##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR522113_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	31014591
Sequences flagged as poor quality	0
Sequence length	50
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	39.10815012843471	40.0	39.0	40.0	38.0	40.0
2	38.96994308259619	40.0	39.0	40.0	38.0	40.0
3	38.86385059857794	40.0	39.0	40.0	37.0	40.0
4	38.889303457201805	40.0	39.0	40.0	37.0	40.0
5	38.91282938407925	40.0	39.0	40.0	38.0	40.0
6	39.02118154645341	40.0	39.0	40.0	38.0	40.0
7	38.98208030536337	40.0	39.0	40.0	38.0	40.0
8	38.960059412036095	40.0	39.0	40.0	38.0	40.0
9	38.931622989966236	40.0	39.0	40.0	38.0	40.0
10	38.87654346304293	40.0	39.0	40.0	37.0	40.0
11	38.949606525522135	40.0	39.0	40.0	38.0	40.0
12	38.831368242128356	40.0	39.0	40.0	37.0	40.0
13	38.84123450152865	40.0	39.0	40.0	37.0	40.0
14	38.74856405489919	40.0	39.0	40.0	36.0	40.0
15	38.749851706894994	40.0	39.0	40.0	36.0	40.0
16	38.625031295753665	40.0	38.0	40.0	36.0	40.0
17	38.58797676874088	40.0	38.0	40.0	36.0	40.0
18	38.535705049278256	40.0	38.0	40.0	36.0	40.0
19	38.49848408447495	40.0	38.0	40.0	35.0	40.0
20	38.42388223014129	40.0	38.0	40.0	35.0	40.0
21	38.351488981428126	40.0	38.0	40.0	35.0	40.0
22	38.48435460586922	40.0	38.0	40.0	36.0	40.0
23	38.448356807284675	40.0	38.0	40.0	35.0	40.0
24	38.491457520752085	40.0	38.0	40.0	36.0	40.0
25	38.43868094214107	40.0	38.0	40.0	35.0	40.0
26	38.47538118429484	40.0	39.0	40.0	36.0	40.0
27	38.4039481288017	40.0	39.0	40.0	35.0	40.0
28	38.336044960257574	40.0	38.0	40.0	35.0	40.0
29	38.22027287092066	40.0	38.0	40.0	35.0	40.0
30	38.10104489206387	40.0	38.0	40.0	35.0	40.0
31	37.73434626302181	40.0	38.0	40.0	35.0	40.0
32	37.72826818835044	40.0	38.0	40.0	35.0	40.0
33	37.56995850759405	40.0	38.0	40.0	33.0	40.0
34	37.484221023582094	40.0	38.0	40.0	33.0	40.0
35	37.30100571050574	40.0	38.0	40.0	33.0	40.0
36	37.068122226728704	40.0	38.0	40.0	33.0	40.0
37	36.946929559703044	40.0	38.0	40.0	33.0	40.0
38	36.80631071356059	39.0	38.0	40.0	33.0	40.0
39	36.633253006625175	39.0	38.0	40.0	32.0	40.0
40	36.44913160389573	39.0	37.0	40.0	32.0	40.0
41	36.04190617893365	39.0	37.0	40.0	31.0	40.0
42	35.83665059455403	39.0	36.0	40.0	30.0	40.0
43	35.64690161479157	39.0	36.0	40.0	29.0	40.0
44	35.389277711255325	39.0	36.0	40.0	29.0	40.0
45	35.00633060097423	39.0	36.0	40.0	27.0	40.0
46	34.2752776265855	39.0	35.0	40.0	18.0	40.0
47	34.07006295843141	39.0	35.0	40.0	15.0	40.0
48	33.81824519304478	39.0	35.0	40.0	2.0	40.0
49	33.590267432512654	39.0	35.0	40.0	2.0	40.0
50	33.43851556836587	39.0	35.0	40.0	2.0	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1	1	-0.08184506216374388
1	2	-0.11061023567793882
1	3	-0.11759693234162683
1	4	-0.1321335486167996
1	5	-0.08730747778980685
1	6	-0.08900395202648781
1	7	-0.10730587290398574
1	8	-0.10400840688664204
1	9	-0.08196761445655909
1	10	-0.11450004344499831
1	11	-0.11255362005748282
1	12	-0.10799085445744083
1	13	-0.11153668401710348
1	14	-0.09716995930455141
1	15	-0.13073434346098622
1	16	-0.07012701862898041
1	17	-0.088888337651305
1	18	-0.05729910279185901
1	19	-0.04021354201650951
1	20	-0.03977271867160681
1	21	-0.019667419208794
1	22	-0.07726954928881469
1	23	-0.09674554484805498
1	24	-0.03433660439937114
1	25	-0.01808271775610848
1	26	0.03354003896581048
1	27	0.027591867145638105
1	28	0.054122418445288645
1	29	0.0701550830202251
1	30	0.05666660787121458
1	31	0.1521727398937074
1	32	0.1434766193222785
1	33	0.1720905206819765
1	34	0.1904458448780062
1	35	0.19988302755317733
1	36	0.28789010111575664
1	37	0.290527983361514
1	38	0.2755305598260378
1	39	0.27012199993050956
1	40	0.3250803914723548
1	41	0.36738917049455466
1	42	0.4179226313311233
1	43	0.4190031763498254
1	44	0.4195298260371487
1	45	0.43345309857339487
1	46	0.43559375363353325
1	47	0.49695206203529096
1	48	0.5030632933484043
1	49	0.5512868243345181
1	50	0.5954894313254826
2	1	-0.06188102732210865
2	2	-0.08077557488726228
2	3	-0.06811453238837828
2	4	-0.05144782359671751
2	5	-0.022731102577083107
2	6	-0.0626641963577299
2	7	-0.060490414965109096
2	8	-0.07878321003714461
2	9	-0.027097400486695733
2	10	-0.0782742648488366
2	11	-0.06908265476621267
2	12	-0.07609009027661529
2	13	-0.0688873047358527
2	14	-0.07458439652825177
2	15	-0.07048566478133722
2	16	-0.040337105690767316
2	17	-0.033970184821811245
2	18	-0.02794958571907813
2	19	-0.040715125075657
2	20	-0.03647897729673133
2	21	0.0012203409147062416
2	22	-0.0831009895124808
2	23	-0.06665221334599636
2	24	0.01362038349872563
2	25	0.01988008239683836
2	26	0.051584215898415664
2	27	0.040834539523771696
2	28	0.05539351853768437
