##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR522107_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	35312070
Sequences flagged as poor quality	0
Sequence length	50
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.99097518214027	39.0	38.0	40.0	34.0	40.0
2	37.605652996270116	39.0	38.0	40.0	33.0	40.0
3	37.71450172702988	39.0	38.0	40.0	33.0	40.0
4	37.680893728405046	39.0	38.0	40.0	33.0	40.0
5	37.66514424104846	39.0	38.0	40.0	33.0	40.0
6	37.67180289912203	39.0	38.0	40.0	33.0	40.0
7	37.66030790605026	39.0	38.0	40.0	33.0	40.0
8	37.604880257656944	39.0	38.0	40.0	33.0	40.0
9	37.46192721072426	39.0	37.0	40.0	33.0	40.0
10	37.33191288417813	39.0	37.0	40.0	33.0	40.0
11	37.46743359989941	39.0	37.0	40.0	33.0	40.0
12	37.473336340803584	39.0	37.0	40.0	33.0	40.0
13	37.43739828902695	39.0	37.0	40.0	33.0	40.0
14	37.40566044414842	39.0	37.0	40.0	33.0	40.0
15	37.40946438427427	39.0	37.0	40.0	33.0	40.0
16	37.507812088048084	39.0	37.0	40.0	33.0	40.0
17	37.35460770212565	39.0	37.0	40.0	33.0	40.0
18	37.24763042778291	39.0	36.0	40.0	33.0	40.0
19	37.23636204278027	39.0	36.0	40.0	33.0	40.0
20	37.20710411482533	39.0	36.0	40.0	33.0	40.0
21	37.257617664441646	39.0	36.0	40.0	33.0	40.0
22	37.19939094479593	39.0	36.0	40.0	33.0	40.0
23	37.09668269234854	39.0	36.0	40.0	32.0	40.0
24	37.07768479729452	39.0	36.0	40.0	32.0	40.0
25	37.04196199769654	39.0	36.0	40.0	32.0	40.0
26	36.885363361592795	39.0	36.0	40.0	32.0	40.0
27	36.82376391415173	39.0	36.0	40.0	31.0	40.0
28	36.77902159233373	39.0	36.0	40.0	31.0	40.0
29	36.70299104527149	39.0	36.0	40.0	31.0	40.0
30	36.580681449713936	38.0	35.0	40.0	31.0	40.0
31	36.55650371105404	38.0	36.0	40.0	31.0	40.0
32	36.60230261210968	39.0	36.0	40.0	31.0	40.0
33	36.51379890785219	38.0	35.0	40.0	31.0	40.0
34	36.40079281673377	38.0	35.0	40.0	31.0	40.0
35	36.40146213461856	38.0	35.0	40.0	31.0	40.0
36	36.50177420921515	39.0	36.0	40.0	31.0	40.0
37	36.2434789860804	39.0	35.0	40.0	30.0	40.0
38	36.0946836874757	38.0	35.0	40.0	30.0	40.0
39	36.08194050929328	38.0	35.0	40.0	30.0	40.0
40	35.65117338065993	38.0	35.0	40.0	29.0	40.0
41	35.87727068959707	38.0	35.0	40.0	29.0	40.0
42	35.86351618582541	38.0	35.0	40.0	30.0	40.0
43	35.83670640095582	38.0	35.0	40.0	30.0	40.0
44	35.66679582930143	38.0	35.0	40.0	29.0	40.0
45	35.654384633922625	38.0	35.0	40.0	29.0	40.0
46	35.728055591190206	38.0	35.0	40.0	30.0	40.0
47	35.50151596890242	38.0	35.0	40.0	29.0	40.0
48	35.256118800172295	38.0	35.0	39.0	29.0	40.0
49	35.114243854863226	38.0	34.0	39.0	29.0	40.0
50	35.110793703116244	38.0	35.0	39.0	29.0	40.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1	1	-0.2537150340398142
1	2	-0.1272575638726039
1	3	-0.2474403267260783
1	4	-0.14639511272583405
1	5	-0.1741665046688894
1	6	-0.09280182186117258
1	7	-0.14490583427023296
1	8	-0.17480652606032265
1	9	-1.6566118393512852E-4
1	10	0.09769106839336672
1	11	0.031060248130295065
1	12	-0.29810778720821673
1	13	-0.0434836474968705
1	14	-0.46954035070714895
1	15	-0.1656353379416089
1	16	-0.2302753315308408
1	17	-0.045646508186244716
1	18	-0.13119015340988227
1	19	-0.025922073700698434
1	20	-0.08885659684431602
1	21	-0.12750681606921432
1	22	-0.04760200657758418
1	23	-0.10696856646547559
1	24	-0.09874219757611513
1	25	-0.1843181572953796
1	26	-0.1950134274685027
1	27	-0.22224409342586426
1	28	-0.5796786536417216
1	29	-0.2207445185210446
1	30	-0.5612088154968475
1	31	-0.6550177129974131
1	32	-0.6728905388375424
1	33	-0.7170186828097371
1	34	-0.5115210230961367
1	35	-0.16466213687171205
1	36	-0.08611237747876999
1	37	0.28094713866104826
1	38	0.3175326281375561
1	39	0.2691204358445347
1	40	0.6032158768067362
1	41	0.4488165369777022
1	42	0.36206021693057977
1	43	0.06744588444934152
1	44	0.19280052908277412
1	45	0.18655661392270417
1	46	0.23301950387265435
1	47	0.3030312679910523
1	48	0.37929389189513785
1	49	0.4595894145251833
1	50	0.1644542571785479
2	1	-0.1468056589995399
2	2	-0.10631427281482075
2	3	-0.24837037055097966
2	4	-0.17451459801817037
