##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR522108_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 26676300 Sequences flagged as poor quality 0 Sequence length 50 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.7580090192418 40.0 39.0 40.0 37.0 40.0 2 38.64828450722177 40.0 39.0 40.0 36.0 40.0 3 38.529391519813466 40.0 39.0 40.0 36.0 40.0 4 38.627219441976585 40.0 39.0 40.0 36.0 40.0 5 38.670358857862595 40.0 39.0 40.0 36.0 40.0 6 38.714289050580476 40.0 39.0 40.0 36.0 40.0 7 38.664694204218726 40.0 39.0 40.0 36.0 40.0 8 38.61403586704303 40.0 39.0 40.0 36.0 40.0 9 38.58916135296124 40.0 39.0 40.0 36.0 40.0 10 38.54516278494394 40.0 39.0 40.0 36.0 40.0 11 38.577691921293436 40.0 39.0 40.0 36.0 40.0 12 38.462737598542525 40.0 38.0 40.0 35.0 40.0 13 38.46088018203424 40.0 38.0 40.0 35.0 40.0 14 38.36530590824065 40.0 38.0 40.0 35.0 40.0 15 38.36944025220889 40.0 38.0 40.0 35.0 40.0 16 38.215601264043364 40.0 38.0 40.0 35.0 40.0 17 38.22242612356286 40.0 38.0 40.0 35.0 40.0 18 38.166141668822135 40.0 38.0 40.0 35.0 40.0 19 38.106923936228036 40.0 38.0 40.0 35.0 40.0 20 38.03162961130292 40.0 38.0 40.0 35.0 40.0 21 38.28804170743319 40.0 38.0 40.0 35.0 40.0 22 38.27319673268032 40.0 38.0 40.0 35.0 40.0 23 38.246891285523105 40.0 38.0 40.0 35.0 40.0 24 38.166783212064644 40.0 38.0 40.0 35.0 40.0 25 38.12804665564565 40.0 38.0 40.0 35.0 40.0 26 38.083493063130945 40.0 38.0 40.0 35.0 40.0 27 37.971866113366545 40.0 38.0 40.0 35.0 40.0 28 37.894760180384836 40.0 38.0 40.0 35.0 40.0 29 37.81479785427514 40.0 38.0 40.0 34.0 40.0 30 37.6969639717652 40.0 38.0 40.0 34.0 40.0 31 37.30331743907514 40.0 38.0 40.0 33.0 40.0 32 37.21192755367124 39.0 38.0 40.0 33.0 40.0 33 37.027486720422246 39.0 38.0 40.0 33.0 40.0 34 36.89480745080839 39.0 38.0 40.0 32.0 40.0 35 36.68813287449909 39.0 37.0 40.0 32.0 40.0 36 36.35731293320288 39.0 37.0 40.0 31.0 40.0 37 36.21694717783201 39.0 37.0 40.0 31.0 40.0 38 36.0328772730851 39.0 36.0 40.0 30.0 40.0 39 35.80955567301312 39.0 36.0 40.0 29.0 40.0 40 35.56500223044425 39.0 36.0 40.0 29.0 40.0 41 35.042846946540564 39.0 36.0 40.0 27.0 40.0 42 34.79668934597377 39.0 35.0 40.0 25.0 40.0 43 34.537239272312874 39.0 35.0 40.0 23.0 40.0 44 34.16573003002665 39.0 35.0 40.0 19.0 40.0 45 33.60686309570668 39.0 35.0 40.0 2.0 40.0 46 32.68585714660579 38.0 33.0 40.0 2.0 40.0 47 32.42379430430757 38.0 33.0 40.0 2.0 40.0 48 32.11511930065264 38.0 33.0 40.0 2.0 40.0 49 31.88012115623231 38.0 33.0 40.0 2.0 40.0 50 31.663258622822504 38.0 32.0 40.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1 1 -0.2805511532554448 1 2 -0.4232189502079322 1 3 -0.3519812784035352 1 4 -0.3329859610260897 1 5 -0.32096058295815766 1 6 -0.38184398544967024 1 7 -0.3618553052037541 1 8 -0.3089850438741166 1 9 -0.2910742796856596 1 10 -0.32282972863620785 1 11 -0.3437953652758239 1 12 -0.3488697215723704 1 13 -0.30389664900355484 1 14 -0.34968634116065545 1 15 -0.34964180945667067 1 16 -0.3382072681107502 1 17 -0.36220697007628644 1 18 -0.3511857297652128 1 19 -0.25847689227177995 1 20 -0.3109564898481736 1 21 -0.18598586997454447 1 22 -0.17076027910562885 1 23 -0.20242775303029248 1 24 -0.11212377373504978 1 25 -0.09959651958948967 1 26 -0.28900670776023674 1 27 -0.11721670409234264 1 28 -0.14826995981838564 1 29 -0.06673740359202895 