##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR522109_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	35319012
Sequences flagged as poor quality	0
Sequence length	50
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.276494653927465	39.0	38.0	40.0	35.0	40.0
2	38.07119069468874	39.0	38.0	40.0	35.0	40.0
3	37.95926233723639	39.0	38.0	40.0	34.0	40.0
4	38.0527740979844	39.0	38.0	40.0	35.0	40.0
5	38.09641495634136	39.0	38.0	40.0	35.0	40.0
6	38.13863615437487	39.0	38.0	40.0	35.0	40.0
7	38.070050855329704	39.0	38.0	40.0	35.0	40.0
8	37.99917911067274	39.0	38.0	40.0	35.0	40.0
9	38.01282530213473	39.0	38.0	40.0	35.0	40.0
10	37.90575781111884	39.0	38.0	40.0	34.0	40.0
11	37.9689533501107	39.0	38.0	40.0	35.0	40.0
12	37.80632266836909	39.0	38.0	40.0	33.0	40.0
13	37.79881382299143	39.0	38.0	40.0	33.0	40.0
14	37.70662602340065	39.0	38.0	40.0	33.0	40.0
15	37.67925110702417	39.0	38.0	40.0	33.0	40.0
16	37.52794325050769	39.0	38.0	40.0	33.0	40.0
17	37.473692157640194	39.0	37.0	40.0	33.0	40.0
18	37.39087817632045	39.0	37.0	40.0	33.0	40.0
19	37.610819436285475	39.0	38.0	40.0	33.0	40.0
20	37.54325503216228	39.0	38.0	40.0	33.0	40.0
21	37.58919785185384	39.0	38.0	40.0	33.0	40.0
22	37.46434410452931	39.0	38.0	40.0	33.0	40.0
23	37.38614038807201	39.0	37.0	40.0	33.0	40.0
24	37.40185487068551	39.0	38.0	40.0	33.0	40.0
25	37.33046422702878	39.0	37.0	40.0	33.0	40.0
26	37.22878046531992	39.0	37.0	40.0	33.0	40.0
27	37.09954137448692	39.0	37.0	40.0	33.0	40.0
28	36.98561539603656	39.0	37.0	40.0	32.0	40.0
29	36.8958661131291	39.0	36.0	40.0	32.0	40.0
30	36.749450381001594	39.0	36.0	40.0	31.0	40.0
31	36.46020010412522	39.0	36.0	40.0	31.0	40.0
32	36.34260026865984	39.0	36.0	40.0	30.0	40.0
33	36.18603320500585	39.0	36.0	40.0	30.0	40.0
34	36.04152706763145	39.0	36.0	40.0	30.0	40.0
35	35.88086515557117	39.0	36.0	40.0	29.0	40.0
36	35.64327781875665	39.0	35.0	40.0	29.0	40.0
37	35.453929770175904	39.0	35.0	40.0	29.0	40.0
38	35.32152196103334	39.0	35.0	40.0	28.0	40.0
39	35.10991060565341	39.0	35.0	40.0	28.0	40.0
40	34.87285315342343	38.0	35.0	40.0	27.0	40.0
41	34.45482628449516	38.0	35.0	40.0	25.0	40.0
42	34.18563109862756	38.0	34.0	40.0	23.0	40.0
43	33.95909245705967	38.0	34.0	40.0	23.0	40.0
44	33.64196671186612	38.0	33.0	40.0	20.0	40.0
45	33.2587355784471	38.0	33.0	40.0	15.0	40.0
46	32.5860175533789	38.0	33.0	40.0	2.0	40.0
47	32.37173480390675	38.0	33.0	40.0	2.0	40.0
48	32.10795435047844	38.0	32.0	39.0	2.0	40.0
49	31.91003434637413	38.0	32.0	39.0	2.0	40.0
50	31.640225609934955	37.0	32.0	39.0	2.0	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
2	1	-0.41478895826572426
2	2	-0.48441523748062565
2	3	-0.4981401977113791
2	4	-0.5280241896237428
2	5	-0.4918369059874408
2	6	-0.44526035867203717
2	7	-0.5696211426829834
2	8	-0.43844697369995345
2	9	-0.6345030466422301
2	10	-0.6975169300700443
2	11	-0.5100001769174511
2	12	-0.6422008178257883
2	13	-0.6904529900802387
2	14	-0.6539590405440947
2	15	-0.6610970629105779
2	16	-0.7895306462473997
2	17	-0.6345787120702084
2	18	-0.7487714103420657
2	19	-0.9161397925797345
2	20	-0.9394087897156496
2	21	-0.983498553715485
2	22	-1.0159744705377278
2	23	-0.9469105236797617
2	24	-0.9537549436198205
2	25	-0.8565230963822827
2	26	-1.2129079162363965
2	27	-1.429001897605204
2	28	-1.083115160546896
2	29	-1.2780501213001045
2	30	-1.1122448017760505
2	31	-1.1337532241150754
2	32	-1.0392262841130488
2	33	-1.0881955676571735
2	34	-1.2064891712297268
2	35	-1.228177734925744
2	36	-1.248225063638202
2	37	-1.0936036133073728
2	38	-1.1458910471322739
2	39	-1.1604648204769603
2	40	-1.19446266905031
2	41	-1.4975673594972179
