##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR522117_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	27492851
Sequences flagged as poor quality	0
Sequence length	50
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	39.320668125688385	40.0	39.0	40.0	38.0	40.0
2	39.251595041925626	40.0	39.0	40.0	38.0	40.0
3	39.245810301739894	40.0	39.0	40.0	38.0	40.0
4	39.24455888550809	40.0	39.0	40.0	38.0	40.0
5	39.24242283930466	40.0	39.0	40.0	38.0	40.0
6	39.27373778005053	40.0	39.0	40.0	38.0	40.0
7	39.24520523535373	40.0	39.0	40.0	38.0	40.0
8	39.23352376223186	40.0	39.0	40.0	38.0	40.0
9	39.22170690846141	40.0	39.0	40.0	38.0	40.0
10	39.20902091965653	40.0	39.0	40.0	38.0	40.0
11	39.218042792288074	40.0	39.0	40.0	38.0	40.0
12	39.19078279658956	40.0	39.0	40.0	38.0	40.0
13	39.16348748261866	40.0	39.0	40.0	38.0	40.0
14	39.15193415190007	40.0	39.0	40.0	38.0	40.0
15	39.13418364650505	40.0	39.0	40.0	38.0	40.0
16	39.11925365615956	40.0	39.0	40.0	38.0	40.0
17	39.08855076543353	40.0	39.0	40.0	38.0	40.0
18	39.06119870943905	40.0	39.0	40.0	38.0	40.0
19	39.02936901669456	40.0	39.0	40.0	38.0	40.0
20	38.99306728865624	40.0	39.0	40.0	38.0	40.0
21	38.96482416465284	40.0	39.0	40.0	38.0	40.0
22	38.918506196392656	40.0	39.0	40.0	37.0	40.0
23	38.89183453545796	40.0	39.0	40.0	37.0	40.0
24	38.8596942528805	40.0	39.0	40.0	37.0	40.0
25	38.82219388596694	40.0	39.0	40.0	37.0	40.0
26	38.71659654358873	40.0	39.0	40.0	36.0	40.0
27	38.638881904244855	40.0	39.0	40.0	36.0	40.0
28	38.597272141765146	40.0	39.0	40.0	36.0	40.0
29	38.555025995667016	40.0	39.0	40.0	36.0	40.0
30	38.472005940744374	40.0	39.0	40.0	36.0	40.0
31	38.41927488713338	40.0	39.0	40.0	36.0	40.0
32	38.322374132824564	40.0	38.0	40.0	35.0	40.0
33	38.55235802936552	40.0	39.0	40.0	36.0	40.0
34	38.520097497345766	40.0	39.0	40.0	36.0	40.0
35	38.45128320813291	40.0	39.0	40.0	36.0	40.0
36	38.523900704223074	40.0	39.0	40.0	36.0	40.0
37	38.47513795495418	40.0	39.0	40.0	36.0	40.0
38	38.41923698637147	40.0	39.0	40.0	36.0	40.0
39	38.39325270413025	40.0	39.0	40.0	36.0	40.0
40	38.341408426503314	40.0	39.0	40.0	36.0	40.0
41	38.27655505789487	40.0	39.0	40.0	36.0	40.0
42	38.21225856132563	40.0	39.0	40.0	35.0	40.0
43	38.15169910897928	40.0	39.0	40.0	35.0	40.0
44	38.087875135248794	40.0	39.0	40.0	35.0	40.0
45	38.047253084083565	40.0	38.0	40.0	35.0	40.0
46	37.94564125052	40.0	38.0	40.0	35.0	40.0
47	37.8910982349557	40.0	38.0	40.0	35.0	40.0
48	37.884501065386054	40.0	38.0	40.0	35.0	40.0
49	37.8386986857056	40.0	38.0	40.0	35.0	40.0
50	37.782049195261706	40.0	38.0	40.0	35.0	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1	1	-0.11507125965827214
1	2	-0.14154066427009582
1	3	-0.12959994457914092
1	4	-0.14011724076630117
1	5	-0.11135683899527038
1	6	-0.12874212469362334
1	7	-0.12844158930378313
1	8	-0.13323570068763502
1	9	-0.1434707776837456
1	10	-0.1367052623783067
1	11	-0.1314212061495823
1	12	-0.14122349769830578
1	13	-0.1345620914324286
1	14	-0.14957764904711013
1	15	-0.14315221432671876
1	16	-0.15210986542997063
1	17	-0.1534702157374852
1	18	-0.16997213103966402
1	19	-0.18794719958410155
1	20	-0.19095113841724043
1	21	-0.20007779364575384
1	22	-0.2003262155234964
1	23	-0.170849248663842
1	24	-0.2177926281243714
1	25	-0.15676317029901554
1	26	-0.16740203436643242
1	27	-0.1688564316417711
1	28	-0.15738992288429898
1	29	-0.17495946338154766
1	30	-0.20483996283790162
1	31	-0.1541116105784397
1	32	-0.16088841738341841
1	33	-0.12912969571769395
1	34	-0.13270619327877142
1	35	-0.1329086910215196
1	36	-0.13032311076123904
1	37	-0.14572193378234743
1	38	-0.1533514674644323
1	39	-0.15238708020176261
1	40	-0.13281264105162904
1	41	-0.1599802442008098
1	42	-0.17356025494083127
1	43	-0.17523651091869397
1	44	-0.12657385809727373
1	45	-0.19726152986302736
1	46	-0.10586218876260034
1	47	-0.10342923306846785
1	48	-0.0641418280593058
1	49	-0.04651834438126912
1	50	-0.052760062482391845
2	1	-0.12630448990584853
2	2	-0.15061599198899955
2	3	-0.14052808228387903
2	4	-0.14663963606657404
2	5	-0.1258808312038795
2	6	-0.13978424940680867
2	7	-0.15426236211083477
2	8	-0.17816416613325714
2	9	-0.18681469507735216
2	10	-0.15304671508741308
2	11	-0.17704217198966177
2	12	-0.17824118684642087
2	13	-0.17578371548658112
2	14	-0.19549890290826255
2	15	-0.19711853438564475
2	16	-0.20135348937848363
2	17	-0.19972532569874346
2	18	-0.21805384388954963
2	19	-0.19763502402373945
2	20	-0.2642512801519814
2	21	-0.20143180310505926
2	22	-0.17048808746365296
