##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR522111_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	37150617
Sequences flagged as poor quality	0
Sequence length	50
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.597283996656095	40.0	38.0	40.0	36.0	40.0
2	38.434733829588886	40.0	38.0	40.0	35.0	40.0
3	38.30705350061884	40.0	38.0	40.0	35.0	40.0
4	38.405331168524064	40.0	38.0	40.0	35.0	40.0
5	38.426013570649445	40.0	38.0	40.0	35.0	40.0
6	38.477810718459935	40.0	38.0	40.0	35.0	40.0
7	38.416081595630025	40.0	38.0	40.0	35.0	40.0
8	38.37914576223593	40.0	38.0	40.0	35.0	40.0
9	38.38278099122822	40.0	38.0	40.0	35.0	40.0
10	38.27873604360326	40.0	38.0	40.0	35.0	40.0
11	38.34139753318229	40.0	38.0	40.0	35.0	40.0
12	38.19151181257636	39.0	38.0	40.0	35.0	40.0
13	38.19877513743581	39.0	38.0	40.0	35.0	40.0
14	38.09576357776238	39.0	38.0	40.0	35.0	40.0
15	38.08004254680346	39.0	38.0	40.0	35.0	40.0
16	37.9379836410254	39.0	38.0	40.0	35.0	40.0
17	37.884659923683095	39.0	38.0	40.0	33.0	40.0
18	37.81794493480418	39.0	38.0	40.0	33.0	40.0
19	37.77618498233825	39.0	38.0	40.0	33.0	40.0
20	37.989363245299536	39.0	38.0	40.0	35.0	40.0
21	38.010912254835496	39.0	38.0	40.0	35.0	40.0
22	37.88551355688117	39.0	38.0	40.0	34.0	40.0
23	37.8376178516766	39.0	38.0	40.0	33.0	40.0
24	37.74970528753264	39.0	38.0	40.0	33.0	40.0
25	37.79624446075822	39.0	38.0	40.0	33.0	40.0
26	37.72268299070242	39.0	38.0	40.0	34.0	40.0
27	37.619399780089786	39.0	38.0	40.0	33.0	40.0
28	37.502460107190146	39.0	38.0	40.0	33.0	40.0
29	37.411951596927715	39.0	38.0	40.0	33.0	40.0
30	37.26489336637397	39.0	38.0	40.0	33.0	40.0
31	36.94015264941629	39.0	38.0	40.0	33.0	40.0
32	36.90292599985621	39.0	37.0	40.0	32.0	40.0
33	36.76181932590783	39.0	37.0	40.0	32.0	40.0
34	36.63950800601777	39.0	37.0	40.0	31.0	40.0
35	36.45734360212645	39.0	36.0	40.0	31.0	40.0
36	36.25616906981652	39.0	36.0	40.0	31.0	40.0
37	36.08715532234633	39.0	36.0	40.0	30.0	40.0
38	35.96142696634083	39.0	36.0	40.0	30.0	40.0
39	35.733226611014295	39.0	36.0	40.0	29.0	40.0
40	35.56653850459603	39.0	35.0	40.0	29.0	40.0
41	35.170432162674444	39.0	35.0	40.0	28.0	40.0
42	34.9233959425223	39.0	35.0	40.0	27.0	40.0
43	34.708550923932165	39.0	35.0	40.0	26.0	40.0
44	34.43880740930898	38.0	35.0	40.0	25.0	40.0
45	34.04410136714553	38.0	35.0	40.0	23.0	40.0
46	33.45372503503778	38.0	34.0	40.0	6.0	40.0
47	33.211860680537285	38.0	33.0	40.0	2.0	40.0
48	32.950822054987675	38.0	33.0	40.0	2.0	40.0
49	32.7601768498219	38.0	33.0	40.0	2.0	40.0
50	32.550749910829204	38.0	33.0	40.0	2.0	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1	1	-0.26184479557753804
1	2	-0.2924293435201548
1	3	-0.39012243671756863
1	4	-0.3562256538082238
1	5	-0.37494085723061943
1	6	-0.3176761343139205
1	7	-0.4165806256241069
1	8	-0.342261359373488
1	9	-0.30322755314257677
1	10	-0.36455192979411066
1	11	-0.3624042484041752
1	12	-0.414417559416691
1	13	-0.3210531235483671
1	14	-0.2729269946396542
1	15	-0.31646806634599756
1	16	-0.25179880676674316
1	17	-0.20892309462730907
1	18	-0.18487146965031798
1	19	-0.2043706646725454
1	20	-0.349816571021762
1	21	-0.19098265493206412
1	22	-0.2685221746474227
1	23	-0.324024003713852
1	24	-0.2626073553600037
1	25	-0.21291977890372493
1	26	-0.26225630753950213
1	27	-0.319859616990378
1	28	-0.3215087532920009
1	29	-0.22247177305141008
1	30	-0.23595314493410058
1	31	-0.1281124338174422
1	32	-0.22791484924733396
1	33	-0.09713043674287292
1	34	-0.11904269176186943
1	35	-0.18690328786355792
1	36	-0.10832768004128468
1	37	0.01467740383121452
1	38	-0.13190825296643993
1	39	-0.061207506198634576
1	40	-0.03315175215751509
1	41	0.05841631708312889
1	42	-0.05336867399759626
1	43	0.0456758994044435
1	44	0.07682008525856787
1	45	0.18174689068538186
1	46	0.1789703202063464
1	47	0.30456550283356165
1	48	0.24511059591461049
1	49	0.31061923894303334
1	50	0.21464984956342903
2	1	-0.19422194136542004
2	2	-0.3627106845684551
2	3	-0.25174868159474784
2	4	-0.32069749466484865
2	5	-0.24195735364403248
2	6	-0.33176583518150693
2	7	-0.2695794420732014
2	8	-0.22857731007233184
2	9	-0.31425225845127613
2	10	-0.4282607601048909
2	11	-0.23114156219960336
2	12	-0.22786145565863336
2	13	-0.18679770855715105
2	14	-0.21788773612519208
2	15	-0.17957975894476874
2	16	-0.171877939911262
2	17	-0.15979100401473545
2	18	-0.1012787161866271
2	19	-0.17595784419384586
2	20	-0.31215239483977086
