##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR522119_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	28662645
Sequences flagged as poor quality	0
Sequence length	50
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.83289595220539	40.0	39.0	40.0	37.0	40.0
2	38.726223417273594	40.0	39.0	40.0	37.0	40.0
3	38.62412327962056	40.0	39.0	40.0	36.0	40.0
4	38.72082771844678	40.0	39.0	40.0	36.0	40.0
5	38.74507931839508	40.0	39.0	40.0	37.0	40.0
6	38.78466006888059	40.0	39.0	40.0	37.0	40.0
7	38.73645739951773	40.0	39.0	40.0	36.0	40.0
8	38.699314770147694	40.0	39.0	40.0	36.0	40.0
9	38.681808221118466	40.0	39.0	40.0	36.0	40.0
10	38.62291808728748	40.0	39.0	40.0	36.0	40.0
11	38.65563342113053	40.0	39.0	40.0	36.0	40.0
12	38.54493721706423	40.0	38.0	40.0	36.0	40.0
13	38.529926983361094	40.0	38.0	40.0	35.0	40.0
14	38.46994194011055	40.0	38.0	40.0	35.0	40.0
15	38.45363601300578	40.0	38.0	40.0	35.0	40.0
16	38.30385227881097	40.0	38.0	40.0	35.0	40.0
17	38.283388186959016	40.0	38.0	40.0	35.0	40.0
18	38.23537977740715	40.0	38.0	40.0	35.0	40.0
19	38.16763582007174	40.0	38.0	40.0	35.0	40.0
20	38.11932813597628	40.0	38.0	40.0	35.0	40.0
21	38.35741593282825	40.0	38.0	40.0	35.0	40.0
22	38.35714362718444	40.0	38.0	40.0	35.0	40.0
23	38.325367355315606	40.0	38.0	40.0	35.0	40.0
24	38.234111157571114	40.0	38.0	40.0	35.0	40.0
25	38.19653768868854	40.0	38.0	40.0	35.0	40.0
26	38.171666466929345	40.0	38.0	40.0	35.0	40.0
27	38.058278536401644	40.0	38.0	40.0	35.0	40.0
28	37.995979261509184	40.0	38.0	40.0	35.0	40.0
29	37.91863165454549	40.0	38.0	40.0	35.0	40.0
30	37.80296626497659	40.0	38.0	40.0	34.0	40.0
31	37.460659335521896	40.0	38.0	40.0	33.0	40.0
32	37.37879417618297	40.0	38.0	40.0	33.0	40.0
33	37.2518499950022	39.0	38.0	40.0	33.0	40.0
34	37.074709120529526	39.0	38.0	40.0	33.0	40.0
35	36.85578532616233	39.0	38.0	40.0	32.0	40.0
36	36.52113411026791	39.0	37.0	40.0	32.0	40.0
37	36.33780818204321	39.0	37.0	40.0	31.0	40.0
38	36.26517622501343	39.0	37.0	40.0	31.0	40.0
39	36.06837742992665	39.0	36.0	40.0	30.0	40.0
40	35.86140797543283	39.0	36.0	40.0	30.0	40.0
41	35.410064039798144	39.0	36.0	40.0	29.0	40.0
42	35.17779719910706	39.0	36.0	40.0	27.0	40.0
43	34.93899031300147	39.0	35.0	40.0	27.0	40.0
44	34.61282763680742	39.0	35.0	40.0	24.0	40.0
45	34.16280343283043	39.0	35.0	40.0	20.0	40.0
46	33.369259326904405	39.0	35.0	40.0	2.0	40.0
47	33.15385774760145	38.0	34.0	40.0	2.0	40.0
48	32.85045661347723	38.0	33.0	40.0	2.0	40.0
49	32.64901616720997	38.0	33.0	40.0	2.0	40.0
50	32.439966897681636	38.0	33.0	40.0	2.0	40.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	-0.35380196772174344
1	2	-0.2507185648196284
1	3	-0.24467592785646275
1	4	-0.2927126156839748
1	5	-0.3496740562955196
1	6	-0.3663424245997504
1	7	-0.347670514856226
1	8	-0.3653957831709249
1	9	-0.4458422699315605
1	10	-0.30617026198938646
1	11	-0.394561326997632
1	12	-0.32407121888746104
1	13	-0.3433607153790419
1	14	-0.2516227875497634
1	15	-0.30010577849768794
1	16	-0.22154456632627273
1	17	-0.16473265279397964
1	18	-0.27666530477889495
1	19	-0.29625191946160356
1	20	-0.22164672019957266
1	21	-0.17257232246745957
1	22	-0.1429773909676797
1	23	-0.13329250564835604
1	24	-0.07653537257385068
1	25	-0.09241725416417523
1	26	-0.040612803011676135
1	27	-0.07036977076299422
1	28	-0.05218958651688155
1	29	-0.21921391402295853
1	30	0.004339450456576799
1	31	0.09794928173539574
1	32	0.07766140498876695
1	33	0.10785617647047019
1	34	-0.030671168416049
1	35	0.13427037151028287
1	36	0.298168101989539
1	37	0.18560140396908764
1	38	0.2454279454588999
1	39	0.11310711678095942
1	40	0.302838530301365
1	41	0.32944227950061844
1	42	0.34024241822914547
1	43	0.24375709337118678
1	44	0.2840733586387145
1	45	0.33427019831794524
1	46	0.12346816157358376
1	47	0.3573498500074166
1	48	0.3973517381421985
1	49	0.3334354989091608
1	50	0.37498369023737865
2	1	-0.23138453534701853
2	2	-0.24069102110257035
2	3	-0.20966616976647146
2	4	-0.22697421084148317
2	5	-0.26793391309873016
2	6	-0.2893707108751684
2	7	-0.27419799727737626
2	8	-0.2714021387627028
2	9	-0.3370308575933194
2	10	-0.40096570387772346
2	11	-0.3483623330327603
2	12	-0.28751356150339547
2	13	-0.28755580850389606
2	14	-0.2323235013109226
2	15	-0.30602092165604233
2	16	-0.19626777654666938
2	17	-0.17752556301388722
