FastQCFastQC Report
Thu 26 May 2016
SRR522117_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR522117_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27492851
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCG2059970.7492747841975356Illumina Paired End PCR Primer 2 (100% over 31bp)
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT692950.25204734132520484No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA263200.036.42035721
AGATCGG264900.036.13722220
TGCCGAG271400.035.36337715
ATGCCGA272200.035.2592514
ATCGGAA273650.035.00862522
CGAGATC275300.034.80662518
TCGGAAG275300.034.774223
GAGCGGT274950.034.48366529
CCGAGAT278250.034.42826517
AAGAGCG277250.034.38905727
GAGATCG279650.034.2326319
AGAGCGG284050.033.6206928
GCGGTTC282100.033.60742631
AGCGGTT282600.033.53907430
GCCGAGA294850.032.52925516
CGGAAGA301550.031.8062124
GAAGAGC380450.025.32610326
GGAAGAG438300.022.10849625
CAACGCA215500.020.66082213
TCAACGC218150.020.5106312
Produced by FastQC (version 0.11.5)