FastQCFastQC Report
Thu 26 May 2016
SRR522113_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR522113_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31014591
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCTGAGCGGGCTGGCAAGGCAGACCGATATCGTATGCCGTCTTCTGCT1869890.6029065480824816Illumina PCR Primer Index 1 (95% over 24bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1799840.5803204046766246No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1377100.44401681776167873No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT923460.29775017829511274No Hit
GTACATGGGGGAGCCATTGTGGCTCCGGCCGGTTGCGCGGGCCCTCGGAC835820.2694925107991913No Hit
TCTCTGAGCGGGCTGGCAAGGCAGACCGATCTCGTATGCCGTCTTCTGCT765020.24666454572946006Illumina Paired End PCR Primer 2 (96% over 29bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT386740.12469614704898092No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT323270.10423158570751424No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT318860.10280967432393354No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG362650.037.12479435
GACCGAT369900.036.53605324
TCGTATG370850.036.46010232
GGTATCA846400.036.428791
CGTATGC372750.036.2232833
GCGGGCT401350.036.135288
ACCGATA254500.036.01756325
TATCGTA251500.035.9711330
AGACCGA392450.035.87503423
ATGCCGT367750.035.79651336
CCGATAT255400.035.78728526
CCGTCTT365450.035.7317139
TGAGCGG407600.035.715675
ATATCGT254100.035.6008329
AGCGGGC408600.035.5798267
CGATATC257800.035.5322927
GCCGTCT367050.035.52854538
GATATCG257900.035.48381828
CAGACCG402350.035.264822
CGGGCTG412450.035.2374469