2	18	-0.2738749031913841
2	19	-0.3244291598359226
2	20	-0.20905988574862278
2	21	-0.17329944409328135
2	22	-0.13823903390763093
2	23	-0.15305548582195172
2	24	-0.1148920955971704
2	25	-0.12426102305393272
2	26	-0.07868290715207138
2	27	-0.15232207349246352
2	28	-0.09599562619894186
2	29	-0.21025711993868157
2	30	-0.05496091065268871
2	31	0.01761143164411294
2	32	-0.1383204777793523
2	33	-0.0021459720435217378
2	34	-0.11910414452564311
2	35	0.009605143599614507
2	36	0.18428414776914792
2	37	0.10027007566529988
2	38	0.13202208630080037
2	39	0.19523607598776493
2	40	0.2051751098169987
2	41	0.23217652282499301
2	42	0.2273731751773056
2	43	0.09854575767248264
2	44	0.1271618880312957
2	45	0.18132205079910335
2	46	-0.047661469005056745
2	47	0.168231962622734
2	48	0.2953091011197273
2	49	0.19100532729196118
2	50	0.24977060966039488
3	1	-0.18530670855144393
3	2	-0.295855550341777
3	3	-0.1926036303555918
3	4	-0.21836319138038363
3	5	-0.20090734635662244
3	6	-0.19033737766402936
3	7	-0.23975991232433813
3	8	-0.21998254148940788
3	9	-0.3295748750326837
3	10	-0.21824991234110058
3	11	-0.23386493189316582
3	12	-0.23537895691561772
3	13	-0.28798357296705746
3	14	-0.20938237602566545
3	15	-0.24882228941696383
3	16	-0.25641529650197725
3	17	-0.19774598207477823
3	18	-0.2021003999762101
3	19	-0.1788861240210835
3	20	-0.2573086602032646
3	21	-0.14376030416973862
3	22	-0.16470080073931825
3	23	-0.17470525623286903
3	24	-0.18296583088706342
3	25	-0.1553259986477471
3	26	-0.18969300174193648
3	27	-0.2455273260134021
3	28	-0.12099414677310705
3	29	-0.06743018800454337
3	30	-0.11768160464516342
3	31	-0.036069556724790175
3	32	-0.1192831772987546
3	33	-0.015935709806278453
3	34	-0.05283235107012274
3	35	0.06492110102069404
3	36	0.09084889004689956
3	37	0.19068677800166967
3	38	-0.04015630468931164
3	39	0.09915053651330652
3	40	0.09586277919778041
3	41	0.049563665042626326
3	42	0.05613490271346677
3	43	-0.03648020420352793
3	44	0.16065736131680808
3	45	0.025407914096433615
3	46	0.13488193153567352
3	47	0.106811089848037
3	48	0.21138108191745886
3	49	0.19124312817431388
3	50	0.12460577945990536
4	1	-0.13675707922077152
4	2	-0.17255936293162932
4	3	-0.20268637141107604
4	4	-0.21673643964000178
4	5	-0.11352578079436171
4	6	-0.17512815917848457
4	7	-0.27371499363844265
4	8	-0.214723539660163
4	9	-0.28564112044311685
4	10	-0.20607729993577095
4	11	-0.251689038856874
4	12	-0.2372406681397976
4	13	-0.33686321288563903
4	14	-0.2230791489376216
4	15	-0.25923072884572207
4	16	-0.1570938631987886
4	17	-0.17804394561267856
4	18	-0.18587643818872124
4	19	-0.0864996974736485
4	20	-0.12968714662941494
4	21	-0.18234520621688688
4	22	-0.06332416619198966
4	23	-0.168239292760326
4	24	-0.19167089306977658
4	25	-0.1231026512634017
4	26	-0.06740936405061149
4	27	-0.2697229924275746
4	28	-0.16128187267375438
4	29	-0.06596861332216974
4	30	-0.13841420044322206
4	31	-0.06682592166976775
4	32	-0.1332452675855862
4	33	-0.06609049982353099
4	34	-0.11098680765275049
4	35	0.043508415759418995
4	36	0.20300034611764062
4	37	0.18034704794180811
4	38	0.076527535051504
4	39	0.11089149023776912
4	40	-0.011706211801708832
4	41	0.1634017928998901
4	42	-0.014901346946970762
4	43	0.180944615669695
4	44	0.038232167560082075
4	45	0.11653218964875123
4	46	0.04691837439631996
4	47	0.032927320721611864
4	48	0.06794557050017147
4	49	0.06106157069682894
4	50	0.12576051483745943
5	1	-0.38878409174090933
5	2	-0.5425768671634685
5	3	-0.3419284573352144
5	4	-0.4731210347120509
5	5	-0.4269799104165699
5	6	-0.3239159758237875
5	7	-0.2881136364409471
5	8	-0.27363765525250017
5	9	-0.28396058576694116
5	10	-0.304543369597134
5	11	-0.25476052137612015
5	12	-0.325020663612996
5	13	-0.30774400472262897
5	14	-0.27475577583692967
5	15	-0.2924372759722047
5	16	-0.3165112693499381
5	17	-0.2620930274907849
5	18	-0.3047897421272836
5	19	-0.3011838889059604
5	20	-0.32882409981154126
5	21	-0.17868157761681402
5	22	-0.22232453881029812
5	23	-0.13563508504277166
5	24	-0.13248805887509008
5	25	-0.07212988069274928
5	26	-0.01683158494405035
5	27	-0.11827074415663219
5	28	-0.06456475639035375
5	29	-0.006042403586818068
5	30	-0.06798562718007872
5	31	0.05693221794351189
5	32	0.01830481054988553
5	33	0.08348213285627537
5	34	0.12317188260090006
5	35	0.20435123744921668
