SRR522113_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR522113_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	31014591
Sequences flagged as poor quality	0
Sequence length	50
%GC	42

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
TCTCTGAGCGGGCTGGCAAGGCAGACCGATATCGTATGCCGTCTTCTGCT	186989	0.6029065480824816	Illumina PCR Primer Index 1 (95% over 24bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	179984	0.5803204046766246	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	137710	0.44401681776167873	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	92346	0.29775017829511274	No Hit
GTACATGGGGGAGCCATTGTGGCTCCGGCCGGTTGCGCGGGCCCTCGGAC	83582	0.2694925107991913	No Hit
TCTCTGAGCGGGCTGGCAAGGCAGACCGATCTCGTATGCCGTCTTCTGCT	76502	0.24666454572946006	Illumina Paired End PCR Primer 2 (96% over 29bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	38674	0.12469614704898092	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	32327	0.10423158570751424	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	31886	0.10280967432393354	No Hit

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
TATGCCG	36265	37.124794	35
GACCGAT	36990	36.536053	24
TCGTATG	37085	36.460102	32
GGTATCA	84640	36.42879	1
CGTATGC	37275	36.22328	33
GCGGGCT	40135	36.13528	8
ACCGATA	25450	36.017563	25
TATCGTA	25150	35.97113	30
AGACCGA	39245	35.875034	23
ATGCCGT	36775	35.796513	36
CCGATAT	25540	35.787285	26
CCGTCTT	36545	35.73171	39
TGAGCGG	40760	35.71567	5
ATATCGT	25410	35.60083	29
AGCGGGC	40860	35.579826	7
CGATATC	25780	35.53229	27
GCCGTCT	36705	35.528545	38
GATATCG	25790	35.483818	28
CAGACCG	40235	35.2648	22
CGGGCTG	41245	35.237446	9