1 30 -0.17678623275106276 1 31 -0.154398803526 1 32 0.04187322344980515 1 33 0.02762299885545616 1 34 0.0833246567027004 1 35 0.01368389334849951 1 36 0.021062548224051625 1 37 -0.09254006573149809 1 38 0.14139359032660082 1 39 0.13659725493439367 1 40 0.13119675482341364 1 41 0.2486258386199438 1 42 0.21658355753638148 1 43 0.3048791733626146 1 44 0.2996955558435914 1 45 0.19768002793918527 1 46 0.1787850432650373 1 47 0.30593044182217 1 48 0.2213077243417203 1 49 0.27154027791640445 1 50 0.3536952892758656 2 1 -0.15798061254483287 2 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-1.8284519789462337 120 43 -2.076433286520519 120 44 -2.397421109337376 120 45 -1.9321592673086805 120 46 -1.95072894315458 120 47 -1.9279344801252485 120 48 -2.0250964587708182 120 49 -1.7961444001836995 120 50 -2.0673571008012175 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 12641.0 3 1170.0 4 1405.0 5 1367.0 6 2026.0 7 3419.0 8 3722.0 9 4610.0 10 6984.0 11 9937.0 12 11678.0 13 15962.0 14 20565.0 15 24136.0 16 27720.0 17 33559.0 18 41117.0 19 50293.0 20 63621.0 21 67172.0 22 90269.0 23 134751.0 24 178105.0 25 153845.0 26 239772.0 27 286830.0 28 299615.0 29 303605.0 30 390539.0 31 477948.0 32 591349.0 33 670327.0 34 939626.0 35 1282681.0 36 1601756.0 37 2391804.0 38 3955998.0 39 1.2240533E7 40 43843.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.6211309444439 18.744174237462047 13.449574209923357 20.185120608170696 2 16.90762587015441 23.682418476325427 37.45257400763974 21.957381645880425 3 18.67294740717027 28.498920013525137 33.196572533031244 19.631560046273353 4 10.747510609027113 16.33552322091424 36.67079857953765 36.246167590521 5 10.636996135146179 43.1577167748151 34.362216649235464 11.84307044080326 6 27.619598279350065 36.69437313764228 19.93518266976491 15.750845913242737 7 26.09225304290341 34.76800084040157 22.242868180786665 16.896877935908357 8 30.960170638356892 32.0626923523877 21.044095320565447 15.933041688689961 9 28.60826651372192 11.341366681286386 21.06090799698609 38.98945880800561 10 18.133098167184762 28.557145092952794 34.640888358244766 18.668868381617678 11 33.979127540176115 21.222534609372364 23.839291805835142 20.959046044616382 12 19.96381807072195 29.76876103507608 32.66478109782841 17.60263979637356 13 31.328377736747292 20.3498996967398 27.746006519243327 20.575716047269573 14 19.817227711207128 21.4894677491075 33.0344133025574 25.658891237127975 15 23.70110690898371 28.66813970629526 27.34399723855677 20.286756146164258 16 19.730842642254878 27.05606637744355 30.189987806025957 23.023103174275615 17 18.395832135328522 27.993093344511294 32.40665177754424 21.204422742615936 18 18.04132876203283 25.316332417901155 35.19034450406501 21.451994316001 19 20.11718124737173 25.154150042348476 36.539949607670216 18.18871910260958 20 20.47354183865955 23.714436013848875 37.184594430798725 18.627427716692853 21 21.839436851191486 23.735848216314064 35.68736795904667 18.73734697344778 22 20.363558664432475 23.837342510018257 36.40571218647264 19.393386639076635 23 19.577024309698746 24.756903574853382 36.19574988783049 19.470322227617388 24 19.60522997667951 24.273702757676347 36.9931409066452 19.12792635899894 25 19.292513012188696 24.715203618138588 36.29058771584609 19.701695653826633 26 19.044992920274886 