FastQCFastQC Report
Thu 26 May 2016
SRR522115_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR522115_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25515992
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCG1116670.4376353464917217Illumina Paired End PCR Primer 2 (100% over 31bp)
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT936590.3670599990782252No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG170100.032.6408820
ATGCCGA172650.032.1014314
GATCGGA172700.031.9842821
TGCCGAG175850.031.41651315
CGAGATC178650.030.91026518
ATCGGAA180500.030.51702522
GAGCGGT178450.030.4931829
GAGATCG182400.030.22623819
TCGGAAG187100.029.47571623
CCGAGAT188050.029.47108717
AGAGCGG188150.029.28277228
GCGGTTC186250.029.21614831
AGCGGTT188350.028.98363730
AAGAGCG189950.028.87816827
GCCGAGA197400.028.1312316
CGGAAGA210100.026.21788224
AAGCAGT255050.024.1686231
GAGTACT211300.024.02270120
TCAACGC260900.022.20682512
AGTACTT229900.022.11733221
Produced by FastQC (version 0.11.5)