2	29	0.09114022048886028
2	30	0.07300037791854663
2	31	0.15056355775435293
2	32	0.15762332271539492
2	33	0.19144259508395578
2	34	0.16892887370058673
2	35	0.1629015579825719
2	36	0.2736741199244719
2	37	0.255997617850376
2	38	0.2709425675771655
2	39	0.33879280610213414
2	40	0.35049103795653735
2	41	0.4064488805912063
2	42	0.41654319187436073
2	43	0.44432293321455063
2	44	0.48445609100672016
2	45	0.4985149936396809
2	46	0.6018057132510961
2	47	0.6259885177040729
2	48	0.6647374662243521
2	49	0.7033621157602497
2	50	0.6611589452916249
3	1	-0.06481375237307674
3	2	-0.08527661727620028
3	3	-0.06838935207591845
3	4	-0.0675002298640095
3	5	-0.07309126349399264
3	6	-0.08653127564326724
3	7	-0.10433521745309093
3	8	-0.06994655619661216
3	9	-0.04972324943361173
3	10	-0.08066213203345995
3	11	-0.051644502411100746
3	12	-0.06716408895594128
3	13	-0.076957241959839
3	14	-0.08380094806167904
3	15	-0.059372917626603794
3	16	-0.03681476389524363
3	17	-0.025510775619167703
3	18	-0.014976580006496931
3	19	-0.010462971267024557
3	20	-0.006343100916240019
3	21	0.033652866464450426
3	22	-0.0019777319529836745
3	23	-0.04041775637322331
3	24	0.04201160766056944
3	25	0.06576318108633927
3	26	0.05300498131051512
3	27	0.0564879458518206
3	28	0.1090613391451285
3	29	0.07610962727162018
3	30	0.10104713938627441
3	31	0.15049424278006285
3	32	0.1771194106399463
3	33	0.1939426079923976
3	34	0.18282662746021572
3	35	0.1889788787274611
3	36	0.2316551726696119
3	37	0.2186612312760161
3	38	0.25722525536191654
3	39	0.3222776815026265
3	40	0.32037586016272
3	41	0.4255707174854564
3	42	0.438545514052457
3	43	0.4115642521769729
3	44	0.4419142114631711
3	45	0.4415271232368383
3	46	0.5383715377190015
3	47	0.5481391312579049
3	48	0.6052210340881601
3	49	0.5742932477662563
3	50	0.5957941920980332
4	1	-0.05585942228744756
4	2	-0.07549637468505921
4	3	-0.08089274786041045
4	4	-0.0644280927634
4	5	-0.09462892989031957
4	6	-0.0833912136520567
4	7	-0.08414910447113755
4	8	-0.0908590541996972
4	9	-0.03386565749798365
4	10	-0.07268679959018698
4	11	-0.06806520860668286
4	12	-0.0765289786395087
4	13	-0.048980263445550065
4	14	-0.05359473664484682
4	15	-0.05361636595502972
4	16	-0.022920482145231347
4	17	-0.02356169814935072
4	18	-0.031170894012547024
4	19	-0.04570166794077579
4	20	-0.021257866486017463
4	21	0.018599169790768144
4	22	-0.026957969322630504
4	23	-0.005245863993728506
4	24	0.031999397817877195
4	25	0.0482095557888087
4	26	0.053861869484990166
4	27	0.07663219827817613
4	28	0.11193193134490542
4	29	0.09951988885057972
4	30	0.12269045910908005
4	31	0.13181948558204226
4	32	0.14512920729679735
4	33	0.16888649368602415
4	34	0.1695660461184616
4	35	0.15751546480926493
4	36	0.2742353325909477
4	37	0.2801336354607997
4	38	0.29339343404068785
4	39	0.34493071056840563
4	40	0.3387309250552306
4	41	0.3894424119852786
4	42	0.39545802983279543
4	43	0.412825744163797
4	44	0.4089794443856505
4	45	0.4588920048287548
4	46	0.4267432295835789
4	47	0.4779919914875066
4	48	0.5047217172946858
4	49	0.49508626418239743
4	50	0.5282634645942039
5	1	-0.1300321628843264
5	2	-0.17708287747114326
5	3	-0.12569868669928752
5	4	-0.17426211347566323
5	5	-0.14265482780847094
5	6	-0.11432984028810012
5	7	-0.09569015306094997
5	8	-0.11043547297516909
5	9	-0.07014076618605714
5	10	-0.12367467102001228
5	11	-0.10042530319663712
5	12	-0.12167371522902926
5	13	-0.10105090028197594
5	14	-0.09188287200106515
5	15	-0.11560716848804731
5	16	-0.07215691090282661
5	17	-0.03724532969184935
5	18	-0.07382661304837512
5	19	-0.08615596193749298
5	20	-0.05577851429919889
5	21	-0.03827522089107305
5	22	-0.02075438440829913
5	23	-0.027754779273550412
5	24	0.04570711580115727
5	25	0.05653718988455125
5	26	0.058722127789280876
5	27	0.09861446549877684
5	28	0.1179898371632433
5	29	0.11704484583247421
5	30	0.12485697797837503
5	31	0.15695071078727807
5	32	0.14060386013179738
5	33	0.1991816783697402
5	34	0.20330361958086485
5	35	0.20243254709536274
5	36	0.2470273729694128
5	37	0.28271922263407134
5	38	0.2790447223743229
5	39	0.31988068736523445
5	40	0.2856423691406462
5	41	0.37707209484842963
5	42	0.45210440984202194
5	43	0.4516040672795043
5	44	0.4804552562362048
5	45	0.46427812512159505
5	46	0.42298350625750203
5	47	0.4268721348018545
5	48	0.4635218744949299
5	49	0.43802216544062844
5	50	0.42958855989649436
6	1	-0.05480495009293662
6	2	-0.05991713116056019
6	3	-0.0759511726030837
6	4	-0.07015763583879675
6	5	-0.04464422323427897
6	6	-0.05999689575858014
6	7	-0.06024127183383143
6	8	-0.06367810176681132
6	9	-0.03420988565354577
6	10	-0.07962896442946032
