FastQCFastQC Report
Thu 26 May 2016
SRR522119_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR522119_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28662645
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1874310.6539208087739286No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1423620.49668130767415214No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT910610.31769922140821266No Hit
TCTCTGAGCGGGCTGGCAAGGCAGACCGATATCGTATGCCGTCTTCTGCT695050.24249332188288975Illumina PCR Primer Index 1 (95% over 24bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT344950.12034827909287507No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA920650.037.846131
TATGCCG146750.033.2522935
GCCGTCT148750.032.22130238
CCGTCTT150500.031.77388639
GACCGAT156150.031.40593524
TCGTATG156000.031.30280332
CGTATGC157050.031.12556633
ATGCCGT153900.031.02721836
TATCGTA109650.030.80469530
ATATCGT110200.030.629729
ACCGATA112000.030.5911725
ATCGTAT113750.030.33534831
AGACCGA169000.030.30551723
TGCCGTC159100.030.09641637
CGATATC113900.029.96589527
GATATCG116550.029.28532628
CGTCTTC164650.029.0980540
CCGATAT118350.028.83886526
GCGGGCT183850.028.7735658
CGGGCTG189200.028.0296639
Produced by FastQC (version 0.11.5)