2	23	-0.20651991871540787
2	24	-0.2180779872302523
2	25	-0.2112336608032237
2	26	-0.21291066339979636
2	27	-0.21680354484040265
2	28	-0.23996999030045174
2	29	-0.21294356341731913
2	30	-0.20464438282208874
2	31	-0.21866663266645503
2	32	-0.2219452457998372
2	33	-0.2338520198564069
2	34	-0.2161797069660807
2	35	-0.2806994449006268
2	36	-0.19434099083228773
2	37	-0.20818229123633358
2	38	-0.24007405252438474
2	39	-0.2137392987864004
2	40	-0.2135015651072507
2	41	-0.19329081466946718
2	42	-0.18645474733167333
2	43	-0.220591032752111
2	44	-0.18921701056150653
2	45	-0.22933388769223484
2	46	-0.20210941495270873
2	47	-0.22001223608528875
2	48	-0.21055139149235202
2	49	-0.19614527367168222
2	50	-0.27303061981976384
3	1	-0.12581066990379952
3	2	-0.13870193557747257
3	3	-0.10948200464876123
3	4	-0.1147908027276543
3	5	-0.12498508112680895
3	6	-0.12215843882955113
3	7	-0.12694701551282606
3	8	-0.11492024462570072
3	9	-0.11802556437456246
3	10	-0.13691976782789794
3	11	-0.0970233218534915
3	12	-0.11157703003383546
3	13	-0.1252401360398281
3	14	-0.11124014993022513
3	15	-0.13241655884885262
3	16	-0.12024873113417556
3	17	-0.139834041864205
3	18	-0.15437247055482572
3	19	-0.14058370220177352
3	20	-0.11625487678116286
3	21	-0.12898025136500024
3	22	-0.11765502685950935
3	23	-0.13523376207925253
3	24	-0.1573416639676708
3	25	-0.12501551251502718
3	26	-0.15795326948427402
3	27	-0.12509661351528933
3	28	-0.15632785241834313
3	29	-0.12724924845769436
3	30	-0.1416100934938953
3	31	-0.16986416853645636
3	32	-0.1736835820537408
3	33	-0.1808262195781154
3	34	-0.17077630866783977
3	35	-0.17474791269545875
3	36	-0.16251119814651105
3	37	-0.15775040167653742
3	38	-0.190737790199627
3	39	-0.1914992802537938
3	40	-0.2014948484174539
3	41	-0.16521548204822523
3	42	-0.14028603405709816
3	43	-0.15516378669847342
3	44	-0.14997584243841544
3	45	-0.2003156617062416
3	46	-0.16328059459378608
3	47	-0.16270298464996102
3	48	-0.15597631268666134
3	49	-0.13546995173595633
3	50	-0.16072902150658308
4	1	-0.10826736947953464
4	2	-0.13301155964625622
4	3	-0.11631500536816475
4	4	-0.11209500373485781
4	5	-0.1084249338664236
4	6	-0.11190395741695625
4	7	-0.13027332304260142
4	8	-0.11288009980635394
4	9	-0.10821785757433133
4	10	-0.13678039420477717
4	11	-0.10608164830563283
4	12	-0.11286156908823841
4	13	-0.12066209908504533
4	14	-0.11276232324141944
4	15	-0.11778754431097127
4	16	-0.1395298061983823
4	17	-0.11403434963198578
4	18	-0.12099136471854877
4	19	-0.11530609530046831
4	20	-0.11806873611039492
4	21	-0.12013412750050634
4	22	-0.16464448172684598
4	23	-0.11359928594951185
4	24	-0.11027387821334145
4	25	-0.10482723437262109
4	26	-0.12341895565114669
4	27	-0.1250412830197689
4	28	-0.12656121519790275
4	29	-0.12610457937721264
4	30	-0.12127187534515826
4	31	-0.09064000152204699
4	32	-0.10278061848442377
4	33	-0.1014846193286516
4	34	-0.10004306342246849
4	35	-0.10888362486488745
4	36	-0.11248455588616935
4	37	-0.1371318126255403
4	38	-0.11595961400077925
4	39	-0.1504453144874276
4	40	-0.13697087601126157
4	41	-0.09757098579036239
4	42	-0.1031137923697969
4	43	-0.13568125209705784
4	44	-0.10731417959357259
4	45	-0.10302408146981179
4	46	-0.10189537641538493
4	47	-0.13064105305890905
4	48	-0.10716806022023206
4	49	-0.10238219763736822
4	50	-0.10533581753169585
5	1	-0.08925712944989783
5	2	-0.11959071910901997
5	3	-0.1044184422708554
5	4	-0.1086376425201081
5	5	-0.10006320260528412
5	6	-0.1119929285618042
5	7	-0.11157641963905007
5	8	-0.10391413013400097
5	9	-0.11450957713145726
5	10	-0.12010731262467544
5	11	-0.13326330044453982
5	12	-0.1362209645082828
5	13	-0.125963857455325
5	14	-0.127104343754894
5	15	-0.11806802392456461
5	16	-0.13001479322959142
5	17	-0.1288217835398271
5	18	-0.11222535107903298
5	19	-0.13657625362392878
5	20	-0.11115489571016468
5	21	-0.12703664947367344
5	22	-0.09872663115498881
5	23	-0.1264526715408607
5	24	-0.10719944004264903
5	25	-0.12448817583858585
5	26	-0.15043001195049754
5	27	-0.14625337252022064
5	28	-0.11998987813964845
5	29	-0.15606479154951813
5	30	-0.1391157540268253
5	31	-0.11580796379296032
5	32	-0.17029456074936178
5	33	-0.14550325400062292
5	34	-0.13256529460004174
5	35	-0.1726322240601661
5	36	-0.15408111260783386
5	37	-0.16329290000760466
5	38	-0.14689196183314834
5	39	-0.15001351564144727
5	40	-0.12722856410263716
5	41	-0.10627285314076573
5	42	-0.06978709360363666
5	43	-0.08276585859694308
5	44	-0.06545981141024981
5	45	-0.07987252344081242
5	46	-0.11285904277360714