6	11	-0.06660714463008333
6	12	-0.08178035806657391
6	13	-0.07197012226077248
6	14	-0.06086361987942723
6	15	-0.05842980243065199
6	16	-0.01251972616152841
6	17	-0.0355050242227648
6	18	-0.049844388877176016
6	19	-0.017813194284769907
6	20	-0.017009877200017343
6	21	-0.0061451602407558426
6	22	-0.004510467074524627
6	23	-0.015443354103915397
6	24	0.026488778757467912
6	25	0.05499510020456455
6	26	0.051044540477569456
6	27	0.06394522361056687
6	28	0.056878167298357596
6	29	0.09670674028866699
6	30	0.11719927944535868
6	31	0.12898896639133994
6	32	0.12992682020753676
6	33	0.1789560322568633
6	34	0.20258845273816206
6	35	0.20981450250750555
6	36	0.25227617139303504
6	37	0.2513391233821878
6	38	0.2828269581754057
6	39	0.28541443991755244
6	40	0.30789908358506324
6	41	0.36663234483430784
6	42	0.34534642487562195
6	43	0.35712047686396176
6	44	0.3745422358741237
6	45	0.3836167210941497
6	46	0.3811318176544134
6	47	0.4058365072713741
6	48	0.4237699944954514
6	49	0.48248452249229246
6	50	0.4863617605056092
7	1	-0.053942437785337916
7	2	-0.05614548390883556
7	3	-0.040590378575224406
7	4	-0.05089671132925844
7	5	-0.040629647328728424
7	6	-0.05693362017272818
7	7	-0.059395050770284286
7	8	-0.04665904861055736
7	9	-0.003926524476973725
7	10	-0.07553097990961533
7	11	-0.05626913851386206
7	12	-0.06840982091387104
7	13	-0.049281644305352756
7	14	-0.04548124517687313
7	15	-0.06463574962775454
7	16	-0.021048815940538645
7	17	-0.01635916053383113
7	18	-0.0047226096651726834
7	19	-0.011274960807135415
7	20	0.013762150761884584
7	21	0.013582627324723262
7	22	-0.011361876794822479
7	23	0.008011147702767119
7	24	0.022356216767498438
7	25	0.036619374102315305
7	26	0.034777698018253034
7	27	0.057786935056526545
7	28	0.03826730880955154
7	29	0.051398327115464326
7	30	0.041239927995782466
7	31	0.08601921380557798
7	32	0.07379262848095891
7	33	0.1117509812977815
7	34	0.11611555139469232
7	35	0.17058613440092785
7	36	0.2065052058688508
7	37	0.2040963854960225
7	38	0.219684128456187
7	39	0.21976510095397828
7	40	0.2922664031871207
7	41	0.35497915847005856
7	42	0.3837549804764393
7	43	0.38509308324554326
7	44	0.364209107415725
7	45	0.41248349198964007
7	46	0.503448481003204
7	47	0.5346389835830578
7	48	0.4923858025093466
7	49	0.5215941246215436
7	50	0.5235539653243322
8	1	-0.03259504512640632
8	2	-0.04070686433067294
8	3	-0.07093710582607571
8	4	-0.04882792650612089
8	5	-0.04489620642163317
8	6	-0.0487506838891818
8	7	-0.03858765738553416
8	8	-0.04568045506505314
8	9	-0.01690300495609165
8	10	-0.03925598255067797
8	11	-0.05404192217137904
8	12	-0.07015429064480827
8	13	-0.04672760372547202
8	14	-0.05253379539988856
8	15	-0.025895425586185183
8	16	-0.026431783109181595
8	17	-0.012106477121513137
8	18	-0.0119586290150977
8	19	-0.001968546688985384
8	20	0.023870737519779084
8	21	0.032068603733804935
8	22	0.01022427233606038
8	23	0.017103912954141265
8	24	0.04540425526747782
8	25	0.08386891483721115
8	26	0.07613239519727699
8	27	0.08484592290612625
8	28	0.07407159376186456
8	29	0.10728564323577672
8	30	0.11068557705726079
8	31	0.13053296785184187
8	32	0.16234850342903684
8	33	0.1873421138279241
8	34	0.19014053041964019
8	35	0.19000245446274988
8	36	0.29788265765638045
8	37	0.2840466624227318
8	38	0.2762969267550517
8	39	0.3151222993092304
8	40	0.3280307141182348
8	41	0.3530094633441223
8	42	0.33877959755988485
8	43	0.3676644961559674
8	44	0.39077040147127917
8	45	0.36837726942803073
8	46	0.3425096310252158
8	47	0.40888056214337354
8	48	0.44599319408493443
8	49	0.4694612381386065
8	50	0.4951407605870273
9	1	-0.025022648589072105
9	2	-0.0373796432500626
9	3	-0.04590242043935433
9	4	-0.043618634814528434
9	5	-0.03261883758875683
9	6	-0.04111532946048868
9	7	-0.04664217194832787
9	8	-0.048752620439096006
9	9	-0.04396516046110577
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112	23	0.005791116533274021
112	24	-3.1146709491025604E-4
112	25	0.03936322995503616
112	26	0.058358319437509465
112	27	0.07561375247203017
112	28	0.07523238985499603
112	29	0.1083949861653224
112	30	0.15197562760770467
112	31	0.1853648402480772
112	32	0.18462926954848768
112	33	0.20158665915155893
112	34	0.1993499420649698
112	35	0.23455776730356348
112	36	0.28063655056408265
112	37	0.3108969294399344
112	38	0.3089024857949667
112	39	0.3031178921194524
112	40	0.291107370983994
112	41	0.33142851407587415
112	42	0.370996084560808
112	43	0.3852471945283895
112	44	0.39204837278602866
112	45	0.3832460201636181
112	46	0.46376505181050476
112	47	0.5062549426820269
112	48	0.5364005373551706
112	49	0.5839216606783495
