##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR522116_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	26341652
Sequences flagged as poor quality	0
Sequence length	50
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	39.3551284862468	40.0	39.0	40.0	38.0	40.0
2	39.28615547726468	40.0	39.0	40.0	38.0	40.0
3	39.28147327282283	40.0	39.0	40.0	38.0	40.0
4	39.28126330117792	40.0	39.0	40.0	38.0	40.0
5	39.2794607566754	40.0	39.0	40.0	38.0	40.0
6	39.31025039735549	40.0	39.0	40.0	38.0	40.0
7	39.283892635131615	40.0	39.0	40.0	38.0	40.0
8	39.27335787444159	40.0	39.0	40.0	38.0	40.0
9	39.26086628887209	40.0	39.0	40.0	38.0	40.0
10	39.24893670298279	40.0	39.0	40.0	38.0	40.0
11	39.257640561039985	40.0	39.0	40.0	38.0	40.0
12	39.23332458419844	40.0	39.0	40.0	38.0	40.0
13	39.20657603403158	40.0	39.0	40.0	38.0	40.0
14	39.19365649504443	40.0	39.0	40.0	38.0	40.0
15	39.17785141190082	40.0	39.0	40.0	38.0	40.0
16	39.16252602532294	40.0	39.0	40.0	38.0	40.0
17	39.132105192187645	40.0	39.0	40.0	38.0	40.0
18	39.1077913792195	40.0	39.0	40.0	38.0	40.0
19	39.07887140107993	40.0	39.0	40.0	38.0	40.0
20	39.043688717776696	40.0	39.0	40.0	38.0	40.0
21	39.014290979168656	40.0	39.0	40.0	38.0	40.0
22	38.97373467692915	40.0	39.0	40.0	38.0	40.0
23	38.9451997543662	40.0	39.0	40.0	37.0	40.0
24	38.912693516716416	40.0	39.0	40.0	37.0	40.0
25	38.8808208384197	40.0	39.0	40.0	37.0	40.0
26	38.77720262191605	40.0	39.0	40.0	37.0	40.0
27	38.70028770405136	40.0	39.0	40.0	36.0	40.0
28	38.657900992694	40.0	39.0	40.0	36.0	40.0
29	38.61821881938156	40.0	39.0	40.0	36.0	40.0
30	38.53672115932592	40.0	39.0	40.0	36.0	40.0
31	38.47971922945455	40.0	39.0	40.0	36.0	40.0
32	38.38510564941029	40.0	38.0	40.0	35.0	40.0
33	38.606414510373156	40.0	39.0	40.0	36.0	40.0
34	38.57681735374835	40.0	39.0	40.0	36.0	40.0
35	38.5133165148488	40.0	39.0	40.0	36.0	40.0
36	38.57818226434697	40.0	39.0	40.0	36.0	40.0
37	38.53392558674756	40.0	39.0	40.0	36.0	40.0
38	38.48281835171158	40.0	39.0	40.0	36.0	40.0
39	38.46343847379048	40.0	39.0	40.0	36.0	40.0
40	38.40788952796127	40.0	39.0	40.0	36.0	40.0
41	38.34445857837618	40.0	39.0	40.0	36.0	40.0
42	38.28580599272969	40.0	39.0	40.0	36.0	40.0
43	38.230199988975635	40.0	39.0	40.0	35.0	40.0
44	38.16651947265874	40.0	39.0	40.0	35.0	40.0
45	38.12057421455572	40.0	39.0	40.0	35.0	40.0
46	38.026362317746816	40.0	39.0	40.0	35.0	40.0
47	37.97416449051867	40.0	38.0	40.0	35.0	40.0
48	37.960255264172496	40.0	38.0	40.0	35.0	40.0
49	37.91330107162603	40.0	38.0	40.0	35.0	40.0
50	37.85501163708335	40.0	38.0	40.0	35.0	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1	1	-0.13087684052976556
1	2	-0.138093791498612
1	3	-0.1440978409054452
1	4	-0.13725460877559215
1	5	-0.14142615379434176
1	6	-0.14281315730117683
1	7	-0.13944818383066604
1	8	-0.14040513752595984
1	9	-0.14778768263133202
1	10	-0.14668915168164176
1	11	-0.14740376239041808
1	12	-0.15138134141329118
1	13	-0.1521030160447694
1	14	-0.1590685234816931
1	15	-0.1591135645472761
1	16	-0.1383111045541625
1	17	-0.17765744952917828
1	18	-0.17898584303353005
1	19	-0.18082910419969522
1	20	-0.17109196699352225
1	21	-0.1592536593490479
1	22	-0.19735426353047814
1	23	-0.17232974979058469
1	24	-0.1729306038952032
1	25	-0.17384585723686286
1	26	-0.1459638686383613
1	27	-0.1845876795573389
1	28	-0.164377411767191
1	29	-0.1734022320565316
1	30	-0.17784599628893005
1	31	-0.15598990507691468
1	32	-0.11781884712321045
1	33	-0.12358409330515485
1	34	-0.12717297424273966
1	35	-0.09500691375923509
1	36	-0.1043808350748634
1	37	-0.15355977281088684
1	38	-0.12297623794643187
1	39	-0.09627313935929749
1	40	-0.10756082128984445
1	41	-0.10688753789688832
1	42	-0.1165601504674143
1	43	-0.10281717825466785
1	44	-0.1075882158718926
1	45	-0.1432453448434643
1	46	-0.12067926761631753
1	47	-0.10694560006726306
1	48	-0.043204897907031636
1	49	-0.05602939708566623
1	50	-0.05769154499221685
2	1	-0.10192682265629571
2	2	-0.12193413253187657
2	3	-0.12883645135591593
2	4	-0.11434806680108522
2	5	-0.11560860637621317
2	6	-0.12930211448981055
2	7	-0.12035174600317333
2	8	-0.12893596689943365
2	9	-0.11548064297823402
2	10	-0.1281818547289646
2	11	-0.128206456573146
2	12	-0.13868515778624158
2	13	-0.15372198504039858
2	14	-0.1663032319589206
2	15	-0.16078417441995185
2	16	-0.17653740101293636
2	17	-0.1626519189952873
2	18	-0.17821545610276956
2	19	-0.16380779264866163
2	20	-0.14992951078886563
2	21	-0.12981912460158185
2	22	-0.1274917886063207
2	23	-0.15913290670646063
2	24	-0.15044249237900686
2	25	-0.14490346680158694
2	26	-0.18354299983639066