2	42	-1.1548722077814801
2	43	-1.1599864070271124
2	44	-1.2949711634793744
2	45	-1.1733452666548772
2	46	-1.3656324081465634
2	47	-1.1656516568665332
2	48	-1.2825128781173554
2	49	-1.3080318740383916
2	50	-1.327632880539543
3	1	-0.20288990869217116
3	2	-0.30891079521484244
3	3	-0.38575531817831177
3	4	-0.3684302873891454
3	5	-0.3280066264472623
3	6	-0.2574782760646883
3	7	-0.34079103585525417
3	8	-0.32930070149497226
3	9	-0.2993088365730898
3	10	-0.28522128699555793
3	11	-0.1622406637633702
3	12	-0.22217797914429838
3	13	-0.20433970087324127
3	14	-0.26082916586221216
3	15	-0.2746467050402259
3	16	-0.2722396562494822
3	17	-0.24782184040063981
3	18	-0.22241498264508408
3	19	-0.38931845344868066
3	20	-0.24374433444562982
3	21	-0.26759596861651147
3	22	-0.30924612690091635
3	23	-0.265006846428534
3	24	-0.1791139264450905
3	25	-0.25521314865319766
3	26	-0.03719697083892726
3	27	-0.1206106327913048
3	28	-0.02435744055441802
3	29	-0.017602389674934216
3	30	-0.11801982349209794
3	31	0.037110510842431665
3	32	0.09091553820648102
3	33	0.08179653328816983
3	34	0.06193902864937684
3	35	0.1840265534227683
3	36	0.17786968945919313
3	37	0.12805608221074039
3	38	0.12789811668547912
3	39	0.2899371626683376
3	40	0.3170124324182524
3	41	0.29191465554908547
3	42	0.3436426023110215
3	43	0.3124301652403503
3	44	0.4952046374746075
3	45	0.3203639566978822
3	46	0.5858156587439538
3	47	0.5565653106076454
3	48	0.39659269998676905
3	49	0.5167570713489127
3	50	0.525250188052695
4	1	-0.15518788566459563
4	2	-0.2850993802324169
4	3	-0.33749321051572423
4	4	-0.30206158554008766
4	5	-0.2470948913506632
4	6	-0.2667132287971512
4	7	-0.21644229528828163
4	8	-0.20886990638170744
4	9	-0.2394830269548649
4	10	-0.305542513063223
4	11	-0.19462345260380687
4	12	-0.19975707206641857
4	13	-0.24540836546921696
4	14	-0.23232112732420518
4	15	-0.21307117556191457
4	16	-0.22683807174014703
4	17	-0.1753918269696939
4	18	-0.15511427435235703
4	19	-0.24996954851837927
4	20	-0.3448669145079464
4	21	-0.1914268417184175
4	22	-0.21141759876788768
4	23	-0.2882940380433041
4	24	-0.075521623396277
4	25	-0.09512663380931485
4	26	-0.16098491817363936
4	27	0.032107440842374046
4	28	-0.14288403379921277
4	29	-0.11071150592103152
4	30	0.04288514264631971
4	31	0.011105858540368274
4	32	0.08931674956030378
4	33	-0.09241862596115169
4	34	0.050651974381445086
4	35	0.1389790703797118
4	36	0.0811193964747261
4	37	0.1571968219612785
4	38	0.1365339787492772
4	39	0.039948621251937766
4	40	0.1397909039142533
4	41	0.11895913820008275
4	42	0.13392615091606785
4	43	0.21660656714827553
4	44	0.18838853708320613
4	45	0.13081352124437728
4	46	0.0022439237551097335
4	47	0.15369175160749649
4	48	0.22275023946539818
4	49	0.20636298235394435
4	50	0.33099080179395557
5	1	-0.19485966496124973
5	2	-0.30376356531567694
5	3	-0.28570394572901137
5	4	-0.31150671836170574
5	5	-0.3151801441109825
5	6	-0.28383324948635646
5	7	-0.3711272999733026
5	8	-0.4003823291862503
5	9	-0.7336790108679239
5	10	-0.35451414884997945
5	11	-0.2802515165966071
5	12	-0.3387345059306952
5	13	-0.4614227241929001
5	14	-0.3248305956749391
5	15	-0.3281188320636801
5	16	-0.25546701810024075
5	17	-0.246336687396024
5	18	-0.24022686861307108
5	19	-0.35289765422557196
5	20	-0.45145407374113233
5	21	-0.26754293055223854
5	22	-0.36557497545692286
5	23	-0.49732370988193964
5	24	-0.33494526191250173
5	25	-0.40150629200526566
5	26	-0.33834639341992556
5	27	-0.23083544197810113
5	28	-0.23793166192737658
5	29	-0.49820748928219416
5	30	-0.3006691297416637
5	31	-0.058739814379791255
5	32	-0.5271329697338842
5	33	-0.2069420432964577