112	50	0.5960303243743965
113	1	0.004588301169128783
113	2	-2.7437500975224793E-4
113	3	0.013922851427288663
113	4	0.068480878917768
113	5	0.043791407662816084
113	6	-0.005264975118684845
113	7	0.013121137514083614
113	8	-0.016297739555234614
113	9	0.047846274019988755
113	10	-0.0010830046450465147
113	11	-0.04577215112770716
113	12	-0.006890550495377568
113	13	-0.04234768658504606
113	14	-0.02026191650355713
113	15	-0.022819837675903898
113	16	0.012264841143085903
113	17	0.007420799724606297
113	18	0.02699481397842618
113	19	0.00556272203185415
113	20	0.008777752495369384
113	21	0.03078349405356562
113	22	0.031805839947665504
113	23	0.022506392644018547
113	24	0.031757856507645954
113	25	0.04316741387115286
113	26	0.06788048096003507
113	27	0.07548634887325534
113	28	0.04269930928435883
113	29	0.10315465309182059
113	30	0.0741724243512536
113	31	0.12656138949252238
113	32	0.10685919775554709
113	33	0.13304387028404818
113	34	0.12370655130862218
113	35	0.16391707807804323
113	36	0.15187583987999176
113	37	0.1819234380087309
113	38	0.17689937544641055
113	39	0.1430896254027587
113	40	0.17840479644861063
113	41	0.27546620720165294
113	42	0.2908631826864365
113	43	0.3336941911344127
113	44	0.3481590775821104
113	45	0.3654346682463654
113	46	0.43610501850066186
113	47	0.4591010817787762
113	48	0.5147764395199346
113	49	0.5364761158564022
113	50	0.6168585241881246
114	1	-0.009546798703574666
114	2	0.002374300644532923
114	3	0.01214597150353569
114	4	0.07196208048921449
114	5	0.0580972408888627
114	6	-0.0023916969725519266
114	7	-0.005027026633563025
114	8	-0.007013293192954961
114	9	0.029370699720452365
114	10	-0.009888764567193675
114	11	-0.04087505214098286
114	12	-0.054411349435014245
114	13	-0.013413449229858543
114	14	-0.028289852837552587
114	15	-0.023513133760324934
114	16	-0.016277789057603798
114	17	-0.013358237428363395
114	18	-0.032108204334150514
114	19	0.002844545671386811
114	20	0.006173293587153239
114	21	0.03122603568860427
114	22	4.0400555910480307E-4
114	23	-0.001281700077008452
114	24	0.02388210252937739
114	25	0.01371105757304747
114	26	0.07925117110060143
114	27	0.08299843042119903
114	28	0.09122554836523022
114	29	0.1287573814492049
114	30	0.14421694614730285
114	31	0.17903200575378264
114	32	0.14896635004726733
114	33	0.18994314102643983
114	34	0.20587213723056408
114	35	0.23237444156315945
114	36	0.2300019224124128
114	37	0.22445995860613266
114	38	0.2620633518803217
114	39	0.24219577568499062
114	40	0.2234714294729443
114	41	0.24822763653737212
114	42	0.3035000091425246
114	43	0.33667836737274115
114	44	0.4016750836205176
114	45	0.4110861803432897
114	46	0.4302642496807465
114	47	0.4444374334734533
114	48	0.44813349086480514
114	49	0.5102688142392111
114	50	0.5012565157356264
115	1	-0.03850161883602965
115	2	-0.05954982692186661
115	3	-0.05431122326024962
115	4	-0.008004313385086448
115	5	-0.06407009672780362
115	6	-0.07105159999311184
115	7	-0.08541961100442563
115	8	-0.07790821487135702
115	9	-0.03734589671019961
115	10	-0.0625283923652944
115	11	-0.08294347253582401
115	12	-0.0921831557290389
115	13	-0.0921197621701424
115	14	-0.060596285720990295
115	15	-0.05979649382912555
115	16	-0.043626099698059306
115	17	-0.038924978250072684
115	18	-0.0747529310804893
115	19	-0.036119102780254764
115	20	-0.02151387343171507
115	21	0.008052137985231411
115	22	-0.027008703519832977
115	23	-0.041418577669482204
115	24	-0.015836993563794977
115	25	0.028241557759656644
115	26	0.009618121081615527
115	27	0.02460148511298854
115	28	0.050114360393649804
115	29	0.03159221630900788
115	30	0.08155974802443211
115	31	0.17836272025595434
115	32	0.16686494689682974
115	33	0.18084930141345978
115	34	0.19420006876607232
115	35	0.21546614271485254
115	36	0.2341208969833275
115	37	0.2555824040314789
115	38	0.2779399791288455
115	39	0.2614029018826258
115	40	0.2567758764827346
115	41	0.3391390009850994
115	42	0.30895281417448217
115	43	0.3722389511391242
115	44	0.3927512501273256
115	45	0.3980148968449413
115	46	0.4336761723873934
115	47	0.45017091940408704
115	48	0.43593046426280324
115	49	0.4749906763156133
115	50	0.5673185144839152
116	1	-0.11982108951782777
116	2	-0.17120589758469862
116	3	-0.11170455967209847
116	4	-0.0819749928509168
116	5	-0.12214793654234768
116	6	-0.1306197214918825
116	7	-0.10360501230766772
116	8	-0.14400768825851884
116	9	-0.08109347704314018
116	10	-0.13726636866071829
116	11	-0.14139485452525946
116	12	-0.13766279010082627
116	13	-0.15948951217286123
116	14	-0.1362321625616829
116	15	-0.14547875053694526
116	16	-0.11202590876786189
116	17	-0.1034412702862042