5	47	-0.08770712374239054
5	48	-0.07108890664145662
5	49	-0.07979281375972391
5	50	-0.0854225730190521
6	1	-0.053901699759038024
6	2	-0.08308963233751854
6	3	-0.0805949022201915
6	4	-0.06860101683211184
6	5	-0.06459403552683085
6	6	-0.07085761965411308
6	7	-0.06226124425586477
6	8	-0.06651816634465746
6	9	-0.0831768467617735
6	10	-0.06581089181779731
6	11	-0.09408983736240373
6	12	-0.0909867444284913
6	13	-0.08873480248262666
6	14	-0.09302458402346758
6	15	-0.0857525462528983
6	16	-0.11536137437951055
6	17	-0.1132668790553808
6	18	-0.11402621355356501
6	19	-0.09006769659736591
6	20	-0.0975378196657104
6	21	-0.09163373790075724
6	22	-0.07695917846454847
6	23	-0.11024066707666691
6	24	-0.09428952504410404
6	25	-0.08689864153201654
6	26	-0.10970654027165949
6	27	-0.11429445185417819
6	28	-0.0673477738084074
6	29	-0.07961835933923567
6	30	-0.09403878602048366
6	31	-0.05397032707405458
6	32	-0.05587385291031666
6	33	-0.08042547310260773
6	34	-0.10783393674303454
6	35	-0.07698469392450136
6	36	-0.1481863829884773
6	37	-0.11523563555522287
6	38	-0.13458816088923697
6	39	-0.10091422607322187
6	40	-0.09345385150971453
6	41	-0.0841492689666552
6	42	-0.060529505930567495
6	43	-0.09230519825240435
6	44	-0.12222056302721995
6	45	-0.07549866863763555
6	46	-0.062330456793254996
6	47	-0.02413731016118703
6	48	-0.05056356701732767
6	49	-0.02697801843422809
6	50	-0.023342552335556377
7	1	-0.07916224329454025
7	2	-0.07940076319950151
7	3	-0.07484994044496318
7	4	-0.08070382913638952
7	5	-0.07976157150786634
7	6	-0.0667994506648455
7	7	-0.08847158224125451
7	8	-0.08382697675595807
7	9	-0.06188280835041837
7	10	-0.08052898449236778
7	11	-0.07247184561354914
7	12	-0.07842890834297123
7	13	-0.07687842677302825
7	14	-0.07864815400194658
7	15	-0.08271508558200225
7	16	-0.0962308998871606
7	17	-0.07802650347063178
7	18	-0.08364930865544551
7	19	-0.10270704031962197
7	20	-0.08189407678653282
7	21	-0.1290927632315615
7	22	-0.0966026264624773
7	23	-0.11946379381753758
7	24	-0.09470767320123485
7	25	-0.0783234465105167
7	26	-0.07980501138789009
7	27	-0.08216844967187598
7	28	-0.08740188611661637
7	29	-0.07235266073261926
7	30	-0.057451001265214074
7	31	-0.08287342828899824
7	32	-0.1184039743810672
7	33	-0.11134953707051665
7	34	-0.1111075024696433
7	35	-0.12390962022013241
7	36	-0.0993387577329301
7	37	-0.09669424293380757
7	38	-0.11578899541299847
7	39	-0.11392119462038863
7	40	-0.10696554954535031
7	41	-0.12918912620396839
7	42	-0.12986826479358626
7	43	-0.1641730966406385
7	44	-0.1586373507211718
7	45	-0.1179812924600796
7	46	-0.11530961778157689
7	47	-0.10212625867320924
7	48	-0.09693736986682211
7	49	-0.07947058353069991
7	50	-0.04978185625818554
8	1	-0.06871808650047484
8	2	-0.07814732173088856
8	3	-0.05726459478125179
8	4	-0.05885454176522842
8	5	-0.06252180636725058
8	6	-0.06534244307650283
8	7	-0.07902781698996364
8	8	-0.06957344018761091
8	9	-0.06818388466276559
8	10	-0.08125306657078823
8	11	-0.0793660409046808
8	12	-0.08942907872532402
8	13	-0.0830553994671348
8	14	-0.07805672488886017
8	15	-0.08011850540891885
8	16	-0.09361572372721128
8	17	-0.09628321994228628
8	18	-0.10434955601401441
8	19	-0.12108230266213127
8	20	-0.10059234979378573
8	21	-0.08048740514659158
8	22	-0.07514477588193813
8	23	-0.09648047817367456
8	24	-0.09516395187077364
8	25	-0.07200952154609297
8	26	-0.09373642850158603
8	27	-0.07966603024919294
8	28	-0.10570537842937
8	29	-0.07618658834410041
8	30	-0.10320131332123594
8	31	-0.06236969350291588
8	32	-0.09192110210676674
8	33	-0.1161530579743939
8	34	-0.12497081590532844
8	35	-0.12963674678121606
8	36	-0.1196929274612657
8	37	-0.14233509587919002
8	38	-0.14121938439194537
8	39	-0.12621055190810182
8	40	-0.12015354069382767
8	41	-0.11985098054511667
8	42	-0.13291879633882786
8	43	-0.1061476881512462
8	44	-0.11933119674304038
8	45	-0.11188295226275358
8	46	-0.13612517757538
8	47	-0.16068267163407768
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112	38	0.01859832483547308
112	39	-0.010536564954513494
112	40	0.003312195342026314
112	41	0.022449799429033135
112	42	0.05039439671987367
112	43	0.03871451113545277
112	44	0.0050493443935195614
112	45	1.5959505252993722E-4
112	46	0.07036641546879707
112	47	0.08102554277664353
112	48	0.09941089798125802
112	49	0.10535164093825955
112	50	0.12405482165530657
113	1	-0.039635958110601166
113	2	-0.049343066490642684
113	3	-0.05044061833639546
113	4	-0.04915916166175549
113	5	-0.04102751128471738