2	27	-0.1952181853560191
2	28	-0.2351021676523004
2	29	-0.17710121042794214
2	30	-0.212426241929343
2	31	-0.18766716313186294
2	32	-0.15949331132435418
2	33	-0.17680012966265224
2	34	-0.19460164948475267
2	35	-0.18526887593658614
2	36	-0.201466433405038
2	37	-0.1930136935481528
2	38	-0.21504882114978585
2	39	-0.2059238385262887
2	40	-0.202351083660254
2	41	-0.22174396718928335
2	42	-0.2583255219621634
2	43	-0.20336016906750842
2	44	-0.24857670863531922
2	45	-0.252119146755561
2	46	-0.21369935874874812
2	47	-0.1932120442372991
2	48	-0.21101320170505744
2	49	-0.1754069778961025
2	50	-0.2133165359308009
3	1	-0.12340653089988507
3	2	-0.12942431049000902
3	3	-0.14555689641201042
3	4	-0.13213710053705796
3	5	-0.1326520644855762
3	6	-0.14327075697067926
3	7	-0.13155732708939638
3	8	-0.1366844816008026
3	9	-0.13179923848441177
3	10	-0.13764853081769246
3	11	-0.1426724956355443
3	12	-0.1300646511615966
3	13	-0.13120611876031774
3	14	-0.15225845335025667
3	15	-0.162340031113402
3	16	-0.17155621527942344
3	17	-0.16863229655093193
3	18	-0.15903907364176462
3	19	-0.16202558416341617
3	20	-0.19843082843413384
3	21	-0.19030107580154265
3	22	-0.18084970360801123
3	23	-0.21097208124955102
3	24	-0.17482225839985688
3	25	-0.16599656349898595
3	26	-0.17487183871989487
3	27	-0.16426252014435505
3	28	-0.16279108902919148
3	29	-0.15100082297497153
3	30	-0.19536484836041978
3	31	-0.1281589148355451
3	32	-0.15807796557124476
3	33	-0.15126814800511568
3	34	-0.18265014903565202
3	35	-0.18216086208413884
3	36	-0.17618218947016118
3	37	-0.20313503284551615
3	38	-0.1921242335073643
3	39	-0.20421240912642702
3	40	-0.18331128393312213
3	41	-0.17748481248772663
3	42	-0.17156058215365277
3	43	-0.1851840358546255
3	44	-0.19902853925986363
3	45	-0.2069614304820817
3	46	-0.2101000088513345
3	47	-0.2086782087445016
3	48	-0.148682207162544
3	49	-0.16441175321293855
3	50	-0.15534567869887184
4	1	-0.10871950761108451
4	2	-0.13450441639460564
4	3	-0.10902108402482469
4	4	-0.10540759331073701
4	5	-0.10775321006962457
4	6	-0.11970841145246425
4	7	-0.12082084357374612
4	8	-0.12949729652294195
4	9	-0.13991925806105598
4	10	-0.12105455229761475
4	11	-0.12468752306019582
4	12	-0.14595820332074538
4	13	-0.1399136622189303
4	14	-0.15378610497296563
4	15	-0.14767981465298874
4	16	-0.11981361565017323
4	17	-0.14190014061662737
4	18	-0.1498507785306913
4	19	-0.14473357070432513
4	20	-0.11945660923394996
4	21	-0.12900305169382165
4	22	-0.14425040699985914
4	23	-0.12755351149698413
4	24	-0.15518068778027327
4	25	-0.13796753559288533
4	26	-0.14309908590750098
4	27	-0.12990677288936325
4	28	-0.10451908068443316
4	29	-0.1342450873677521
4	30	-0.15069700277331322
4	31	-0.13065819761346376
4	32	-0.12348477451011064
4	33	-0.14308176498310132
4	34	-0.15640635476994902
4	35	-0.1392293524003776
4	36	-0.18815043076249083
4	37	-0.20245749304668692
4	38	-0.21844186143752609
4	39	-0.1703151664589413
4	40	-0.2021979058541703
4	41	-0.16253704836274352
4	42	-0.17692411617137793
4	43	-0.21097401468288268
4	44	-0.1868907286046948
4	45	-0.2057158268484116
4	46	-0.12338598087670505
4	47	-0.1719568693096747
4	48	-0.12067076691538858
4	49	-0.1187357939015854
4	50	-0.11803626214268803
5	1	-0.07029548330947932
5	2	-0.07879846765194287
5	3	-0.0707457105941387
5	4	-0.07904130044023816
5	5	-0.0756256603378418
5	6	-0.09135058069114876
5	7	-0.09143613454233446
5	8	-0.0826593309968402
5	9	-0.085856121751398
5	10	-0.09931450225123228
5	11	-0.09420743294841571
5	12	-0.09617237520850352
5	13	-0.094602806901662
5	14	-0.10180999669787383
5	15	-0.08919349303631918
5	16	-0.09899962901783965
5	17	-0.10985016980591666
5	18	-0.09869627654668989
5	19	-0.09434544078398233
5	20	-0.11452093741974778
5	21	-0.10130862733830526
5	22	-0.10441924448716122
5	23	-0.127428824105003
5	24	-0.08716034828871244
5	25	-0.09119099296495392
5	26	-0.08886249402530666
5	27	-0.09254894151418114
5	28	-0.11886948499538619
5	29	-0.08525509230209138
5	30	-0.10904103725507497
5	31	-0.10483483441740304
5	32	-0.12536721263812467
5	33	-0.12487305413118577
5	34	-0.12199281192123834
5	35	-0.09164890098800527
5	36	-0.11190944028597016
5	37	-0.09574114149408786
5	38	-0.11320613003520208
5	39	-0.14509133585682576
5	40	-0.1380449035428768
5	41	-0.15979016979343896
5	42	-0.20156538868940288
5	43	-0.15944853803757297
5	44	-0.16322901216981478
5	45	-0.1729556185371166
5	46	-0.19028998147493326
5	47	-0.20070126690863077
5	48	-0.10903308753231755
5	49	-0.11383258873625834
5	50	-0.10669144193619218
6	1	-0.07262293695279709
6	2	-0.0861754913118915
