##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR522112_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 36211672 Sequences flagged as poor quality 0 Sequence length 50 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.842752552270994 40.0 39.0 40.0 37.0 40.0 2 38.69039524051803 40.0 39.0 40.0 36.0 40.0 3 38.57149772040352 40.0 39.0 40.0 36.0 40.0 4 38.655016923825 40.0 39.0 40.0 36.0 40.0 5 38.66411879572973 40.0 39.0 40.0 36.0 40.0 6 38.73572012913405 40.0 39.0 40.0 36.0 40.0 7 38.6801221716578 40.0 39.0 40.0 36.0 40.0 8 38.641763407113594 40.0 39.0 40.0 36.0 40.0 9 38.65991056695753 40.0 39.0 40.0 36.0 40.0 10 38.55500588318595 40.0 38.0 40.0 36.0 40.0 11 38.61916735024 40.0 38.0 40.0 36.0 40.0 12 38.48072905885152 40.0 38.0 40.0 35.0 40.0 13 38.48901492314412 40.0 38.0 40.0 35.0 40.0 14 38.390626287568274 40.0 38.0 40.0 35.0 40.0 15 38.368572321101325 40.0 38.0 40.0 35.0 40.0 16 38.2389060356009 40.0 38.0 40.0 35.0 40.0 17 38.19842563469591 40.0 38.0 40.0 35.0 40.0 18 38.1234081651905 40.0 38.0 40.0 35.0 40.0 19 38.086412966515326 40.0 38.0 40.0 35.0 40.0 20 38.28586318797983 40.0 38.0 40.0 35.0 40.0 21 38.2972164886504 40.0 38.0 40.0 35.0 40.0 22 38.208465822842975 40.0 38.0 40.0 35.0 40.0 23 38.15097720425613 40.0 38.0 40.0 35.0 40.0 24 38.191288985496165 40.0 38.0 40.0 35.0 40.0 25 38.12803620335454 40.0 38.0 40.0 35.0 40.0 26 38.085414669612604 40.0 38.0 40.0 35.0 40.0 27 38.00036049150119 40.0 38.0 40.0 35.0 40.0 28 37.90784684010172 40.0 38.0 40.0 35.0 40.0 29 37.808719133433 40.0 38.0 40.0 34.0 40.0 30 37.684168933155036 39.0 38.0 40.0 33.0 40.0 31 37.375349196800414 39.0 38.0 40.0 33.0 40.0 32 37.32551150910679 39.0 38.0 40.0 33.0 40.0 33 37.18820470372094 39.0 38.0 40.0 33.0 40.0 34 37.06128443889583 39.0 38.0 40.0 33.0 40.0 35 36.89471571486674 39.0 37.0 40.0 33.0 40.0 36 36.664565502526365 39.0 37.0 40.0 32.0 40.0 37 36.51437185778111 39.0 37.0 40.0 32.0 40.0 38 36.38524603337841 39.0 37.0 40.0 31.0 40.0 39 36.19593585736665 39.0 36.0 40.0 31.0 40.0 40 36.0218631164007 39.0 36.0 40.0 31.0 40.0 41 35.62789442586357 39.0 36.0 40.0 30.0 40.0 42 35.40836976541707 39.0 36.0 40.0 29.0 40.0 43 35.19604767214284 39.0 35.0 40.0 28.0 40.0 44 34.944680184886245 39.0 35.0 40.0 27.0 40.0 45 34.565441413475746 39.0 35.0 40.0 25.0 40.0 46 33.94214879114116 39.0 35.0 40.0 17.0 40.0 47 33.716409559878926 38.0 35.0 40.0 14.0 40.0 48 33.45635432133595 38.0 34.0 40.0 2.0 40.0 49 33.25642466881949 38.0 33.0 40.0 2.0 40.0 50 33.060072895833144 38.0 33.0 40.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1 1 -0.21591419511064913 1 2 -0.20513982251843998 1 3 -0.2040719645683211 1 4 -0.2273830468551239 1 5 -0.23348289575699255 1 6 -0.1956387632774934 1 7 -0.23121866002996683 1 8 -0.21454103940401126 1 9 -0.2026991925691135 1 10 -0.21330923748872266 1 11 -0.20560220667132256 1 12 -0.18465493980421854 1 13 -0.21789959111337254 1 14 -0.19918856996421397 1 15 -0.1788338574061612 1 16 -0.23776083375492618 1 17 -0.19515213448393354 1 18 -0.19427887119123 1 19 -0.1679171648144191 1 20 -0.23562441957373892 1 21 -0.20949035029678953 1 22 -0.2130328512404347 1 23 -0.2068899071686232 1 24 -0.12441661787312341 1 25 -0.14463411645417779 1 26 -0.07734684824224303 1 27 -0.06346334332848613 1 28 -0.09795341197008156 1 29 -0.033791856492058514 1 30 -0.011537258100794645 1 31 0.0041162829359038255 1 32 0.052496897200050796 1 33 