##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR522106_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	36937230
Sequences flagged as poor quality	0
Sequence length	50
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.81430543113276	40.0	39.0	40.0	36.0	40.0
2	38.686731625517126	40.0	39.0	40.0	36.0	40.0
3	38.67435070252967	40.0	39.0	40.0	36.0	40.0
4	38.669716029057945	40.0	39.0	40.0	36.0	40.0
5	38.673927010769354	40.0	39.0	40.0	36.0	40.0
6	38.71434322497924	40.0	39.0	40.0	36.0	40.0
7	38.68249089062715	40.0	39.0	40.0	36.0	40.0
8	38.66390051446738	40.0	39.0	40.0	36.0	40.0
9	38.65292754762607	40.0	39.0	40.0	36.0	40.0
10	38.63960657038982	40.0	39.0	40.0	36.0	40.0
11	38.67902492959001	40.0	39.0	40.0	36.0	40.0
12	38.638330324174284	40.0	39.0	40.0	36.0	40.0
13	38.617833416311946	40.0	39.0	40.0	36.0	40.0
14	38.5974985130179	40.0	39.0	40.0	36.0	40.0
15	38.581050094985464	40.0	39.0	40.0	36.0	40.0
16	38.60017903345757	40.0	39.0	40.0	36.0	40.0
17	38.55139340443233	40.0	39.0	40.0	36.0	40.0
18	38.51970302050262	40.0	39.0	40.0	35.0	40.0
19	38.48786868966623	40.0	39.0	40.0	35.0	40.0
20	38.450962051025485	40.0	38.0	40.0	35.0	40.0
21	38.44496715102892	40.0	38.0	40.0	35.0	40.0
22	38.39897799591361	40.0	38.0	40.0	35.0	40.0
23	38.34706644217771	40.0	38.0	40.0	35.0	40.0
24	38.32135988540559	40.0	38.0	40.0	35.0	40.0
25	38.28092772522466	40.0	38.0	40.0	35.0	40.0
26	38.19847622574839	40.0	38.0	40.0	35.0	40.0
27	38.13465232774629	40.0	38.0	40.0	35.0	40.0
28	38.082340392065134	40.0	38.0	40.0	35.0	40.0
29	38.02861928195482	40.0	38.0	40.0	35.0	40.0
30	37.969831062047696	40.0	38.0	40.0	35.0	40.0
31	37.89193342326969	40.0	38.0	40.0	35.0	40.0
32	37.817236863728006	40.0	38.0	40.0	34.0	40.0
33	37.76152042261967	40.0	38.0	40.0	33.0	40.0
34	37.70087323819355	39.0	38.0	40.0	33.0	40.0
35	37.628781069939464	39.0	38.0	40.0	33.0	40.0
36	37.53629254278136	39.0	38.0	40.0	33.0	40.0
37	37.43721987274086	39.0	38.0	40.0	33.0	40.0
38	37.351740885821705	39.0	38.0	40.0	33.0	40.0
39	37.68878762700939	40.0	38.0	40.0	33.0	40.0
40	37.6510810366668	40.0	38.0	40.0	33.0	40.0
41	37.84631614227705	40.0	38.0	40.0	35.0	40.0
42	37.7788231277765	40.0	38.0	40.0	35.0	40.0
43	37.72106408628909	40.0	38.0	40.0	34.0	40.0
44	37.66351404802147	40.0	38.0	40.0	34.0	40.0
45	37.60900468172627	40.0	38.0	40.0	34.0	40.0
46	37.58298510743767	40.0	38.0	40.0	34.0	40.0
47	37.50090291015325	40.0	38.0	40.0	33.0	40.0
48	37.40806435674792	40.0	38.0	40.0	33.0	40.0
49	37.34655227259868	40.0	38.0	40.0	33.0	40.0
50	37.269288682448575	40.0	38.0	40.0	33.0	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1	1	-0.1639768321180881
1	2	-0.1853689233161191
1	3	-0.18555907367309032
1	4	-0.173036197478865
1	5	-0.1749520879368518
1	6	-0.17620735719110314
1	7	-0.19328982967697783
1	8	-0.17891285822948788
1	9	-0.19907698302643695
1	10	-0.19656332002368515
1	11	-0.20310735096607146
1	12	-0.22290424625898453
1	13	-0.2159970590910092
1	14	-0.21442417704670902
1	15	-0.20200660025355432
1	16	-0.2112737331943606
1	17	-0.18059029174398944
1	18	-0.20299835006418476
1	19	-0.19500278618566824
1	20	-0.16700754380487126
1	21	-0.19419682257516513
1	22	-0.18249178555554835
1	23	-0.18433003030256145
1	24	-0.1901323680981335
1	25	-0.18103187859700398
1	26	-0.17176337720209744
1	27	-0.18510263721716314
1	28	-0.15670988542566988
1	29	-0.16793279063646338
1	30	-0.1728011125676545
1	31	-0.1792180584174119
1	32	-0.17997444925935469
1	33	-0.18128837711691403
1	34	-0.18480882150068823
1	35	-0.17328678962062583
1	36	-0.1596098836501625
1	37	-0.1911528539583216
1	38	-0.16910012059145885
1	39	-0.15314660396589375
1	40	-0.16509518601976225
1	41	-0.10858209091136217
