SRR522112_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR522112_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	36211672
Sequences flagged as poor quality	0
Sequence length	50
%GC	43

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	199837	0.5518579755168445	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	160221	0.44245678575681346	No Hit
TCTCTGAGCGGGCTGGCAAGGCAGACCGATATCGTATGCCGTCTTCTGCT	143597	0.39654893593424795	Illumina PCR Primer Index 1 (95% over 24bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	98590	0.272260281160174	No Hit
TCTCTGAGCGGGCTGGCAAGGCAGACCGATCTCGTATGCCGTCTTCTGCT	73082	0.20181890524138185	Illumina Paired End PCR Primer 2 (96% over 29bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	41967	0.11589357155339305	No Hit

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	89750	36.408028	1
TATGCCG	31035	36.240665	35
GACCGAT	32490	35.491283	24
TCGTATG	31955	35.471863	32
CGTATGC	32500	34.789997	33
ACCGATA	21600	34.463833	25
ATATCGT	21050	34.446304	29
TATCGTA	21055	34.430817	30
GATATCG	21495	34.36412	28
GCGGGCT	36200	34.344646	8
CGATATC	21550	34.28251	27
AGACCGA	35080	34.245876	23
CCGATAT	21620	34.13749	26
TGAGCGG	36985	33.825134	5
CCGTCTT	31970	33.810604	39
ATCGTAT	21885	33.72992	31
GCCGTCT	32180	33.54235	38
CGGGCTG	37215	33.449295	9
ATGCCGT	32690	33.43967	36
AGCGGGC	37780	33.04805	7