24.152131310680414 36.43841613907393 20.364459629970767 27 19.400937813171453 24.28858131669509 36.067794436931564 20.2426864332019 28 19.889217452062894 24.378486327818504 36.40461844306202 19.327677777056582 29 18.68406383201445 25.051694645562495 36.394957589994725 19.869283932428335 30 18.987214626321713 24.65650851564632 36.36454268486323 19.991734173168734 31 19.657556424168956 25.24249596158758 35.87409001681653 19.225857597426934 32 19.473174205559094 24.867631780614193 36.32125057536928 19.337943438457437 33 19.297312782730774 25.095727240547642 36.10404492494511 19.50291505177648 34 19.914107326094616 24.67858962174563 35.95236117670638 19.454941875453375 35 19.922397866916242 25.110923130167677 35.57051778875916 19.396161214156916 36 19.59603459742681 26.080530200372042 34.44231252773882 19.881122674462322 37 19.993207855921643 25.548766953218994 34.96257664365706 19.495448547202308 38 20.201590611045123 25.863974361383267 33.86331699172674 20.071118035844872 39 20.205680222473294 26.01804328555143 33.77217537017931 20.004101121795966 40 20.032133225372498 25.821064289328042 33.788356506284316 20.358445979015144 41 20.746267878575065 26.136737987115488 33.31829031213056 19.798703822178886 42 19.476178636698926 26.54528149816401 33.58981022595263 20.38872963918443 43 19.867666066955977 26.41439584708694 32.592826223916724 21.12511186204036 44 20.140596292327732 26.771025257003377 32.557661167496555 20.530717283172333 45 20.14938611362595 27.034283895509997 32.59295228695908 20.223377703904973 46 20.504872915403368 27.397246961606175 30.91825716819906 21.179622954791398 47 21.326586541606673 27.729701858194787 30.951050212251086 19.992661387947454 48 22.084908315251816 28.037148954057955 29.1858557314437 20.692086999246527 49 21.167265065027287 28.58482507773677 29.102605030447602 21.14530482678834 50 20.957797601121428 28.202459065542484 29.439027408561397 21.40071592477469 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 15262.0 1 13357.5 2 11453.0 3 29598.5 4 47744.0 5 56801.5 6 65859.0 7 56252.5 8 46646.0 9 60703.0 10 74760.0 11 108957.5 12 143155.0 13 256821.5 14 370488.0 15 394645.5 16 418803.0 17 547620.0 18 676437.0 19 646494.0 20 616551.0 21 631134.5 22 645718.0 23 660263.5 24 674809.0 25 698756.0 26 722703.0 27 764571.5 28 806440.0 29 861865.5 30 917291.0 31 984437.0 32 1051583.0 33 1112159.5 34 1172736.0 35 1236400.0 36 1300064.0 37 1344775.0 38 1389486.0 39 1402421.5 40 1415357.0 41 1428444.5 42 1441532.0 43 1439753.0 44 1437974.0 45 1421855.5 46 1405737.0 47 1370518.5 48 1335300.0 49 1285225.5 50 1235151.0 51 1191257.0 52 1147363.0 53 1114684.5 54 1082006.0 55 1023438.0 56 964870.0 57 945466.0 58 926062.0 59 837058.5 60 748055.0 61 665123.5 62 582192.0 63 517304.0 64 452416.0 65 405321.5 66 358227.0 67 288865.0 68 219503.0 69 217273.5 70 215044.0 71 234185.5 72 253327.0 73 198827.5 74 144328.0 75 113103.5 76 81879.0 77 57087.5 78 32296.0 79 23696.5 80 15097.0 81 11255.0 82 7413.0 83 5612.0 84 3811.0 85 2829.0 86 1847.0 87 1340.0 88 833.0 89 606.5 90 380.0 91 270.5 92 161.0 93 113.5 94 66.0 95 44.5 96 23.0 97 23.0 98 23.0 99 31.0 100 39.