5	36	0.34556078171787874
5	37	0.33446326632945045
5	38	0.26526646585967484
5	39	0.30879154425438315
5	40	0.19082539052526215
5	41	0.3282380465147128
5	42	0.20987881426813715
5	43	0.3676983205832798
5	44	0.25668822649036116
5	45	0.3729811953176849
5	46	0.34526652852105144
5	47	0.3275761946799065
5	48	0.31196961150464375
5	49	0.3359942004229808
5	50	0.3790864643620253
6	1	-0.15015051157338632
6	2	-0.12186056989861527
6	3	-0.11081235166921743
6	4	-0.14988301549827554
6	5	-0.1395539532179768
6	6	-0.10278666245944379
6	7	-0.14227198667084906
6	8	-0.12969130912157567
6	9	-0.19068827932415644
6	10	-0.24592973825514264
6	11	-0.16071183825845736
6	12	-0.20933853963843774
6	13	-0.22209332507588897
6	14	-0.17821164970612813
6	15	-0.2706174411303337
6	16	-0.27273594952176694
6	17	-0.1913507832496535
6	18	-0.2871054664525019
6	19	-0.337685834270367
6	20	-0.18176665857051688
6	21	-0.16491358525846067
6	22	-0.30517594567714923
6	23	-0.14577025941262178
6	24	-0.2769166247033965
6	25	-0.174447887009741
6	26	-0.23541986321085062
6	27	-0.08170995513975043
6	28	-0.15901834072974452
6	29	-0.1381000094797571
6	30	-0.1591369626358059
6	31	-0.024649582986441487
6	32	0.029223814085490574
6	33	-0.13460295097953434
6	34	-0.05904826956157194
6	35	0.05088486472230613
6	36	0.11177128359284438
6	37	0.19696256512883537
6	38	-0.09610136939705427
6	39	0.15221309969037122
6	40	-0.06786926344975086
6	41	0.07933370056420586
6	42	-0.03997090000358838
6	43	-0.054746362144534544
6	44	0.16620706748020808
6	45	0.047808996176463836
6	46	0.2447250864820134
6	47	0.211472231888294
6	48	0.10002665947068579
6	49	0.1610264071341021
6	50	0.1534901979340617
7	1	-0.0762854351090354
7	2	-0.1664381029573363
7	3	-0.15865203231691538
7	4	-0.14395615135590845
7	5	-0.10463711611424742
7	6	-0.1421839584514828
7	7	-0.24345164352173043
7	8	-0.2690942511869423
7	9	-0.15047448068663272
7	10	-0.13983163449405822
7	11	-0.11199519690804038
7	12	-0.11652166526921093
7	13	-0.12427125860673982
7	14	-0.1481673952742213
7	15	-0.16892977331495018
7	16	-0.22150632299172912
7	17	-0.1084065986388012
7	18	-0.16211742223318737
7	19	-0.11981956034601637
7	20	-0.12444120955803584
7	21	-0.12112591949559715
7	22	-0.043257822655824896
7	23	-0.08657851789376991
7	24	0.05678592398695059
7	25	0.008849399156311222
7	26	0.006376018071257761
7	27	-0.02820893960167581
7	28	-0.03694169424877458
7	29	-0.012963126566766903
7	30	0.08042531315263801
7	31	0.07363373814699514
7	32	0.049547566597183845
7	33	0.09327878694130476
7	34	0.12834826381669728
7	35	0.1227684501084525
7	36	0.26641192666259883
7	37	0.35493775234503033
7	38	0.10331113536266656
7	39	0.23633828159240977
7	40	0.15965302440906015
7	41	0.19018928754326225
7	42	0.255179715627726
7	43	0.3573365383942573
7	44	0.2652308221195483
7	45	0.10104313915304175
7	46	0.1050325973123023
7	47	0.19384200405013985
7	48	0.36417428893539494
7	49	0.06063882556257738
7	50	0.1277249620439207
8	1	-0.15166073404033398
8	2	-0.08507201937130304
8	3	-0.06790517320591505
8	4	-0.0790742603584107
8	5	-0.08997696936044264
8	6	-0.06971430607868001
8	7	-0.11011092135709788
8	8	-0.12227784462903912
8	9	-0.05715281471128009
8	10	-0.1241447283251702
8	11	-0.053585779669049316
8	12	-0.11555116527042486
8	13	-0.05895904551553599
8	14	-0.08432412162401448
8	15	-0.07664099886568465
8	16	-0.13212709652472654
8	17	-0.07017288787935172
8	18	-0.08520706280165058
8	19	-0.07602689238130012
8	20	-0.13477971169534442
8	21	-0.10476779524378799
8	22	-0.2421502084468088
8	23	-0.06492429291004953
8	24	-0.2911609899075458
8	25	-0.001507759756165683
8	26	0.04211107786256463
8	27	0.03265110176628383
8	28	0.017045810741002754
8	29	-0.04836319651789012
8	30	-0.12826973583720047
8	31	0.07706830369536988
8	32	-0.011303959613883308
8	33	-0.011765545382374398
8	34	-0.053881385857508235
8	35	0.1593497646570441
8	36	-0.09255430865287906
8	37	0.09386690309094092
8	38	0.08625008940204282
8	39	0.1493711898076313
8	40	0.10420837410215711
8	41	0.1168952523469926
8	42	-0.07212010707809213
8	43	0.022705116120398827
8	44	-0.2611754047340611
8	45	0.07660767041985395
8	46	0.08116247952256828
8	47	0.142023465395404
8	48	0.07125679457590195
8	49	0.2043262616062833
8	50	0.153471336511096
9	1	-0.03487414050022153
9	2	-0.10920792710550842
9	3	-0.08825719334691229