2	5	-0.1427649115522911
2	6	-0.1138667468870338
2	7	-0.19360343607493746
2	8	-0.7480638938698547
2	9	0.0018344796594007562
2	10	0.048593297263892055
2	11	-0.8728932989514036
2	12	-0.2101197654929905
2	13	-0.167943994952914
2	14	-0.15066975761101276
2	15	-0.29688879101910715
2	16	-0.22690039710669652
2	17	-0.07609972878992721
2	18	-0.1589101689849599
2	19	-0.01509858783695961
2	20	-0.12804636895449306
2	21	-0.07934443150259796
2	22	-0.08835480475660518
2	23	-0.1935216345267392
2	24	-0.1755360577037024
2	25	-0.23700768150580842
2	26	-0.26395233209417057
2	27	-0.2525507966683378
2	28	-0.6926260427698594
2	29	-0.24131775115566967
2	30	-0.49249706417133154
2	31	-0.8185167389462222
2	32	-0.22593497805495844
2	33	-0.14761847139328665
2	34	-0.6549023071992295
2	35	-0.32483529248860776
2	36	-0.17687672782397357
2	37	0.11843893082454571
2	38	0.2207483248955242
2	39	-0.4446904457091634
2	40	0.47194499321380334
2	41	0.332428013064451
2	42	0.19166142916603235
2	43	-0.020504137260715538
2	44	0.0560201601864776
2	45	-0.010938066467524266
2	46	-0.24905263897100127
2	47	0.020599404042556557
2	48	0.06376570764676615
2	49	0.21028949530268903
2	50	0.11236507234854543
3	1	-0.13666304005208474
3	2	-0.059477614537918555
3	3	-0.18446292324718172
3	4	-0.16612516795623122
3	5	-0.18321828917777339
3	6	-0.08686360258145243
3	7	-0.1314618908177323
3	8	-0.13849070748153025
3	9	-0.04163261081041014
3	10	0.16252187524317918
3	11	-0.1070767544632929
3	12	-0.1760021551955404
3	13	-0.14150475304332844
3	14	-0.2233320082943564
3	15	-0.10077892210153294
3	16	-0.1210996683648915
3	17	-0.21016420837592875
3	18	-0.022832858249920207
3	19	-0.007382233770762525
3	20	-0.07877228100661426
3	21	-0.07364961456143959
3	22	-0.2113488492796023
3	23	-0.13505198793839668
3	24	-0.13780970267595904
3	25	-0.19980290982044124
3	26	-0.17197271393391844
3	27	-0.2962812847183258
3	28	-0.39490070010859313
3	29	-0.3933990261507887
3	30	-0.2866954961090258
3	31	-0.4614787905248008
3	32	-0.5454556459681896
3	33	-1.0890666620273706
3	34	-0.3261130584201908
3	35	-0.33399753843548297
3	36	-0.2438980861792146
3	37	-0.0303211495491027
3	38	-0.2015001543558057
3	39	0.08080267575483191
3	40	0.23952519461003874
3	41	0.27500907166486854
3	42	-0.07858817528470041
3	43	0.018547696936430214
3	44	0.07858178061548671
3	45	0.06623872722099833
3	46	-0.10834172063483294
3	47	-0.057099211437531494
3	48	0.1999236090463299
3	49	0.21966575094476326
3	50	0.04293085181449641
4	1	-0.13207335160422673
4	2	-0.004203352444307029
4	3	-0.16906987117452132
4	4	-0.17344509274322917
4	5	-0.15926965315417618
4	6	-0.04258472272952929
4	7	-0.0959974910651411
4	8	-0.13099133107354533
4	9	0.024582085842283163
4	10	0.03941361363114737
4	11	0.09156963318012146
4	12	-0.3202409609994845
4	13	-0.07445010820126896
4	14	-0.004405336643621638
4	15	-0.09584766455815696
4	16	-0.2911304524763949
4	17	-0.12368258385795627
4	18	-0.07953597039155369
4	19	-0.04918337801682071
4	20	-0.0837366275095448
4	21	-0.042114158908923116
4	22	-0.021833164456886323
4	23	-0.18522747904527392
4	24	-0.010663867563920348
4	25	-0.04939469285783815
4	26	-0.05020758944035464
4	27	-0.22232557782050577
4	28	-0.21397537059760907
4	29	-0.2553521263344578
4	30	-0.07152179625975208
4	31	-0.26089993497524944
4	32	-0.1290986535675671
4	33	-0.23552291222835464
4	34	-0.1327917174427924
4	35	-0.8798816237551179
4	36	-0.8659516539929726
4	37	0.18458850492125123
4	38	0.022540915040721643
4	39	0.14173597135240357
4	40	0.45274201715992035
4	41	0.3842656803555755
4	42	0.25606015138445315
4	43	0.19230126957168636
4	44	0.29645811751127127
4	45	0.16302007564276977
4	46	0.12831203185327666
4	47	0.05107912901573286
4	48	0.20214587160731412
4	49	0.3229141352169975
4	50	0.1837631698978015
5	1	-0.1800633271687957
5	2	-0.10094817538439571
5	3	-0.1969909440802482
5	4	-0.2266445790553604
5	5	-0.2149451502133033
5	6	-0.27580967261474854
5	7	-0.32952980803349874
5	8	-0.13624720736416407
5	9	-0.13883308578350295
5	10	-0.8616482833437047