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05858008794323051 2 0.0 3 1.499458320681654E-4 4 7.384832229357144E-4 5 0.0 6 3.7861322597211757E-4 7 0.012509231040286697 8 0.0 9 0.0 10 4.685807252130168E-4 11 0.0 12 0.0 13 7.422318687374186E-4 14 1.724377068783902E-4 15 0.0014769664458714289 16 3.373781221533721E-5 17 7.497291603408269E-6 18 4.123510381874548E-5 19 6.747562443067442E-5 20 1.9118093588691088E-4 21 0.0032388299726723723 22 0.0 23 5.885373908675491E-4 24 0.0023129144596514507 25 0.0034712460123780286 26 0.011051007823423787 27 0.013532611344151924 28 0.028710878195251966 29 0.016089187780914147 30 0.018900672132192247 31 0.018473326510797975 32 0.048691160318335 33 0.02931441016932633 34 0.030495233596863132 35 0.025978115405809653 36 0.038067498116305484 37 0.03823243853158047 38 0.046704378043431805 39 0.057361778057676664 40 0.0461458298189779 41 0.05618845192174327 42 0.05292713007426067 43 0.05664578670955117 44 0.05942728189441564 45 0.06696955724744436 46 0.09297016452806424 47 0.06488156153589515 48 0.06868268837882315 49 0.07133672960642969 50 0.09751727188553136 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 2.66763E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 36.79163681587889 #Duplication Level Percentage of deduplicated Percentage of total 1 69.61213119763931 25.61144249002858 2 12.465838851331908 9.172772312469528 3 5.747670505578921 6.343986173357961 4 3.2471973265659044 4.778788188340226 5 2.0462664473228616 3.7642745979210748 6 1.356086091188955 2.9935576174853313 7 1.0218679442543115 2.6317335995155378 8 0.7209845292414451 2.1220960759774927 9 0.5561454107051407 1.8415349970733204 >10 2.9187207630511955 18.66511186799242 >50 0.18566355892750985 4.71696452903081 >100 0.10967144086134041 7.396722936103284 >500 0.007022140464784073 1.7517796758781137 >1k 0.004008050455188917 2.9421987747741407 >5k 3.782037917386555E-4 1.0155049947534578 >10k+ 3.4753861943337633E-4 4.251531169298603 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 202666 0.7597230500481701 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 148626 0.5571462309240787 No Hit TCTCTGAGCGGGCTGGCAAGGCAGACCGATATCGTATGCCGTCTTCTGCT 96655 0.3623253599637131 Illumina PCR Primer Index 1 (95% over 24bp) GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 96649 0.3623028680889029 No Hit GTACATGGGGGAGCCATTGTGGCTCCGGCCGGTTGCGCGGGCCCTCGGAC 90214 0.3381803323549368 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 37017 0.13876362164168193 No Hit TCTCTGAGCGGGCTGGCAAGGCAGACCGATCTCGTATGCCGTCTTCTGCT 36486 0.13677309072097707 Illumina Paired End PCR Primer 2 (96% over 29bp) GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 28569 0.10709506190888542 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 28481 0.10676518107833545 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 26842 0.10062115060934237 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 3.7486458017041343E-6 0.0 2 0.0 0.0 0.0 7.4972916034082685E-6 0.0 3 0.0 3.7486458017041343E-6 0.0 7.4972916034082685E-6 0.0 4 0.0 7.4972916034082685E-6 0.0 1.8743229008520673E-5 0.0 5 0.0 1.1245937405112402E-5 0.0 2.2491874810224805E-5 0.0 6 3.7486458017041343E-6 1.1245937405112402E-5 0.0 2.624052061192894E-5 0.0 7 3.7486458017041343E-6 1.4994583206816537E-5 0.0 2.624052061192894E-5 0.0 8 7.4972916034082685E-6 1.4994583206816537E-5 0.0 2.9989166413633074E-5 0.0 9 7.4972916034082685E-6 1.4994583206816537E-5 0.0 5.622968702556202E-5 0.0 10 1.8743229008520673E-5 