0.011574667962634066 1 34 0.042787116829160254 1 35 0.07968384814821405 1 36 0.0489205154669321 1 37 0.07482543030016586 1 38 0.12205842190407168 1 39 0.1153792493463115 1 40 0.1544089665159447 1 41 0.23625148804969598 1 42 0.2279679701699493 1 43 0.26520806343591374 1 44 0.3123959982407598 1 45 0.3482301212596184 1 46 0.37363488341607365 1 47 0.2905608380646143 1 48 0.4279865548470667 1 49 0.3858426716066745 1 50 0.37240136400515667 2 1 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0.4846328011241212 119 48 0.552817759930015 119 49 0.5386530642646647 119 50 0.5324897574778831 120 1 -0.2050892695021176 120 2 -0.23690384634498685 120 3 -0.1994902950379327 120 4 -0.17269374178605545 120 5 -0.23527973162852334 120 6 -0.21627985656054705 120 7 -0.2759488051551173 120 8 -0.19712556622126698 120 9 -0.2659600412234724 120 10 -0.24864943714541 120 11 -0.26726832121834576 120 12 -0.26705020852446637 120 13 -0.21929484244481756 120 14 -0.256843646923258 120 15 -0.2105934897628785 120 16 -0.23529715267232376 120 17 -0.23218960785906262 120 18 -0.2913333343546114 120 19 -0.2127889063365771 120 20 -0.3050244134749178 120 21 -0.3223227676647369 120 22 -0.2500882109581397 120 23 -0.2894669105273735 120 24 -0.19772159674807455 120 25 -0.18798249821037416 120 26 -0.10979151965914724 120 27 -0.17926774490957342 120 28 -0.05393273021682887 120 29 -0.0018904212943340326 120 30 -0.016174090504101457 120 31 -0.017723233306071506 120 32 0.017298423507732252 120 33 0.08733486371625787 120 34 0.0464107039487871 120 35 0.04985161669474536 120 36 0.12906094409921565 120 37 0.11899560514931551 120 38 0.19027147165090952 120 39 0.19320051633270907 120 40 0.19233986386877433 120 41 0.25442934768943815 120 42 0.37343717884935046 120 43 0.2764658355978895 120 44 0.3384669919356824 120 45 0.39395858464444444 120 46 0.4375531603255567 120 47 0.394969885947404 120 48 0.546263656263001 120 49 0.5402888220108437 120 50 0.5410564786778025 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9932.0 3 910.0 4 1239.0 5 1369.0 6 2074.0 7 3847.0 8 4624.0 9 5912.0 10 9199.0 11 13254.0 12 15286.0 13 20726.0 14 27814.0 15 32777.0 16 37603.0 17 46242.0 18 57621.0 19 67987.0 20 79485.0 21 86438.0 22 110986.0 23 147256.0 24 182899.0 25 173187.0 26 256259.0 27 305151.0 28 331208.0 29 343358.0 30 438638.0 31 534685.0 32 659208.0 33 792675.0 34 1128547.0 35 1586780.0 36 2150790.0 37 3508202.0 38 5836305.0 39 1.7172426E7 40 28773.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.4323467286238 19.307975193750295 12.823848540135952 22.43582953748996 2 17.017763718381893 22.70960704418078 36.89605635786471 23.37657287957262 3 18.36771298813777 27.090147860711323 32.638101725425486 21.904037425725413 4 11.36117382262824 16.245496755852642 37.223771385093734 35.16955803642538 5 11.719326298990005 41.216884434389 35.33209126604262 11.731698000578376 6 29.727583693115978 37.29314206823812 18.615720488923838 14.363553749722074 7 26.6949693035082 34.010638121896584 22.48655101435134 16.807841560243887 8 29.92880858967241 33.33861523985968 20.472407349762804 16.260168820705104 9 27.80951125371952 12.028997169752339 20.15645397428763 40.00503760224052 10 16.96756504366879 28.16986467788618 35.20169408360928 19.66087619483574 11 34.75883135139411 21.44900959005704 23.7797636077119 20.012395450836955 12 20.210839753546868 27.684391375244978 32.95175378811561 19.153015083092544 13 