5	11	-0.1082328015436147
5	12	-0.1346801254745884
5	13	-0.039324545806898925
5	14	-0.05050099660696361
5	15	-0.41904983264389273
5	16	-0.08902512575946986
5	17	-0.16179500983021455
5	18	0.11014119770930364
5	19	0.0883230928249148
5	20	-0.04963123310128026
5	21	-0.029663330079891637
5	22	-0.026619217809091822
5	23	-0.002130781394527048
5	24	-0.34511360385338463
5	25	-0.03930179304754233
5	26	-0.08149600402379775
5	27	-0.04560871335328898
5	28	-0.08896240538685873
5	29	-0.018657255839016784
5	30	-0.170656763784379
5	31	-0.3597165448168198
5	32	0.0031702334422760714
5	33	-0.10562115508350445
5	34	0.05248553875763662
5	35	-0.05994659635020838
5	36	0.21961277952495806
5	37	0.3246191165988819
5	38	0.42864784188143545
5	39	0.1263949928636663
5	40	0.2509093799987454
5	41	0.344789554526578
5	42	0.4506173811187111
5	43	0.29572830163446184
5	44	0.318478431433725
5	45	0.27665455174977893
5	46	0.29422541389345724
5	47	0.43899652897724195
5	48	0.5521007395658444
5	49	0.585119213223706
5	50	0.2843059509255923
6	1	-0.25858286043043677
6	2	-0.07344356797393203
6	3	-0.18980804601997647
6	4	-0.20643397648857587
6	5	-0.16816726755034495
6	6	-0.11730800265243602
6	7	-0.1357049500746541
6	8	-0.5238876386990157
6	9	0.004903302301727308
6	10	-0.28611307284287335
6	11	-0.2223463348255379
6	12	-0.11459182624343356
6	13	-0.09990908673329102
6	14	-0.13213187052657105
6	15	-0.18030122300379503
6	16	-0.07327837441332719
6	17	-0.02352683611590578
6	18	-0.9236336745041882
6	19	-0.06698229572506165
6	20	-0.011269739764209419
6	21	-0.14509518485105843
6	22	-0.1928296921942234
6	23	-0.05019980348503594
6	24	-0.07968230407410459
6	25	-0.09636858982250374
6	26	-0.01575999796684613
6	27	-0.15205939643374222
6	28	-0.18848354707493797
6	29	-0.08400705789672713
6	30	-0.0391701148487158
6	31	-0.15958532207492482
6	32	0.030380653701350013
6	33	-0.016088504933925662
6	34	0.005852408862942582
6	35	-0.35523460955158725
6	36	-0.028787294272177633
6	37	0.11836428422645895
6	38	0.12614067653438354
6	39	0.14333324973306105
6	40	0.3229434100184392
6	41	0.2554244721795982
6	42	0.21105775317676745
6	43	0.19705168925803207
6	44	0.28217858099287696
6	45	-0.014115813640472652
6	46	0.003664781660638994
6	47	0.20826421923855776
6	48	0.2714497915108822
6	49	0.18110341241963823
6	50	0.21961354856010473
7	1	-0.15458674630042424
7	2	-0.0025286092649565717
7	3	-0.23075047748417887
7	4	-0.3307209841293286
7	5	-0.3291947257915524
7	6	-0.10713058179820933
7	7	-0.3030156752612072
7	8	-0.2119786148227405
7	9	-0.1254877209632852
7	10	-1.1380293747204533
7	11	-0.2297282553073856
7	12	-0.16479094040374775
7	13	-0.13426757507820497
7	14	-0.12986884258328502
7	15	-0.23884741563111334
7	16	-0.08394935688961169
7	17	-0.09149522776704799
7	18	0.04444028087236518
7	19	-0.01428281937404563
7	20	-0.041689079220354586
7	21	-0.031091103201610792
7	22	-0.15317465131741415
7	23	-0.03450805426746939
7	24	-0.05176545787873721
7	25	-0.5051363878764192
7	26	-0.08736337815129502
7	27	-0.253123722573406
7	28	-0.649926269106146
7	29	-0.19384765529356685
7	30	-0.5964959415102697
7	31	-0.19706917275405544
7	32	-0.19803050288782487
7	33	-0.14526373448370578
7	34	-0.16670432407985203
7	35	-0.24359064400687913
7	36	-0.20649068590108755
7	37	0.10410704776331414
7	38	0.07802354378971188
7	39	-0.5236700090421991
7	40	0.2746650037713607
7	41	0.14838006941614168
7	42	-0.011730520314237936
7	43	0.012409844673143766
7	44	0.13666802013822377
7	45	-0.07494055582665027
7	46	-0.2414746021574743
7	47	-0.313187930406265
7	48	0.07354206472875546
7	49	0.09671118352694918
7	50	-0.08608823799748677
8	1	-0.17607002408986006
8	2	-0.09582055183778238
8	3	-0.29205860350018753
8	4	-0.2588387226363196
8	5	-0.14159045686106708
8	6	-0.10265740238336463
8	7	-0.16186757187562506
8	8	-0.24691038030217527
8	9	0.0033415431766670167
8	10	0.020708338002144444
8	11	0.10699546593045284
8	12	-0.21202005145470793
8	13	-0.04456501215605613
8	14	-0.03055095979017608
8	15	-0.10708379512797706