1.4994583206816537E-5 0.0 7.872156183578682E-5 0.0 11 1.8743229008520673E-5 1.4994583206816537E-5 0.0 1.424485404647571E-4 0.0 12 1.8743229008520673E-5 1.4994583206816537E-5 0.0 1.724377068783902E-4 3.7486458017041343E-6 13 1.8743229008520673E-5 1.4994583206816537E-5 0.0 1.9118093588691085E-4 3.7486458017041343E-6 14 8.62188534391951E-5 1.4994583206816537E-5 0.0 2.2117010230054393E-4 3.7486458017041343E-6 15 8.62188534391951E-5 1.4994583206816537E-5 0.0 2.6990249772269767E-4 3.7486458017041343E-6 16 8.996749924089922E-5 1.4994583206816537E-5 0.0 3.148862473431473E-4 3.7486458017041343E-6 17 9.74647908443075E-5 1.4994583206816537E-5 0.0 3.448754137567804E-4 3.7486458017041343E-6 18 9.74647908443075E-5 2.624052061192894E-5 0.0 4.0485374658404653E-4 3.7486458017041343E-6 19 1.1245937405112403E-4 2.9989166413633074E-5 0.0 4.6108343360960855E-4 3.7486458017041343E-6 20 1.1245937405112403E-4 2.9989166413633074E-5 0.0 5.135644748334664E-4 3.7486458017041343E-6 21 1.199566656545323E-4 2.9989166413633074E-5 0.0 5.84788745065845E-4 3.7486458017041343E-6 22 1.199566656545323E-4 3.373781221533721E-5 0.0 6.635103069016318E-4 3.7486458017041343E-6 23 1.2370531145623645E-4 3.748645801704135E-5 0.0 7.384832229357144E-4 3.7486458017041343E-6 24 1.2745395725794056E-4 4.123510381874548E-5 0.0 8.396966595817261E-4 7.4972916034082685E-6 25 1.2745395725794056E-4 5.622968702556202E-5 0.0 9.146695756158088E-4 7.4972916034082685E-6 26 1.2745395725794056E-4 5.622968702556202E-5 0.0 9.409100962277377E-4 7.4972916034082685E-6 27 1.312026030596447E-4 5.997833282726615E-5 0.0 0.0010533694702788618 7.4972916034082685E-6 28 1.499458320681654E-4 6.372697862897028E-5 0.0 0.001229555822958956 7.4972916034082685E-6 29 1.499458320681654E-4 6.372697862897028E-5 0.0 0.001454474571061204 7.4972916034082685E-6 30 1.499458320681654E-4 6.372697862897028E-5 0.0 0.001814344568024801 7.4972916034082685E-6 31 1.499458320681654E-4 6.372697862897028E-5 0.0 0.002204203731402031 7.4972916034082685E-6 32 1.499458320681654E-4 7.122427023237856E-5 0.0 0.002788992476467876 7.4972916034082685E-6 33 1.499458320681654E-4 7.122427023237856E-5 0.0 0.0037786349681177673 7.4972916034082685E-6 34 1.536944778698695E-4 7.122427023237856E-5 0.0 0.005345568913230096 7.4972916034082685E-6 35 1.6119176947327777E-4 8.996749924089922E-5 0.0 0.007943380453811062 7.4972916034082685E-6 36 1.6119176947327777E-4 9.371614504260336E-5 0.0 0.012460498644864542 7.4972916034082685E-6 37 1.6119176947327777E-4 9.371614504260336E-5 0.0 0.019519198689473428 7.4972916034082685E-6 38 1.6119176947327777E-4 9.371614504260336E-5 0.0 0.030180347349519986 7.4972916034082685E-6 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 106495 0.0 36.39109 1 TATGCCG 20610 0.0 34.17759 35 CCGTCTT 20530 0.0 33.284264 39 GCCGTCT 20715 0.0 32.986023 38 CGTCTTC 20675 0.0 32.899372 40 ATGCCGT 21065 0.0 32.68715 36 GACCGAT 21695 0.0 32.39822 24 TCGTATG 21645 0.0 32.30767 32 GATATCG 15470 0.0 32.058228 28 CGTATGC 21980 0.0 32.006252 33 TATCGTA 15325 0.0 31.959846 30 ACCGATA 15635 0.0 31.883219 25 CGATATC 15595 0.0 31.801685 27 AGACCGA 22980 0.0 31.716097 23 ATCGTAT 15710 0.0 31.709068 31 TGAGCGG 24025 0.0 31.566282 5 ATATCGT 15470 0.0 31.560015 29 CCGATAT 15705 0.0 31.5511 26 TGCCGTC 21845 0.0 31.379578 37 GCGGGCT 24045 0.0 31.3712 8 >>END_MODULE