5	34	-0.32738824254308696
5	35	-0.0673860450447279
5	36	0.06249176403758128
5	37	-0.15650974516039184
5	38	-0.09186459684025294
5	39	-0.2966715458295113
5	40	-0.13534300005039057
5	41	0.06882142715472384
5	42	-0.013092534211850193
5	43	0.2555797350233391
5	44	0.15994287082046554
5	45	-0.04904757368311152
5	46	-0.19562696613967034
5	47	-0.056900453941018725
5	48	-0.3078730579343194
5	49	-0.30574628517047486
5	50	0.027736340775113888
6	1	-0.11718382919956838
6	2	-0.32858452988900666
6	3	-0.2503526101750495
6	4	-0.2799480285525888
6	5	-0.22466465646993328
6	6	-0.24566738776373853
6	7	-0.3017001583001715
6	8	-0.27296155627197294
6	9	-0.2820610417642797
6	10	-0.3298842538242397
6	11	-0.2436007972595391
6	12	-0.19589221637860987
6	13	-0.35707078201594555
6	14	-0.24269549012394975
6	15	-0.24350537188833954
6	16	-0.18920357078199146
6	17	-0.1408668383793099
6	18	-0.20943768043144928
6	19	-0.2806672940491737
6	20	-0.2590497064625268
6	21	-0.2179191236674427
6	22	-0.22259975236463703
6	23	-0.2342570504250503
6	24	-0.1740485890893524
6	25	-0.11725647565374686
6	26	-0.2623588299215811
6	27	-0.04657530656678688
6	28	-0.1868467207796698
6	29	-0.1914456762411305
6	30	-0.26818332875229345
6	31	-0.08919648937943947
6	32	-0.015407755445146165
6	33	-0.05491181914592147
6	34	-0.012133009885069157
6	35	-0.08885446290488375
6	36	0.05523856837657348
6	37	0.004754028997645321
6	38	0.03876109710738973
6	39	-0.10845561866850773
6	40	0.035898941053446265
6	41	0.16613273691208974
6	42	0.2014602759242834
6	43	0.14898530151442202
6	44	0.23126552022289104
6	45	0.11402521173736346
6	46	0.15528224188918216
6	47	0.15812217860555933
6	48	0.24713100004377253
6	49	0.04187803302425408
6	50	0.10307551335032983
7	1	-0.11911930957824524
7	2	-0.21049046276468175
7	3	-0.23824402427530345
7	4	-0.18441762047132926
7	5	-0.19812429008042898
7	6	-0.12749210739869454
7	7	-0.09146318264641451
7	8	-0.06495892354976007
7	9	-0.20720534519257683
7	10	-0.09505885249397039
7	11	-0.25701826494148605
7	12	-0.27976801124357564
7	13	-0.12168121262170217
7	14	-0.21717511733656636
7	15	-0.17910176449083082
7	16	-0.17099682098947255
7	17	-0.20319625157654286
7	18	-0.07100433446378673
7	19	-0.2775125887139893
7	20	-0.20434192279648755
7	21	-0.20668766172123298
7	22	-0.33852018034574627
7	23	-0.1699452631338545
7	24	-0.06390245570026565
7	25	-0.20709610147565627
7	26	-0.030782018365165698
7	27	-0.13948635627047423
7	28	-0.14477729976591291
7	29	-0.12156716452852834
7	30	0.00967207167308004
7	31	0.05754356313841669
7	32	0.054953486031912746
7	33	0.22360246029111153
7	34	0.2198509097419361
7	35	0.19716698232644347
7	36	0.25535349193186363
7	37	0.25831136644971053
7	38	0.09582280946952437
7	39	0.27698711892489314
7	40	0.3404819567822628
7	41	0.26213389425751643
7	42	0.28420752318364606
7	43	0.4440154213652079
7	44	0.2811946325242829
7	45	0.41999801400334746
7	46	0.08860815604306538
7	47	0.4155394101309824
7	48	0.3102173034158824
7	49	0.3739987515329801
7	50	0.2454125680695718
8	1	-0.1214343641234521
8	2	-0.21976382697162933
8	3	-0.1750558984696866
8	4	-0.1674597906703994
8	5	-0.1861330814082507
8	6	-0.18462818157560434
8	7	-0.24480780093796994
8	8	-0.17811272619211138
8	9	-0.16030229512935534
8	10	-0.19861568961563592
8	11	-0.0274891542084319
8	12	-0.07209710441773609
8	13	-0.1062322287694073
8	14	-0.04451525270977896
8	15	-0.08423741697655629
8	16	-0.07688221742084522
8	17	0.010538009065953702
8	18	-0.1459174387682438
8	19	-0.3239475430408092
8	20	-0.08573306706548323
8	21	-0.12002568616975395
8	22	-0.05246887396711486
8	23	-0.1092323550631562
8	24	-0.08110280808165982
8	25	-0.06447407333390487