113	6	-0.04253689956907891
113	7	-0.03931265358936287
113	8	-0.06458011330303037
113	9	-0.035795688242302504
113	10	-0.06026916676932359
113	11	-0.05992364418487739
113	12	-0.05929565636832024
113	13	-0.07357689942709555
113	14	-0.064812429760579
113	15	-0.07131150266641839
113	16	-0.05203532834671876
113	17	-0.07177232810408896
113	18	-0.04809082120449659
113	19	-0.07639086189561795
113	20	-0.051039528735081774
113	21	-0.03484421361820722
113	22	-0.06250460797841839
113	23	-0.054976946677186334
113	24	-0.040591844621026496
113	25	-0.024173262677400942
113	26	-0.03519632750305135
113	27	-0.03594807414933143
113	28	-0.05667200120227989
113	29	-0.051575201784714864
113	30	-0.02237564177514173
113	31	-0.015158582126815645
113	32	0.009756765101990084
113	33	-0.06620547521827547
113	34	-0.08163236522578643
113	35	-0.0338543045557671
113	36	-0.0703525963788536
113	37	-0.07770742619843674
113	38	-0.08753606011732984
113	39	-0.07832123230237897
113	40	-0.059643440321700325
113	41	-0.061759822756997096
113	42	-0.04578058053733258
113	43	-0.05651854148598545
113	44	-0.040840080211680174
113	45	-0.05685588620906401
113	46	-0.03224281873566781
113	47	-0.04791204693154327
113	48	-0.0018216722170194544
113	49	0.005494660779874039
113	50	0.005867494060460388
114	1	-0.052869954210265746
114	2	-0.06132416691416864
114	3	-0.05814711063590039
114	4	-0.0399143885798523
114	5	-0.047488954914825854
114	6	-0.07270344351808689
114	7	-0.05835696699069359
114	8	-0.04838091242682907
114	9	-0.059910512986377285
114	10	-0.04669098604346544
114	11	-0.051896525187778764
114	12	-0.05558361944916612
114	13	-0.06989939241188381
114	14	-0.06595812810003565
114	15	-0.05000911892376081
114	16	-0.03305324839541157
114	17	-0.03860751127741935
114	18	-0.05944686642229158
114	19	-0.01883574043145586
114	20	-0.038066964772767165
114	21	-0.031108752500053072
114	22	-0.03515251097476835
114	23	-0.024444194220954785
114	24	-0.019930508299232486
114	25	-0.03576275211895563
114	26	-0.05090218452856732
114	27	-0.02313251641827918
114	28	-0.054993127575123424
114	29	-0.06479674873498453
114	30	-0.02578382968107462
114	31	-0.030190340538688076
114	32	-0.028106704100487434
114	33	-0.043312285704452336
114	34	-0.06854003641900164
114	35	-0.029170737493231513
114	36	-0.04560515993831871
114	37	-0.07790794066202977
114	38	-0.02848273985863159
114	39	-0.029852807852094543
114	40	-0.03079665069832771
114	41	-0.017031730563822123
114	42	0.005296461027562316
114	43	-0.015432047605827393
114	44	-0.0021671841929347124
114	45	0.01887214970085438
114	46	-0.005095787613242919
114	47	0.028967142563075754
114	48	0.053219727883522694
114	49	0.05485992355139757
114	50	0.0649481197506816
115	1	-0.05966441969086844
115	2	-0.05993752784074502
115	3	-0.05674334717468099
115	4	-0.05337885897115768
115	5	-0.05801179005872825
115	6	-0.05476639919041304
115	7	-0.053386460185500084
115	8	-0.053372765078847806
115	9	-0.07465543222301818
115	10	-0.057031437851343014
115	11	-0.06064247334013828
115	12	-0.05119172429840546
115	13	-0.0592062721262252
115	14	-0.07297311499070247
115	15	-0.0640775402924163
115	16	-0.06800875300185538
115	17	-0.0640875691132834
115	18	-0.04532707340006681
115	19	-0.07314981284916655
115	20	-0.049946666833243114
115	21	-0.05065682698199936
115	22	-0.04781651663959963
115	23	-0.06766715909333954
115	24	-0.044517434645655385
115	25	-0.03621716742213721
115	26	-0.04718617878800302
115	27	-0.005971059499344733
115	28	-0.048357147986529014
115	29	-0.054319158391749056
115	30	-0.023023184205072766
115	31	0.0030304886468499603
115	32	0.0010694706062039927
115	33	-0.03328836028344995
115	34	-0.01779265299171584
115	35	-0.012219877932075462
115	36	-0.01127881880717041
115	37	-0.011146283316193717
115	38	-0.0416282047372718
115	39	-0.025144784907162432
115	40	-0.04770805713150139
115	41	-3.713363722823715E-4
115	42	-0.023050459689166303
115	43	0.01371508116783815
115	44	-0.015502658512595247
115	45	0.012038422839516727
115	46	-0.007637438108027084
115	47	0.029045841973584174
115	48	0.024401530184633202
115	49	0.03894795207231283
115	50	0.06262377927706808
116	1	-0.06784232351538577
116	2	-0.08778736238281226
116	3	-0.08585181055900648
116	4	-0.07944871440863466
116	5	-0.06722912439118289
116	6	-0.08946205544284425
116	7	-0.08397236805318187
116	8	-0.09089366515399178
116	9	-0.09375474370015269
116	10	-0.08828140779056781
116	11	-0.0860338300885104
116	12	-0.07475598302630715
116	13	-0.08557943799491596