6	3	-0.06979980583602696
6	4	-0.08808373599050157
6	5	-0.0644360324608968
6	6	-0.09191680408617486
6	7	-0.10773172915148166
6	8	-0.10911314327975674
6	9	-0.09138488548244084
6	10	-0.10644072507326285
6	11	-0.10436912377899432
6	12	-0.07995552813682139
6	13	-0.09814208340733188
6	14	-0.09075438881512099
6	15	-0.09111497650007294
6	16	-0.1150329763514435
6	17	-0.10553111940757276
6	18	-0.10437847690175772
6	19	-0.12784406441394225
6	20	-0.120243615726352
6	21	-0.10414895304356975
6	22	-0.10895544208487706
6	23	-0.09530340390032421
6	24	-0.10340403240749652
6	25	-0.09685816253515611
6	26	-0.11483702765667658
6	27	-0.07777960859800714
6	28	-0.07470516899877566
6	29	-0.06811025448693897
6	30	-0.08804610705865912
6	31	-0.05244513644539239
6	32	-0.0491752004753252
6	33	-0.059528360823144055
6	34	-0.09155920670242779
6	35	-0.08201111941441752
6	36	-0.08174344882554152
6	37	-0.09944682066404198
6	38	-0.11267632768510794
6	39	-0.09556274847999191
6	40	-0.10699796724777855
6	41	-0.08473366061337373
6	42	-0.08452253396972509
6	43	-0.13437404002096542
6	44	-0.08395529839756932
6	45	-0.15603715981612964
6	46	-0.10148396878911115
6	47	-0.1013401978839994
6	48	-0.10279288415290466
6	49	-0.06310450055939043
6	50	-0.03472318070246416
7	1	-0.06416468914203932
7	2	-0.06855183033125911
7	3	-0.0522028926143534
7	4	-0.0474897672477681
7	5	-0.041364080703651496
7	6	-0.052920031087232644
7	7	-0.061566512806699336
7	8	-0.06426991251809966
7	9	-0.06711301824076799
7	10	-0.06308206320411358
7	11	-0.05742842017222216
7	12	-0.07114373060448997
7	13	-0.07583065284804036
7	14	-0.08557584502081284
7	15	-0.06427031321488386
7	16	-0.07822477991527421
7	17	-0.05866923403718971
7	18	-0.09151347027836465
7	19	-0.08134723424074508
7	20	-0.051409676015268246
7	21	-0.07142152642105515
7	22	-0.06294203649667907
7	23	-0.06370906966294854
7	24	-0.06874294040298423
7	25	-0.07050363156962192
7	26	-0.06045606992412189
7	27	-0.09332396599981507
7	28	-0.05941284444704564
7	29	-0.08345554722883719
7	30	-0.0852412130376976
7	31	-0.052139393065765205
7	32	-0.05133130557574361
7	33	-0.05479787298764904
7	34	-0.06194477476113747
7	35	-0.07310852891114905
7	36	-0.07055134714575217
7	37	-0.0826373223560779
7	38	-0.09947808130604585
7	39	-0.07753843453952669
7	40	-0.10334121917473738
7	41	-0.10417792696263461
7	42	-0.09104845668988304
7	43	-0.09179598017919943
7	44	-0.07837548015000095
7	45	-0.09509958763226223
7	46	-0.05524076757412644
7	47	-0.07572130012946587
7	48	-0.04215497224628706
7	49	0.005894392297143725
7	50	0.023090082794759326
8	1	-0.06148735756455892
8	2	-0.07837925710121851
8	3	-0.0701843524842829
8	4	-0.06250910956309497
8	5	-0.05545002737288485
8	6	-0.0700875678091677
8	7	-0.07202009469661164
8	8	-0.07219580663424097
8	9	-0.06524278025829489
8	10	-0.061159110008375706
8	11	-0.06383904072711033
8	12	-0.07388386215572496
8	13	-0.06180471688652034
8	14	-0.07519923283142305
8	15	-0.07134348338615126
8	16	-0.08215589615017649
8	17	-0.07029449276235766
8	18	-0.06684340789837506
8	19	-0.09442473039130306
8	20	-0.07974641050854103
8	21	-0.07004930965538136
8	22	-0.08980771080589278
8	23	-0.06354573483945813
8	24	-0.06475951745144926
8	25	-0.05379135795654122
8	26	-0.06427296667226301
8	27	-0.05075116232661259
8	28	-0.05050937960417912
8	29	-0.051911482314636714
8	30	-0.04275214479041978
8	31	-0.020943220474919144
8	32	-0.043648830770408154
8	33	-0.015050946860945658
8	34	-0.05334015711525808
8	35	-0.02295570011030179
8	36	-0.05889395122494534
8	37	-0.07294116980709475
8	38	-0.0492152073433374
8	39	-0.05980624058839368
8	40	-0.05495624250627884
8	41	-0.057793377428460246
8	42	-0.046609359579761644
8	43	-0.07396570235808753
8	44	-0.0709532751413704
8	45	-0.0745136362397858
8	46	-0.039126686210281036
8	47	-0.07141924342553807
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112	39	-0.06746523935829885
112	40	-0.0620221431296315
112	41	-0.037247840953234856
112	42	-0.016843571423258652
112	43	0.0018001660319200141
112	44	-0.00333318262094906
112	45	0.003747505604181356
112	46	0.011519100154970374
112	47	0.01332813534141053
112	48	0.0777573744191642
112	49	0.0535318190925409
112	50	0.0502766618074304
113	1	-0.009203165720009565
113	2	-0.024626839854796856
113	3	-0.02783068335867256
113	4	-0.028471470491908235
113	5	-0.019823194559407398
113	6	-0.022914460629735345
113	7	-0.027769563008419595
113	8	-0.04819086665192884
113	9	-0.04317919130468084
113	10	-0.03183458460502919
113	11	-0.042467083754097246
113	12	-0.05211926166930425
113	13	-0.04727583344232755
113	14	-0.04290381351338368