8	26	-0.02383935818346572
8	27	0.157111773877034
8	28	0.22473406460336065
8	29	0.19265907302634133
8	30	0.05191407628159794
8	31	0.27866596760831186
8	32	0.26534638017276535
8	33	0.16832579275935444
8	34	0.2555922545804066
8	35	0.21765350950121842
8	36	0.1880779703571065
8	37	0.26654823901627367
8	38	0.2977434022256986
8	39	0.23722530809991582
8	40	0.24841596157099843
8	41	0.24590864682060243
8	42	0.4788105355677601
8	43	0.3131300210764323
8	44	0.1844899455030813
8	45	0.28260239530229114
8	46	0.49388235098012245
8	47	0.21421709271493938
8	48	0.5443466630902307
8	49	0.3328815499269453
8	50	0.32216132899489125
9	1	-0.07547315143972355
9	2	-0.16464524283487947
9	3	-0.18385735467258257
9	4	-0.1964417083745431
9	5	-0.17153296827203235
9	6	-0.1201597727753807
9	7	-0.2264896624191195
9	8	-0.2856966065236435
9	9	-0.1693645338207972
9	10	-0.2297236213986409
9	11	-0.23728880476976855
9	12	-0.18719765783129816
9	13	-0.08657392949730536
9	14	-0.1524553195953331
9	15	-0.23176881259976057
9	16	-0.07806449943455362
9	17	-0.08937436130720045
9	18	-0.11804449925571703
9	19	-0.18485434207422458
9	20	-0.09633351548347946
9	21	-0.02386493780568344
9	22	-0.08024912053585354
9	23	-0.10285345577464966
9	24	-0.017365460722650994
9	25	-0.1154356403232839
9	26	-0.06789036130788162
9	27	-0.06640545547961807
9	28	0.06675640458352916
9	29	0.10156178348884737
9	30	0.12154224433130878
9	31	0.16597475295289854
9	32	-0.11659806552327723
9	33	0.042938523693671016
9	34	0.16273829431936804
9	35	0.16615037294624813
9	36	0.005614907789144752
9	37	0.03610000884552278
9	38	0.11289442890236501
9	39	0.041159185743907756
9	40	0.1583512460609029
9	41	-0.024630007733144055
9	42	0.0585440674697324
9	43	-0.004379705436925008
9	44	0.1665237379975295
9	45	0.09821679178948273
9	46	0.08739761498445375
9	47	0.06885122854937009
9	48	0.12765297883687055
9	49	-0.028410537078464415
9	50	0.19199936815737928
10	1	-0.12715277037956696
10	2	-0.1900762242072318
10	3	-0.1517852972860112
10	4	-0.1500557781333285
10	5	-0.14877820562147548
10	6	-0.14454785166449113
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115	40	0.44094490585776924
115	41	0.49470223214417786
115	42	0.5241298397421303
115	43	0.3928182790450805
115	44	0.303801710085267
115	45	0.39900933593659005
115	46	0.4466631415097595
115	47	0.5936020656924654
115	48	0.45180008633131763
115	49	0.5656935719407485
115	50	0.5129903844087735
116	1	-0.22331182183663145
116	2	-0.3064684064074896
116	3	-0.27899526280437215
116	4	-0.308533428809028
116	5	-0.2778435827894157
116	6	-0.2839491137185064
116	7	-0.22536997034953288
116	8	-0.3330302696933458
116	9	-0.25282002636509304
116	10	-0.33585063378578184
116	11	-0.24783980026310104
116	12	-0.2722579284611868
116	13	-0.19036267595612344
116	14	-0.2133135773400241
116	15	-0.22945337193795012
116	16	-0.10318384780692469
116	17	-0.10902514428588717
116	18	-0.12675348667720954
116	19	-0.10150730259580598
116	20	-0.1584449050993939
116	21	-0.031827909550152356
116	22	-0.03714960851364424
116	23	-0.04455028038291431
116	24	-0.011668948612417296
116	25	0.02146294745013222
116	26	0.1731021914588382
116	27	0.207684839472968
116	28	0.1553564394877185
116	29	0.13567064475418533
116	30	0.29701329359217254
116	31	0.21953188197456086
116	32	0.3293810980603382
116	33	0.4355730885688587
116	34	0.3864864026253585
116	35	0.430115607440257
116	36	0.5052083693206413
116	37	0.4319262139651556
116	38	0.5175477156632127
116	39	0.4890430843894009
116	40	0.611015113584898
116	41	0.7083006036814297
116	42	0.7105317446888932
116	43	0.738848478878829
116	44	0.6292657784332434
116	45	0.8489572168248358