116	14	-0.11379839674918202
116	15	-0.0978032881411508
116	16	-0.11380013738872208
116	17	-0.07655691125462027
116	18	-0.09292022509040265
116	19	-0.09423794671224073
116	20	-0.09863796042472472
116	21	-0.08694445722437649
116	22	-0.10583498049420825
116	23	-0.1100021179317352
116	24	-0.08246216224387126
116	25	-0.08330765827633968
116	26	-0.08390077850940969
116	27	-0.11729990766224319
116	28	-0.08244478513167763
116	29	-0.09605248329213367
116	30	-0.07629474823283999
116	31	-0.08519124644612219
116	32	-0.08583387742901039
116	33	-0.08558212676879862
116	34	-0.08930933043886569
116	35	-0.0742991352215796
116	36	-0.07410772147481737
116	37	-0.08875728021447316
116	38	-0.07378902175351243
116	39	-0.06901516252818851
116	40	-0.056150311133301045
116	41	-0.043436858045872384
116	42	-0.02342502551217507
116	43	-0.062019861271039645
116	44	-0.02060314155511378
116	45	-0.05722123724331141
116	46	-0.05144589686042167
116	47	-0.02858629978203453
116	48	-0.018930423398202834
116	49	0.0035251161713887313
116	50	-0.020100057038412444
117	1	-0.0682313121489031
117	2	-0.09122014582425209
117	3	-0.07800258485979583
117	4	-0.08410217303603673
117	5	-0.07746604842687788
117	6	-0.058360053145477764
117	7	-0.06274495469180863
117	8	-0.06350755330242208
117	9	-0.07970780878974892
117	10	-0.08195576836961749
117	11	-0.08384916365498896
117	12	-0.07380910364985027
117	13	-0.08784970048267837
117	14	-0.07628875985333394
117	15	-0.09905932472911161
117	16	-0.09011982971149735
117	17	-0.07348979221919194
117	18	-0.07920723809916552
117	19	-0.09540355476078588
117	20	-0.09022697593930928
117	21	-0.06624737094430344
117	22	-0.057212307028649434
117	23	-0.08114293336428346
117	24	-0.032671013827105355
117	25	-0.0603697785667876
117	26	-0.07453330399126656
117	27	-0.07893329587188447
117	28	-0.07254153450885781
117	29	-0.051555665884372104
117	30	-0.06800920725307691
117	31	-0.06877812010697681
117	32	-0.04174448167916722
117	33	-0.09729217049471828
117	34	-0.09421552128835486
117	35	-0.05720887231075267
117	36	-0.051471452255547945
117	37	-0.06526689272769914
117	38	-0.04824186574810341
117	39	-0.06046482279288057
117	40	-0.0713681122823786
117	41	-0.06366783703852263
117	42	-0.07535432402278275
117	43	-0.07644547541539026
117	44	-0.0657571593312909
117	45	-0.03907140938738962
117	46	-0.020975037384587836
117	47	-0.018500183927372404
117	48	0.019585789919851493
117	49	0.011388545736501499
117	50	0.029059447986512055
118	1	-0.09592871448117535
118	2	-0.12101164076752724
118	3	-0.10271869660525823
118	4	-0.10571410319008123
118	5	-0.11458713584852376
118	6	-0.1065477610398986
118	7	-0.1312306088564057
118	8	-0.12181960909939704
118	9	-0.11630038640938523
118	10	-0.11377127737973325
118	11	-0.10921961640414679
118	12	-0.1211989721880542
118	13	-0.12747601182157808
118	14	-0.09845264059551084
118	15	-0.13364675286334204
118	16	-0.10197805713059438
118	17	-0.1172567277020562
118	18	-0.11399027012950569
118	19	-0.10986905171039041
118	20	-0.09955773897101494
118	21	-0.09421396289009465
118	22	-0.10531578217208448
118	23	-0.09655943402871969
118	24	-0.0937366493050078
118	25	-0.08369130636445732
118	26	-0.11982824315973062
118	27	-0.10001098586926105
118	28	-0.1282910714981469
118	29	-0.10190084220793949
118	30	-0.13012779684169828
118	31	-0.13792959185414588
118	32	-0.14414040831456276
118	33	-0.1596165060723962
118	34	-0.14032580394018623
118	35	-0.13083323211186837
118	36	-0.11600565755688308
118	37	-0.14375635104276796
118	38	-0.10981303334458659
118	39	-0.11593944991280836
118	40	-0.11919654771457289
118	41	-0.09169517478319023
118	42	-0.10693443981620021
118	43	-0.09658956916502603
118	44	-0.08259850142795955
118	45	-0.07002060476559535
118	46	-0.08861259040398295
118	47	-0.0646513653264762
118	48	-0.0381807222640802
118	49	-0.03698749058107609
118	50	-0.049307876734872025
119	1	-0.0778755254993797
119	2	-0.09177355105378382
119	3	-0.07940414828059517
119	4	-0.08808449624020653
119	5	-0.09791561041578234
119	6	-0.0766845931775606
119	7	-0.08119441036252795
119	8	-0.09384246479968539
119	9	-0.10458225825291834
119	10	-0.07800835580521692
119	11	-0.10634393387108076
119	12	-0.11281732594441962
119	13	-0.1006239236689197
119	14	-0.11139431104341213
119	15	-0.1085882844793673
119	16	-0.11697323771520018
119	17	-0.11651805042035335
119	18	-0.10336888945152367
119	19	-0.11504568358547829
119	20	-0.12120830426819396
119	21	-0.14126817740382336
119	22	-0.13773774054968868
119	23	-0.14094571606996453