113	15	-0.038826390379419706
113	16	-0.034127979604548386
113	17	-0.02741491351166303
113	18	-0.03572733713242826
113	19	-0.032740855863359286
113	20	-0.015770074608830953
113	21	-0.01787292647093608
113	22	-0.021660511631147017
113	23	-0.027261063786404804
113	24	-0.020186156143083167
113	25	-0.005706694430877235
113	26	-0.012976002648940721
113	27	-0.00382725416925922
113	28	0.015913331592123825
113	29	-0.039171467238652724
113	30	0.015310922546852623
113	31	0.04845345920388411
113	32	0.05628150111328267
113	33	-0.005440576767796301
113	34	0.012646071471436926
113	35	0.021365533385100832
113	36	0.04578538195430326
113	37	0.03294914551634065
113	38	0.04310216332867611
113	39	0.027903413130459853
113	40	0.03295907594473135
113	41	0.056635900620612745
113	42	0.03779400728402749
113	43	0.05557950280039847
113	44	0.03151341615718195
113	45	0.07639659486169847
113	46	0.12148119560333015
113	47	0.12848235715205192
113	48	0.14050062209098257
113	49	0.15631866265099603
113	50	0.15071761893010915
114	1	-0.03589865343484888
114	2	-0.05034660330029794
114	3	-0.042088084981273255
114	4	-0.04539748600826954
114	5	-0.035212178699531194
114	6	-0.04666228432136421
114	7	-0.03216278170980047
114	8	-0.05610311185426298
114	9	-0.04229728092771268
114	10	-0.0544464775106519
114	11	-0.04816260886403967
114	12	-0.05605917338206012
114	13	-0.06093594603639474
114	14	-0.05600416807119757
114	15	-0.04332839732273186
114	16	-0.04048734942739429
114	17	-0.042621052962374506
114	18	-0.04828244600002307
114	19	-0.02166970231991172
114	20	-0.022412897182952918
114	21	-0.053510764190079385
114	22	-0.010817153002484758
114	23	-0.032437894820517954
114	24	-0.011615447210090224
114	25	-0.03883954903434983
114	26	-0.021299784285858436
114	27	-0.022832421434102912
114	28	-0.029678018788743543
114	29	-0.012689289269239623
114	30	-0.036757662909231215
114	31	0.02137043206111855
114	32	-9.285313421969477E-4
114	33	0.004955562448110129
114	34	-0.02015386882683856
114	35	-0.015292190705089581
114	36	-0.011126440813754357
114	37	6.24932966019287E-4
114	38	0.014362989430118489
114	39	0.01438203747745348
114	40	0.0218609087833741
114	41	0.01979092921917669
114	42	0.048646334216421394
114	43	0.04738603333437652
114	44	-0.01823748784783419
114	45	0.024930760946418218
114	46	0.03763165938964619
114	47	0.07292200340302202
114	48	0.10667894740755912
114	49	0.10491326966754144
114	50	0.15753146433873155
115	1	-0.021937054237611164
115	2	-0.03672146787074837
115	3	-0.04819179120870842
115	4	-0.027245004745651613
115	5	-0.032272361082910095
115	6	-0.029665956116076586
115	7	-0.04168949417404377
115	8	-0.026992108734923193
115	9	-0.040210994892447616
115	10	-0.006647665640784339
115	11	-0.027695251780627927
115	12	-0.030734843592284733
115	13	-0.026513799812462935
115	14	-0.03949556405097354
115	15	-0.019774990912232226
115	16	-0.02521582407935341
115	17	-0.004372936362393887
115	18	-0.023843239074402334
115	19	-0.024914503126758802
115	20	-0.02047290324289719
115	21	-0.027597475199719668
115	22	-0.03627408767988527
115	23	-0.010601981359094736
115	24	-0.009148361589623732
115	25	0.013341494075795879
115	26	-0.024654642989595743
115	27	-0.0035754630322060166
115	28	-0.007191013584652239
115	29	-0.0026607695568472423
115	30	0.002872418835600854
115	31	0.0034263888546348653
115	32	0.021730754877978598
115	33	-0.01941818742005097
115	34	-0.002250964401383726
115	35	0.024536969461273372
115	36	-0.03982233910652866
115	37	-0.04660443403820125
115	38	-0.04391267871360327
115	39	-0.04309373079757961
115	40	-0.027483920149343533
115	41	0.002651526083404576
115	42	0.002433742226976676
115	43	-0.005712297577751713
115	44	0.017458856889447816
115	45	0.03153181601542343
115	46	0.01787590962186414
115	47	0.06330110751892448
115	48	0.08034540075954055
115	49	0.09947034357873008
115	50	0.06859304281729095
116	1	-0.03047654401382971
116	2	-0.05424239160124955
116	3	-0.033824438004913304
116	4	-0.03070632039484167
116	5	-0.03148443058056216
116	6	-0.037725970211020865
116	7	-0.04253061906284472
116	8	-0.05021031134892695
116	9	-0.035143482749177224
116	10	-0.02873655621681337
116	11	-0.04982226802612644
116	12	-0.04158468291981876
116	13	-0.05902978040963802
116	14	-0.04896561724535786
116	15	-0.05210290137797813
116	16	-0.04561870182469363
116	17	-0.05696922843473118
116	18	-0.048906658072212394
116	19	-0.04469890329114179
116	20	-0.06137981554230265
116	21	-0.04207717534380606
116	22	-0.03667892947486706
116	23	-0.041439821828753054
116	24	-0.05353028837183871