2	21	-0.18367298623728345
2	22	-0.2512274576193789
2	23	-0.20951469607732065
2	24	-0.20653344989595723
2	25	-0.24501547615579966
2	26	-0.23544293692290807
2	27	-0.1696492624496102
2	28	-0.10585140304218754
2	29	-0.10896674130980699
2	30	-0.13928925451422458
2	31	-0.091239935257029
2	32	-0.12289198458150707
2	33	0.05616747094472174
2	34	-0.09495406254451666
2	35	-0.012649386320624956
2	36	0.07342249851527072
2	37	0.15630956075268898
2	38	0.10958535072803244
2	39	0.09504465534379136
2	40	0.09936555712101836
2	41	0.21322587090097045
2	42	0.11757773390731785
2	43	0.2275298177441485
2	44	0.23087550903300524
2	45	0.18195632116857752
2	46	0.3952118622756302
2	47	0.3945304301404704
2	48	0.4112445684350732
2	49	0.45465018091969966
2	50	0.2732349164590957
3	1	-0.18211354278427905
3	2	-0.2541136380642044
3	3	-0.2903386921788851
3	4	-0.3278486211617775
3	5	-0.23309528032345384
3	6	-0.251083982458951
3	7	-0.2424005964021987
3	8	-0.18910148983061248
3	9	-0.31097199830234956
3	10	-0.28063102816276597
3	11	-0.1992234714759178
3	12	-0.20524797076493684
3	13	-0.13235320407701323
3	14	-0.2356921489549464
3	15	-0.17977193268729508
3	16	-0.14038521230177992
3	17	-0.14706410330436626
3	18	-0.1041187862800328
3	19	-0.1290389616887211
3	20	-0.1568669969217069
3	21	-0.12252791776915473
3	22	-0.25063076620017455
3	23	-0.2184857873619208
3	24	-0.1331158135718553
3	25	-0.09869364662447566
3	26	-0.06047228249661174
3	27	-0.18792690523326172
3	28	-0.09411999096491286
3	29	0.040055409989271595
3	30	-0.15521046252892745
3	31	0.06137497715458551
3	32	0.0203998688369893
3	33	0.11694869129067342
3	34	0.10198599359078742
3	35	0.09325231601916784
3	36	0.13041227709096148
3	37	0.1460481528096551
3	38	0.10902430143950426
3	39	0.10648515742342113
3	40	0.21436585123109353
3	41	0.2970815751579394
3	42	0.21671440718085222
3	43	0.3711337477622152
3	44	0.19833461471236546
3	45	0.17768578307870087
3	46	0.4095393297883021
3	47	0.3304585780818172
3	48	0.4014901598590157
3	49	0.5069373289630903
3	50	0.46650150936376633
4	1	-0.23659768081731158
4	2	-0.3161231238948261
4	3	-0.22298663315692835
4	4	-0.3097262826665741
4	5	-0.24780701979987896
4	6	-0.19716370213303236
4	7	-0.19939454770695164
4	8	-0.21202019486921841
4	9	-0.1801363907847673
4	10	-0.21405418134798282
4	11	-0.1761537363246859
4	12	-0.2114735152821794
4	13	-0.14100122697509931
4	14	-0.1814180306353137
4	15	-0.20404044569990987
4	16	-0.18052045368141023
4	17	-0.127028278427737
4	18	-0.1914394925582812
4	19	-0.14070224769277928
4	20	-0.18747871489809853
4	21	-0.1719742323315927
4	22	-0.07717301056383263
4	23	-0.10803707353045411
4	24	-0.1024428747095456
4	25	-0.013142727980479663
4	26	-0.021400706694514327
4	27	-0.03744969310928781
4	28	0.06654907422701939
4	29	0.03342948924547784
4	30	0.10887741360797776
4	31	0.07099484426242952
4	32	0.008533017908668228
4	33	0.10098697392788125
4	34	0.028179962235810763
4	35	0.13114623147991722
4	36	0.19573105761075738
4	37	0.25187567590305804
4	38	0.25935285419131304
4	39	0.22477552364265563
4	40	0.2221139091762936
4	41	0.3395738505284882
4	42	0.35870697827637343
4	43	0.32926087316091923
4	44	0.4068900472461934
4	45	0.37015920807428415
4	46	0.4029551261076705
4	47	0.5427854403163792
4	48	0.5173442814650073
4	49	0.49555817321444806
4	50	0.39599475183703703
5	1	-0.3099836571347012
5	2	-0.38853511893315584
5	3	-0.30338828373860594
5	4	-0.4146176418951413
5	5	-0.3741484937950119
5	6	-0.27327758804988633
5	7	-0.2784630631199292
5	8	-0.2736860277621318
5	9	-0.2343520076329284
5	10	-0.2380813146950871
5	11	-0.23035126998765065
5	12	-0.22413258787852186
5	13	-0.22031703532191216
5	14	-0.19742405046587663
5	15	-0.20434134848612473
5	16	-0.21718197796894145
5	17	-0.19108204723865185
5	18	-0.19824721972027248
5	19	-0.1939749810050273
5	20	-0.19704594211030013
5	21	-0.14239493594300257
5	22	-0.08530300701350768
5	23	-0.11137306777514056
5	24	-0.10112246827394955
5	25	-0.02183869845556785
5	26	0.0028832960798084173
5	27	0.08406736510256962
5	28	0.08046763899387344
5	29	0.015658626214943183
5	30	0.1668203080251942
5	31	0.07832583595893539
5	32	0.14068356701939422
5	33	0.2041201682766598
5	34	0.06470572516620621
5	35	0.11320018050385272
5	36	0.18503738561022232
5	37	0.2724272435577859
5	38	0.23646481451616808
5	39	0.15987844541282215
5	40	0.2712249655977672
5	41	0.3074038413734499
5	42	0.3112079612779226
5	43	0.27892044960022133
