SRR522118_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR522118_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	32484511
Sequences flagged as poor quality	0
Sequence length	50
%GC	43

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	200346	0.6167431610714411	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	153671	0.47305929893788456	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	98546	0.303363039696057	No Hit
TCTCTGAGCGGGCTGGCAAGGCAGACCGATATCGTATGCCGTCTTCTGCT	71416	0.21984631383246003	Illumina PCR Primer Index 1 (95% over 24bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	40051	0.12329260551282425	No Hit
TCTCTGAGCGGGCTGGCAAGGCAGACCGATCTCGTATGCCGTCTTCTGCT	33684	0.10369249517100626	Illumina Paired End PCR Primer 2 (96% over 29bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	33528	0.1032122663013151	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	32849	0.10112203936208244	No Hit

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	100205	37.270103	1
TATGCCG	16280	32.777805	35
CCGTCTT	16870	30.983051	39
GCCGTCT	16815	30.963446	38
GACCGAT	17350	30.574108	24
TCGTATG	17475	30.512415	32
ATGCCGT	17250	30.399586	36
CGTATGC	17890	29.966734	33
ACCGATA	11935	29.845612	25
TATCGTA	11905	29.72307	30
ATATCGT	11885	29.693855	29
AGACCGA	18530	29.624372	23
ATCGTAT	12315	29.288578	31
TGCCGTC	17945	29.27121	37
GATATCG	12295	28.956865	28
CGATATC	12350	28.862875	27
CGTCTTC	18200	28.65268	40
CCGATAT	12510	28.495173	26
TGAGCGG	20945	27.44912	5
GCGGGCT	20740	27.435276	8