116	46	0.952738998695736
116	47	0.9434811151816547
116	48	1.0295366888179203
116	49	0.9140641180702573
116	50	0.938512744959958
117	1	-0.35770460328292586
117	2	-0.4055925388054433
117	3	-0.38199576023632176
117	4	-0.3648252578414173
117	5	-0.31750007528663815
117	6	-0.3318188350362945
117	7	-0.29411005184646655
117	8	-0.2557788244535928
117	9	-0.32794951221026736
117	10	-0.22973581965587186
117	11	-0.24984194004683502
117	12	-0.3114433906717693
117	13	-0.18944361941630916
117	14	-0.2836435932041823
117	15	-0.20305123599224828
117	16	-0.2054821963034783
117	17	-0.11074417176494933
117	18	-0.15476492880778636
117	19	-0.2765418377794049
117	20	-0.1892817060259233
117	21	-0.13251552735218297
117	22	-0.10606703673111184
117	23	-0.23042245084328528
117	24	-0.19755426315375502
117	25	-0.066116147265177
117	26	-0.11045169985579406
117	27	-0.03639276643491485
117	28	0.09989588277103678
117	29	-0.1211709515447339
117	30	0.08669819493566422
117	31	0.2105237521826595
117	32	0.114972571639548
117	33	0.24591469445531544
117	34	0.1252423678020378
117	35	0.22219758582466653
117	36	0.3415916667033656
117	37	0.30573823535545586
117	38	0.31445695993848943
117	39	0.4537824091898415
117	40	0.4353591932978702
117	41	0.6684884083079936
117	42	0.6398410270933539
117	43	0.63273405067185
117	44	0.8285899169030557
117	45	0.5903427643652535
117	46	0.8245266840584904
117	47	0.9602951983538546
117	48	0.884758795106876
117	49	0.7983965914916311
117	50	0.8920926901585986
118	1	-0.2624689806700289
118	2	-0.3495713767581279
118	3	-0.3695932477122099
118	4	-0.35349883499461043
118	5	-0.29701477431920864
118	6	-0.30367838535619285
118	7	-0.2974846715758517
118	8	-0.2963669671035589
118	9	-0.3039000927086448
118	10	-0.47638900499566716
118	11	-0.26218864143209686
118	12	-0.3186525192747496
118	13	-0.4686964505306861
118	14	-0.41541215104435025
118	15	-0.26881364160911403
118	16	-0.3990484472296387
118	17	-0.38292028893913255
118	18	-0.3515789011476187
118	19	-0.33861155154218636
118	20	-0.3125569430680457
118	21	-0.4773318182551094
118	22	-0.2982899789817921
118	23	-0.2598183049801719
118	24	-0.28391861702827015
118	25	-0.19879383036725073
118	26	-0.22729621623161478
118	27	-0.20657160417319886
118	28	-0.3813170471533951
118	29	-0.25070085514846596
118	30	-0.8722397032351594
118	31	-0.3374249171136299
118	32	-0.15837365877920462
118	33	-0.40369730311267205
118	34	-0.1549479296447558
118	35	-0.33537894042573413
118	36	-0.18724321737116156
118	37	0.0030223255316030873
118	38	0.017053671999107678
118	39	-0.1510837950506101
118	40	-0.16095761100500994
118	41	0.030268873417789166
118	42	-0.08494973585273158
118	43	0.0011581183109328208
118	44	-0.21177692419631455
118	45	0.2455454791762719
118	46	0.3574888454016829
118	47	0.1479175071581622
118	48	0.38648530006909
118	49	0.38072119264857207
118	50	0.17616122525444666
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	18415.0
3	2347.0
4	2848.0
5	3379.0
6	4870.0
7	8823.0
8	9945.0
9	12781.0
10	19106.0
11	25587.0
12	29343.0
13	39660.0
14	50621.0
15	58059.0
16	65811.0
17	78482.0
18	92429.0
19	106377.0
20	123177.0
21	137250.0
22	164274.0
23	202077.0
24	241182.0
25	256745.0
26	339712.0
27	400489.0
28	449713.0
29	501617.0
30	619896.0
31	742013.0
32	897133.0
33	1081255.0
34	1440042.0
35	1921680.0
36	2533093.0
37	3840743.0
38	5640748.0
39	1.3147468E7
40	9822.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.41931564447128	19.885461966498863	12.831541511077749	22.86368087795211
2	17.085175744140113	23.000042441760858	36.39448096288832	23.52030085121071
3	18.40224785558026	26.771630959487947	32.59469302566508	22.231428159266706