30.018452233767473 20.46301028291308 27.326585096257432 22.191952387062017 14 20.357828648063887 20.478801006683554 31.250864019237163 27.912506326015396 15 23.657454977500073 28.985883336179562 25.638042894014944 21.71861879230542 16 21.383157938573383 27.010747926366623 28.259788344329323 23.34630579073067 17 19.934506306350094 27.955125386897727 29.914308090995252 22.196060215756926 18 19.925511862583974 26.57666290581667 31.6428967985792 21.85492843302016 19 21.05301848531048 26.236604043027896 32.77251876135408 19.937858710307548 20 22.040793839652743 24.95259874634078 32.82378751888725 20.182819895119227 21 22.468216501783665 24.97088712117599 32.15621541771227 20.404680959328076 22 21.2020023384733 24.875876996525427 32.65257102270374 21.269549642297537 23 21.129648802509944 25.72526440463622 32.445412499234706 20.699674293619125 24 21.241125300798704 25.281683235017905 32.60832626444813 20.868865199735257 25 20.734588210419595 25.765688205133756 32.747005010277 20.75271857416965 26 20.67387369564217 25.10751736329302 32.60997106644825 21.608637874616562 27 20.547544059988454 25.321909260044823 32.41185385727891 21.718692822687814 28 21.337085958642525 25.241735010383042 32.66490385402694 20.75627517694749 29 20.49608376726878 26.070548260428993 32.626425653394605 20.806942318907623 30 20.54852577572025 25.529181941216667 32.932789843702345 20.989502439360734 31 20.807207244039372 25.87618122474033 32.44295169825508 20.87365983296522 32 20.720024810649754 25.469199262132868 33.077385119267014 20.733390807950368 33 20.82890531406519 25.645357100229432 32.213124582801015 21.312613002904367 34 21.68222240750499 25.32699323693951 32.16526453102675 20.825519824528747 35 21.00681144434059 25.608044871406083 32.72893880028711 20.656204883966215 36 21.155431381683606 26.615508500267886 31.44481107532982 20.784249042718685 37 21.1401349492265 25.672017815625914 32.285828230917886 20.9020190042297 38 21.83192794418669 26.17435851977184 31.175162344041098 20.818551192000374 39 21.076854149878596 26.326827697483196 31.143943614574134 21.452374538064078 40 21.14438903691415 26.024691416706386 31.20817168709592 21.622747859283542 41 21.775475715732647 26.23969357426223 30.84120331795401 21.143627392051116 42 20.96519644511706 26.446495599518915 31.388530125181852 21.199777830182175 43 21.205347882651402 26.255188375440973 30.686325020923206 21.853138720984422 44 21.304685862590617 26.467872077324024 31.015558911893983 21.211883148191387 45 21.305037194736006 26.730382524866634 30.897011757635003 21.067568522762357 46 21.549578632776083 26.77434867995779 29.638822684246463 22.03725000301967 47 21.685917917036985 27.079490716080052 30.03781999339739 21.196771373485575 48 22.549020556381087 27.406845842829313 28.573771917009317 21.470361683780283 49 21.83104085372761 27.32792746659142 28.723739339863403 22.117292339817567 50 21.86163240183111 27.39138910509914 29.237568276259772 21.509410216809982 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 12971.0 1 12368.0 2 11765.0 3 32914.5 4 54064.0 5 64315.0 6 74566.0 7 61900.0 8 49234.0 9 63414.0 10 77594.0 11 113006.5 12 148419.0 13 262993.5 14 377568.0 15 389126.0 16 400684.0 17 515056.5 18 629429.0 19 586633.5 20 543838.0 21 555654.0 22 567470.0 23 594933.5 24 622397.0 25 665354.5 26 708312.0 27 778877.0 28 849442.0 29 938147.0 30 1026852.0 31 1140398.5 32 1253945.0 33 1371037.5 