9	4	-0.08498085854667892
9	5	-0.0594041895118238
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113	9	-0.13520384879799963
113	10	-0.1299473088119356
113	11	-0.14077869273222632
113	12	-0.1509935666556146
113	13	-0.1655928659353023
113	14	-0.24408653656924173
113	15	-0.11852742448034803
113	16	-0.019207537771436023
113	17	-0.01766720162932245
113	18	-0.04765490557053198
113	19	-0.03646185335015417
113	20	-0.06332942562546862
113	21	-0.15641991739741457
113	22	-0.18463166919084983
113	23	-0.020305925940675706
113	24	0.019253902629294828
113	25	0.03000423659643303
113	26	-0.20978916308685314
113	27	0.027031357926439625
113	28	0.02801638259747108
113	29	0.014958063115159348
113	30	-0.0125159759060125
113	31	0.098952325528046
113	32	0.17332022912427192
113	33	0.14032591397558036
113	34	0.08606731403101264
113	35	0.1772989749120768
113	36	-0.050921107830646406
113	37	0.3973416970121022
113	38	0.18089738452363946
113	39	0.26003380478436355
113	40	0.08133276305832737
113	41	0.30233640054807864
113	42	0.23477502845756248
113	43	0.33811057247234544
113	44	0.3597103131521635
113	45	0.5398308669392904
113	46	0.5299479226292334
113	47	0.5198350888586916
113	48	0.12407735061893277
113	49	0.5675145671486561
113	50	0.5995204317180054
114	1	-0.08412867264952695
114	2	-0.12268959826676706
114	3	-0.13422440172411854
114	4	-0.08991639228584347
114	5	-0.08214966160225146
114	6	-0.10509983327794714
114	7	-0.11005384250848493
114	8	-0.17816227663947615
114	9	-0.08909514839529464
114	10	-0.052398237174415385
114	11	-0.023308902081289773
114	12	-2.493892356412175E-4
114	13	-0.08173253317796991
114	14	-0.154045078097802
114	15	-0.038040083913053024
114	16	-0.004091386106757966
114	17	-0.04345262022032159
114	18	-0.011642411102506856
114	19	0.001850108027909414
114	20	-0.06845147194657386
114	21	-0.0668042453239508
114	22	-0.051852176474085354
114	23	-0.018271994021205273
114	24	-0.04887645068894386
114	25	-0.09811535576022834
114	26	0.04142738259551493
114	27	0.05622221074182221
114	28	0.1000947478617249
114	29	0.10234796678680169
114	30	0.0277111673035364
114	31	0.174204133865409
114	32	0.05394529530908443
114	33	0.13250995457586612
114	34	0.18506693635676186
114	35	0.14870358645544712
114	36	0.2846293018877617
114	37	0.13934023650844551
114	38	0.2102760338699312
114	39	0.23997214043454562
114	40	0.3201546152781276
114	41	0.20697457086630777
114	42	0.1458798143921598
114	43	0.28174516988200793
114	44	0.33974793366972733
114	45	0.12639680414987708
114	46	0.08090854350855636
114	47	0.1772210076691323
114	48	0.33426463909533766
114	49	0.3182416053806705
114	50	0.2971906651721028
115	1	-0.1592546608865817
115	2	-0.13765105237742148
115	3	-0.12420445451346751
115	4	-0.11389580369582575
115	5	-0.13774818553992674
115	6	-0.05991421263279051
115	7	-0.047987679911003056
115	8	-0.09579603652094448
115	9	-0.03361924887997958
115	10	-0.10926643732373975
115	11	-0.03473632466815957
115	12	-0.0909964639193035
115	13	-0.0672355079444884
115	14	-0.09930179878310241
115	15	-0.1547372904498303
115	16	-0.10635209803784562
115	17	-0.12053489966861264
115	18	-0.15922243051223006
115	19	-0.015558567860402661
115	20	-0.045010126593851396
115	21	-0.0024625928570500832
115	22	-0.02588395998365911
115	23	-0.049446662157883736
115	24	0.05181541895593256
115	25	0.053684877295914646
115	26	-0.010337150136059847
115	27	0.020339332336121174
115	28	0.13545800557826482
115	29	0.11455141751356024
115	30	-0.0028255123450335873
115	31	0.09127828003665428
115	32	0.134155880329061
115	33	-0.0062849712885437725
115	34	0.189711682054174
115	35	0.2157502870251946
115	36	0.09415836662943633
115	37	0.21649011690814746
115	38	0.10198294105907024
115	39	0.3339231779624541
115	40	0.15853690118349562
115	41	0.2603898798725197
115	42	0.28596810445723975
115	43	0.15641002391650005
115	44	0.2723098642336197
115	45	0.2879419151593723
115	46	0.2942201351959213
115	47	0.3143674899847255
115	48	0.42798680495671704
115	49	0.41283500826370556
115	50	0.368779447454358
116	1	-0.14240087093178744
116	2	-0.22666154120434356
116	3	-0.15221022706585075
116	4	-0.17192183875414457
116	5	-0.15755499688401642
116	6	-0.13407428595198212
116	7	-0.12602121212999862
116	8	-0.11898686759686683
116	9	-0.07922823371436039
116	10	-0.13480871275746864
