FastQCFastQC Report
Thu 26 May 2016
SRR522107_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR522107_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35312070
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAG3319040.9399165780992165Illumina Paired End PCR Primer 2 (97% over 36bp)
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT555810.15739943877546686No Hit
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGT363060.10281470330116586Illumina Paired End PCR Primer 2 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGA511250.037.8210525
TGCCGAG527250.036.6165726
AGATCGG463550.036.22157731
AATGCCG538050.036.06605524
CGAGATC488000.035.40296629
CCGAGAT498300.034.7786228
GAGATCG497300.034.45803530
GCCGAGA578650.033.59390327
TATGCCG56450.032.6344343
TCGTATG57750.031.82190540
GTTCAGC636500.030.9485514
GACCGAT60200.030.34050832
CAGGAAT661850.029.91680320
CTCGTAT61950.029.80482139
GGAATGC662850.029.7220622
GAATGCC657800.029.70666123
AGGAATG676700.029.20302221
CGTATGC63850.029.02357541
ACCGATC63900.028.85929133
GCAGGAA710300.028.08732619
Produced by FastQC (version 0.11.5)