13.61239875807421
7	27.583800509207183	33.47196058613124	22.7814581697056	16.162780734955977
8	28.755485766141653	34.44759923333324	20.371109485489875	16.425805515035226
9	28.371298228468785	12.664627545118734	19.607411593612735	39.35666263279975
10	16.74097858436568	28.049744320193643	35.207382892221794	20.001894203218882
11	35.33740422639028	21.837400858667667	23.50225240437521	19.32294251056684
12	20.350022650286313	27.27986636575658	32.255234391047836	20.11487659290928
13	29.51882572238272	20.617294826726706	27.010864392864114	22.853015058026457
14	21.174301920960264	20.260482619860916	29.221601152295452	29.343614306883364
15	23.39888777856475	29.29791111266808	24.614917852214774	22.688283256552396
16	22.195775703974395	27.026299679552483	27.28617670154935	23.491747914923764
17	21.15801644223774	28.231607352291594	28.397724503207293	22.21265170226337
18	21.11335067454316	26.928948702449645	29.66665382281261	22.291046800194582
19	22.256902908364985	26.296647200453975	30.5327732295908	20.91367666159024
20	22.708366438309902	25.76742619313283	30.322662189111583	21.201545179445684
21	22.836542070587992	25.81278480068143	29.715975305825896	21.634697822904673
22	22.35143573916789	25.734439155468486	30.093540282835267	21.820584822528353
23	22.314207205476137	25.989601243780474	30.015773800183027	21.68041775056036
24	21.611834725139353	26.643040948577074	29.74730511548451	21.99781921079906
25	22.092655181164734	26.037412273004097	30.29245568155443	21.577476864276747
26	21.675316413968005	26.36084875200087	30.063131514593383	21.900703319437742
27	21.58885230131019	26.462035504713498	29.8835353261248	22.06557686785151
28	21.7837179859594	26.068636407877086	30.605810102510723	21.541835503652795
29	21.876314389386806	26.401215081639567	30.231412338912065	21.49105819006156
30	21.604878395987328	26.46517703016789	30.013526717553802	21.91641785629098
31	21.906859091627705	26.421667381861162	30.160802071805527	21.510671454705605
32	21.975641201791056	26.32695597837032	30.116712183351623	21.580690636487002
33	21.77691589997039	26.5689230477925	29.898303145128306	21.755857907108805
34	22.41973977633594	25.894780469221722	29.846966893081227	21.838512861361107
35	22.14383749501857	26.08516835958376	30.274700027163725	21.496294118233937
36	22.317692339897597	26.74134583034517	29.22206748880362	21.718894340953607
37	22.173854110502997	26.100304676934012	29.928525923895528	21.797315288667463
38	22.305069923192768	26.710093857435456	29.4443904679817	21.540445751390067
39	22.032120084425895	26.981653893743378	29.2642359528272	21.721990069003525
40	22.03079419149219	26.390845658329816	29.527164899999235	22.05119525017876
41	22.07704718307147	26.53030631631259	29.301090681273077	22.091555819342858
42	21.698587003753577	27.02690858853452	29.30725769368358	21.96724671402832
43	22.089247932062957	26.55782984312056	29.441873653622608	21.911048571193874
44	21.923379058661354	26.89268860177863	29.116754291391683	22.06717804816833
45	21.90500712302592	26.84154923414061	29.515063943485583	21.73837969934789
46	22.137711067850375	26.68975595832438	28.72122737211206	22.45130560171318
47	22.155458363099893	26.842077202965754	29.136082130628775	21.866382303305574
48	22.29728142435901	27.260038403312485	28.1300800397797	22.312600132548802
49	22.18726868080237	27.029712129123595	28.663120034278865	22.11989915579517
50	22.15076024379065	27.040947753800694	28.67352147459894	22.134770527809717
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	28087.0
1	20706.5
2	13326.0
3	29736.0
4	46146.0
5	47305.0
6	48464.0
7	36371.5
8	24279.0
9	31653.5
10	39028.0
11	60720.0
12	82412.0
13	138371.0
14	194330.0