116	11	-0.11878219838817472
116	12	-0.17437659731427146
116	13	-0.17846475772477532
116	14	-0.1399144814931077
116	15	-0.20415501909575795
116	16	-0.11634863210817059
116	17	-0.16334399438316183
116	18	-0.1883132922750761
116	19	-0.14862375659716776
116	20	-0.15079452691359307
116	21	-0.22243866407328738
116	22	-0.1423330203187092
116	23	-0.06529934413634209
116	24	-0.16921098252151978
116	25	-0.10366486141226261
116	26	0.042304182458394735
116	27	0.015640254023814748
116	28	-0.09173618840368647
116	29	0.03893482850875074
116	30	0.05329258541752324
116	31	0.061422343918863476
116	32	0.07786927791190834
116	33	0.13160386959451387
116	34	0.14390862195880771
116	35	0.23781401699792326
116	36	0.19939591916873667
116	37	0.33087267034296985
116	38	0.2617109644083371
116	39	0.21011905506104256
116	40	0.24741774982246767
116	41	0.31531448135223883
116	42	0.14256969902537264
116	43	0.2630475235746417
116	44	0.35411238633737696
116	45	0.336436113773658
116	46	0.4428318147486294
116	47	0.5506761659474861
116	48	0.38745744736672094
116	49	0.5215457626400521
116	50	0.43136240368175294
117	1	-0.008905827787970111
117	2	-0.08759838611074144
117	3	-0.10943417621245999
117	4	-0.08604773965791424
117	5	-0.07103154806991085
117	6	-0.15097675993703064
117	7	-0.18012087739184324
117	8	-0.1337682572543386
117	9	-0.1393923107709938
117	10	-0.12775988198008292
117	11	-0.1361998789817136
117	12	-0.11903290638206698
117	13	-0.07442982790526997
117	14	-0.13473251025799016
117	15	-0.06804859653723128
117	16	-0.05194392600873954
117	17	-0.0761402175560093
117	18	-0.12245847354774497
117	19	-0.06614965010002294
117	20	-0.036325749211691516
117	21	-0.016917523512915977
117	22	0.004032038998751375
117	23	-0.013447413171761013
117	24	0.10935616641285861
117	25	0.07784202823715702
117	26	0.05008762404436595
117	27	0.08209949059416033
117	28	0.14426367949702978
117	29	0.055325439351769035
117	30	0.11688752551462755
117	31	0.00769992682947418
117	32	0.05451662081652131
117	33	0.08594517364759469
117	34	0.07283296419892338
117	35	0.1865986389030425
117	36	0.21411865795352725
117	37	0.23841896902153792
117	38	0.2143051643374747
117	39	0.23292277698200792
117	40	0.2223607364394411
117	41	0.24897817918967036
117	42	0.30739891966668864
117	43	0.27489360854207234
117	44	0.2574157964369519
117	45	0.20302858856038597
117	46	0.26104101626380327
117	47	0.43444609865634476
117	48	0.42760949182688535
117	49	0.4775451941644704
117	50	0.6351570051331663
118	1	-0.02304125040934224
118	2	-0.015934169725220215
118	3	0.02609518972224123
118	4	-0.03765018731358083
118	5	-0.022058661564869908
118	6	0.001186964879721586
118	7	-0.1166983917561808
118	8	-0.0890291331727795
118	9	0.0076522814373447545
118	10	-0.17169501335063586
118	11	-0.02655985402062555
118	12	-0.16097185538570358
118	13	-0.1067024678100097
118	14	-0.09740193167794331
118	15	-0.1450782170270415
118	16	-0.12779128712355714
118	17	-0.07536939373048313
118	18	-0.02700336906514167
118	19	-0.027077002155749597
118	20	0.002919096845445779
118	21	-0.0969708840931105
118	22	-0.0066541583957899775
118	23	0.04403542491490953
118	24	0.07968848724758004
118	25	0.001359494481427248
118	26	0.0777829392211018
118	27	0.051003635305448825
118	28	-0.010840530951604421
118	29	-0.04208079722044289
118	30	0.06633123705557153
118	31	0.2681436490551974
118	32	0.30164700525124033
118	33	0.2949995148238358
118	34	-0.017978215695187316
118	35	0.22051247282581699
118	36	0.4348162733444241
118	37	0.5161128757980009
118	38	0.36116925893131224
118	39	0.3716472471987444
118	40	0.335339866668825
118	41	0.3487230353015107
118	42	0.31566577394780637
118	43	0.4103157136766953
118	44	0.3706727865684343
118	45	0.3302479878107434
118	46	0.49343783418563447
118	47	0.5927128798700778
118	48	0.6084453474233342
118	49	0.6721103209812327
118	50	0.6345723310548337
119	1	-0.06567978868122282
119	2	-0.18842486253997492
119	3	-0.3216939396874139
119	4	-0.3031783774904824
119	5	-0.24385708968238617
119	6	-0.28804066865948386
119	7	-0.18644120651678264
119	8	-0.08441234170580714
119	9	-0.29961505038880887
119	10	-0.16645798145335533
119	11	-0.16839096426706135
119	12	-0.08102547637243163
119	13	-0.10612027169563021
119	14	-0.07631904015030955
119	15	-0.08017599100991646
119	16	-0.27384938625894506
119	17	-0.01481163971537569