116	18	-0.11387029873150567
116	19	-0.10018100272225894
116	20	-0.09335524589018718
116	21	-0.06981605176952854
116	22	-0.0340395368803712
116	23	-0.031128093238535826
116	24	0.02122025595305388
116	25	0.0013243651798191536
116	26	0.0769194596142242
116	27	0.0848859835403033
116	28	0.05600318864510001
116	29	0.03789034727654439
116	30	0.06489494712513277
116	31	0.1304486481720275
116	32	0.1511470229361791
116	33	0.1645953580363937
116	34	0.1343423646239188
116	35	0.1725523271739462
116	36	0.19559902008354868
116	37	0.18690234115511828
116	38	0.24216427857206924
116	39	0.2598686969249755
116	40	0.31198315546315314
116	41	0.30494181736094106
116	42	0.3435278156904218
116	43	0.3375574454026662
116	44	0.3388193008824132
116	45	0.36945582898231777
116	46	0.468786415916675
116	47	0.48529049177020767
116	48	0.4873206941719843
116	49	0.5011191934814505
116	50	0.4581921134092326
117	1	-0.031079161561912372
117	2	-0.05776678879513497
117	3	-0.050087648448901234
117	4	0.008618863788932174
117	5	-0.01760829540501163
117	6	-0.09023453789552605
117	7	-0.06219494668408032
117	8	-0.10493196258455129
117	9	-0.016308807949222626
117	10	-0.0675585605000606
117	11	-0.09423181257891144
117	12	-0.09096674226499601
117	13	-0.08923552936428791
117	14	-0.08599706942858631
117	15	-0.08660971708834353
117	16	-0.05357508830412172
117	17	-0.045396869807106555
117	18	-0.04977830712527975
117	19	-0.052812790115766006
117	20	-0.036621767492079016
117	21	-0.015649076687275
117	22	-0.03309508209984102
117	23	-0.02694142594793192
117	24	0.005510008127245669
117	25	-0.019950286938275497
117	26	0.03587236760571244
117	27	0.029733332539088053
117	28	0.019603350531752994
117	29	0.07635693223446793
117	30	0.10065620188760249
117	31	0.1769566386073933
117	32	0.1770769600768034
117	33	0.20488416085595418
117	34	0.15696464426434886
117	35	0.22134962380949474
117	36	0.24091020582905287
117	37	0.24651713146484866
117	38	0.24072884502354697
117	39	0.27589905186108155
117	40	0.3275557578838715
117	41	0.3677304323767885
117	42	0.38416217443635503
117	43	0.43231648534256095
117	44	0.4399395228291425
117	45	0.4727468327656794
117	46	0.5137530822441221
117	47	0.5185175924239189
117	48	0.537439060522388
117	49	0.5255624370259113
117	50	0.5551280092694597
118	1	-0.039890810418661715
118	2	-0.0627299422132026
118	3	-0.05458455194640521
118	4	-0.0041108184089821975
118	5	-0.0499228689210085
118	6	-0.10066159197103985
118	7	-0.10437521011463957
118	8	-0.09703109727085035
118	9	-0.04733488587784507
118	10	-0.0848670505218152
118	11	-0.1274557328453625
118	12	-0.13365893990217614
118	13	-0.10574411906085857
118	14	-0.11772749740049449
118	15	-0.12672857629218015
118	16	-0.21334085679909265
118	17	-0.14456876974658428
118	18	-0.1390225635111264
118	19	-0.15238979408581343
118	20	-0.10773089203290453
118	21	-0.07748915909770204
118	22	-0.12511918190102733
118	23	-0.14478089303941744
118	24	-0.11127572527995966
118	25	-0.11604752934727713
118	26	-0.012029153914760116
118	27	-0.007042596366979126
118	28	-0.023224363095899037
118	29	0.024595392341630884
118	30	0.014554275248798376
118	31	0.0904689367299909
118	32	0.07102105797942215
118	33	0.07120251849764969
118	34	0.08282169998467026
118	35	0.10014213616143763
118	36	0.11567974894258981
118	37	0.12192691936780875
118	38	0.07007326086478116
118	39	0.09362816470312652
118	40	0.15349310243238534
118	41	0.21631824038384195
118	42	0.2014228928912729
118	43	0.2100418610942043
118	44	0.23796767867439428
118	45	0.2499075490260907
118	46	0.23112458384178325
118	47	0.21923192138495295
118	48	0.21284927017044453
118	49	0.23872082668306405
118	50	0.2556832474042494
119	1	-0.00979948922525864
119	2	-0.011221126967200235
119	3	-0.010674435438254193
119	4	0.05459907766309868
119	5	0.022725858970311208
119	6	-0.04223152227294946
119	7	-0.04643993188311413
119	8	-0.05288552006693692
119	9	-0.0018371766567284453
119	10	-0.0333872653637286
119	11	-0.06690743172442382
119	12	-0.06545363510802105
119	13	-0.0655594214962889
119	14	-0.07668171366164955
119	15	-0.03114808517732115
119	16	-0.059233443427473276
119	17	-0.04760794710410465
119	18	-0.05738278204288605
119	19	-0.05940518466678668
119	20	-0.009562732710804767
119	21	0.003478787492234403
119	22	-0.061829888769224794
119	23	-0.03513064781943598
119	24	3.8356854017962405E-4
119	25	0.008426190970034497
119	26	0.04981533561581841
119	27	0.08010816309883495
119	28	0.05918706097997983
119	29	0.12181460483499507
119	30	0.10934530044565349
119	31	0.1802588561954721
119	32	0.18236704753497435
119	33	0.21753312240753786