116	25	-0.04736873268116426
116	26	-0.020414953012355852
116	27	-0.018467514572833466
116	28	-0.03443527038991334
116	29	-0.031881751548652915
116	30	0.004759276372681143
116	31	0.034111594609463225
116	32	-0.002253889224668626
116	33	-0.008338422247767596
116	34	-0.030417086690711415
116	35	-6.35660599250798E-4
116	36	-0.02270046404979098
116	37	-0.03641788233170473
116	38	-0.018385182843886128
116	39	-0.045487676243219255
116	40	-0.009534553018951897
116	41	-0.008556694537134035
116	42	0.018637811935889204
116	43	0.005634248253102214
116	44	0.02584262558711714
116	45	0.05448718257475349
116	46	0.01663581858238672
116	47	-4.089822496951001E-4
116	48	0.04440297877275157
116	49	0.058145699377050164
116	50	0.056937943137640445
117	1	-0.057123922317366294
117	2	-0.03680281990901335
117	3	-0.04964018680029625
117	4	-0.042433404721201384
117	5	-0.059972506176542595
117	6	-0.04918980687542529
117	7	-0.047526883613826953
117	8	-0.04134308808229292
117	9	-0.06044568211103751
117	10	-0.053732667112178945
117	11	-0.04517432782824926
117	12	-0.04444267099840715
117	13	-0.03880996553068883
117	14	-0.035512753856686174
117	15	-0.04790180628245366
117	16	-0.0320307832324076
117	17	-0.051243366560136394
117	18	-0.05014669128123472
117	19	-0.07267575369704105
117	20	-0.045840216628761254
117	21	-0.059638437435488356
117	22	-0.04396567852143107
117	23	-0.07850296636353704
117	24	-0.03977180718809592
117	25	-0.05347773080284668
117	26	-0.03835955676443348
117	27	-0.02327942310989073
117	28	-0.040267059214684764
117	29	-0.04811355771224868
117	30	-0.022587705677267422
117	31	-0.0028011323944809874
117	32	-0.008175637754469278
117	33	-0.045852521768409815
117	34	-0.007042508414734527
117	35	-0.017548196746730582
117	36	-0.0555385998221638
117	37	-0.01890924162917429
117	38	-0.04972215657540602
117	39	-0.07196084303866002
117	40	-0.018114589077882215
117	41	-0.016818770211067147
117	42	0.03217110352880326
117	43	0.016013962023407657
117	44	-0.01937837729239078
117	45	0.010972080572493326
117	46	0.06304043394435865
117	47	0.04837696689805426
117	48	0.0839020048387269
117	49	0.08736275120444503
117	50	0.0863974683870623
118	1	-0.036972155257082306
118	2	-0.06940173207651412
118	3	-0.07158607542609019
118	4	-0.06563795062597677
118	5	-0.04494883603674538
118	6	-0.06417420839449761
118	7	-0.0700121543288077
118	8	-0.05931479107637472
118	9	-0.07000338657277894
118	10	-0.06685120121200328
118	11	-0.059957300252435175
118	12	-0.05192885757326593
118	13	-0.05702866969080844
118	14	-0.06435740978060522
118	15	-0.07873533591386206
118	16	-0.06286614858878892
118	17	-0.06937457895542565
118	18	-0.0590798503193426
118	19	-0.07804496951678885
118	20	-0.05821507867442932
118	21	-0.055771513770295655
118	22	-0.02813243777433172
118	23	-0.04987889464912598
118	24	-0.0714811667674553
118	25	-0.04027759833057587
118	26	-0.027808655159311968
118	27	-0.01780235248777018
118	28	-0.03406259329984351
118	29	0.0030906479905681294
118	30	0.0015452529677020266
118	31	0.03363904302288745
118	32	0.03468172516262058
118	33	-0.006036831162383294
118	34	0.02673999497933721
118	35	-0.019428320835260138
118	36	0.008901696311205853
118	37	0.0168794707557538
118	38	0.0204559006094982
118	39	0.030540743727044628
118	40	0.0018753576338994549
118	41	0.027941036349353965
118	42	-0.03354564608486754
118	43	-0.03821937266449282
118	44	-0.018616440172074533
118	45	-0.03338269194752286
118	46	0.039152183001242236
118	47	-0.06657901886991624
118	48	0.04437464384592715
118	49	0.08470559572713654
118	50	0.0734770940433549
119	1	-0.0694945031127574
119	2	-0.06245271029739996
119	3	-0.08202022332620373
119	4	-0.0728780218377949
119	5	-0.06387724694099006
119	6	-0.08410093128234308
119	7	-0.07938206720983487
119	8	-0.07527415438847385
119	9	-0.07636428623718672
119	10	-0.07669254085296018
119	11	-0.09194414075528812
119	12	-0.10394116584310353
119	13	-0.0915720829114548
119	14	-0.09897496012919049
119	15	-0.0934493147486819
119	16	-0.09253451206033958
119	17	-0.08479648889356639
119	18	-0.08895856298241256
119	19	-0.09673565196757039
119	20	-0.13870671266677448
119	21	-0.09858293678883712
119	22	-0.12249746990878663
119	23	-0.11238864358728762
119	24	-0.08792351997154668
119	25	-0.09706456990736712
119	26	-0.09245585408159229
119	27	-0.10370160571785192
119	28	-0.07906830267477005
119	29	-0.08622307823815589
119	30	-0.05178904709871546
119	31	-0.031086163098414943
119	32	-0.04717862710487708
119	33	-0.04485975516586649
119	34	-0.06190675761357767
119	35	-0.04018201168405966
119	36	-0.04929757813678748