15	189252.0
16	184174.0
17	231229.5
18	278285.0
19	254936.5
20	231588.0
21	249163.0
22	266738.0
23	295268.5
24	323799.0
25	361685.0
26	399571.0
27	459439.0
28	519307.0
29	584697.5
30	650088.0
31	740620.0
32	831152.0
33	926486.0
34	1021820.0
35	1130823.0
36	1239826.0
37	1336666.0
38	1433506.0
39	1501184.5
40	1568863.0
41	1622955.0
42	1677047.0
43	1736476.5
44	1795906.0
45	1849046.0
46	1902186.0
47	1836269.0
48	1770352.0
49	1716630.5
50	1662909.0
51	1617118.5
52	1571328.0
53	1496548.5
54	1421769.0
55	1311436.0
56	1201103.0
57	1114245.5
58	1027388.0
59	958555.0
60	889722.0
61	794212.0
62	698702.0
63	607043.5
64	515385.0
65	445501.0
66	375617.0
67	313513.0
68	251409.0
69	211643.5
70	171878.0
71	137359.5
72	102841.0
73	81132.5
74	59424.0
75	43994.5
76	28565.0
77	21067.5
78	13570.0
79	10124.5
80	6679.0
81	5081.5
82	3484.0
83	2643.0
84	1802.0
85	1411.0
86	1020.0
87	864.0
88	708.0
89	393.5
90	79.0
91	56.5
92	34.0
93	28.0
94	22.0
95	20.0
96	18.0
97	27.0
98	36.0
99	43.5
100	51.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.48857941294988305
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	1.881409279404066E-5
11	0.0
12	0.0
13	0.010106930648958642
14	0.0
15	0.0
16	6.622560663502311E-4
17	0.0
18	7.789034416732833E-4
19	1.2041019388186021E-4
20	3.762818558808132E-5
21	0.0
22	0.0
23	0.009952655088047508
24	0.007363835919587514
25	0.005023362776008856
26	0.009057104271051173
27	0.024055699046460387
28	0.023739622287520506
29	0.0439572464039966
30	0.03161520153110592
31	0.036209602991410655
32	0.03940799876639756
33	0.04172213218006456
34	0.04132703623138971
35	0.05519302262059768
36	0.05812425827790921
37	0.06224830741836292
38	0.040435248232952185
39	0.03955851150874989
40	0.05070774289849838
41	0.046410604104339497
42	0.05256281244799079
43	0.048453814581772316
44	0.04648586047551566
45	0.03976546652948434
46	0.04838984666627257
47	0.06162367953760077
48	0.05091469791923283
49	0.05588538123541837
50	0.05644604120068078
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2.6575823E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.5908141502369
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.2205882607529	59.5660287717581
2	3.965084994659998	5.12216135959954
3	1.4004943865660555	2.7137721792341436
4	0.6683013850219216	1.7266452222518742
5	0.39969029046122073	1.2908160634417456
6	0.2694572112669725	1.0442676392631685
7	0.18186880146603665	0.8222937768653373
8	0.12506940324365598	0.6462667664633617
9	0.09707409732875762	0.5643085481427412
>10	0.5683739590360636	6.707039834276342
>50	0.05050626013045113	2.299992548820621
>100	0.041576268216107236	5.481451408806447
>500	0.00638934169212464	2.85201291217812
>1k	0.005222500327139674	6.575869931508584
>5k	2.3125950714650027E-4	0.9850291686812814
>10k+	7.158032363001797E-5	1.602043868708445
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	98252	0.36970444904001654	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	95689	0.3600603450737913	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	55406	0.20848272506932336	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	32648	0.1228485003079679	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	31836	0.11979309163821568	No Hit
TCTCTGAGCGGGCTGGCAAGGCAGACCGATATCGTATGCCGTCTTCTGCT	30406	0.11441226109912006	Illumina PCR Primer Index 1 (95% over 24bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	27449	0.1032856066207244	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	3.7628185588081317E-6	0.0	0.0	0.0
4	0.0	7.525637117616263E-6	0.0	3.7628185588081317E-6	0.0
5	3.7628185588081317E-6	1.1288455676424395E-5	0.0	1.1288455676424395E-5	0.0