119	24	-0.15295799253107845
119	25	-0.12451979771171295
119	26	-0.1082644470232097
119	27	-0.0805740430179327
119	28	-0.10378382941762254
119	29	-0.11081446366918613
119	30	-0.08059928528938087
119	31	-0.09167253254300789
119	32	-0.11210568393340736
119	33	-0.11381904215046035
119	34	-0.10131230638488375
119	35	-0.08980091608196972
119	36	-0.10245378512175307
119	37	-0.08189689573708847
119	38	-0.08265480683777326
119	39	-0.08383578040895401
119	40	-0.10158329603343219
119	41	-0.10129864171103264
119	42	-0.08185261628431562
119	43	-0.11905734624728126
119	44	-0.06371924161629039
119	45	-0.07918074790342189
119	46	-0.08680661023277736
119	47	-0.11300363907874811
119	48	-0.03754487059804745
119	49	-0.06633921488112549
119	50	-0.05275244268152335
120	1	-0.10819347066428975
120	2	-0.14671745915499912
120	3	-0.14132427936353764
120	4	-0.13293416508281553
120	5	-0.16184522065651663
120	6	-0.14460166887856474
120	7	-0.1517892356672519
120	8	-0.1460179820375771
120	9	-0.16915175842720487
120	10	-0.1598628421172421
120	11	-0.15936736387908468
120	12	-0.15195225398459655
120	13	-0.15154122522935864
120	14	-0.1600535098804201
120	15	-0.1630460490556942
120	16	-0.16401768608762524
120	17	-0.14574113555381984
120	18	-0.15478580746812298
120	19	-0.1695410184882391
120	20	-0.20731134008848073
120	21	-0.16028138328051256
120	22	-0.16648032076263064
120	23	-0.14881245244996677
120	24	-0.1615615611751764
120	25	-0.16225904895513565
120	26	-0.14473001948055497
120	27	-0.14836771244598168
120	28	-0.14991733208945135
120	29	-0.16858042242606075
120	30	-0.16817359548068112
120	31	-0.13376418916526234
120	32	-0.15919742991920316
120	33	-0.17672079592320955
120	34	-0.16400315274441368
120	35	-0.11666209539654915
120	36	-0.17630556691469224
120	37	-0.1281876724173543
120	38	-0.1311663631247839
120	39	-0.12679943608593192
120	40	-0.1422893267406522
120	41	-0.10845571945576182
120	42	-0.11776489443851546
120	43	-0.09745213782898077
120	44	-0.08147598311604298
120	45	-0.1237810196083231
120	46	-0.06773343238225493
120	47	-0.01844023016654006
120	48	0.011137917326436764
120	49	0.010742692809451171
120	50	0.026681028294262887
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	5115.0
3	584.0
4	508.0
5	535.0
6	707.0
7	1520.0
8	1906.0
9	2101.0
10	3065.0
11	5000.0
12	5067.0
13	6651.0
14	9402.0
15	11553.0
16	11670.0
17	13584.0
18	17479.0
19	21060.0
20	28288.0
21	22109.0
22	26978.0
23	34651.0
24	38285.0
25	42642.0
26	56567.0
27	71845.0
28	83590.0
29	98054.0
30	121525.0
31	150287.0
32	182788.0
33	232635.0
34	323758.0
35	483409.0
36	785795.0
37	1499720.0
38	3238948.0
39	1.9071141E7
40	782329.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	17.157508900498524	29.51442030150317	25.369302135191646	27.958768662806655
2	23.513422111166772	29.638842350709993	26.42358720013491	20.424148337988324
3	23.108269127854363	28.293948124914365	26.074127415887133	22.523655331344138
4	22.321058020539887	27.291543073926494	27.44452830463193	22.94287060090169
5	23.266903094189832	28.156621515898806	28.023910652263744	20.552564737647614
6	23.342457280985517	27.319367496663045	25.7724890008679	23.565686221483542
7	22.97663854505304	28.02315772925842	27.35289621291004	21.647307512778504
8	23.188505111475184	27.252173165339084	27.271603674643213	22.28771804854252
9	22.094660171838854	26.746120291416847	27.792024915859038	23.36719462088526
10	22.118091717734185	28.293297046566764	26.94655785243953	22.642053383259526
11	22.970917057674374	27.724676498628682	26.842221637908704	22.46218480578824
12	22.80289883359132	27.268797986793004	27.322975707393898	22.605327472221777
13	22.128036121099264	28.18254825590842	26.90195352966486	22.787462093327463
14	22.263835060248933	28.554088479219565	27.006169712991934	22.17590674753957
15	22.016885716911865	27.671386112889664	27.764330775333367	22.547397394865104
16	22.921315258186027	27.325599202249496	27.04038899285309	22.712696546711392
17	22.642558969238948	27.172514774840923	27.100801586565176	23.084124669354953
18	22.067840189333843	27.18246782117272	27.062737387064782	23.68695460242865
19	23.13220384011786	27.48441210175667	26.966883913833158	22.416500144292314
20	22.678734642651474	27.85475871565266	27.156776118586478	22.30973052310939
21	23.007660757363567	27.42473587966383	27.043662177673962	22.52394118529864
22	22.558235054563106	27.814055941095155	27.005834090275194	22.62187491406655
23	22.245039131745482	27.11627290493267	28.278938664888166	22.35974929843368