119	34	0.21746016414495983
119	35	0.21779075441251194
119	36	0.2173084171234052
119	37	0.22556228886455187
119	38	0.21086857332613107
119	39	0.22730954678890214
119	40	0.23574163501360346
119	41	0.2310317743051158
119	42	0.259084764671492
119	43	0.3159066610934502
119	44	0.2999145719905414
119	45	0.3275390176574291
119	46	0.45464511817515785
119	47	0.45801996677468537
119	48	0.47369036590920643
119	49	0.5127469770745279
119	50	0.5698876674126865
120	1	-0.1858899353392971
120	2	-0.21027689651925385
120	3	-0.3076591517870213
120	4	-0.3154156516459281
120	5	-0.30562001839246733
120	6	-0.27194155359081407
120	7	-0.2762977371576767
120	8	-0.2873975202739487
120	9	-0.30366398394249217
120	10	-0.29123257382452294
120	11	-0.17490635733814486
120	12	-0.17549423817970933
120	13	-0.18593076718285317
120	14	-0.19744471365374494
120	15	-0.15309718238717096
120	16	-0.13375396724796218
120	17	-0.12175757808672927
120	18	-0.09483272156389688
120	19	-0.12464921635870496
120	20	-0.10332873175121193
120	21	-0.12801392079384044
120	22	-0.2358058801387486
120	23	-0.2658828941897724
120	24	-0.17985204271599287
120	25	-0.15256897212742615
120	26	-0.2356714882973705
120	27	-0.2381051677183521
120	28	-0.22165436683352624
120	29	-0.21220780446989096
120	30	-0.1727182541530894
120	31	-0.09499210571842553
120	32	-0.1328637229465457
120	33	-0.11299491467172373
120	34	-0.1398373654261249
120	35	-0.16335414026388406
120	36	-0.08800678894068881
120	37	-0.08004162201356735
120	38	-0.2511416555452044
120	39	-0.17067074765058265
120	40	-0.11596522796892828
120	41	-0.14233685087364023
120	42	-0.15365342319457653
120	43	-0.07150818895004107
120	44	-0.06765094358312496
120	45	-0.058994048466317395
120	46	0.024378260437934784
120	47	0.05706212922304843
120	48	0.13233258496916278
120	49	0.2237009269272292
120	50	0.21044276617572422
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	7724.0
3	710.0
4	886.0
5	947.0
6	1406.0
7	2635.0
8	3253.0
9	3844.0
10	5905.0
11	9128.0
12	10145.0
13	14175.0
14	19095.0
15	22622.0
16	24883.0
17	31033.0
18	38803.0
19	47073.0
20	57077.0
21	61721.0
22	79926.0
23	113655.0
24	148235.0
25	125694.0
26	199006.0
27	239812.0
28	251574.0
29	243002.0
30	314809.0
31	387908.0
32	486781.0
33	562068.0
34	818617.0
35	1170169.0
36	1560760.0
37	2565521.0
38	4774773.0
39	1.6535494E7
40	73722.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.76930267412077	19.311960449917805	13.220906153415966	21.697830722545458
2	16.529096258268694	22.810655425894605	37.7235875860204	22.936660729816296
3	18.108850079001634	27.55589086773085	33.152793327827965	21.18246572543956
4	10.963072187539085	16.267317534511417	37.64901816696535	35.12059211098415
5	11.2116924597994	41.50402007623123	35.57456159498631	11.709725868983064
6	29.089026477856596	37.48172637006318	19.110295291928765	14.31895186015146
7	26.208980153889506	34.66016688725639	22.667827539624817	16.46302541922929
8	29.712727793186115	33.602032669075015	20.693256925425842	15.991982612313024
9	27.684514685362128	12.202653260847452	20.76892776048538	39.343904293305044
10	17.3924073349863	28.321885656979966	35.14100185941514	19.144705148618595
11	34.548190559727196	21.741698931319135	23.91662363047122	19.79348687848245
12	20.409774291360847	28.284588419139308	33.0091879994787	18.29644929002115
13	29.912891036734017	20.75666722447873	27.574571895364773	21.755869843422477
14	19.84511463795096	21.016816343354982	32.099787024002126	27.038281994691932
15	23.524008425582654	28.97746741203197	26.300353275656608	21.198170886728764
16	20.900652857230973	27.746978833285276	28.73841541228127	22.61395289720248
17	19.193420928878282	28.132735975786368	30.78633214927774	21.887510946057613
18	19.037242825481723	26.882969373995614	32.87093806911721	21.208849731405454
19	20.66027245047339	26.540237141931033	33.73940349559986	19.06008691199571
20	21.515928748074156	24.939634691459002	34.17850461702796	19.365931943438884
21	22.502291354540212	24.933216518800972	32.981914920440516	19.582577206218296
22	20.824372631578473	24.911058798099255	33.583718708397605	20.680849861924667
23	20.372572380528894	26.082707329592065	33.38306798887014	20.161652301008903
24	20.813324859352107	25.255535771396744	33.89451157312028	20.03662779613087
25	20.078593256039305	26.13327010034518	33.53595361584309	20.252183027772432
26	19.898658640678807	25.127079735361914	33.54490369901153	21.42935792494776
27	20.12102535417877	25.34179101363998	33.30341721207566	21.233766420105592
28	21.156622887050627	25.26552184188433	33.50071479271076	20.07714047835428
29	19.645174661316585	26.3147226993423	33.60131677940124	20.438785859939877