8	16	-0.07677301607940734
8	17	-0.06414287287945086
8	18	0.04938563499052151
8	19	-0.08650121278031264
8	20	-0.013956656852300853
8	21	-0.16838825486767917
8	22	-0.10978347688124046
8	23	-0.12718040284985221
8	24	-0.21175904084399377
8	25	-0.17583152138130487
8	26	-0.12062649767943867
8	27	-0.14828120269844192
8	28	-0.2134798160511835
8	29	-0.20425868644971956
8	30	-0.28807739461270643
8	31	-0.4054167570730556
8	32	-0.9580432969829573
8	33	-0.3154342258779721
8	34	-0.2630273775771812
8	35	-0.2713797933097979
8	36	-0.304270116406812
8	37	-1.2428659022464643
8	38	-0.09204689665046573
8	39	-0.12760413512241087
8	40	0.16932291099126928
8	41	0.08669080624341063
8	42	-0.09328630314687558
8	43	-0.1109702520934377
8	44	-0.14516868610169098
8	45	-0.15267253370373624
8	46	-0.2185821217872217
8	47	-0.15592946701265475
8	48	-0.4678661472721828
8	49	-0.11181367938871034
8	50	-0.5514383705665935
9	1	-0.13380962680016495
9	2	-0.061278748009158335
9	3	-0.16392254180411214
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115	45	0.48542302756361266
115	46	0.5304360868862901
115	47	0.5118479867271546
115	48	0.5869987787655262
115	49	0.904248549068221
115	50	0.6642413839776822
116	1	-0.0752559696076176
116	2	0.006051614942464312
116	3	-0.11291312184660285
116	4	-0.08791175704823928
116	5	-0.0899070457343143
116	6	0.022463948433447456
116	7	-0.040699720690930974
116	8	-0.024266508226759242
116	9	0.07443997227553467
116	10	0.18129044283998041
116	11	0.03883108109971545
116	12	-0.14107645622668485
116	13	0.08767138867832358
116	14	-0.12822649457532975
116	15	0.0193005802984203
116	16	-0.1654436717019081
116	17	0.01481234064527115
116	18	0.16479846922224084
116	19	0.09883816724656214
116	20	0.12222940396965498
116	21	0.0813522091597676
116	22	-9.95804686823476E-4
116	23	0.057076362532029634
116	24	0.0437731916058226
116	25	-0.2813930173783561
116	26	0.1095321381778831
116	27	-0.2530678606852135
116	28	0.07236121763592962
116	29	-0.235499656772852
116	30	0.137378980367572
116	31	0.06516575814729464
116	32	0.23494316996016806
116	33	-0.3934456088323657
116	34	0.2722709535319865
116	35	0.18183340788364433
116	36	0.1927091827322016
116	37	0.560570892195777
116	38	0.1358685714912582
116	39	0.6429919950426211
116	40	0.9655855347354034
116	41	0.43159797988328563
116	42	0.4992206568036508
116	43	0.18145097240973485
116	44	0.20748109894426392
116	45	0.5367555693339128
116	46	0.05458843363122412
116	47	0.6761470124218718
116	48	0.8220367965233919
116	49	0.3757150074939659
116	50	0.6937402983489207
117	1	-0.14218599550545719
117	2	-0.027101273368458578
117	3	-0.14601985099539405
117	4	-0.14901305836703926
117	5	-0.10240159018619721
117	6	-0.037304581509779666
117	7	-0.12351861006965237
117	8	-0.1007981965927911
117	9	-0.08222131127269705
117	10	0.019692438290874748
117	11	-0.03819351848255792
117	12	0.05169077958877466
117	13	-0.07742258787057921
117	14	0.03872512129009209
117	15	0.004733929900062606
117	16	-0.02124796602436163
117	17	0.06542137025944328
117	18	0.025053517250420043
117	19	0.013475553940914153
117	20	0.06618660972628732
117	21	0.04085625083543931
117	22	0.06070637257234068
117	23	-0.14712444375675204
117	24	-0.3163092607334477
117	25	0.0664013334714113
117	26	0.07742520961341626
117	27	-0.12455455865513443
117	28	-0.03047378576815163
117	29	-0.09374560126789078
117	30	0.04758025047097192
117	31	0.055938355856760325
117	32	0.0801633625214464
117	33	0.11149682251893722
117	34	0.22616215045616883
117	35	0.16247082034106342
117	36	0.3323723935286651
117	37	0.46572014759644986
117	38	0.5926276227092941
117	39	0.562757030299025
117	40	0.8791545519817134
117	41	0.37887733334220286
117	42	0.6787893941495042
117	43	0.4662331167850553
117	44	0.7517801814157963
117	45	0.6091408843292072
117	46	0.34111042571505124
117	47	0.19602912574058706
117	48	0.6415561209784144
117	49	0.6615495930718822
117	50	0.07195194819023243
118	1	-0.17561129250826468
118	2	-0.061400578187786437
118	3	-0.22346307146924715