6	3.7628185588081317E-6	1.1288455676424395E-5	0.0	1.5051274235232527E-5	0.0
7	3.7628185588081317E-6	1.1288455676424395E-5	0.0	1.881409279404066E-5	0.0
8	3.7628185588081317E-6	1.1288455676424395E-5	0.0	1.881409279404066E-5	0.0
9	3.7628185588081317E-6	1.1288455676424395E-5	0.0	3.762818558808132E-5	0.0
10	3.7628185588081317E-6	1.1288455676424395E-5	0.0	4.139100414688945E-5	0.0
11	3.7628185588081317E-6	1.1288455676424395E-5	0.0	4.515382270569758E-5	7.525637117616263E-6
12	3.7628185588081317E-6	1.1288455676424395E-5	0.0	4.891664126450571E-5	7.525637117616263E-6
13	3.7628185588081317E-6	1.1288455676424395E-5	0.0	5.267945982331385E-5	7.525637117616263E-6
14	3.7628185588081317E-6	1.1288455676424395E-5	0.0	5.644227838212197E-5	7.525637117616263E-6
15	3.7628185588081317E-6	1.1288455676424395E-5	0.0	6.396791549973824E-5	7.525637117616263E-6
16	3.7628185588081317E-6	1.1288455676424395E-5	0.0	7.14935526173545E-5	7.525637117616263E-6
17	3.7628185588081317E-6	1.1288455676424395E-5	0.0	8.27820082937789E-5	7.525637117616263E-6
18	3.7628185588081317E-6	1.1288455676424395E-5	0.0	8.27820082937789E-5	7.525637117616263E-6
19	3.7628185588081317E-6	1.881409279404066E-5	0.0	9.030764541139516E-5	7.525637117616263E-6
20	3.7628185588081317E-6	1.881409279404066E-5	0.0	9.40704639702033E-5	7.525637117616263E-6
21	3.7628185588081317E-6	2.6339729911656923E-5	0.0	1.1288455676424395E-4	7.525637117616263E-6
22	3.7628185588081317E-6	2.6339729911656923E-5	0.0	1.316986495582846E-4	7.525637117616263E-6
23	3.7628185588081317E-6	2.6339729911656923E-5	0.0	1.42987105234709E-4	1.5051274235232527E-5
24	3.7628185588081317E-6	2.6339729911656923E-5	0.0	1.768524722639822E-4	1.5051274235232527E-5
25	3.7628185588081317E-6	2.6339729911656923E-5	0.0	2.0319220217563912E-4	1.5051274235232527E-5
26	7.525637117616263E-6	2.6339729911656923E-5	0.0	2.144806578520635E-4	1.5051274235232527E-5
27	7.525637117616263E-6	2.6339729911656923E-5	0.0	2.3329475064610416E-4	1.5051274235232527E-5
28	7.525637117616263E-6	2.6339729911656923E-5	0.0	2.972626661458424E-4	1.5051274235232527E-5
29	7.525637117616263E-6	2.6339729911656923E-5	0.0	3.838074929984294E-4	1.5051274235232527E-5
30	7.525637117616263E-6	2.6339729911656923E-5	0.0	5.042176868802897E-4	1.5051274235232527E-5
31	1.1288455676424395E-5	2.6339729911656923E-5	0.0	6.321535178797661E-4	1.5051274235232527E-5
32	1.1288455676424395E-5	2.6339729911656923E-5	0.0	8.052431715849402E-4	1.5051274235232527E-5
33	1.1288455676424395E-5	2.6339729911656923E-5	0.0	0.001113794293407207	1.5051274235232527E-5
34	1.1288455676424395E-5	2.6339729911656923E-5	0.0	0.001715845262816508	1.5051274235232527E-5
35	1.1288455676424395E-5	2.6339729911656923E-5	0.0	0.002630210172606884	1.881409279404066E-5
36	1.1288455676424395E-5	2.6339729911656923E-5	0.0	0.0043084272498353105	1.881409279404066E-5
37	1.5051274235232527E-5	2.6339729911656923E-5	0.0	0.007284816729852543	1.881409279404066E-5
38	1.5051274235232527E-5	2.6339729911656923E-5	0.0	0.011630872165275936	1.881409279404066E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	40585	0.0	35.882286	1
TATGCCG	6570	0.0	31.818733	35
GACCGAT	6740	0.0	30.91428	24
TCGTATG	6775	0.0	30.856186	32
CGTATGC	7075	0.0	29.765123	33
ATATCGT	4985	0.0	29.530449	29
TATCGTA	5120	0.0	28.707651	30
ATCGTAT	5300	0.0	27.94262	31
AGACCGA	7790	0.0	27.256414	23
ACCGATA	5535	0.0	27.071457	25
GATATCG	5565	0.0	26.887094	28
GCCGTCT	7980	0.0	26.773895	38
GCGGGCT	8280	0.0	26.664669	8
CGATATC	5675	0.0	26.444927	27
CCGATAT	5745	0.0	26.273989	26
CCGTCTT	8260	0.0	25.813078	39
CGGGCTG	9180	0.0	24.313988	9
TGAGCGG	9620	0.0	23.359596	5
CAGACCG	9300	0.0	23.228214	22
ATGCCGT	9280	0.0	22.763924	36
>>END_MODULE