5	44	0.3347339226091961
5	45	0.36001226909650086
5	46	0.4053245142616788
5	47	0.5296043163532005
5	48	0.6638182948181495
5	49	0.5665941802270638
5	50	0.4416337976827833
6	1	-0.15641125330353844
6	2	-0.17765138083797893
6	3	-0.18385697744547258
6	4	-0.18292156620450584
6	5	-0.1786741339878546
6	6	-0.15534231771778195
6	7	-0.17362802527011922
6	8	-0.24543125781163866
6	9	-0.18263658466990051
6	10	-0.1796231605044909
6	11	-0.10495406074692681
6	12	-0.08310403403375233
6	13	-0.19361915414718567
6	14	-0.13257327017868192
6	15	-0.1702697001629332
6	16	-0.19525158744961146
6	17	-0.15517930771559918
6	18	-0.11718082952122444
6	19	-0.11738228913786486
6	20	-0.17137561022726544
6	21	-0.1619490048041996
6	22	-0.15738084853337853
6	23	-0.10551572227251427
6	24	-0.0865736341167036
6	25	-0.10410991095405109
6	26	-0.10748089213600309
6	27	-0.037155201601649424
6	28	0.01203737532467386
6	29	0.039303102132230094
6	30	0.015375915904733972
6	31	0.05803684056980529
6	32	0.09261072659139558
6	33	0.11812178535473095
6	34	0.05218569125759842
6	35	0.14471875326088934
6	36	0.20696504292909879
6	37	0.1794804046625984
6	38	0.08397939372400032
6	39	0.09373612599476644
6	40	0.23739005120762613
6	41	0.25899087466864046
6	42	0.3560397215157849
6	43	0.2711023004768407
6	44	0.32333905954158126
6	45	0.5064846706375548
6	46	0.35035856494308604
6	47	0.49483714416050617
6	48	0.5159670683887541
6	49	0.3158464834383281
6	50	0.5556083588847827
7	1	-0.028367951427377136
7	2	-0.08114758421857715
7	3	-0.04061731174988381
7	4	-0.13066317820579343
7	5	-0.08142336023593089
7	6	-0.156175068043666
7	7	-0.12872836313167113
7	8	-0.15401680885939584
7	9	-0.1008722081323441
7	10	-0.15662208939972544
7	11	-0.028145136946712057
7	12	-0.0374892028724787
7	13	-0.07519012381950319
7	14	-0.12971476267718884
7	15	-0.027681723946585635
7	16	0.04995358944584183
7	17	-0.003318080718678118
7	18	-0.0570858422996352
7	19	-0.0717236324169761
7	20	0.02948337693713654
7	21	-0.09091264379672026
7	22	0.05313179924176126
7	23	-0.0588510959268973
7	24	-0.02209150409296967
7	25	-0.12575684989862168
7	26	-0.08663863212473188
7	27	-0.05709470410594264
7	28	-0.03709617592192416
7	29	0.04201408994627798
7	30	0.08697905152229168
7	31	0.14171132193141744
7	32	0.0800040562562998
7	33	0.186896269849683
7	34	0.22161013200006607
7	35	0.1944731763211891
7	36	0.1967664703736247
7	37	0.16886156396103758
7	38	0.3024093678438291
7	39	0.3173782583019715
7	40	0.26697212171250584
7	41	0.24203646790592614
7	42	0.2952109855594074
7	43	0.23783334759339425
7	44	0.32531531364371347
7	45	0.433492033889209
7	46	0.3203255261677853
7	47	0.3270191391554462
7	48	0.44680892391247085
7	49	0.5198495586007041
7	50	0.5095162229323336
8	1	-0.1018298538023501
8	2	-0.09137582582993531
8	3	-0.11080726339307745
8	4	-0.07137259214547953
8	5	-0.12971199525732402
8	6	-0.09390797449759702
8	7	-0.06655349117554721
8	8	-0.04358283664860352
8	9	-0.018555347626673324
8	10	-0.09355147870838465
8	11	-0.16623756303852844
8	12	-0.06813064981191985
8	13	-0.14634352442205056
8	14	-0.06518511410736494
8	15	-0.15832436194485666
8	16	-0.14760126878479696
8	17	-0.14602308248385043
8	18	0.002029326343730986
8	19	-0.09955399119422736
8	20	-0.1665340329609606
8	21	-0.02049227924926811
8	22	-0.17064998146582155
8	23	-0.07859614574027063
8	24	0.003981676215893515
8	25	4.544959917964775E-4
8	26	0.03663015053759722
8	27	0.0488167331706677
8	28	-0.04784266781712887
8	29	-0.023943582102035066
8	30	-0.012203145059650922
8	31	-0.22481530954669182
8	32	-0.187217475337647
8	33	-0.17138011123496
8	34	-0.14817717466907254
8	35	0.06598027085025393
8	36	-0.005503668459901689
8	37	-0.008715996255304503
8	38	0.031216749607388294
8	39	-0.037350214012697336
8	40	0.004976070329860249
8	41	0.05331904839664503
8	42	0.12103103094352718
8	43	0.24116741259465613
8	44	0.03460745102139384
8	45	0.21580218549868135
8	46	-0.02342337708851261
8	47	0.2733720260293282
8	48	0.16249749478211584
8	49	0.22741703451893613
8	50	0.2653356334526933
9	1	-0.053550530102796756
9	2	-0.05608004929982968
9	3	-0.08383479952149742
9	4	-0.07512751643823634
9	5	-0.05635202671126649
9	6	-0.07737157564114483
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113	25	-0.08350878826915675
113	26	0.053605529948356434
113	27	-0.08725009852262389
113	28	-0.08229586165537484
113	29	0.08050898196209744
113	30	0.11807944598736242
113	31	-0.1399366860800697