118	4	-0.15896082191908079
118	5	-0.15187348009390433
118	6	-0.29171939593062746
118	7	-0.366767144208481
118	8	-0.15738071875246362
118	9	0.0016260076448730842
118	10	0.1053366603616368
118	11	-0.043417939552149676
118	12	-0.022117619287776336
118	13	-0.08912174537732653
118	14	-0.12373872450330481
118	15	-0.3457957435907204
118	16	-0.023948203785217004
118	17	0.025207697381866012
118	18	0.040296723563919556
118	19	0.020655784550974943
118	20	0.02399783881770645
118	21	-0.256502969864961
118	22	-0.053406167183602804
118	23	-0.04395664035964586
118	24	-0.03848624391331157
118	25	-0.20673490762266766
118	26	-0.13645972200866652
118	27	-0.16933570663783115
118	28	-0.12635729737066725
118	29	-0.26251649544759204
118	30	-0.5009242340956774
118	31	-0.3422991209612931
118	32	-0.025042994144378383
118	33	-0.5647444216850772
118	34	0.09083182033407411
118	35	-0.4468662156459615
118	36	0.1420506488808968
118	37	0.3539582973169999
118	38	0.5369539568637549
118	39	0.43843764177663047
118	40	0.8009343895307026
118	41	0.676292659827844
118	42	0.5679056865550152
118	43	0.47636229571716626
118	44	0.0545391388458043
118	45	0.05665795994508471
118	46	0.2702127927279889
118	47	0.37893686287839756
118	48	0.5762248415725892
118	49	0.26224597514682557
118	50	0.5455942914604179
119	1	-0.17501464324203653
119	2	-0.0871426536086517
119	3	-0.20448856400449245
119	4	-0.1993527649579363
119	5	-0.1801311692260299
119	6	-0.1009375365982379
119	7	-0.4263008016956533
119	8	-0.12099355811967882
119	9	0.0038507581556928017
119	10	-0.26955605530579874
119	11	-0.015004602914501675
119	12	-0.08271783194256699
119	13	-0.19445219248803625
119	14	-0.24646586824687233
119	15	-0.17345479730560953
119	16	-0.1464015606724942
119	17	-0.035415176800420056
119	18	0.046005540361491626
119	19	-0.04542212959839276
119	20	-0.09650387916330772
119	21	-0.8998189390635787
119	22	-0.07013170121415868
119	23	-0.11062411759242252
119	24	-0.15313721846889194
119	25	-0.24682075370269985
119	26	-0.38680612317928365
119	27	-0.19704001013992212
119	28	-0.2770804276238579
119	29	-0.5289890150286496
119	30	-0.08441315882857481
119	31	-0.5074721928329282
119	32	-0.2737201740119346
119	33	0.04614302840496265
119	34	0.08674240571693304
119	35	0.07633746374332162
119	36	-0.16324126135576478
119	37	0.2008438818508438
119	38	-0.48751684132043493
119	39	0.15228138555443138
119	40	0.8088696489288552
119	41	0.6052626791457882
119	42	0.43175461452357666
119	43	0.37720865545188786
119	44	0.5534887471453374
119	45	0.32662339141262464
119	46	0.1198927125136251
119	47	0.22432734105662888
119	48	0.47742007972110656
119	49	0.5590370967152083
119	50	0.4511415735870301
120	1	-0.23667517918875092
120	2	-0.17800076991244396
120	3	-0.34756027181582994
120	4	-0.5596637012157046
120	5	-0.2027780086551516
120	6	-0.41172946716044123
120	7	-0.18896029737913977
120	8	-0.18751533682372212
120	9	-0.03205374271917805
120	10	0.12198374691004688
120	11	-0.040931449227706196
120	12	-0.10005662120097725
120	13	-0.2230030647779131
120	14	-0.2164954425656802
120	15	-0.30100612260532955
120	16	-0.0619867060037933
120	17	0.0015636688621398775
120	18	0.07221337400363836
120	19	-0.0011017104405794953
120	20	-0.065955025792924
120	21	-0.11513787379190887
120	22	-0.1074001641474922
120	23	-0.11567622167359559
120	24	-0.16087110305859653
120	25	-0.22021399814249776
120	26	-0.37567317427996727
120	27	-0.3244717642916086
120	28	-0.2842270091483883
120	29	-0.4667293435069908
120	30	-0.333050451763377
120	31	-0.5705162043438889
120	32	-0.28044338559436
120	33	-1.5098595825374872
120	34	-0.044513354657453874
120	35	-0.07836747422294366
120	36	-0.3797183968788431
120	37	-0.15316173272869804
120	38	-1.6944064026083723
120	39	0.015435580108601243
120	40	-0.21658055531310083
120	41	0.24566457012155496
120	42	0.1740466406262442
120	43	-0.5122261355251609
120	44	0.20517441077991805
120	45	0.015918760884929384
120	46	0.11608472509257695
120	47	0.18769839109449293
120	48	0.5279083188045774
