FastQCFastQC Report
Thu 26 May 2016
SRR522110_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR522110_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32856475
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1514930.4610750240249448No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1360690.41413146115035165No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT780010.23739917322232526No Hit
TCTCTGAGCGGGCTGGCAAGGCAGACCGATATCGTATGCCGTCTTCTGCT419050.1275395489017005Illumina PCR Primer Index 1 (95% over 24bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT391340.1191058992177341No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA669400.035.5106541
TATGCCG98800.033.09816735
TCGTATG107100.030.47362932
GACCGAT110150.030.3155924
CGTATGC111400.029.35318233
CCGTCTT110000.028.78756139
GCCGTCT110300.028.45163738
GCGGGCT130450.028.1190838
ATATCGT74450.028.08106429
TATCGTA74800.028.00846730
ATGCCGT114250.027.81282636
AGACCGA127850.027.64954423
ACCGATA77750.027.5031425
CGATATC77950.027.46719627
ATCGTAT78200.027.24043331
GATATCG79900.026.90700528
CGGGCTG138100.026.6410569
TGAGCGG139850.026.5921925
CCGATAT80750.026.46019226
TGCCGTC120400.026.3014937
Produced by FastQC (version 0.11.5)