4	11.784208459738341	16.33584201052963	37.162698662125656	34.717250867606374
5	12.209183541147754	40.74380110066499	35.19998520909928	11.847030149087976
6	30.054466422481358	37.39797473829674	18.191763022855444	14.355795816366465
7	26.925068784334655	33.951738065289724	22.068200366802596	17.05499278357302
8	29.814133683958715	33.29778682497321	20.45932121439383	16.428758276674245
9	27.6856519525515	12.086358926815695	19.884712932989036	40.343276187643774
10	16.871068475445906	28.295164449181964	35.02939891043336	19.804368164938776
11	35.34004575212919	21.16568549539268	23.260863016213477	20.23340573626465
12	20.351355669527045	27.550741925677393	32.52558702945151	19.572315375344054
13	30.468614979198517	20.194499605760495	26.797861253516274	22.539024161524715
14	20.40826340215859	20.534110637069915	30.535910800675854	28.521715160095646
15	23.853518146734807	28.772860785481967	25.287041097154052	22.086579970629174
16	21.822813803146342	27.2165687406661	27.5366103411193	23.424007115068253
17	20.37639917989734	27.685087171086675	29.61862474391611	22.319888905099873
18	20.37681077106343	26.396780022175598	31.13723686578791	22.089172340973064
19	21.585033102569977	26.16622323219139	31.914368494473656	20.33437517076498
20	22.438735247250477	24.84524123668841	32.1044887091422	20.611534806918915
21	23.267309617853254	24.840485623298463	31.11821979840512	20.773984960443165
22	22.024101353684525	24.81608488935081	31.658813106097078	21.50100065086758
23	21.6314655746004	25.62288322029685	31.359048307737087	21.386602897365666
24	21.754802975575487	25.091529270594403	31.950585830935374	21.203081922894736
25	21.356210485519977	25.60839565074074	31.652780696465655	21.382613167273632
26	21.30964620752	24.961363927001724	31.55206236322054	22.176927502257733
27	21.287322429163297	25.179873329147952	31.351603629178328	22.18120061251042
28	21.874456313375116	25.261625933519582	31.57574449116851	21.28817326193679
29	20.894319660326797	25.858255506188765	31.62915733163348	21.61826750185096
30	21.168725128722198	25.40096921910071	31.855741760275475	21.574563891901615
31	21.512394008624444	25.763642683842868	31.40403979619072	21.31992351134197
32	21.390391510883966	25.410607479943682	31.929631992182944	21.269369016989412
33	21.372401125889535	25.602893024378442	31.453732458177715	21.57097339155431
34	21.97663290902839	25.286442585689343	31.486050655971486	21.250873849310782
35	21.603664019049138	25.59106183733148	31.615193029855476	21.190081113763906
36	21.483272011879222	26.442962323618207	30.6583826998042	21.415382964698377
37	21.718977613231807	25.744233927947786	31.233290365195188	21.303498093625223
38	22.15555026480988	26.00515228225355	30.224786685179993	21.614510767756574
39	21.811077125344028	26.161938319746657	30.23610777358018	21.79087678132914
40	21.67664725958836	25.92390137486375	30.33255772442198	22.06689364112591
41	22.283898812140947	26.129258768880142	30.022150538901638	21.564691880077273
42	21.39074331068934	26.37963566538125	30.44827091634866	21.78135010758075
43	21.591246116263267	26.133559040716424	29.710300924952097	22.564893918068208
44	21.816889790868625	26.304238650600947	29.989724668835322	21.88914688969511
45	21.74769440378397	26.601306496967048	29.96454851158487	21.68645058766411
46	21.96402842405579	26.69731279183117	28.873242090984057	22.465416693128983
47	22.266550835294282	26.866736822051724	29.172378762241184	21.69433358041281
48	23.10172499965219	27.105735626464504	27.822269933058084	21.97026944082522
49	22.280712675922484	27.255467439866514	27.900453617742393	22.56336626646861