120	49	0.556171875539782
120	50	0.3898391270655921
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	25195.0
3	1710.0
4	1259.0
5	1753.0
6	2669.0
7	4142.0
8	5022.0
9	6815.0
10	9184.0
11	12067.0
12	15717.0
13	20493.0
14	25418.0
15	30145.0
16	34161.0
17	38293.0
18	42987.0
19	48705.0
20	54008.0
21	61521.0
22	72538.0
23	89483.0
24	113287.0
25	151195.0
26	206388.0
27	279174.0
28	367680.0
29	467882.0
30	584379.0
31	724999.0
32	907497.0
33	1170615.0
34	1569020.0
35	2174637.0
36	3100016.0
37	4666479.0
38	6106087.0
39	1.2102709E7
40	16741.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	15.115870663989003	28.149217386390053	29.621360418174685	27.113551531446255
2	23.240166138636035	31.667902045196673	24.74695224366622	20.344979572501067
3	23.0580345638289	28.531753740789974	26.59120708335906	21.81900461202207
4	24.33392412200482	26.359442397821336	25.459102551558548	23.847530928615292
5	26.065310464156035	28.055399777463645	26.65575592285486	19.223533835525465
6	26.17769463333186	27.008459437630904	23.142163409420696	23.67168251961654
7	23.899221994066053	27.504626605010696	27.26592918511999	21.330222215803264
8	23.954072927472108	28.09381608045068	25.852585249179672	22.09952574289754
9	24.034609129399666	26.168474405493647	26.837792290284877	22.95912417482181
10	22.690694711468346	28.784497765211725	25.26702626042597	23.25778126289396
11	24.315255378684967	26.910676717620913	25.68612092126007	23.08794698243405
12	22.793659505092734	26.485657736858816	26.532335827381402	24.18834693066705
13	24.06916105456293	26.52280367591025	26.33589308131752	23.072142188209302
14	24.246550825256065	26.95642311538236	26.373036188476064	22.423989870885507
15	22.512191798965436	26.944915726659595	27.711331005157813	22.831561469217156
16	22.47855607299721	26.68081276328353	27.902293439855068	22.938337723864187
17	22.989921924272824	26.426357227751396	26.636475607059236	23.94724524091655
18	22.411204067146063	27.772195167233924	26.638372063189564	23.178228702430452
19	24.002003394363552	26.786464990199242	26.599596868707977	22.61193474672923
20	23.050996875996915	26.36616408584431	26.704699937542593	23.878139100616178
21	22.662200846968215	27.97890715494467	26.566095574858554	22.792796423228555
22	24.157548856134373	26.354350268918303	26.566290513323576	22.921810361623752
23	24.032358852680304	26.412232145633414	26.87617633504027	22.679232666646012
24	22.66047862676058	27.85085223982233	26.536794781253413	22.951874352163674
25	22.883651942639364	27.57924036447086	26.442480022413605	23.09462767047617
26	23.149917806664156	26.457913163018947	27.854530045041002	22.537638985275894
27	24.28092357393904	26.385361899874393	26.5992107506581	22.734503775528466
28	23.08237753064511	26.402779011817216	26.42857039678655	24.086273060751118
29	23.1989741562629	26.411595865876702	26.49348288541182	23.895947092448576
30	24.289710473015184	26.374295586457368	26.499437483194527	22.836556457332918
31	22.99268886172436	27.62992862786719	26.52491288595557	22.85246962445288
32	24.341805117381238	26.386598456370532	26.470854268039997	22.80074215820824
33	23.01946197415152	27.65728730621558	26.39321626825061	22.93003445138229
34	23.076562376131697	26.452016291924117	27.545559310558552	22.92586202138563
35	23.094737594915877	26.455422122624807	26.502727053467524	23.947113228991793
36	24.09290686129309	26.383430494701432	26.62132154720574	22.902341096799734
37	24.00831041832006	26.542046459914413	26.505820731563134	22.943822390202392
38	23.06852489326711	27.705895895947208	26.55645670792861	22.66912250285707
39	22.91054669625748	27.52195457740439	26.55455780065748	23.01294092568065
40	23.769873640037563	26.39519655929648	26.884347885413234	22.95058191525272
41	23.080935286535702	27.65686231031151	26.43671686512073	22.82548553803206
42	24.28778980888707	26.36800523311667	26.428932886088713	22.915272071907548
43	23.043102478974493	26.370032164794438	26.55344166473343	24.033423691497642