119	37	-0.057462209125020536
119	38	-0.08371685522009642
119	39	-0.0639585575885917
119	40	-0.061159176708351026
119	41	-0.06494826517971575
119	42	-0.08257676296290128
119	43	-0.10239336515913067
119	44	-0.04660313378172276
119	45	-0.04237049416146732
119	46	-0.10487772859917754
119	47	-0.03823831890494489
119	48	-0.007137558962121204
119	49	-0.020620001858290493
119	50	-0.016484666641240153
120	1	-0.07258303932854915
120	2	-0.11009440040390928
120	3	-0.10868596648282391
120	4	-0.09615831106267336
120	5	-0.10675815500994901
120	6	-0.08760181935269173
120	7	-0.09265005297208262
120	8	-0.08402448598630485
120	9	-0.08206107341499091
120	10	-0.10918398395516249
120	11	-0.09732719309914017
120	12	-0.09582543341074512
120	13	-0.099257233400877
120	14	-0.10165043800523677
120	15	-0.09768577395574596
120	16	-0.09657392192818293
120	17	-0.10266212787825424
120	18	-0.09850085985811319
120	19	-0.09368597243710042
120	20	-0.1265811965689494
120	21	-0.11528187782295163
120	22	-0.11397319048327859
120	23	-0.09423298550672854
120	24	-0.11036474952359043
120	25	-0.08460001941779893
120	26	-0.067430024659906
120	27	-0.0715931283804423
120	28	-0.06650468726221703
120	29	-0.05858301812688893
120	30	-0.048214398280727266
120	31	-0.03916573087724373
120	32	-0.013767488049886367
120	33	-0.054167988419564495
120	34	-0.054637814633167636
120	35	-0.044456242452135086
120	36	-0.06164655134368502
120	37	-0.08987985915673136
120	38	-0.04652288047670794
120	39	-0.09523396883339075
120	40	-0.05313501953248334
120	41	-0.049978998439769384
120	42	-0.02049654734177153
120	43	-0.0284773200724473
120	44	-0.00854135049766569
120	45	-0.06496426910344866
120	46	-0.027046924413554052
120	47	0.015623843987917496
120	48	0.07321220661639671
120	49	0.088189725288359
120	50	0.08684622169923983
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	6130.0
3	590.0
4	430.0
5	492.0
6	724.0
7	1368.0
8	1685.0
9	1962.0
10	2715.0
11	4432.0
12	4230.0
13	5921.0
14	8113.0
15	10189.0
16	10565.0
17	11979.0
18	15110.0
19	18852.0
20	26363.0
21	19852.0
22	24490.0
23	31344.0
24	34271.0
25	38085.0
26	50390.0
27	64513.0
28	75318.0
29	88458.0
30	108508.0
31	135616.0
32	164677.0
33	209794.0
34	292763.0
35	439841.0
36	718283.0
37	1375560.0
38	3025819.0
39	1.849762E7
40	814600.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	17.233066911124304	29.223490615452814	24.659048711027456	28.884393762395426
2	23.870484372188585	29.25651120366282	26.128397469370146	20.744606954778448
3	23.37885598050294	27.899326897736792	25.68035504582217	23.041462075938096
4	22.681352710908183	26.921363929642684	26.990706581348807	23.406576778100327
5	23.675219762222962	27.81824010126624	27.684558280551276	20.821981855959528
6	23.715069199152733	26.916914702236593	25.31057277652897	24.0574433220817
7	23.340477928629213	27.6691725898095	26.939174894150277	22.05117458741101
8	23.474906772830927	26.86028964864281	26.96313089918331	22.701672679342956
9	22.42016939560207	26.293092779450582	27.460893492936584	23.825844332010764
10	22.515019179510837	27.936846937314336	26.477329516007575	23.070804367167252
11	23.360482478471738	27.2971717946923	26.47089483985287	22.871450886983094
12	23.192258405053714	26.85238192350275	26.940789438718575	23.014570232724964
13	22.525481697199552	27.813764299976327	26.51853422101241	23.14221978181171
14	22.619419789242393	28.185964918274813	26.625397398453053	22.569217894029737
15	22.379465087848445	27.24279531063442	27.43155027384674	22.946189327670393
16	23.334834319693087	26.93364052111928	26.668455296732326	23.06306986245531
17	22.982788461418004	26.81598399609621	26.733452857959545	23.467774684526244
18	22.44312185260989	26.778942625741468	26.693182366931513	24.084753154717127
19	23.529322663989234	27.055648551544138	26.596288355340697	22.818740429125935
20	23.017808255320944	27.504124205837684	26.778362682215302	22.69970485662607
21	23.37527397258974	27.007958325985953	26.6879587207988	22.928808980625508
22	22.913976308243097	27.445755996285026	26.64416838616762	22.996099309304256
23	22.582426259779385	26.775535740317803	27.912765288643765	22.72927271125905
24	22.56917548246117	26.84675740993691	26.794872476270815	23.789194631331103
25	23.469896425019552	26.64768550659049	26.65274222248054	23.229675845909416
26	23.571606214704925	26.724142448102263	27.042680437971228	22.661570899221587
27	22.7721167420766	27.333479447718005	26.983385989326862	22.911017820878527
28	22.82960957078048	27.457663723747867	26.793128321760673	22.919598383710976