1	42	-0.12350559212735845
1	43	-0.12118176716256102
1	44	-0.11079212067392774
1	45	-0.13424629598070936
1	46	-0.10432071081019245
1	47	-0.10049756833004864
1	48	-0.07662088501779607
1	49	-0.06483956803895552
1	50	-0.11870370182337098
2	1	-0.14805021963844922
2	2	-0.15995664355644834
2	3	-0.16880567854422424
2	4	-0.1625866669654954
2	5	-0.15632374061836884
2	6	-0.17144030338020855
2	7	-0.19196699864401268
2	8	-0.1701660192314165
2	9	-0.19899554447243872
2	10	-0.18866213020967848
2	11	-0.17939033903513746
2	12	-0.16905035874437147
2	13	-0.19812093449899493
2	14	-0.1663648223184495
2	15	-0.18279322799826048
2	16	-0.16607375357445875
2	17	-0.160450903637539
2	18	-0.18296248602067777
2	19	-0.1583043309098855
2	20	-0.1554412844069617
2	21	-0.18230796332395727
2	22	-0.19510931584204627
2	23	-0.20526005345342924
2	24	-0.18844016351341963
2	25	-0.17255125024901474
2	26	-0.15892908719617083
2	27	-0.1672476107390537
2	28	-0.1456299561911507
2	29	-0.1620800495869048
2	30	-0.18484605810087373
2	31	-0.14779693144573258
2	32	-0.16244296032849803
2	33	-0.17903203530799772
2	34	-0.17933484419602053
2	35	-0.190645635178754
2	36	-0.20062443649793238
2	37	-0.17024872010670578
2	38	-0.18561704123407452
2	39	-0.15287355941554637
2	40	-0.14853472721983252
2	41	-0.1388355089308959
2	42	-0.1440252267279689
2	43	-0.16573973788602814
2	44	-0.23625507931258483
2	45	-0.16724523053175488
2	46	-0.1533130627364443
2	47	-0.14956050574832602
2	48	-0.15431813458152277
2	49	-0.18408418273254057
2	50	-0.17814501932723914
3	1	-0.12180860631271173
3	2	-0.14713388015168505
3	3	-0.16300474985828117
3	4	-0.13687444998271303
3	5	-0.14566754471943
3	6	-0.1652186084391971
3	7	-0.15471987534827747
3	8	-0.15094444322378564
3	9	-0.16405443716226387
3	10	-0.16511601317136382
3	11	-0.15711694094277107
3	12	-0.1640921348600628
3	13	-0.16834315804183575
3	14	-0.1750952244800672
3	15	-0.1862111641755959
3	16	-0.16941657500385787
3	17	-0.17109035711306575
3	18	-0.14740187516314762
3	19	-0.15235748411807037
3	20	-0.16070011974869658
3	21	-0.16569078367850665
3	22	-0.156422725297368
3	23	-0.13705516775644355
3	24	-0.15049701415613725
3	25	-0.15321509968686087
3	26	-0.12016005846974309
3	27	-0.123484981013263
3	28	-0.13143883890887764
3	29	-0.13455152276237925
3	30	-0.15959959998908602
3	31	-0.1421620785198101
3	32	-0.14827608169785833
3	33	-0.15086635957720063
3	34	-0.16829247311189022
3	35	-0.12204240584293302
3	36	-0.15383370495580806
3	37	-0.1352948243158778
3	38	-0.12690416771044966
3	39	-0.16916187232885704
3	40	-0.15168295334067494
3	41	-0.13728087420543034
3	42	-0.13201479865966093
3	43	-0.14600658199471184
3	44	-0.1503463895252466
3	45	-0.14195528582593653
3	46	-0.1675990526219877
3	47	-0.14484821405398662
3	48	-0.15229019224087637
3	49	-0.1681803915990585
3	50	-0.17195876587566516
4	1	-0.13760524886563985
4	2	-0.1285560039119673
4	3	-0.14110557536633905
4	4	-0.1308312390034203
4	5	-0.14380110312811922
4	6	-0.1349575773129814
4	7	-0.1408480260496603
4	8	-0.15140072694967444
4	9	-0.1516429708082967
4	10	-0.135367186007052
4	11	-0.11937752019232306
4	12	-0.13879804639884696
4	13	-0.14844613529902517
4	14	-0.13432056001961712
4	15	-0.12534754760433486
4	16	-0.14196346984231667
4	17	-0.13787350171192259
4	18	-0.15303973745178467
4	19	-0.12735390724083118
4	20	-0.12451833557615544
4	21	-0.10701522996870239
4	22	-0.12154178730570209
4	23	-0.12889350406788225
4	24	-0.14040684028341133
4	25	-0.11442626660343791
4	26	-0.11912193478185884
4	27	-0.14569124335561412
4	28	-0.12891112068856359
4	29	-0.14198643102781716
4	30	-0.12132140103766886
4	31	-0.13996122783433407
4	32	-0.1347476374286316