34 1488130.0 35 1599808.5 36 1711487.0 37 1808966.0 38 1906445.0 39 1962175.5 40 2017906.0 41 2056620.0 42 2095334.0 43 2144368.0 44 2193402.0 45 2193546.5 46 2193691.0 47 2149644.0 48 2105597.0 49 2057678.0 50 2009759.0 51 1946209.0 52 1882659.0 53 1825944.5 54 1769230.0 55 1669235.0 56 1569240.0 57 1534691.0 58 1500142.0 59 1384578.0 60 1269014.0 61 1090533.5 62 912053.0 63 797425.5 64 682798.0 65 588032.5 66 493267.0 67 418856.0 68 344445.0 69 294920.5 70 245396.0 71 210785.0 72 176174.0 73 142258.0 74 108342.0 75 84412.5 76 60483.0 77 46194.0 78 31905.0 79 24781.5 80 17658.0 81 13356.5 82 9055.0 83 7007.0 84 4959.0 85 3666.0 86 2373.0 87 1791.5 88 1210.0 89 887.5 90 565.0 91 405.0 92 245.0 93 165.5 94 86.0 95 63.5 96 41.0 97 29.0 98 17.0 99 15.5 100 14.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0676605046019416 2 6.130619983523545E-4 3 0.001187462429241047 4 0.0 5 0.0 6 0.0044598879609867225 7 5.661158092893363E-4 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 9.334007001941251E-4 14 2.595848101131591E-4 15 0.0 16 0.001640355076672516 17 0.00152437037428153 18 0.0 19 0.0 20 2.1263862105014094E-4 21 9.665391865915498E-5 22 3.313848639742457E-5 23 6.158235388854732E-4 24 4.501311068983504E-4 25 0.004689095825235576 26 2.1816170211637838E-4 27 8.367467815349703E-4 28 6.158235388854732E-4 29 0.021390892969537556 30 0.033204763370219416 31 0.031556123671947545 32 0.021716754752445565 33 0.049464714029222395 34 0.04072167670136855 35 0.0578901741957676 36 0.06348505531586611 37 0.07279144691247617 38 0.06122335361924188 39 0.06634048822711086 40 0.07085008391769372 41 0.07379388612599827 42 0.09870022019419596 43 0.08436230174624358 44 0.09943202843547241 45 0.10161088391610308 46 0.08888018205842581 47 0.11355730826237463 48 0.10020802132527876 49 0.10462372463773557 50 0.1156781713918098 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 3.6211672E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 31.705927506712296 #Duplication Level Percentage of deduplicated Percentage of total 1 68.52481119422154 21.726426961351354 2 12.693004606061265 8.048869677642873 3 5.671757644757911 5.394850101610068 4 3.2807635174826113 4.160786010078805 5 2.072900645099327 3.2861618791068206 6 1.3854188955346793 2.635559464095117 7 1.0116506873763569 2.245272634924931 8 0.7541284967942288 1.9128274760082986 9 0.5912261380612204 1.6870835766098276 >10 3.4966599181181244 20.310332196771636 >50 0.29323140957431854 6.5088244864516085 >100 0.2022754615298533 12.312444015524424 >500 0.015908806266373875 3.4116974181979773 >1k 0.005861085721370711 3.1523858492054155 >5k 2.007467015595665E-4 0.4434813304538455 >10k+ 2.00746699190365E-4 2.7629969219672272 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 199837 0.5518579755168445 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 160221 0.44245678575681346 No Hit TCTCTGAGCGGGCTGGCAAGGCAGACCGATATCGTATGCCGTCTTCTGCT 143597 0.39654893593424795 Illumina PCR Primer Index 1 (95% over 24bp) GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 98590 0.272260281160174 No Hit TCTCTGAGCGGGCTGGCAAGGCAGACCGATCTCGTATGCCGTCTTCTGCT 73082 0.20181890524138185 Illumina Paired End PCR Primer 2 (96% over 29bp) ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 41967 0.11589357155339305 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 2.761540533118714E-6 0.0 0.0 0.0 0.0 3 2.761540533118714E-6 