29	23.630716503091435	26.715221082857397	26.91319705750317	22.740865356548
30	22.76253768135881	27.342845914702952	26.898285457815835	22.99633094612241
31	23.56141750606569	26.640835459611672	26.831525415965444	22.966221618357192
32	22.822059749564147	26.657976131274	26.888580061446525	23.631384057715323
33	23.532228856322668	26.601599975327378	26.810732331284154	23.055438837065793
34	23.618526059357254	26.626772910406444	26.786155232783237	22.968545797453068
35	22.81564919584192	26.7161550713172	27.641023203616616	22.827172529224264
36	22.78056962881035	26.576115237517854	27.61895728790919	23.0243578457626
37	22.854948975478	26.642298961301037	26.71398172140914	23.788770341811823
38	22.815976792334574	27.567363566923085	26.831374244200155	22.785285396542182
39	23.58044460131105	26.574590375990407	26.816278193808696	23.028686828889843
40	22.841557557090955	26.588447633540007	26.72776749712076	23.842227312248273
41	22.860700569647737	27.469827232672277	26.84297831379739	22.82649388388259
42	23.656354256550852	26.52813203614439	26.795291036167008	23.02022267113775
43	23.65226706037007	26.588039214859293	26.71818825304335	23.041505471727284
44	22.868026755080116	27.443204584187846	26.830618344898788	22.85815031583325
45	22.77076986772426	27.321772039290742	26.822651857174804	23.08480623581019
46	22.851644419663746	26.64152502047537	27.500327476714183	23.006503083146697
47	23.706397985468822	26.666415270951617	26.81836269315972	22.808824050419844
48	22.825226068788258	26.51214275475008	26.744019095376643	23.918612081085026
49	22.834560268822212	26.640084616712528	26.650991301301286	23.874363813163967
50	23.723579103045715	26.645479733913476	26.733111132068956	22.897830030971864
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	5240.0
1	3061.0
2	882.0
3	758.0
4	634.0
5	692.0
6	750.0
7	1006.0
8	1262.0
9	2162.0
10	3062.0
11	5364.0
12	7666.0
13	12623.0
14	17580.0
15	26168.5
16	34757.0
17	52028.5
18	69300.0
19	96029.5
20	122759.0
21	163637.5
22	204516.0
23	283815.0
24	363114.0
25	387368.5
26	411623.0
27	487875.0
28	564127.0
29	642188.5
30	720250.0
31	811103.0
32	901956.0
33	994758.5
34	1087561.0
35	1190358.0
36	1293155.0
37	1371271.0
38	1449387.0
39	1516555.0
40	1583723.0
41	1626663.5
42	1669604.0
43	1698459.5
44	1727315.0
45	1723821.5
46	1720328.0
47	1694342.0
48	1668356.0
49	1641921.5
50	1615487.0
51	1579636.0
52	1543785.0
53	1496755.5
54	1449726.0
55	1376392.5
56	1303059.0
57	1228279.0
58	1153499.0
59	1173926.0
60	1194353.0
61	987552.0
62	780751.0
63	674097.5
64	567444.0
65	482652.0
66	397860.0
67	334596.5
68	271333.0
69	222591.0
70	173849.0
71	142813.0
72	111777.0
73	88780.0
74	65783.0
75	51161.5
76	36540.0
77	29136.0
78	21732.0
79	16874.5
80	12017.0
81	9156.0
82	6295.0
83	4864.5
84	3434.0
85	2640.0
86	1846.0
87	1443.0
88	1040.0
89	763.5
90	487.0
91	360.0
92	233.0
93	162.5
94	92.0
95	84.5
96	77.0
97	58.5
98	40.0
99	123.0
100	206.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04893770519783649
2	0.014057584543292881
3	1.4425822647721562E-4
4	0.0
5	0.0
6	0.0
7	2.3536868530493076E-4
8	3.1129406766136007E-4
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	6.453657500296489E-5
15	3.5305302795739615E-4
16	0.00453654159579665
17	2.999052603078957E-4
18	0.002961089911900742
19	5.314776764950049E-5
20	8.237903985672577E-4
21	2.1638733971582344E-4
22	0.0025662779236473097
23	0.0020196151706810186
24	0.002551092847176024
25	0.0016893397574305514
26	0.01807783353906581
27	0.0405555429856867
28	0.0529010101568421
29	0.05396396550983212
30	0.0568453337702586
31	0.04555902568297539
32	0.050843432294982865
33	0.04886177981548006
34	0.054632108874568694
35	0.08026451795809922
36	0.09786022531920169
37	0.0922721171777685
38	0.09426136219550695
39	0.0998418777987045
40	0.08761409497020156
41	0.11770712026717231
42	0.10954893793297397
43	0.08869223539966287
44	0.1006011316222688
45	0.10077955627080641
46	0.13073591587953556
47	0.13356793264143038
48	0.13434616781058378
49	0.13925854004904475
50	0.13237970040755226
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2.6341652E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.79223361662385
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.82685033796191	30.140815090964345
2	16.094825499428776	14.096567165795589
3	5.513261381440217	7.243140912166209
4	2.623950148894542	4.5963455167505884
5	1.4973131041046246	3.278534262609098
6	0.9662038936804855	2.538733598000845
7	0.7248167585511901	2.2218941373802434
8	0.540912326708034	1.8950207181847831
9	0.41017738453720964	1.6166325463118316
>10	2.5115731360015494	20.911815585806877