44	24.14416489173432	26.615581331380028	26.415285887737987	22.82496788914766
45	24.035357383830377	26.45329974329165	26.586487953499848	22.924854919378117
46	23.075605164591074	26.36876988443282	27.669161350171407	22.886463600804692
47	23.19236684824023	26.40019642115175	27.51614866274523	22.891288067862785
48	22.94395832630771	26.383109141511625	26.371320261089693	24.30161227109097
49	22.965898807806166	27.441105201690082	26.499367087943988	23.093628902559768
50	24.236096936328487	26.388409154263297	26.483541841138695	22.891952068269514
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2065.0
1	1260.0
2	455.0
3	391.5
4	328.0
5	372.5
6	417.0
7	762.5
8	1108.0
9	1669.5
10	2231.0
11	3755.5
12	5280.0
13	8801.5
14	12323.0
15	20063.0
16	27803.0
17	44843.0
18	61883.0
19	91101.5
20	120320.0
21	167322.0
22	214324.0
23	301369.0
24	388414.0
25	426625.5
26	464837.0
27	554638.5
28	644440.0
29	737237.0
30	830034.0
31	953472.5
32	1076911.0
33	1208136.5
34	1339362.0
35	1494977.0
36	1650592.0
37	1781757.0
38	1912922.0
39	2034641.0
40	2156360.0
41	2246074.0
42	2335788.0
43	2414180.5
44	2492573.0
45	2516217.0
46	2539861.0
47	2495340.0
48	2450819.0
49	2408011.5
50	2365204.0
51	2308186.0
52	2251168.0
53	2156287.5
54	2061407.0
55	1948816.0
56	1836225.0
57	1890258.5
58	1944292.0
59	1648817.0
60	1353342.0
61	1166738.5
62	980135.0
63	830359.5
64	680584.0
65	572329.5
66	464075.0
67	378330.5
68	292586.0
69	231481.5
70	170377.0
71	132147.5
72	93918.0
73	70808.0
74	47698.0
75	34823.5
76	21949.0
77	16184.0
78	10419.0
79	7202.5
80	3986.0
81	2762.5
82	1539.0
83	1222.0
84	905.0
85	663.5
86	422.0
87	299.5
88	177.0
89	124.5
90	72.0
91	48.0
92	24.0
93	22.5
94	21.0
95	16.5
96	12.0
97	11.5
98	11.0
99	41.5
100	72.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.18239655732445026
2	0.0010081538692010975
3	2.8318928910143188E-5
4	0.0014584248388723744
5	2.4637468151824575E-4
6	8.495678673042956E-5
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	3.6814607583186146E-5
16	2.57702253082303E-4
17	2.0106439526201662E-4
18	1.0194814407651548E-4
19	2.8318928910143188E-6
20	1.4159464455071594E-5
21	2.8318928910143188E-6
22	6.513353649332933E-4
23	1.812411450249164E-4
24	8.382402957402384E-4
25	0.00388818893936266
26	0.012638737972596906
27	0.015170450217163707
28	0.01616161272901872
29	0.017297201778315462
30	0.0208059170702822
31	0.026891654893071975
32	0.03183330798789196
33	0.0402468617670955
34	0.04963741859369898
35	0.05597519488378903
36	0.036016013787920104
37	0.023878520857032737
38	0.011522972173537264
39	0.006974952190568268
40	0.00721283119341347
41	0.001767101163992935
42	0.013086177049377166
43	0.03657956047323196
44	0.05297338841931385
45	0.0585012433425738
46	0.0655809755701096
47	0.06974385811990065
48	0.06960792726113195
49	0.06682417654926488
50	0.06644470290186896
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	3.531207E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.06934959652059
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.48590960532833	31.41226560789101
2	11.54591853234706	9.483667292358145
3	4.124902165150115	5.082211472159845
4	2.0307799244066933	3.336112426762166
5	1.2215807485969932	2.508476341225462
6	0.728299612848082	1.7946474846641085
7	0.5512769577068686	1.584841027039878
8	0.4029670354421078	1.3239675243556344
9	0.3126966395616083	1.1558022847031595
>10	2.1155450804733693	17.267591748175395
>50	0.2944797029530869	8.430386840328994
>100	0.1785511312691746	13.203029985525053
>500	0.006187096597605844	1.651871786358623
>1k	8.781796530103222E-4	0.4946075630786695
>5k	0.0	0.0
>10k+	2.7587665905610603E-5	1.2705206153740902
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAG	331904	0.9399165780992165	Illumina Paired End PCR Primer 2 (97% over 36bp)
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	55581	0.15739943877546686	No Hit
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGT	36306	0.10281470330116586	Illumina Paired End PCR Primer 2 (100% over 50bp)
