FastQCFastQC Report
Thu 26 May 2016
SRR522109_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR522109_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35319012
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1561170.44201972580659954No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1260080.3567710217941544No Hit
TCTCTGAGCGGGCTGGCAAGGCAGACCGATATCGTATGCCGTCTTCTGCT1045330.29596807521116386Illumina PCR Primer Index 1 (95% over 24bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT731710.20717170684162967No Hit
TCTCTGAGCGGGCTGGCAAGGCAGACCGATCTCGTATGCCGTCTTCTGCT587610.16637215106696643Illumina Paired End PCR Primer 2 (96% over 29bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA811900.035.550131
TATGCCG272550.032.73412735
CCGTCTT267500.031.34463339
ATGCCGT274750.031.15034536
GCCGTCT269800.031.12852738
TGCCGTC283700.030.14482937
TCGTATG297250.030.10986332
GACCGAT304350.030.09748824
CGTCTTC279450.029.79859440
CGTATGC302900.029.50972233
TGAGCGG349750.029.5037255
TATCGTA181100.029.29565630
GCGGGCT350400.029.2714758
ACCGATA189100.029.24658825
AGACCGA335700.029.16728623
ATATCGT182550.029.02679429
CGATATC188950.028.96402427
CCGATAT189600.028.85426526
CAGACCG345600.028.71367822
AGCGGGC359650.028.5493227
Produced by FastQC (version 0.11.5)