113	32	0.1979267394644566
113	33	0.18712751622590673
113	34	0.12411295950160905
113	35	0.22130944569609312
113	36	0.21830065393760378
113	37	0.2687738691099355
113	38	0.1731773601366271
113	39	0.32683476836616876
113	40	0.37266563937439656
113	41	0.47163553114484813
113	42	0.4971046342250176
113	43	0.5879991685520807
113	44	0.5564236261682964
113	45	0.543589832423848
113	46	0.71835847464925
113	47	0.7511309220272366
113	48	0.7890803590880822
113	49	0.7393740420559709
113	50	0.7608854914288301
114	1	-0.19512193864982663
114	2	-0.23832926522160847
114	3	-0.13520119893208005
114	4	-0.15316416175051728
114	5	-0.1570965130635429
114	6	-0.11873384682781563
114	7	-0.2097911686616598
114	8	-0.16160166251250985
114	9	-0.17716532289154685
114	10	-0.2497693664538474
114	11	-0.14670283616848678
114	12	-0.24857780398824048
114	13	-0.2617313155676513
114	14	-0.16238485649466838
114	15	-0.2325431612258484
114	16	-0.18754981445434282
114	17	-0.21201460359182533
114	18	-0.13387978793549138
114	19	-0.14538793765636626
114	20	-0.1923408297626139
114	21	-0.16107239856231814
114	22	-0.08814734174546146
114	23	-0.13254926353364027
114	24	-0.17684120402098102
114	25	-0.028934026181879346
114	26	0.04777084627192352
114	27	-0.05882729702172185
114	28	-0.018339348255615562
114	29	-0.05291336231514521
114	30	0.024779476559594116
114	31	0.13950794694486746
114	32	0.19217928147196517
114	33	0.22326891927706782
114	34	0.10409933390772608
114	35	0.22178632317453406
114	36	0.2796359698428432
114	37	0.31276742276623537
114	38	0.3043062282628739
114	39	0.31356473258892237
114	40	0.44738194049656244
114	41	0.39690287572469884
114	42	0.5423454247001942
114	43	0.48261495101609597
114	44	0.5340691821189054
114	45	0.5758461395267531
114	46	0.5244003501001373
114	47	0.6301507705938718
114	48	0.6373280833308073
114	49	0.5820991695485276
114	50	0.6665162721667031
115	1	-0.2798952408675959
115	2	-0.24701975674071974
115	3	-0.28573833260756487
115	4	-0.20499140993892695
115	5	-0.21642622871856787
115	6	-0.2225450815558503
115	7	-0.25001350613369766
115	8	-0.25141546170160467
115	9	-0.207430951891304
115	10	-0.2117476677276855
115	11	-0.2237728114509494
115	12	-0.319116891022837
115	13	-0.2267658234483747
115	14	-0.2669400970051612
115	15	-0.1758624745377233
115	16	-0.23125508855584798
115	17	-0.19184879835638213
115	18	-0.1772474240276054
115	19	-0.1489309973426387
115	20	-0.2663508783718527
115	21	-0.15423145850098763
115	22	-0.23898748372035072
115	23	-0.2472435207110948
115	24	-0.221128392449792
115	25	-0.19501487497376502
115	26	-0.14185726905746066
115	27	-0.20980983422216326
115	28	-0.037654999432419345
115	29	-0.030087180829561078
115	30	-0.05472210004010236
115	31	0.10438348227272343
115	32	0.04627886707220341
115	33	0.1281967664204373
115	34	0.0756636233290564
115	35	0.11336903518915875
115	36	0.21924185540618168
115	37	0.22060578694520672
115	38	0.1453788358689323
115	39	0.30223345711357297
115	40	0.2367605546191598
115	41	0.29162521479650394
115	42	0.49062411109618154
115	43	0.49033560444719626
115	44	0.35465592657899947
115	45	0.6133654630013723
115	46	0.5025757719448478
115	47	0.6106745562323539
115	48	0.7067348616648346
115	49	0.5658349314644369
115	50	0.6310892698159734
116	1	-0.4295817122066623
116	2	-0.4788373956198413
116	3	-0.35131611941433505
116	4	-0.4030661321026656
116	5	-0.41290254993141673
116	6	-0.3558150486376448
116	7	-0.3127738446927424
116	8	-0.31038640379154714
116	9	-0.30318596471770576
116	10	-0.275217305000389
116	11	-0.31514780674654475
116	12	-0.4154330592838491
116	13	-0.34765771242076937
116	14	-0.31899069244876443
116	15	-0.34908129429396695
116	16	-0.3247685059057801
116	17	-0.2193240344579479
116	18	-0.4075542750451291
116	19	-0.3244256189061687
116	20	-0.2837904258352637
116	21	-0.38571343641459777
116	22	-0.2346812944410459
116	23	-0.21175820148744862
116	24	-0.2831578590405286
116	25	-0.14654277986151243
116	26	-0.19320357380546227
116	27	-0.1709081407946087
116	28	-0.11614048824554146
116	29	-0.03814653723888739
116	30	-0.004557770054702814
116	31	0.03955538834161132
116	32	0.19266147877945627
116	33	0.11408589591713536
116	34	0.10778294059485205
116	35	0.2283878291670689
116	36	0.22709605138474132
116	37	0.2464873644497061
116	38	0.21197571227022394
116	39	0.3194163745914338
116	40	0.1897157528996516
116	41	0.24425247287471308
116	42	0.18703201634721012