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.001696303841717577	0.0	0.0	2.8318928910143188E-6	0.0
2	0.0017246227706277201	5.6637857820286375E-6	0.0	5.6637857820286375E-6	0.0
3	0.001730286556409749	8.495678673042956E-6	0.0	5.6637857820286375E-6	0.0
4	0.001738782235082792	8.495678673042956E-6	0.0	5.6637857820286375E-6	0.0
5	0.001750109806646849	8.495678673042956E-6	0.0	5.6637857820286375E-6	0.0
6	0.0017529416995378634	8.495678673042956E-6	0.0	8.495678673042956E-6	0.0
7	0.0017699330568839494	8.495678673042956E-6	0.0	8.495678673042956E-6	0.0
8	0.002004980166838138	8.495678673042956E-6	0.0	8.495678673042956E-6	0.0
9	0.0025883501023870876	8.495678673042956E-6	0.0	8.495678673042956E-6	0.0
10	0.004505541589603782	8.495678673042956E-6	0.0	8.495678673042956E-6	0.0
11	0.004681118948846669	8.495678673042956E-6	0.0	8.495678673042956E-6	0.0
12	0.004896342808563757	8.495678673042956E-6	0.0	8.495678673042956E-6	0.0
13	0.004984131488185202	8.495678673042956E-6	0.0	1.1327571564057275E-5	0.0
14	0.005066256382024617	8.495678673042956E-6	0.0	1.4159464455071594E-5	0.0
15	0.008535325173517157	8.495678673042956E-6	0.0	1.6991357346085913E-5	2.8318928910143188E-6
16	0.008696743068304973	8.495678673042956E-6	0.0	1.6991357346085913E-5	2.8318928910143188E-6
17	0.009243298396270737	1.1327571564057275E-5	0.0	1.6991357346085913E-5	2.8318928910143188E-6
18	0.009716224509070129	1.1327571564057275E-5	0.0	1.6991357346085913E-5	2.8318928910143188E-6
19	0.010152336014286334	1.1327571564057275E-5	0.0	1.6991357346085913E-5	2.8318928910143188E-6
20	0.08986162521766637	1.1327571564057275E-5	0.0	1.6991357346085913E-5	2.8318928910143188E-6
21	0.09257457860725808	1.1327571564057275E-5	0.0	1.6991357346085913E-5	2.8318928910143188E-6
22	0.09310414257787776	1.1327571564057275E-5	0.0	1.9823250237100233E-5	2.8318928910143188E-6
23	0.09363937033427946	1.1327571564057275E-5	0.0	1.9823250237100233E-5	2.8318928910143188E-6
24	0.09596718629069324	1.1327571564057275E-5	0.0	1.9823250237100233E-5	2.8318928910143188E-6
25	0.09646559943951176	1.1327571564057275E-5	0.0	1.9823250237100233E-5	2.8318928910143188E-6
26	0.09771446420444907	1.1327571564057275E-5	0.0	2.265514312811455E-5	2.8318928910143188E-6
27	0.0982525238537418	1.1327571564057275E-5	0.0	2.548703601912887E-5	2.8318928910143188E-6
28	0.10045856841584194	1.1327571564057275E-5	0.0	2.548703601912887E-5	2.8318928910143188E-6
29	0.10249469940448125	1.1327571564057275E-5	0.0	2.548703601912887E-5	2.8318928910143188E-6
30	0.11891118249369124	1.1327571564057275E-5	0.0	2.8318928910143188E-5	2.8318928910143188E-6
31	1.1680991796855862	1.4159464455071594E-5	0.0	3.1150821801157505E-5	2.8318928910143188E-6
32	1.1773849564752221	1.4159464455071594E-5	0.0	3.3982714692171825E-5	2.8318928910143188E-6
33	1.182258644140658	1.4159464455071594E-5	0.0	3.3982714692171825E-5	2.8318928910143188E-6
34	1.1827089151103292	1.4159464455071594E-5	0.0	3.6814607583186146E-5	2.8318928910143188E-6
35	1.1832299834022757	1.4159464455071594E-5	0.0	3.9646500474200466E-5	2.8318928910143188E-6
36	1.1837765387302415	1.4159464455071594E-5	0.0	5.097407203825774E-5	2.8318928910143188E-6
37	1.1844193784165018	1.6991357346085913E-5	0.0	5.097407203825774E-5	2.8318928910143188E-6
38	1.1851585024610565	1.6991357346085913E-5	0.0	5.380596492927206E-5	2.8318928910143188E-6
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATGCCGA	51125	0.0	37.82105	25
TGCCGAG	52725	0.0	36.61657	26
AGATCGG	46355	0.0	36.221577	31
AATGCCG	53805	0.0	36.066055	24
CGAGATC	48800	0.0	35.402966	29
CCGAGAT	49830	0.0	34.77862	28
GAGATCG	49730	0.0	34.458035	30
GCCGAGA	57865	0.0	33.593903	27
TATGCCG	5645	0.0	32.63443	43
TCGTATG	5775	0.0	31.821905	40
GTTCAGC	63650	0.0	30.94855	14
GACCGAT	6020	0.0	30.340508	32
CAGGAAT	66185	0.0	29.916803	20
CTCGTAT	6195	0.0	29.804821	39
GGAATGC	66285	0.0	29.72206	22
GAATGCC	65780	0.0	29.706661	23
AGGAATG	67670	0.0	29.203022	21
CGTATGC	6385	0.0	29.023575	41
ACCGATC	6390	0.0	28.859291	33
GCAGGAA	71030	0.0	28.087326	19
>>END_MODULE