30	19.80387123856459	25.538900326599613	34.09235678084995	20.564871653985854
31	20.350818505882113	26.232467597264485	33.28573386260384	20.13098003424956
32	20.151391313099047	25.600365460665174	34.26585522119809	19.982388005037695
33	20.074909684068725	25.71111338453526	33.50524956714276	20.70872736425325
34	21.13275756590668	25.364556543754237	33.467267116660096	20.03541877367899
35	20.491095593894823	25.68345502441026	33.782716103545695	20.042733278149218
36	20.3049091554142	26.96424948363283	32.35595732842307	20.3748840325299
37	20.58020302069279	25.935199500343327	33.33834181635062	20.146255662613267
38	21.28973992086959	26.278811127108487	31.897710141983193	20.53373881003873
39	20.768668579692793	26.30234986168507	31.871339416288823	21.057642142333314
40	20.636732307964284	26.109711929085073	31.9186167486121	21.33493901433854
41	21.683255722295	26.254671277686803	31.642851436818358	20.419221563199837
42	20.20836493514091	26.54948401810881	32.33768319408532	20.90446785266496
43	20.426278213352838	26.431649193776984	31.296794374128872	21.845278218741306
44	20.604932553789943	26.565676745540834	31.83062198331717	20.99876871735205
45	20.461985314531834	26.892977784622236	32.02011530076249	20.624921600083443
46	20.89334121627083	27.0601081943174	30.12786821146514	21.918682377946627
47	21.412210777853282	27.344866421369485	30.756894202639184	20.486028598138052
48	22.536521090923152	27.58976904061261	28.941656295785094	20.932053572679145
49	21.299124482928843	27.861695639972428	28.94578888279064	21.89339099430808
50	21.22949632656271	27.575449392641456	29.787853371380173	21.407200909415664
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	12363.0
1	11782.0
2	11201.0
3	30699.5
4	50198.0
5	58648.0
6	67098.0
7	54563.5
8	42029.0
9	55229.5
10	68430.0
11	99570.0
12	130710.0
13	228627.5
14	326545.0
15	346157.0
16	365769.0
17	476505.0
18	587241.0
19	564421.5
20	541602.0
21	566229.0
22	590856.0
23	626257.0
24	661658.0
25	707392.5
26	753127.0
27	821417.0
28	889707.0
29	968786.5
30	1047866.0
31	1134735.5
32	1221605.0
33	1306513.0
34	1391421.0
35	1474717.0
36	1558013.0
37	1618788.5
38	1679564.0
39	1710300.0
40	1741036.0
41	1761391.0
42	1781746.0
43	1786800.5
44	1791855.0
45	1766041.5
46	1740228.0
47	1692618.0
48	1645008.0
49	1589078.5
50	1533149.0
51	1472324.0
52	1411499.0
53	1369017.5
54	1326536.0
55	1252431.0
56	1178326.0
57	1198680.0
58	1219034.0
59	1097576.0
60	976118.0
61	841371.0
62	706624.0
63	624602.5
64	542581.0
65	476147.5
66	409714.0
67	339678.0
68	269642.0
69	250881.0
70	232120.0
71	241764.5
72	251409.0
73	190618.0
74	129827.0
75	99213.0
76	68599.0
77	49994.5
78	31390.0
79	23500.0
80	15610.0
81	11687.0
82	7764.0
83	5912.5
84	4061.0
85	2978.0
86	1895.0
87	1426.0
88	957.0
89	712.0
90	467.0
91	345.5
92	224.0
93	156.0
94	88.0
95	61.5
96	35.0
97	28.0
98	21.0
99	23.0
100	25.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.07350411295122351
2	0.010311275747598927
3	1.3864442062124887E-4
4	0.0
5	1.6121444258284755E-5
6	0.0020570962873571346
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	1.6121444258284755E-5
13	0.0018088260457795496
14	9.608380777937713E-4
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0029566728769694238
21	5.96493437556536E-4
22	0.0
23	0.0
24	0.0014186870947290584
25	0.00400779104260959
26	2.5794310813255603E-5
27	3.159803074623812E-4
28	1.6121444258284755E-5
29	0.02001638519108635
30	0.024220857853647013
31	0.027870752833722683
32	0.008937728696793067
33	0.028976683909841018
34	0.04176421349551249
35	0.04943157238475271
36	0.05723757569461419
37	0.0661269400586324
38	0.046339479376013695
39	0.06133242253621851
40	0.05262361834789309
41	0.06403115230505539
42	0.07571919939231184
43	0.07249491054065488
44	0.09879543470362062
45	0.08909354954898487
46	0.0733848142637122
47	0.09803772682348123
48	0.09284984606116521
49	0.10055267212777366
50	0.12038527285431558
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	3.1014591E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.7686531907492
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.59639044112016	22.15042675311541
2	12.726056517227644	8.340314649978126
3	5.997162802194649	5.8955684398073425
4	3.5159769832128	4.608553215582274
5	2.1938247896952046	3.594434184739522
6	1.4780297242125682	2.90598260630042
7	1.03853782920604	2.382204016550838
8	0.7974535571075303	2.090518323886878
9	0.6484237051187445	1.912317436232709
>10	3.5576629047104458	21.162134658536466
>50	0.2712524303254481	6.159928241163712
>100	0.1643588645590748	9.919008445674839