116	43	0.33226646829443496
116	44	0.39968403325934787
116	45	0.4268744053777951
116	46	0.4904791560000916
116	47	0.5484089669536658
116	48	0.32556709554872043
116	49	0.47655682715031844
116	50	0.45012274583090317
117	1	-0.19477997672569813
117	2	-0.3336439504982849
117	3	-0.2462213424944366
117	4	-0.21907170627354589
117	5	-0.256433123689483
117	6	-0.31078318609734623
117	7	-0.2831607847230231
117	8	-0.38968454418288445
117	9	-0.3034255836998554
117	10	-0.3489031060082297
117	11	-0.42628108457825675
117	12	-0.2512363581647392
117	13	-0.4019218743173454
117	14	-0.3053758132560773
117	15	-0.3893737623507292
117	16	-0.2499413125882839
117	17	-0.2980836901597925
117	18	-0.26146201395361146
117	19	-0.29979406673905373
117	20	-0.2825863398690984
117	21	-0.41010527500395
117	22	-0.2598453219869583
117	23	-0.26564846344178505
117	24	-0.4371788056621355
117	25	-0.33757059354367414
117	26	-0.24642286410769287
117	27	-0.29455628528094024
117	28	-0.28076610246355926
117	29	-0.10049551223320208
117	30	-0.07462980461296809
117	31	-0.024998713253573612
117	32	-0.018206657510248192
117	33	0.05155944959794567
117	34	0.005774745461692987
117	35	-0.09039623386215823
117	36	0.07257180632203131
117	37	0.018621561767353967
117	38	-0.05757215638691804
117	39	0.10790197104041255
117	40	-0.0563156898518784
117	41	0.18483921626229716
117	42	0.20539264784427758
117	43	0.2650435257718087
117	44	0.18035156295884747
117	45	0.3774804587169456
117	46	0.3089279821390818
117	47	0.30255158178714225
117	48	0.35536405934490745
117	49	0.45448906665412636
117	50	0.32384254815263347
118	1	-0.3242681302731185
118	2	-0.3248639499512862
118	3	-0.30895026086549393
118	4	-0.4313153878410958
118	5	-0.2784421555146892
118	6	-0.33003843715151504
118	7	-0.27636645681649696
118	8	-0.3053585015265199
118	9	-0.23799063550579547
118	10	-0.2704094671362256
118	11	-0.26735503600365007
118	12	-0.3950837956786941
118	13	-0.26534834266750096
118	14	-0.4036996361175582
118	15	-0.2722998359927544
118	16	-0.3374985077081547
118	17	-0.25214048256157895
118	18	-0.1891571164144139
118	19	-0.2111301275240649
118	20	-0.39500124603704734
118	21	-0.25955285004010165
118	22	-0.20256214159186925
118	23	-0.31715985839025507
118	24	-0.26971214460658643
118	25	-0.17350233528664916
118	26	-0.06813240581836766
118	27	-0.0743421173651555
118	28	-0.03337781445267041
118	29	-0.22043593062423383
118	30	-0.1739010797258871
118	31	0.0665427451698477
118	32	-0.07790483579348972
118	33	-0.08500438129174626
118	34	0.14090194996815342
118	35	0.10341294553712288
118	36	-0.10745929139156374
118	37	0.18859934929265165
118	38	0.145888453459456
118	39	0.2743356609246135
118	40	0.1670179120016897
118	41	0.32010078297863487
118	42	0.34039188731365044
118	43	-3.418354101043519E-4
118	44	0.4366895048380357
118	45	0.33631339510101554
118	46	0.3854563251962091
118	47	0.06650147367143688
118	48	0.4332512475641792
118	49	0.0876750201493266
118	50	0.2422878716445922
119	1	-0.26384940456991757
119	2	-0.44914552251427864
119	3	-0.5108341235441713
119	4	-0.3797977731284021
119	5	-0.41647665624661556
119	6	-0.5387872137395959
119	7	-0.41616323902820795
119	8	-0.402381615502847
119	9	-0.3848675564577846
119	10	-0.4599314150456024
119	11	-0.38119495533009484
119	12	-0.3398314690224069
119	13	-0.4503291015102775
119	14	-0.5251147185665985
119	15	-0.3393154249496746
119	16	-0.4853293879304701
119	17	-0.43253366489791034
119	18	-0.38756715229256855
119	19	-0.33932476983640214
119	20	-0.3627486961765314
119	21	-0.467334601369501
119	22	-0.36411232032865115
119	23	-0.5419922419746399
119	24	-0.36962544231705863
119	25	-0.3346155549164962
119	26	-0.42058812585683825
119	27	-0.3039792167809523
119	28	-0.33851260567316643
119	29	-0.148391944840796
119	30	-0.34828310923103345
119	31	-0.09887543131647902
119	32	0.012349902038785388
119	33	0.036059691019111995
119	34	-0.10920461368957746
119	35	-0.034657339791252184
119	36	0.05650895369731046
119	37	-0.07039457685876016
119	38	0.03951041241811026
119	39	0.1713666786133956
119	40	-0.07050044814742051
119	41	-0.015489461254269088
119	42	0.17596887840544895
119	43	0.30949947470333683
119	44	0.09841124810773749
119	45	0.27034955912755265
119	46	0.03604263628403004
119	47	0.4678469541714705
119	48	0.18719107086044318
119	49	0.5156329277897953
119	50	0.5853094804154253
120	1	-0.45956645102860705
120	2	-0.4698763889748818