119	18	-0.18509463059169207
119	19	0.009787430764980343
119	20	-0.08580104290012258
119	21	-0.12658819503704422
119	22	0.02148884989551192
119	23	0.014040758575504242
119	24	-0.20555113889091103
119	25	0.027600318152671832
119	26	-0.03899375922294013
119	27	-0.13150831249592443
119	28	0.09765318843806625
119	29	0.02133652616392112
119	30	0.11662691258947433
119	31	0.0560047957690486
119	32	0.07324332941922762
119	33	0.19218917093090937
119	34	0.12048093695144502
119	35	0.31247659879346656
119	36	0.25350005396305875
119	37	0.3048242409423665
119	38	0.37674395782003955
119	39	0.1938782968777275
119	40	0.3812591611428786
119	41	0.27399276491592417
119	42	0.4756135895181899
119	43	0.4905418802046384
119	44	0.493258631351857
119	45	0.45314120343947195
119	46	0.5452814887291453
119	47	0.3276618044114201
119	48	0.5269929424590529
119	49	0.2228293280253908
119	50	0.6787032789797252
120	1	-0.09694882799531257
120	2	-0.128055075377965
120	3	-0.12431385973108178
120	4	-0.1658852117901759
120	5	-0.1456332232077031
120	6	-0.130184455915888
120	7	-0.1032559610941135
120	8	-0.06386637491213776
120	9	-0.04860111347127827
120	10	-0.10109438707088714
120	11	-0.11155592296912431
120	12	-0.2578322228820582
120	13	-0.08058792653810798
120	14	-0.07281146202034705
120	15	-0.06829795188265564
120	16	-0.12137612479997983
120	17	-0.2456535327806364
120	18	-0.14603229480340474
120	19	-0.17282187629121637
120	20	-0.09019909177889929
120	21	-0.1561898602556795
120	22	-0.17356493449079835
120	23	-0.1582154970305396
120	24	-0.09132090188987974
120	25	-0.03090008509933284
120	26	0.01734492708825286
120	27	-0.07462914180053559
120	28	0.16533426985271404
120	29	0.03591455173052083
120	30	0.20370549985975117
120	31	0.1660951666238475
120	32	0.21489628270902017
120	33	0.2019546069639162
120	34	0.2846849980076982
120	35	0.35647831197501745
120	36	0.3484858264402817
120	37	0.3706898556754581
120	38	0.1857326305147211
120	39	0.2991898689129897
120	40	0.3326281571125307
120	41	0.37939930160893454
120	42	0.32199965938735886
120	43	0.4189717222230769
120	44	0.3560093668022901
120	45	0.5966144076658892
120	46	0.6395732228915918
120	47	0.4939243766418784
120	48	0.6644218358943661
120	49	0.5315934505865556
120	50	0.47079037332366624
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	10054.0
3	916.0
4	1123.0
5	1174.0
6	1801.0
7	2923.0
8	3595.0
9	4407.0
10	6618.0
11	9688.0
12	10987.0
13	15491.0
14	20667.0
15	23784.0
16	26999.0
17	32597.0
18	40430.0
19	50709.0
20	60610.0
21	63904.0
22	86031.0
23	127956.0
24	171808.0
25	144375.0
26	229225.0
27	271821.0
28	289022.0
29	284751.0
30	369938.0
31	455642.0
32	566787.0
33	649200.0
34	936092.0
35	1312142.0
36	1711784.0
37	2654674.0
38	4423254.0
39	1.3540244E7
40	49422.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.95325237813232	18.215101716130757	12.708073490145294	21.123572415591624
2	17.229953780457784	22.90511970447965	37.15765429499104	22.707272220071527
3	18.702694985686364	28.16867472990082	32.18802724546932	20.940603038943493
4	10.892847149760504	16.050556954899207	36.633106841085564	36.42348905425473
5	10.914198869319852	43.01106833717045	34.429887674765816	11.644845118743884
6	28.365375423532374	36.509038218148945	19.740959165192386	15.384627193126295
7	27.107389309133357	33.8342412139661	22.246283043835895	16.812086433064643
8	31.147929162539196	31.940762869954263	20.76150999536644	16.1497979721401
9	29.188689752062075	11.326335139139985	20.17042315549388	39.31455195330406
10	17.476613348226657	28.268692086357845	35.01709411013078	19.237600455284717
11	34.38756960496841	21.263264433551054	23.764450210369628	20.584715751110895
12	19.942158053406896	28.735114307534666	32.631918469335815	18.690809169722623
13	30.886570307799577	20.091174418829805	27.528481757353518	21.4937735160171
14	20.53808241667416	20.649192372582554	31.802329991161304	27.01039521958198
15	23.677111517889195	28.756284444010895	26.33145538343486	21.23514865466505
16	20.58537247739121	26.245158374984246	29.418236750635558	23.751232396988986
17	19.369614391429394	28.041077201085805	30.840650791981123	21.74865761550368
18	19.446136251556688	25.379123245604163	33.00768299645758	22.167057506381564
19	20.756199353255372	25.422966289865894	34.53545622318568	19.285378133693047