50	22.27109631176697	27.17002166881261	28.18886003316588	22.37002198625454
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	8006.0
1	8169.5
2	8333.0
3	22399.0
4	36465.0
5	44886.0
6	53307.0
7	45225.0
8	37143.0
9	47646.0
10	58149.0
11	85911.0
12	113673.0
13	207999.0
14	302325.0
15	316191.0
16	330057.0
17	429220.5
18	528384.0
19	510450.0
20	492516.0
21	510932.0
22	529348.0
23	560202.0
24	591056.0
25	632061.0
26	673066.0
27	749619.0
28	826172.0
29	913617.0
30	1001062.0
31	1106650.0
32	1212238.0
33	1314578.5
34	1416919.0
35	1525563.0
36	1634207.0
37	1723625.0
38	1813043.0
39	1869739.5
40	1926436.0
41	1974249.5
42	2022063.0
43	2053582.5
44	2085102.0
45	2078487.5
46	2071873.0
47	2049181.5
48	2026490.0
49	1992910.5
50	1959331.0
51	1902256.0
52	1845181.0
53	1806960.5
54	1768740.0
55	1683332.0
56	1597924.0
57	1551399.5
58	1504875.0
59	1403450.5
60	1302026.0
61	1134517.0
62	967008.0
63	851084.0
64	735160.0
65	642361.5
66	549563.0
67	468682.5
68	387802.0
69	355120.5
70	322439.0
71	284740.0
72	247041.0
73	201855.0
74	156669.0
75	124400.0
76	92131.0
77	66974.5
78	41818.0
79	31745.5
80	21673.0
81	16417.5
82	11162.0
83	8451.5
84	5741.0
85	4268.5
86	2796.0
87	2052.0
88	1308.0
89	984.0
90	660.0
91	493.0
92	326.0
93	213.5
94	101.0
95	80.0
96	59.0
97	39.5
98	20.0
99	22.5
100	25.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.059084325461878716
2	7.07834069650646E-5
3	4.813271673624393E-5
4	0.0
5	0.0
6	0.0067697250421387785
7	8.125935119589416E-4
8	7.07834069650646E-5
9	1.3024146881571885E-4
10	4.530138045764134E-5
11	0.0
12	1.217474599799111E-4
13	0.001075907785868982
14	0.0
15	8.182561845161467E-4
16	4.700018222480289E-4
17	5.379538929344909E-5
18	3.114469906462842E-5
19	5.662672557205168E-6
20	4.869898399196444E-4
21	0.0038194726398348856
22	0.0
23	9.060276091528268E-5
24	0.0022707316954392724
25	0.001783741855519628
26	0.013771619659122967
27	0.011384803176260989
28	0.02915993233332801
29	0.01768735773243034
30	0.02179562667268269
31	0.01865001206715522
32	0.05602931361726653
33	0.032455607761621416
34	0.03157223084269741
35	0.035185015934194305
36	0.04062401292538987
37	0.03902147659170081
38	0.05798576698578091
39	0.049924952600599364
40	0.0538095459748421
41	0.05817829785272589
42	0.052524119304356534
43	0.05722413752683682
44	0.06462808189538258
45	0.06800869741203405
46	0.09339162714970622
47	0.06802568542970568
48	0.07580336618702697
49	0.07758710804254661
50	0.10466883954738032
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	3.5319012E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.325828256274036
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.39888750160436	25.936237250723117
2	12.028676811460917	8.739032960067107
3	5.336066225818528	5.815110758495648
4	2.9071613735031385	4.22420179068595
5	1.7855702112746392	3.243115841714075
6	1.204867724941239	2.6260690828645843
7	0.8520011582360731	2.1664753423761103
8	0.6433506360031968	1.8696195769613437
9	0.5035675464012633	1.646325738540504
>10	2.9180086598420267	19.617528390793105
>50	0.2530662047634374	6.367622522298963
>100	0.15628235491427317	10.546313410523513
>500	0.008689148789773919	2.1343152472796127
>1k	0.003399703432120867	2.2099406887368707
>5k	2.412867222113666E-4	0.6109474191157702
>10k+	1.6345229300972653E-4	2.2471439788236935
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	156117	0.44201972580659954	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	126008	0.3567710217941544	No Hit
TCTCTGAGCGGGCTGGCAAGGCAGACCGATATCGTATGCCGTCTTCTGCT	104533	0.29596807521116386	Illumina PCR Primer Index 1 (95% over 24bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	73171	0.20717170684162967	No Hit
