FastQCFastQC Report
Thu 26 May 2016
SRR522116_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR522116_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26341652
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCG2026680.7693822695706404Illumina Paired End PCR Primer 2 (100% over 31bp)
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT568460.2158027142716789No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA262750.036.02118321
AGATCGG263600.035.90600620
TGCCGAG269450.035.25047715
ATGCCGA271150.035.00521514
ATCGGAA273950.034.57706522
CGAGATC275200.034.4750618
GAGATCG277700.034.10914619
CCGAGAT279300.034.0233117
TCGGAAG277950.034.01666623
GAGCGGT277850.033.80031229
AGAGCGG279600.033.70046628
AAGAGCG280250.033.6451527
GCGGTTC285050.032.94478231
AGCGGTT286000.032.8423630
GCCGAGA290250.032.81593316
CGGAAGA304750.031.06905224
GAAGAGC387350.024.61461426
GGAAGAG444250.021.6248725
TCAACGC200100.020.05265612
CAACGCA199600.020.04781313
Produced by FastQC (version 0.11.5)