FastQCFastQC Report
Thu 26 May 2016
SRR522114_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR522114_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26349606
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCG1558500.5914699445600818Illumina Paired End PCR Primer 2 (100% over 31bp)
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT689890.2618217517180333No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG245850.034.7441420
TGCCGAG247700.034.31216415
ATGCCGA247800.034.28039614
GATCGGA249000.034.26991321
CGAGATC257800.033.18082418
ATCGGAA258100.033.16382622
CCGAGAT258150.032.9453317
GAGATCG263050.032.47650519
TCGGAAG259100.032.3803823
GAGCGGT252800.032.2395829
AAGAGCG260400.031.79631627
AGAGCGG261500.031.66262428
GCGGTTC259500.031.50817931
GCCGAGA276450.030.84452816
AGCGGTT264600.030.734730
CGGAAGA288100.028.92371624
GAAGAGC360800.023.27126326
GAGTACT177350.020.96337720
AATGCCG425150.020.04891213
GGAAGAG428950.019.76192525
Produced by FastQC (version 0.11.5)