120	3	-0.4919298641919596
120	4	-0.5138107090620352
120	5	-0.39625746306649035
120	6	-0.46477197876163245
120	7	-0.4061821303508921
120	8	-0.4166745297758965
120	9	-0.3953993414039658
120	10	-0.4499022185205206
120	11	-0.3541909816147282
120	12	-0.5502972445203866
120	13	-0.39512222333176084
120	14	-0.498381448963201
120	15	-0.3690614862918622
120	16	-0.3925562294303617
120	17	-0.3915952686170172
120	18	-0.33165415343976434
120	19	-0.334948405229639
120	20	-0.37568732443578057
120	21	-0.4059992720200256
120	22	-0.4202432069681237
120	23	-0.530118902411786
120	24	-0.3821198373906185
120	25	-0.33382178567141096
120	26	-0.32204950411768607
120	27	-0.27585898053090574
120	28	-0.32660041032460896
120	29	-0.1525418495575579
120	30	-0.2889196809520769
120	31	-0.11558683672436842
120	32	-0.24229672629855514
120	33	-0.19090774486225115
120	34	-0.06764458126514228
120	35	-0.048062814022799216
120	36	0.04779329911693253
120	37	-0.06449195015285625
120	38	0.005217374462091584
120	39	0.15242606510342682
120	40	-0.02906733960714547
120	41	0.06615998165903392
120	42	0.2483401750002301
120	43	0.03028083528501213
120	44	0.20441072453393616
120	45	0.24767847206877036
120	46	0.1832144623158527
120	47	0.24943863589398774
120	48	0.3223600042416379
120	49	0.3512594230696138
120	50	0.2880179268300722
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	9782.0
3	1107.0
4	1520.0
5	1834.0
6	2741.0
7	4955.0
8	5945.0
9	7663.0
10	11763.0
11	16873.0
12	19553.0
13	27685.0
14	37002.0
15	43436.0
16	50462.0
17	62631.0
18	75282.0
19	87200.0
20	101237.0
21	112414.0
22	138161.0
23	171765.0
24	207692.0
25	210085.0
26	294216.0
27	350289.0
28	390969.0
29	425320.0
30	537818.0
31	651325.0
32	796289.0
33	969245.0
34	1336993.0
35	1835996.0
36	2464070.0
37	3889016.0
38	6011453.0
39	1.5769799E7
40	19031.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.397672783800004	20.038020312699114	13.15587038533678	22.408436518164102
2	16.73293315374173	22.71818866744546	37.42521936854292	23.123658810269887
3	18.101505555075974	27.41658637863269	32.916855728129626	21.565052338161706
4	11.156142223340552	16.593474061570614	37.732340391386835	34.518043323701995
5	11.53045981211706	41.1874550713683	35.860254845360714	11.421830271153924
6	29.48949946107221	37.901483574283574	18.661294373657373	13.947722590986848
7	26.153660524263007	34.38279493078328	22.889273809118716	16.574270735835007
8	29.346430504774656	34.246532163920726	20.402818612676068	16.00421871862855
9	27.351198500956258	12.416235240453744	20.515858458016996	39.716707800573005
10	16.661612914800312	28.469909396121203	35.47475671803782	19.393720971040672
11	34.29478439079491	22.008102853311964	23.978056676689917	19.719056079203206
12	20.06256585186728	28.003755630761123	33.14046708833934	18.793211429032255
13	29.381877738972623	20.85875791438208	27.596542749567483	22.162821597077816
14	19.984922273017276	20.92709851440217	31.551655144868544	27.536324067712002
15	23.399283429471396	29.441151164188216	25.76895936845106	21.390606037889327
16	21.259224986344098	27.5002552448888	28.420794960576274	22.819724808190838
17	19.549319436284698	28.392467444471713	30.011451251053618	22.046761868189968
18	19.59055384064857	27.26138002522128	31.705951102140844	21.44211503198931
19	20.68615033321799	26.995102771543024	32.70452526950772	19.61422162573126
20	21.86439256813056	25.52536981624361	32.75474693574482	19.855490679881008
21	22.286747228264094	25.501561151808232	32.083878918845	20.127812701082664
22	20.838986560838695	25.352915498796563	32.640400734232955	21.167697206131784
23	20.714466100332157	26.462652624533916	32.40499006177093	20.417891213362992
24	21.04155450235844	25.841388773073813	32.577941795072	20.53911492949574
25	20.403729173319313	26.59779058429686	32.64641728292727	20.352062959456557
26	20.308661522281042	25.66418046724126	32.56412411214342	21.46303389833428
27	20.190788617447396	25.868990900811212	32.38380119092818	21.556419290813206
28	21.142597285571153	25.805862719690754	32.63824162662232	20.41329836811578
29	20.15185750203146	26.80967584383079	32.592640857451954	20.445825796685796
30	20.206897001088244	26.143388721228728	33.11471277555353	20.535001502129504
31	20.508948043630657	26.624518206344266	32.357599169488644	20.50893458053643
32	20.314260035022176	26.05494920115165	33.25201605005891	20.378774713767264
33	20.377008063491107	26.225039908141262	32.19168941233242	21.20626261603521