20	21.400926005782615	24.20600663111354	34.61693931601674	19.776128047087102
21	21.880798605820875	24.128542974868193	34.06404394353464	19.92661447577629
22	20.76487806198728	24.07967157379172	34.70891068355093	20.44653968067007
23	20.656813404946476	24.676815234560813	34.507437047345775	20.158934313146936
24	20.2194095529853	24.863883011716453	34.51395243178171	20.402755003516535
25	20.333819152413824	24.868583385824845	34.63266065691332	20.164936804848008
26	20.051616814759022	24.590241058321645	34.68625661211963	20.67188551479971
27	20.003234392494505	24.7092126547107	34.28279457793255	21.004758374862252
28	20.34776713125723	24.727185407136496	34.77668520764438	20.1483622539619
29	20.01745225972913	25.162640857376722	34.57542169751056	20.244485185383585
30	19.952659640650932	25.19500413320706	34.44580734700459	20.40652887913742
31	20.261610896483827	25.376363777074168	34.168355655823426	20.19366967061858
32	20.147724272596683	25.198298319582907	34.294003811761904	20.35997359605851
33	20.15214681924092	25.37752864431937	33.74162487648624	20.728699659953467
34	20.880215318490073	25.04289470591133	33.471851469590725	20.60503850600788
35	20.563817242888977	25.49729141697805	33.894870866867684	20.044020473265288
36	20.78887394400878	26.158443468628946	32.58763454755481	20.465048039807463
37	20.702991128923465	25.419058583850962	33.30963760726445	20.56831267996113
38	21.082006470469427	26.07104458821326	32.447094669150864	20.399854272166454
39	20.492562649411255	26.404273314249245	32.35519785916658	20.74796617717292
40	20.675394232998862	26.11089980694213	32.311768750672805	20.901937209386208
41	21.016705251555763	26.44121736296964	31.870408405763072	20.671668979711523
42	20.54922971117819	26.752255322466024	31.938791518475995	20.759723447879793
43	20.993361746823823	26.506123636221425	31.494019880364434	21.006494736590323
44	20.913377010902977	27.098881484737035	31.30426628964337	20.68347521471662
45	20.936427575225945	27.034572558979665	31.09701787316275	20.93198199263165
46	21.33691897564693	27.289540686680404	29.859896028388384	21.51364430928428
47	21.41849265689528	27.598821990831585	30.04652589307863	20.93615945919451
48	22.0461169398323	27.919567490373094	28.685067050380624	21.34924851941399
49	21.787472599936013	27.95663280631141	28.85272841070548	21.403166183047095
50	21.783442249356913	27.93297787167331	28.925825323569317	21.35775455540046
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	13170.0
1	11419.5
2	9669.0
3	27599.0
4	45529.0
5	55282.5
6	65036.0
7	55870.5
8	46705.0
9	60378.5
10	74052.0
11	105349.5
12	136647.0
13	245274.0
14	353901.0
15	373930.5
16	393960.0
17	503799.0
18	613638.0
19	576896.0
20	540154.0
21	540646.5
22	541139.0
23	552784.5
24	564430.0
25	585085.5
26	605741.0
27	648961.0
28	692181.0
29	742573.0
30	792965.0
31	878683.0
32	964401.0
33	1043706.5
34	1123012.0
35	1212134.5
36	1301257.0
37	1375630.5
38	1450004.0
39	1505484.5
40	1560965.0
41	1601281.0
42	1641597.0
43	1673638.5
44	1705680.0
45	1729863.0
46	1754046.0
47	1713823.0
48	1673600.0
49	1631551.0
50	1589502.0
51	1533171.0
52	1476840.0
53	1417813.0
54	1358786.0
55	1281313.5
56	1203841.0
57	1143222.0
58	1082603.0
59	1001212.0
60	919821.0
61	809129.0
62	698437.0
63	613212.5
64	527988.0
65	456596.5
66	385205.0
67	330525.5
68	275846.0
69	231958.5
70	188071.0
71	153789.5
72	119508.0
73	97756.5
74	76005.0
75	59435.0
76	42865.0
77	33561.0
78	24257.0
79	18880.0
80	13503.0
81	10589.5
82	7676.0
83	5936.5
84	4197.0
85	3131.0
86	2065.0
87	1654.5
88	1244.0
89	884.5
90	525.0
91	386.0
92	247.0
93	161.0
94	75.0
95	49.5
96	24.0
97	18.5
98	13.0
99	17.5
100	22.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.037501772777774
2	0.00938503756370007
3	4.116856626455793E-4
4	3.4888615478438927E-6
5	2.5817575454044805E-4
6	4.779740320546133E-4
7	0.003562127640348614
8	4.884406166981449E-5
9	3.7679704716714037E-4
10	4.919294782459888E-4
11	0.0
12	1.9188738513141408E-4
13	0.0
14	1.6397649274866295E-4
15	2.0933169287063355E-5
16	2.0933169287063355E-5
17	1.0466584643531676E-4
18	0.0
19	6.279950786119007E-5
20	6.593948325424957E-4
21	1.5350990810513126E-4
22	0.0013153008035371473
23	0.0030248429619806547
24	0.0031504419777030343
25	0.008568643961504599
26	0.033419804766796644
27	0.02830862259920534
28	0.025740820499992236