0.0 0.0 0.0 0.0 4 2.761540533118714E-6 0.0 0.0 0.0 0.0 5 5.523081066237428E-6 0.0 0.0 0.0 0.0 6 5.523081066237428E-6 0.0 0.0 0.0 0.0 7 8.28462159935614E-6 0.0 0.0 0.0 0.0 8 1.3807702665593569E-5 0.0 0.0 0.0 0.0 9 4.418464852989942E-5 2.761540533118714E-6 0.0 8.28462159935614E-6 0.0 10 4.418464852989942E-5 2.761540533118714E-6 0.0 1.1046162132474856E-5 0.0 11 5.246927012925556E-5 2.761540533118714E-6 0.0 1.1046162132474856E-5 0.0 12 5.246927012925556E-5 2.761540533118714E-6 0.0 1.3807702665593569E-5 0.0 13 5.246927012925556E-5 2.761540533118714E-6 0.0 1.656924319871228E-5 0.0 14 7.732313492732398E-5 5.523081066237428E-6 0.0 2.209232426494971E-5 0.0 15 7.732313492732398E-5 5.523081066237428E-6 0.0 4.694618906301813E-5 0.0 16 8.560775652668012E-5 5.523081066237428E-6 0.0 4.694618906301813E-5 0.0 17 8.560775652668012E-5 5.523081066237428E-6 0.0 6.627697279484912E-5 0.0 18 8.560775652668012E-5 5.523081066237428E-6 0.0 8.008467546044269E-5 0.0 19 1.3531548612281697E-4 5.523081066237428E-6 0.0 9.113083759291755E-5 0.0 20 1.3531548612281697E-4 5.523081066237428E-6 0.0 1.1322316185786726E-4 0.0 21 1.408385671890544E-4 5.523081066237428E-6 0.0 1.3531548612281697E-4 0.0 22 1.408385671890544E-4 5.523081066237428E-6 0.0 1.5188472932152925E-4 0.0 23 1.408385671890544E-4 5.523081066237428E-6 0.0 1.7397705358647897E-4 0.0 24 1.408385671890544E-4 5.523081066237428E-6 0.0 2.3749248584820938E-4 2.761540533118714E-6 25 1.408385671890544E-4 5.523081066237428E-6 0.0 2.485386479806842E-4 2.761540533118714E-6 26 1.436001077221731E-4 5.523081066237428E-6 0.0 2.595848101131591E-4 2.761540533118714E-6 27 1.5188472932152925E-4 5.523081066237428E-6 0.0 3.1205408024241463E-4 2.761540533118714E-6 28 1.5188472932152925E-4 8.28462159935614E-6 0.0 4.446080258321129E-4 2.761540533118714E-6 29 1.5464626985464797E-4 8.28462159935614E-6 0.0 6.103004578192358E-4 2.761540533118714E-6 30 1.5464626985464797E-4 1.1046162132474856E-5 0.0 9.002622137967007E-4 2.761540533118714E-6 31 1.6016935092088539E-4 1.3807702665593569E-5 0.0 0.0012703086452346083 2.761540533118714E-6 32 1.6016935092088539E-4 1.3807702665593569E-5 0.0 0.0018474706166564194 2.761540533118714E-6 33 1.6016935092088539E-4 1.3807702665593569E-5 0.0 0.0028830483165759374 5.523081066237428E-6 34 1.767385941195977E-4 1.3807702665593569E-5 0.0 0.004385326366592518 5.523081066237428E-6 35 1.7950013465271639E-4 1.3807702665593569E-5 0.0 0.006975651386657871 5.523081066237428E-6 36 1.822616751858351E-4 1.3807702665593569E-5 0.0 0.011314031564187371 5.523081066237428E-6 37 1.8502321571895383E-4 1.3807702665593569E-5 0.0 0.01886684492226705 5.523081066237428E-6 38 1.8778475625207252E-4 1.3807702665593569E-5 0.0 0.02960095297449949 5.523081066237428E-6 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 89750 0.0 36.408028 1 TATGCCG 31035 0.0 36.240665 35 GACCGAT 32490 0.0 35.491283 24 TCGTATG 31955 0.0 35.471863 32 CGTATGC 32500 0.0 34.789997 33 ACCGATA 21600 0.0 34.463833 25 ATATCGT 21050 0.0 34.446304 29 TATCGTA 21055 0.0 34.430817 30 GATATCG 21495 0.0 34.36412 28 GCGGGCT 36200 0.0 34.344646 8 CGATATC 21550 0.0 34.28251 27 AGACCGA 35080 0.0 34.245876 23 CCGATAT 21620 0.0 34.13749 26 TGAGCGG 36985 0.0 33.825134 5 CCGTCTT 31970 0.0 33.810604 39 ATCGTAT 21885 0.0 33.72992 31 GCCGTCT 32180 0.0 33.54235 38 CGGGCTG 37215 0.0 33.449295 9 ATGCCGT 32690 0.0 33.43967 36 AGCGGGC 37780 0.0 33.04805 7 >>END_MODULE