34	21.495248520406886	25.812766904478696	32.22130408922842	20.470680485885996
35	20.608434693644337	26.13147043505987	32.93028370760409	20.3298111636917
36	20.771141270355916	27.33958249424066	31.510586586579905	20.378689648823524
37	20.73263349119334	26.194268283714866	32.60026436006628	20.47283386502552
38	21.624666994210106	26.68405785174088	31.275552077845177	20.415723076203832
39	20.67529558453804	26.75590949558948	31.258698871563407	21.31009604830907
40	20.707046206794402	26.418873571090323	31.369836842141694	21.50424337997358
41	21.616099898288514	26.64549726750551	30.94483507485845	20.79356775934752
42	20.556346188192876	26.91298678971276	31.669364840306624	20.861302181787742
43	20.865894632439712	26.677199956792947	30.813068355225536	21.643837055541802
44	20.8462362382671	26.96540351662321	31.37791615709009	20.810444088019594
45	20.82911814677757	27.077505203048634	31.33773787434103	20.755638775832765
46	21.057407992077486	27.17279627257531	29.91804914605618	21.851746589291025
47	21.174988529975224	27.36364545001696	30.64175293768685	20.819613082320966
48	22.228154522869495	27.70828120046143	28.943271614029403	21.120292662639674
49	21.392240495044803	27.671717952541197	29.090461922869892	21.84557962954411
50	21.38793300962432	27.680696479429358	29.80086272046258	21.130507790483744
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	11839.0
1	12218.0
2	12597.0
3	33393.5
4	54190.0
5	64636.5
6	75083.0
7	61637.5
8	48192.0
9	62724.5
10	77257.0
11	114114.5
12	150972.0
13	261867.5
14	372763.0
15	387735.5
16	402708.0
17	515076.0
18	627444.0
19	597871.0
20	568298.0
21	590777.0
22	613256.0
23	657569.0
24	701882.0
25	761397.0
26	820912.0
27	909659.0
28	998406.0
29	1092926.0
30	1187446.0
31	1306774.0
32	1426102.0
33	1547150.5
34	1668199.0
35	1774127.5
36	1880056.0
37	1959664.5
38	2039273.0
39	2086477.5
40	2133682.0
41	2167593.5
42	2201505.0
43	2231373.5
44	2261242.0
45	2233652.5
46	2206063.0
47	2147107.5
48	2088152.0
49	2027500.5
50	1966849.0
51	1896051.5
52	1825254.0
53	1755204.5
54	1685155.0
55	1585374.5
56	1485594.0
57	1474311.0
58	1463028.0
59	1341739.0
60	1220450.0
61	1042496.5
62	864543.0
63	747427.5
64	630312.0
65	541440.5
66	452569.0
67	384584.0
68	316599.0
69	270782.0
70	224965.0
71	192436.5
72	159908.0
73	130185.0
74	100462.0
75	78945.5
76	57429.0
77	44490.5
78	31552.0
79	24543.0
80	17534.0
81	13686.0
82	9838.0
83	7586.5
84	5335.0
85	4149.5
86	2964.0
87	2258.5
88	1553.0
89	1121.0
90	689.0
91	494.0
92	299.0
93	217.0
94	135.0
95	95.0
96	55.0
97	36.5
98	18.0
99	13.5
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.02535085756449213
2	0.0070765984855648565
3	0.0
4	1.6150472009657335E-5
5	0.0038276618662887885
6	0.0
7	0.012600059912867665
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	1.4535424808691602E-4
14	0.0012247441273990148
15	1.3997075741703025E-4
16	5.3834906698857786E-5
17	2.4494882547980293E-4
18	6.406353897164077E-4
19	3.230094401931467E-5
20	1.9380566411588803E-4
21	4.3606274426074806E-4
22	2.6917453349428893E-5
23	3.633856202172901E-4
24	0.002357968913409971
25	0.0016527316356549342
26	0.0012731955434279866
27	0.0016204306916356194
28	0.0014939186608933035
29	0.026586368673230918
30	0.026947062548113267
31	0.03244091477673171
32	0.015025322459651208
33	0.04128329820201909
34	0.0237358103635264
35	0.05638129778571376
36	0.059807889597096056
37	0.07269596626080262
38	0.04745277850970819
39	0.06372707080477291
40	0.06640535741304109
41	0.06733670129893132
42	0.09359198529596426
43	0.07280363607420033
44	0.08276040206815408
45	0.09905353658056339
46	0.06190745095835151
47	0.0864777023757102
48	0.08054778740283101
49	0.09754615919299536
50	0.10357836048860239
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	3.7150617E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.01909248192507
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.53908291960627	25.407537512714796
2	12.499706696227786	9.004561829767335
3	5.501991252740987	5.945301953016613
4	2.977645104570576	4.290082975995162
5	1.8564521543504247	3.3433860917908493
6	1.2738237573624034	2.7529185433265817
7	0.9238229315371425	2.3292684525571627
8	0.6761486046979271	1.948340729931139
9	0.5003607516961174	1.62202861707012
>10	2.8528788610806566	18.684078226096346
>50	0.2338216322042771	5.846747947032188
>100	0.14865651759312504	10.125215267943206