TCTCTGAGCGGGCTGGCAAGGCAGACCGATCTCGTATGCCGTCTTCTGCT	58761	0.16637215106696643	Illumina Paired End PCR Primer 2 (96% over 29bp)
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	2.831336278602584E-6	0.0
2	0.0	0.0	0.0	2.831336278602584E-6	0.0
3	0.0	0.0	0.0	2.831336278602584E-6	0.0
4	0.0	0.0	0.0	1.4156681393012919E-5	0.0
5	0.0	2.831336278602584E-6	0.0	1.6988017671615504E-5	0.0
6	0.0	2.831336278602584E-6	0.0	1.6988017671615504E-5	0.0
7	0.0	2.831336278602584E-6	0.0	2.265069022882067E-5	0.0
8	0.0	2.831336278602584E-6	0.0	2.5482026507423255E-5	0.0
9	2.5482026507423255E-5	2.831336278602584E-6	0.0	6.512073440785942E-5	0.0
10	4.2470044179038755E-5	2.831336278602584E-6	0.0	7.644607952226976E-5	0.0
11	4.530138045764134E-5	5.662672557205168E-6	0.0	8.210875207947493E-5	0.0
12	4.530138045764134E-5	5.662672557205168E-6	0.0	1.047594423082956E-4	0.0
13	4.813271673624392E-5	5.662672557205168E-6	0.0	1.1325345114410335E-4	0.0
14	4.813271673624392E-5	5.662672557205168E-6	0.0	1.3307280509432143E-4	0.0
15	5.096405301484651E-5	8.494008835807752E-6	0.0	1.415668139301292E-4	0.0
16	5.096405301484651E-5	8.494008835807752E-6	0.0	1.5289215904453951E-4	0.0
17	5.379538929344909E-5	8.494008835807752E-6	0.0	1.7554284927336018E-4	0.0
18	5.379538929344909E-5	1.1325345114410336E-5	0.0	1.7554284927336018E-4	0.0
19	7.361474324366718E-5	1.1325345114410336E-5	0.0	1.925308669449757E-4	0.0
20	7.361474324366718E-5	1.1325345114410336E-5	0.0	2.1235022089519378E-4	0.0
21	7.361474324366718E-5	1.4156681393012919E-5	0.0	2.3500091112401444E-4	0.0
22	7.361474324366718E-5	1.4156681393012919E-5	0.0	2.689769464672455E-4	0.0
23	7.361474324366718E-5	1.6988017671615504E-5	0.0	3.0012164553187387E-4	0.0
24	7.361474324366718E-5	1.9819353950218088E-5	0.0	3.482543622681178E-4	0.0
25	7.361474324366718E-5	2.265069022882067E-5	0.0	3.8789307016855396E-4	0.0
26	7.361474324366718E-5	2.265069022882067E-5	0.0	4.21869105511785E-4	0.0
27	7.361474324366718E-5	2.5482026507423255E-5	0.0	4.8415850364104184E-4	0.0
28	7.361474324366718E-5	2.5482026507423255E-5	0.0	6.851833794218252E-4	0.0
29	7.361474324366718E-5	2.5482026507423255E-5	0.0	9.569916621676734E-4	0.0
30	7.361474324366718E-5	3.397603534323101E-5	0.0	0.0014043427941868815	0.0
31	7.361474324366718E-5	3.680737162183359E-5	0.0	0.001795067200634038	0.0
32	7.361474324366718E-5	3.680737162183359E-5	0.0	0.0024406118721554274	0.0
33	7.361474324366718E-5	3.680737162183359E-5	0.0	0.0036156164277754994	0.0
34	7.361474324366718E-5	3.680737162183359E-5	0.0	0.005617371176747526	0.0
35	7.644607952226976E-5	4.2470044179038755E-5	0.0	0.008550635561379803	2.831336278602584E-6
36	7.644607952226976E-5	4.2470044179038755E-5	0.0	0.013853728411202443	2.831336278602584E-6
37	7.644607952226976E-5	4.813271673624392E-5	0.0	0.023381174988700137	2.831336278602584E-6
38	7.644607952226976E-5	5.096405301484651E-5	0.0	0.03711881861247987	2.831336278602584E-6
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	81190	0.0	35.55013	1
TATGCCG	27255	0.0	32.734127	35
CCGTCTT	26750	0.0	31.344633	39
ATGCCGT	27475	0.0	31.150345	36
GCCGTCT	26980	0.0	31.128527	38
TGCCGTC	28370	0.0	30.144829	37
TCGTATG	29725	0.0	30.109863	32
GACCGAT	30435	0.0	30.097488	24
CGTCTTC	27945	0.0	29.798594	40
CGTATGC	30290	0.0	29.509722	33
TGAGCGG	34975	0.0	29.503725	5
TATCGTA	18110	0.0	29.295656	30
GCGGGCT	35040	0.0	29.271475	8
ACCGATA	18910	0.0	29.246588	25
AGACCGA	33570	0.0	29.167286	23
ATATCGT	18255	0.0	29.026794	29
CGATATC	18895	0.0	28.964024	27
CCGATAT	18960	0.0	28.854265	26
CAGACCG	34560	0.0	28.713678	22
AGCGGGC	35965	0.0	28.549322	7
>>END_MODULE