>500	0.010267060675773506	2.258412758651671
>1k	0.004025649672159154	2.3922596433336634
>5k	3.5797714891025456E-4	0.8166529190910651
>10k+	2.1876381304886924E-4	3.4112837073552567
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCTCTGAGCGGGCTGGCAAGGCAGACCGATATCGTATGCCGTCTTCTGCT	186989	0.6029065480824816	Illumina PCR Primer Index 1 (95% over 24bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	179984	0.5803204046766246	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	137710	0.44401681776167873	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	92346	0.29775017829511274	No Hit
GTACATGGGGGAGCCATTGTGGCTCCGGCCGGTTGCGCGGGCCCTCGGAC	83582	0.2694925107991913	No Hit
TCTCTGAGCGGGCTGGCAAGGCAGACCGATCTCGTATGCCGTCTTCTGCT	76502	0.24666454572946006	Illumina Paired End PCR Primer 2 (96% over 29bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	38674	0.12469614704898092	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	32327	0.10423158570751424	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	31886	0.10280967432393354	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	3.224288851656951E-6	0.0
5	0.0	9.672866554970853E-6	0.0	3.224288851656951E-6	0.0
6	0.0	9.672866554970853E-6	0.0	3.224288851656951E-6	0.0
7	0.0	9.672866554970853E-6	0.0	3.224288851656951E-6	0.0
8	0.0	9.672866554970853E-6	0.0	3.224288851656951E-6	0.0
9	0.0	9.672866554970853E-6	0.0	9.672866554970853E-6	0.0
10	0.0	1.2897155406627803E-5	0.0	9.672866554970853E-6	0.0
11	0.0	1.2897155406627803E-5	0.0	9.672866554970853E-6	0.0
12	0.0	1.2897155406627803E-5	0.0	1.2897155406627803E-5	3.224288851656951E-6
13	0.0	1.6121444258284755E-5	0.0	1.9345733109941706E-5	3.224288851656951E-6
14	0.0	1.6121444258284755E-5	0.0	2.5794310813255606E-5	3.224288851656951E-6
15	0.0	1.6121444258284755E-5	0.0	2.5794310813255606E-5	3.224288851656951E-6
16	0.0	1.6121444258284755E-5	0.0	2.9018599664912557E-5	3.224288851656951E-6
17	0.0	1.6121444258284755E-5	0.0	2.9018599664912557E-5	3.224288851656951E-6
18	0.0	1.6121444258284755E-5	0.0	3.546717736822646E-5	3.224288851656951E-6
19	0.0	1.6121444258284755E-5	0.0	4.191575507154036E-5	3.224288851656951E-6
20	3.224288851656951E-6	1.6121444258284755E-5	0.0	7.093435473645292E-5	3.224288851656951E-6
21	3.224288851656951E-6	1.6121444258284755E-5	0.0	9.672866554970852E-5	3.224288851656951E-6
22	3.224288851656951E-6	1.6121444258284755E-5	0.0	1.1607439865965023E-4	3.224288851656951E-6
23	6.448577703313902E-6	1.9345733109941706E-5	0.0	1.6121444258284753E-4	3.224288851656951E-6
24	6.448577703313902E-6	1.9345733109941706E-5	0.0	1.9345733109941705E-4	3.224288851656951E-6
25	6.448577703313902E-6	2.2570021961598656E-5	0.0	2.095787753577018E-4	3.224288851656951E-6
26	6.448577703313902E-6	2.2570021961598656E-5	0.0	2.482702415775852E-4	3.224288851656951E-6
27	6.448577703313902E-6	2.2570021961598656E-5	0.0	2.9663457435243946E-4	3.224288851656951E-6
28	6.448577703313902E-6	2.2570021961598656E-5	0.0	4.191575507154036E-4	3.224288851656951E-6
29	6.448577703313902E-6	2.2570021961598656E-5	0.0	5.771477044465942E-4	3.224288851656951E-6
30	6.448577703313902E-6	2.5794310813255606E-5	0.0	8.125207906175516E-4	9.672866554970853E-6
31	9.672866554970853E-6	2.5794310813255606E-5	0.0	0.0011317253869315897	9.672866554970853E-6
32	9.672866554970853E-6	2.5794310813255606E-5	0.0	0.0016153687146801324	9.672866554970853E-6
33	1.2897155406627803E-5	2.5794310813255606E-5	0.0	0.0024730295492208813	9.672866554970853E-6
34	1.2897155406627803E-5	2.5794310813255606E-5	0.0	0.0037724179564386324	9.672866554970853E-6
35	1.6121444258284755E-5	3.546717736822646E-5	0.0	0.005765028466762628	9.672866554970853E-6
36	1.6121444258284755E-5	3.546717736822646E-5	0.0	0.009250484715403792	9.672866554970853E-6
37	1.6121444258284755E-5	4.191575507154036E-5	0.0	0.014586682764896046	1.2897155406627803E-5
38	1.6121444258284755E-5	4.191575507154036E-5	0.0	0.022334648875427698	1.2897155406627803E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATGCCG	36265	0.0	37.124794	35
GACCGAT	36990	0.0	36.536053	24
TCGTATG	37085	0.0	36.460102	32
GGTATCA	84640	0.0	36.42879	1
CGTATGC	37275	0.0	36.22328	33
GCGGGCT	40135	0.0	36.13528	8
ACCGATA	25450	0.0	36.017563	25
TATCGTA	25150	0.0	35.97113	30
AGACCGA	39245	0.0	35.875034	23
ATGCCGT	36775	0.0	35.796513	36
CCGATAT	25540	0.0	35.787285	26
CCGTCTT	36545	0.0	35.73171	39
TGAGCGG	40760	0.0	35.71567	5
ATATCGT	25410	0.0	35.60083	29
AGCGGGC	40860	0.0	35.579826	7
CGATATC	25780	0.0	35.53229	27
GCCGTCT	36705	0.0	35.528545	38
GATATCG	25790	0.0	35.483818	28
CAGACCG	40235	0.0	35.2648	22
CGGGCTG	41245	0.0	35.237446	9
>>END_MODULE