>500	0.010794676643462415	2.6335716782138596
>1k	0.004454543783790041	2.8143767724421798
>5k	2.0227269898457888E-4	0.5071102427617611
>10k+	1.5732320624381324E-4	2.745473159340183
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	195230	0.5255094417409003	No Hit
TCTCTGAGCGGGCTGGCAAGGCAGACCGATATCGTATGCCGTCTTCTGCT	175472	0.4723259374130987	Illumina PCR Primer Index 1 (95% over 24bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	156828	0.42214103738842346	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	97460	0.262337500343534	No Hit
TCTCTGAGCGGGCTGGCAAGGCAGACCGATCTCGTATGCCGTCTTCTGCT	92349	0.2485799899366409	Illumina Paired End PCR Primer 2 (96% over 29bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	44409	0.11953771857947879	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	2.6917453349428895E-6	0.0	0.0	0.0
4	0.0	5.383490669885779E-6	0.0	0.0	0.0
5	0.0	5.383490669885779E-6	0.0	0.0	0.0
6	2.6917453349428895E-6	5.383490669885779E-6	0.0	2.6917453349428895E-6	0.0
7	5.383490669885779E-6	5.383490669885779E-6	0.0	1.0766981339771558E-5	0.0
8	1.3458726674714446E-5	5.383490669885779E-6	0.0	1.0766981339771558E-5	0.0
9	1.3458726674714446E-5	5.383490669885779E-6	0.0	1.8842217344600227E-5	0.0
10	3.7684434689200454E-5	5.383490669885779E-6	0.0	2.4225708014486004E-5	0.0
11	4.306792535908623E-5	5.383490669885779E-6	0.0	3.499268935425756E-5	0.0
12	4.306792535908623E-5	5.383490669885779E-6	0.0	3.7684434689200454E-5	0.0
13	1.022863227278298E-4	5.383490669885779E-6	0.0	4.306792535908623E-5	0.0
14	1.6957995610140204E-4	8.075236004828668E-6	0.0	4.845141602897201E-5	0.0
15	2.1264788146048826E-4	2.4225708014486004E-5	0.0	5.3834906698857786E-5	0.0
16	2.3687358947497425E-4	2.4225708014486004E-5	0.0	5.652665203380068E-5	0.0
17	2.637910428244032E-4	2.6917453349428893E-5	0.0	6.998537870851512E-5	0.0
18	2.6917453349428896E-4	2.960919868437178E-5	0.0	7.267712404345801E-5	0.0
19	2.6917453349428896E-4	2.960919868437178E-5	0.0	7.80606147133438E-5	0.0
20	2.9070849617383204E-4	2.960919868437178E-5	0.0	1.022863227278298E-4	0.0
21	2.960919868437178E-4	2.960919868437178E-5	0.0	1.1843679473748713E-4	0.0
22	2.960919868437178E-4	2.960919868437178E-5	0.0	1.3997075741703025E-4	0.0
23	2.9878373217866075E-4	3.499268935425756E-5	0.0	1.6957995610140204E-4	2.6917453349428895E-6
24	3.068589681834894E-4	3.499268935425756E-5	0.0	2.1803137213037403E-4	2.6917453349428895E-6
25	3.2031769485820383E-4	4.037618002414334E-5	0.0	2.261066081352027E-4	2.6917453349428895E-6
26	3.526186388775185E-4	4.037618002414334E-5	0.0	2.6109929748946025E-4	2.6917453349428895E-6
27	3.687691108871758E-4	4.037618002414334E-5	0.0	3.364681668678612E-4	2.6917453349428895E-6
28	3.822278375618903E-4	4.037618002414334E-5	0.0	4.7643892428489144E-4	2.6917453349428895E-6
29	3.849195828968332E-4	4.037618002414334E-5	0.0	6.837033150754939E-4	2.6917453349428895E-6
30	3.849195828968332E-4	4.037618002414334E-5	0.0	0.0010282467179481838	2.6917453349428895E-6
31	3.849195828968332E-4	4.037618002414334E-5	0.0	0.0016850325796742486	2.6917453349428895E-6
32	3.849195828968332E-4	4.306792535908623E-5	0.0	0.0027940316576707193	2.6917453349428895E-6
33	3.849195828968332E-4	4.306792535908623E-5	0.0	0.004627110230766827	2.6917453349428895E-6
34	3.849195828968332E-4	4.845141602897201E-5	0.0	0.007208494006977058	2.6917453349428895E-6
35	3.849195828968332E-4	5.652665203380068E-5	0.0	0.011264954226735992	2.6917453349428895E-6
36	3.849195828968332E-4	5.652665203380068E-5	0.0	0.01816658926552956	2.6917453349428895E-6
37	3.849195828968332E-4	8.613585071817246E-5	0.0	0.029555363777672924	2.6917453349428895E-6
38	3.849195828968332E-4	8.613585071817246E-5	0.0	0.04549318790586977	2.6917453349428895E-6
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	88130	0.0	36.072403	1
TATGCCG	40405	0.0	35.702324	35
GACCGAT	42545	0.0	34.987373	24
TCGTATG	41765	0.0	34.77973	32
CGTATGC	41965	0.0	34.53661	33
GCGGGCT	47400	0.0	34.37236	8
AGACCGA	45705	0.0	34.362362	23
ACCGATA	27470	0.0	34.31412	25
TATCGTA	26570	0.0	34.264717	30
GCCGTCT	40125	0.0	34.256058	38
CCGTCTT	40425	0.0	33.982754	39
CCGATAT	27550	0.0	33.92881	26
ATGCCGT	41055	0.0	33.90156	36
ATATCGT	26960	0.0	33.748497	29
CGGGCTG	48375	0.0	33.74327	9
GATATCG	27785	0.0	33.675602	28
CGATATC	27870	0.0	33.604427	27
CAGACCG	47195	0.0	33.58729	22
TGAGCGG	48825	0.0	33.514206	5
ATCGTAT	27850	0.0	33.387554	31
>>END_MODULE