>50	0.2341206522600748	7.056707428874552
>100	0.055844899031900486	3.24922900835167
>500	6.347683500864597E-5	0.023044099603694423
>1k	2.618541756286145E-5	0.026214334898698424
>5k	4.3439390698277884E-5	0.11795450646430437
>10k+	1.7375756269386517E-5	0.9873510878367489
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCG	202668	0.7693822695706404	Illumina Paired End PCR Primer 2 (100% over 31bp)
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	56846	0.2158027142716789	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.5185076471285856E-5	7.592538235642928E-6	0.0	7.592538235642928E-6	0.0
2	2.2777614706928784E-5	1.1388807353464392E-5	0.0	1.898134558910732E-5	0.0
3	2.6573883824750248E-5	2.2777614706928784E-5	0.0	2.6573883824750248E-5	0.0
4	2.6573883824750248E-5	3.796269117821464E-5	0.0	3.796269117821464E-5	0.0
5	4.935149853167903E-5	5.3147767649500496E-5	0.0	4.1758960296036106E-5	0.0
6	5.694403676732196E-5	6.453657500296489E-5	0.0	4.1758960296036106E-5	0.0
7	6.833284412078635E-5	7.592538235642928E-5	0.0	5.694403676732196E-5	0.0
8	8.351792059207221E-5	7.972165147425074E-5	0.0	6.0740305885143425E-5	0.0
9	1.5185076471285855E-4	7.972165147425074E-5	0.0	6.0740305885143425E-5	0.0
10	0.0011996210412315825	8.351792059207221E-5	0.0	6.833284412078635E-5	0.0
11	0.0013211016530018695	9.111045882771513E-5	0.0	7.212911323860781E-5	0.0
12	0.0015906367603671933	1.1388807353464391E-4	0.0	7.212911323860781E-5	3.796269117821464E-6
13	0.001799431561847374	1.1768434265246538E-4	0.0	7.212911323860781E-5	3.796269117821464E-6
14	0.0019740599412671612	1.2527688088810832E-4	0.0	7.212911323860781E-5	3.796269117821464E-6
15	0.009088268268064584	1.3286941912375123E-4	0.0	7.212911323860781E-5	3.796269117821464E-6
16	0.00961974594455959	1.4046195735939416E-4	0.0	7.592538235642928E-5	3.796269117821464E-6
17	0.011005384172564425	1.4046195735939416E-4	0.0	8.351792059207221E-5	3.796269117821464E-6
18	0.012709909006466262	1.4425822647721562E-4	0.0	8.731418970989367E-5	3.796269117821464E-6
19	0.016502381855169904	1.4425822647721562E-4	0.0	8.731418970989367E-5	7.592538235642928E-6
20	0.8176252575199157	1.480544955950371E-4	0.0	1.0249926618117952E-4	1.1388807353464392E-5
21	0.8291583230998573	1.5564703383068E-4	0.0	1.0249926618117952E-4	1.1388807353464392E-5
22	0.8332165347868083	1.5564703383068E-4	0.0	1.1009180441682245E-4	1.1388807353464392E-5
23	0.8350235588868914	1.5564703383068E-4	0.0	1.1768434265246538E-4	1.1388807353464392E-5
24	0.8356992947898636	1.6703584118414442E-4	0.0	1.2907315000592977E-4	1.1388807353464392E-5
25	0.8363256791943041	1.7083211030196588E-4	0.0	1.2907315000592977E-4	1.1388807353464392E-5
26	0.8371342845164002	1.7462837941978733E-4	0.0	1.3286941912375123E-4	1.5185076471285856E-5
27	0.8378479831105505	1.7842464853760881E-4	0.0	1.3286941912375123E-4	1.5185076471285856E-5
28	0.8656442655912393	1.8222091765543027E-4	0.0	1.366656882415727E-4	1.5185076471285856E-5
29	0.8671551807001322	1.898134558910732E-4	0.0	1.4425822647721562E-4	1.5185076471285856E-5
30	0.8692621100605231	2.1259107059800198E-4	0.0	1.5185076471285855E-4	1.898134558910732E-5
31	0.897354501532402	2.1638733971582344E-4	0.0	1.5185076471285855E-4	1.898134558910732E-5
32	0.8988046763354098	2.2397987795146637E-4	0.0	1.5185076471285855E-4	1.898134558910732E-5
33	0.8997043921163335	2.3536868530493076E-4	0.0	1.5185076471285855E-4	1.898134558910732E-5
34	0.900182722025179	2.4675749265839515E-4	0.0	1.5564703383068E-4	1.898134558910732E-5
35	0.9007673474693235	2.4675749265839515E-4	0.0	1.5564703383068E-4	1.898134558910732E-5
36	0.9012760475311116	2.5055376177621664E-4	0.0	1.6323957206632294E-4	1.898134558910732E-5
37	0.9018910431281987	2.5055376177621664E-4	0.0	1.6703584118414442E-4	1.898134558910732E-5
38	0.9026616857591164	2.5055376177621664E-4	0.0	1.7842464853760881E-4	1.898134558910732E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATCGGA	26275	0.0	36.021183	21
AGATCGG	26360	0.0	35.906006	20
TGCCGAG	26945	0.0	35.250477	15
ATGCCGA	27115	0.0	35.005215	14
ATCGGAA	27395	0.0	34.577065	22
CGAGATC	27520	0.0	34.47506	18
GAGATCG	27770	0.0	34.109146	19
CCGAGAT	27930	0.0	34.02331	17
TCGGAAG	27795	0.0	34.016666	23
GAGCGGT	27785	0.0	33.800312	29
AGAGCGG	27960	0.0	33.700466	28
AAGAGCG	28025	0.0	33.64515	27
GCGGTTC	28505	0.0	32.944782	31
AGCGGTT	28600	0.0	32.84236	30
GCCGAGA	29025	0.0	32.815933	16
CGGAAGA	30475	0.0	31.069052	24
GAAGAGC	38735	0.0	24.614614	26
GGAAGAG	44425	0.0	21.62487	25
TCAACGC	20010	0.0	20.052656	12
CAACGCA	19960	0.0	20.047813	13
>>END_MODULE