24	22.233130934704246	27.261632719619133	27.13193944067732	23.3732969049993
25	23.08034413449746	27.022819903360983	26.998780727470056	22.898055234671496
26	23.186468167188067	27.073211993062873	27.416881284132185	22.323438555616878
27	22.437331317491424	27.68605033202769	27.352128604095828	22.524489746385058
28	22.45442718379328	27.794064470409985	27.19441276632788	22.557095579468854
29	23.256608904842288	27.066199952726926	27.270580481679502	22.406610660751287
30	22.40625193114613	27.711070768985756	27.290827895674692	22.591849404193425
31	23.179050798104967	26.981725223767643	27.241562943370422	22.597661034756968
32	22.442205297996136	27.024982179656792	27.249685726188037	23.28312679615904
33	23.162238119803224	26.96931269498571	27.17459710913923	22.693852076071835
34	23.217398722445367	27.041429903874054	27.15033605722457	22.59083531645601
35	22.454476246947692	27.088586148745314	28.002574210222896	22.454363394084094
36	22.410152617670853	26.97436323136734	27.996076606657972	22.619407544303836
37	22.47240856961308	27.020853670786423	27.111712548955975	23.395025210644526
38	22.459350614683103	27.877393668495344	27.200730364429305	22.462525352392245
39	23.212664908650854	26.93770850707406	27.191441571995874	22.658185012279215
40	22.461291659402065	26.984090635463325	27.132119267972566	23.422498437162044
41	22.498377088872164	27.794027322882364	27.219218530691684	22.488377057553784
42	23.260037832207104	26.89684136101849	27.189544630475414	22.65357617629899
43	23.25447226593873	26.955260794092446	27.145103444148177	22.645163495820647
44	22.485740080319065	27.783899885724022	27.230375963776865	22.49998407018005
45	22.402621839381094	27.67678541238444	27.19574660422257	22.72484614401189
46	22.477123967325042	27.02373113623766	27.84982739488549	22.649317501551803
47	23.327059568446952	27.02287213087622	27.168899793815875	22.481168506860953
48	22.453529970922734	26.909601420815466	27.115082621742413	23.521785986519387
49	22.504624908105576	26.98422845233923	27.036391959382033	23.474754680173156
50	23.331736868328644	26.991495390885696	27.1385756028167	22.53819213796896
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	6728.0
1	3787.5
2	847.0
3	733.5
4	620.0
5	749.5
6	879.0
7	1177.0
8	1475.0
9	2772.0
10	4069.0
11	7015.5
12	9962.0
13	16913.5
14	23865.0
15	34985.5
16	46106.0
17	68031.5
18	89957.0
19	120993.5
20	152030.0
21	201271.0
22	250512.0
23	343099.0
24	435686.0
25	464667.0
26	493648.0
27	574736.5
28	655825.0
29	740335.0
30	824845.0
31	922031.5
32	1019218.0
33	1114278.0
34	1209338.0
35	1311602.5
36	1413867.0
37	1490380.5
38	1566894.0
39	1629525.0
40	1692156.0
41	1732887.5
42	1773619.0
43	1791879.5
44	1810140.0
45	1798491.5
46	1786843.0
47	1752748.5
48	1718654.0
49	1686354.5
50	1654055.0
51	1610811.0
52	1567567.0
53	1509022.0
54	1450477.0
55	1370647.0
56	1290817.0
57	1210118.0
58	1129419.0
59	1145046.5
60	1160674.0
61	940847.0
62	721020.0
63	621620.0
64	522220.0
65	443561.0
66	364902.0
67	305721.0
68	246540.0
69	203263.5
70	159987.0
71	131089.5
72	102192.0
73	80941.5
74	59691.0
75	46190.5
76	32690.0
77	25487.5
78	18285.0
79	14407.0
80	10529.0
81	8243.0
82	5957.0
83	4572.0
84	3187.0
85	2549.5
86	1912.0
87	1765.0
88	1618.0
89	1149.0
90	680.0
91	486.0
92	292.0
93	194.5
94	97.0
95	74.5
96	52.0
97	51.0
98	50.0
99	104.0
100	158.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05213719013717421
2	0.008293064986239513
3	0.0
4	3.637309204491015E-6
5	0.0
6	0.0
7	0.0
8	1.8186546022455072E-5
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	5.383217622646702E-4
16	0.005634191957756582
17	0.0
18	0.00397921626971317
19	1.7822815102005971E-4
20	0.001727721872133232
21	9.78436176008083E-4
22	0.0023533390553056865
23	3.0189666397275423E-4
24	0.00387009699357844
25	8.293064986239514E-4
26	0.02168200016797094
27	0.04313848716526343
28	0.05480333778406612
29	0.05167161455899936
30	0.05690570250426192
31	0.04731048082281463
32	0.052464547965578395
33	0.05410861172600834
34	0.05813511301537989
35	0.08531308739133675
36	0.09759264326569841
37	0.09486102405312566
38	0.09462459895483374
39	0.10386336433424093
40	0.09163109347953764
41	0.11980205326832054
42	0.11491350969748462
43	0.09035076063955681
44	0.1041579863798047
45	0.10909745227950349
46	0.1371156450816978
47	0.13741390443646606
48	0.14097483014766274
49	0.14109849866061544
50	0.13983271505745257
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2.7492851E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.208256067961024