29	0.025573355145695732
30	0.0368423779452315
31	0.05521472285617744
32	0.04318163937766385
33	0.03830072207223025
34	0.056994042245577824
35	0.044636494643114755
36	0.052866719034478496
37	0.07099484363707537
38	0.07050989188192507
39	0.08661447678677248
40	0.08040430323161034
41	0.08243133179090764
42	0.10288303818436854
43	0.08666332084844229
44	0.09702523964553865
45	0.09586693761165446
46	0.11480796695489896
47	0.10086996507126261
48	0.12962865081013983
49	0.10928858798620993
50	0.12240670740610296
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2.8662645E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.5972220607951
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.03185717578533	25.285367932771123
2	12.33153347548224	8.779366709537394
3	5.417476416669515	5.785413330399157
4	2.979514445176802	4.242497493532213
5	1.8430961553869336	3.2804551561353192
6	1.2547158403356042	2.679863903497418
7	0.8855353991334953	2.2065820171954895
8	0.6376033538753598	1.815752653968712
9	0.5003001418200198	1.6028365720873072
>10	2.742071576009	17.4224658917526
>50	0.21104181711710043	5.209128790133993
>100	0.14421390787594218	9.936850550649186
>500	0.012882834150064028	3.1438730120505474
>1k	0.007587498922648084	4.86217166919164
>5k	3.2428887206741415E-4	0.7597313397653878
>10k+	2.4567338789634695E-4	2.9876429773327264
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	187431	0.6539208087739286	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	142362	0.49668130767415214	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	91061	0.31769922140821266	No Hit
TCTCTGAGCGGGCTGGCAAGGCAGACCGATATCGTATGCCGTCTTCTGCT	69505	0.24249332188288975	Illumina PCR Primer Index 1 (95% over 24bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	34495	0.12034827909287507	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	3.4888615478438923E-6	0.0
2	0.0	0.0	0.0	3.4888615478438923E-6	0.0
3	0.0	0.0	0.0	3.4888615478438923E-6	0.0
4	0.0	0.0	0.0	6.9777230956877846E-6	0.0
5	0.0	0.0	0.0	6.9777230956877846E-6	0.0
6	0.0	0.0	0.0	6.9777230956877846E-6	0.0
7	0.0	0.0	0.0	6.9777230956877846E-6	0.0
8	0.0	0.0	0.0	6.9777230956877846E-6	0.0
9	0.0	0.0	0.0	1.3955446191375569E-5	0.0
10	0.0	0.0	0.0	2.4422030834907247E-5	0.0
11	0.0	0.0	0.0	3.139975393059503E-5	0.0
12	0.0	0.0	0.0	3.488861547843892E-5	0.0
13	0.0	0.0	0.0	4.186633857412671E-5	0.0
14	0.0	0.0	0.0	5.233292321765838E-5	0.0
15	0.0	0.0	0.0	5.233292321765838E-5	0.0
16	0.0	0.0	0.0	5.931064631334617E-5	0.0
17	0.0	0.0	0.0	6.279950786119007E-5	0.0
18	0.0	0.0	0.0	6.977723095687784E-5	0.0
19	0.0	0.0	0.0	6.977723095687784E-5	0.0
20	0.0	0.0	0.0	8.024381560040953E-5	0.0
21	0.0	0.0	0.0	8.373267714825342E-5	0.0
22	0.0	0.0	0.0	1.0466584643531676E-4	0.0
23	0.0	0.0	0.0	1.2559901572238013E-4	0.0
24	0.0	0.0	0.0	1.5002104655728737E-4	0.0
25	0.0	0.0	0.0	1.709542158443507E-4	0.0
26	0.0	6.9777230956877846E-6	0.0	1.9886510822710187E-4	0.0
27	0.0	1.0466584643531678E-5	0.0	2.442203083490725E-4	0.0
28	0.0	1.0466584643531678E-5	0.0	4.1866338574126705E-4	0.0
29	0.0	1.0466584643531678E-5	0.0	6.524171094468078E-4	0.0
30	0.0	1.0466584643531678E-5	0.0	0.0010850359413794506	0.0
31	0.0	1.0466584643531678E-5	0.0	0.0015699876965297515	0.0
32	0.0	1.0466584643531678E-5	0.0	0.0023096263446726567	0.0
33	0.0	1.0466584643531678E-5	0.0	0.00362143828666196	0.0
34	0.0	1.0466584643531678E-5	0.0	0.005760110415490266	0.0
35	0.0	1.0466584643531678E-5	0.0	0.008914041254741144	0.0
36	0.0	1.0466584643531678E-5	0.0	0.014408998192595276	0.0
37	0.0	1.0466584643531678E-5	0.0	0.02434527588085468	0.0
38	0.0	1.0466584643531678E-5	0.0	0.03892871715084215	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	92065	0.0	37.84613	1
TATGCCG	14675	0.0	33.25229	35
GCCGTCT	14875	0.0	32.221302	38
CCGTCTT	15050	0.0	31.773886	39
GACCGAT	15615	0.0	31.405935	24
TCGTATG	15600	0.0	31.302803	32
CGTATGC	15705	0.0	31.125566	33
ATGCCGT	15390	0.0	31.027218	36
TATCGTA	10965	0.0	30.804695	30
ATATCGT	11020	0.0	30.6297	29
ACCGATA	11200	0.0	30.59117	25
ATCGTAT	11375	0.0	30.335348	31
AGACCGA	16900	0.0	30.305517	23
TGCCGTC	15910	0.0	30.096416	37
CGATATC	11390	0.0	29.965895	27
GATATCG	11655	0.0	29.285326	28
CGTCTTC	16465	0.0	29.09805	40
CCGATAT	11835	0.0	28.838865	26
GCGGGCT	18385	0.0	28.773565	8
CGGGCTG	18920	0.0	28.029663	9
>>END_MODULE