FastQCFastQC Report
Thu 26 May 2016
SRR522106_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR522106_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36937230
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1169110.31651263508389776No Hit
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCG1011260.2737779741469515Illumina Paired End PCR Primer 2 (100% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC421800.030.97938712
ATCAACG431500.030.3899111
CAACGCA435700.029.86498313
AAGCAGT465450.029.2734221
GTATCAA453350.029.0318439
AACGCAG447950.028.96476714
ACGCAGA445550.028.90376315
GGTATCA461250.028.6107868
GTGGTAT467000.028.2872036
CGCAGAG462900.027.75416
TGGTATC475600.027.7382557
AGTGGTA489200.027.115855
TATCAAC487350.026.98389210
AGAGTAC467450.026.63304519
AGCAGTG531150.025.6343732
CAGAGTA492350.025.51922818
GCAGAGT503300.025.27361917
GTACTTT281850.023.51569222
CAGTGGT572500.023.4360854
ATGCCGA208000.023.35635414
Produced by FastQC (version 0.11.5)