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.21298660192404	30.155771788582523
2	16.594249245844825	14.67205639831754
3	5.804836931844086	7.698651525471618
4	2.670731481204122	4.722735248393466
5	1.5291564935903097	3.3800670918312896
6	1.0020908752273885	2.658041400925174
7	0.68399840550848	2.1166863662557147
8	0.49433328355689915	1.7482889905919503
9	0.39469792588193564	1.5704016279193538
>10	2.335495007707735	19.441578484151325
>50	0.21703172388510328	6.516892993844403
>100	0.060230459158132583	4.140334147497531
>500	7.06509250299941E-5	0.023619812004383338
>1k	4.143350006639385E-5	0.050161015129642536
>5k	3.298682791450357E-5	0.10107408927842285
>10k+	1.6493413956897572E-5	1.0036390198056953
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCG	205997	0.7492747841975356	Illumina Paired End PCR Primer 2 (100% over 31bp)
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	69295	0.25204734132520484	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.4549236817964059E-5	3.6373092044910147E-6	0.0	0.0	0.0
2	1.4549236817964059E-5	7.274618408982029E-6	0.0	0.0	0.0
3	1.8186546022455072E-5	1.0911927613473045E-5	0.0	0.0	0.0
4	1.8186546022455072E-5	1.0911927613473045E-5	0.0	0.0	0.0
5	4.001040124940116E-5	1.0911927613473045E-5	0.0	3.6373092044910147E-6	0.0
6	4.728501965838319E-5	1.4549236817964059E-5	0.0	1.0911927613473045E-5	0.0
7	5.092232886287421E-5	1.4549236817964059E-5	0.0	1.4549236817964059E-5	0.0
8	6.910887488532928E-5	1.4549236817964059E-5	0.0	1.8186546022455072E-5	0.0
9	1.0911927613473045E-4	1.8186546022455072E-5	0.0	1.8186546022455072E-5	0.0
10	0.0011021046889607776	1.8186546022455072E-5	0.0	2.5461164431437103E-5	0.0
11	0.001229410511117963	1.8186546022455072E-5	0.0	2.5461164431437103E-5	0.0
12	0.0015240325566817352	1.8186546022455072E-5	0.0	2.5461164431437103E-5	0.0
13	0.001742271108951196	2.182385522694609E-5	0.0	2.5461164431437103E-5	0.0
14	0.0018768515495173636	2.182385522694609E-5	0.0	2.5461164431437103E-5	0.0
15	0.009376983129177835	2.182385522694609E-5	0.0	3.2735782840419134E-5	0.0
16	0.009838921398148195	2.182385522694609E-5	0.0	3.2735782840419134E-5	0.0
17	0.011192000422218852	2.182385522694609E-5	0.0	3.2735782840419134E-5	0.0
18	0.012941546149579031	2.5461164431437103E-5	0.0	3.2735782840419134E-5	0.0
19	0.01705534285985837	2.5461164431437103E-5	0.0	3.2735782840419134E-5	0.0
20	0.7979819917548747	2.9098473635928117E-5	0.0	3.2735782840419134E-5	0.0
21	0.8090539609733454	3.2735782840419134E-5	0.0	3.2735782840419134E-5	0.0
22	0.8121129380143224	3.2735782840419134E-5	0.0	3.6373092044910145E-5	0.0
23	0.8136624317354355	3.2735782840419134E-5	0.0	4.001040124940116E-5	0.0
24	0.8143316966290619	3.2735782840419134E-5	0.0	4.364771045389218E-5	0.0
25	0.8150446092331421	3.2735782840419134E-5	0.0	4.364771045389218E-5	0.0
26	0.8158993768961975	3.6373092044910145E-5	0.0	4.728501965838319E-5	0.0
27	0.8166232014278912	3.6373092044910145E-5	0.0	5.092232886287421E-5	0.0
28	0.8436665953632819	3.6373092044910145E-5	0.0	5.092232886287421E-5	0.0
29	0.8450487728609885	3.6373092044910145E-5	0.0	5.8196947271856234E-5	0.0
30	0.8468419662988026	3.6373092044910145E-5	0.0	6.183425647634724E-5	0.0
31	0.8643119624079729	3.6373092044910145E-5	0.0	6.183425647634724E-5	0.0
32	0.8653849686232977	3.6373092044910145E-5	0.0	6.183425647634724E-5	0.0
33	0.8660214977340837	3.6373092044910145E-5	0.0	6.910887488532928E-5	0.0
34	0.8664943479306675	3.6373092044910145E-5	0.0	7.274618408982029E-5	0.0
35	0.8670544935481591	3.6373092044910145E-5	0.0	7.274618408982029E-5	0.0
36	0.8676110018564462	4.001040124940116E-5	0.0	7.274618408982029E-5	0.0
37	0.8683239144605265	4.364771045389218E-5	0.0	7.638349329431131E-5	0.0
38	0.8690259151369932	4.364771045389218E-5	0.0	7.638349329431131E-5	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATCGGA	26320	0.0	36.420357	21
AGATCGG	26490	0.0	36.137222	20
TGCCGAG	27140	0.0	35.363377	15
ATGCCGA	27220	0.0	35.25925	14
ATCGGAA	27365	0.0	35.008625	22
CGAGATC	27530	0.0	34.806625	18
TCGGAAG	27530	0.0	34.7742	23
GAGCGGT	27495	0.0	34.483665	29
CCGAGAT	27825	0.0	34.428265	17
AAGAGCG	27725	0.0	34.389057	27
GAGATCG	27965	0.0	34.23263	19
AGAGCGG	28405	0.0	33.62069	28
GCGGTTC	28210	0.0	33.607426	31
AGCGGTT	28260	0.0	33.539074	30
GCCGAGA	29485	0.0	32.529255	16
CGGAAGA	30155	0.0	31.80621	24
GAAGAGC	38045	0.0	25.326103	26
GGAAGAG	43830	0.0	22.108496	25
CAACGCA	21550	0.0	20.660822	13
TCAACGC	21815	0.0	20.51063	12
>>END_MODULE