4	33	-0.14365046326113173
4	34	-0.15569746159120967
4	35	-0.1404221449075962
4	36	-0.10682143856517001
4	37	-0.1280497103808571
4	38	-0.1466808761738676
4	39	-0.14101163948335937
4	40	-0.1380261784927228
4	41	-0.12464047633478259
4	42	-0.12625450083960033
4	43	-0.16762482500072906
4	44	-0.13417581814277924
4	45	-0.1338106292023511
4	46	-0.11051575618439813
4	47	-0.12022437144747755
4	48	-0.12322984663370562
4	49	-0.12087928598440101
4	50	-0.12494493999430034
5	1	-0.06108090550858236
5	2	-0.08251475001065955
5	3	-0.09025423178010072
5	4	-0.07913005817311358
5	5	-0.07947940112234875
5	6	-0.07263259100808028
5	7	-0.08330197732714595
5	8	-0.07397889546157188
5	9	-0.09690559565133583
5	10	-0.0758054779506736
5	11	-0.08181170447491581
5	12	-0.06426615997420981
5	13	-0.06999765669673508
5	14	-0.062368517222466835
5	15	-0.05793115629859358
5	16	-0.05867765332362751
5	17	-0.05508619770004941
5	18	-0.05664946170283258
5	19	-0.041940567737015044
5	20	-0.04719514189730489
5	21	-0.05946106434898013
5	22	-0.05834228655955087
5	23	-0.04074457619706351
5	24	-0.04473546830853792
5	25	-0.04906760179990499
5	26	-0.026536671477188634
5	27	-0.03144345464226461
5	28	-0.06137349644096446
5	29	-0.01461308640716652
5	30	-0.03030729205325855
5	31	-0.007905682767962219
5	32	0.027898933213847954
5	33	-0.0048371438523915344
5	34	-0.018533287171614177
5	35	-0.028368923236023136
5	36	0.013870513513907667
5	37	0.05261899039980733
5	38	0.027823927638081614
5	39	0.05846328591326966
5	40	0.06999896590713206
5	41	0.04737968309773777
5	42	0.025606935870136738
5	43	0.02304541209122135
5	44	0.04220086424820835
5	45	0.06451977583333957
5	46	0.07015374903030391
5	47	0.06994651715717737
5	48	0.08982930403221445
5	49	0.07083852928000312
5	50	0.09498337546877167
6	1	-0.07530196285016189
6	2	-0.10241433277474954
6	3	-0.10143978965712108
6	4	-0.08661302278655114
6	5	-0.11335358025505116
6	6	-0.0938018553777269
6	7	-0.10053319907851233
6	8	-0.085042323836646
6	9	-0.10626374613347878
6	10	-0.11751020304653537
6	11	-0.11170134433808698
6	12	-0.09891346250358879
6	13	-0.11054501833888253
6	14	-0.10160155863001563
6	15	-0.11034273793255522
6	16	-0.11447838625665696
6	17	-0.11287265641289679
6	18	-0.12487029125939841
6	19	-0.11829215035884744
6	20	-0.09392258503783069
6	21	-0.11351556843771249
6	22	-0.08707466934432517
6	23	-0.10004601267871038
6	24	-0.1046988872552106
6	25	-0.0857101347437208
6	26	-0.09567231989910852
6	27	-0.11012612469535554
6	28	-0.08586344009076896
6	29	-0.1162479757525503
6	30	-0.10534514305058451
6	31	-0.07936755017744446
6	32	-0.11136536835365263
6	33	-0.1179997969251474
6	34	-0.10495372263599734
6	35	-0.10724680086121907
6	36	-0.08124782033942779
6	37	-0.09504980515608707
6	38	-0.08603236519416413
6	39	-0.10176733010158756
6	40	-0.10600649625266811
6	41	-0.12182778993851429
6	42	-0.13046818510253644
6	43	-0.19057494028695032
6	44	-0.09714499172932989
6	45	-0.09134538862945618
6	46	-0.07747182623410964
6	47	-0.09334239632568853
6	48	-0.08577763718417941
6	49	-0.100046124381997
6	50	-0.08270914426500298
7	1	-0.07879772334246837
7	2	-0.06713531768159697
7	3	-0.09773240628985747
7	4	-0.08100882870889592
7	5	-0.08676213358865681
7	6	-0.09206476456024149
7	7	-0.0758813285597455
7	8	-0.08004851343460473
7	9	-0.09608444022455132
7	10	-0.08474843759744743
7	11	-0.08150104611654996
7	12	-0.08689347034727035
7	13	-0.09535947870978134
7	14	-0.07686406906432097
7	15	-0.07636647617275116
7	16	-0.07321694966920234
7	17	-0.07919161220753068
7	18	-0.08479415510539212
7	19	-0.07818057427862612
7	20	-0.0627495701575711
7	21	-0.0584220177211634
7	22	-0.056536976547256756
7	23	-0.07279089018872043
7	24	-0.07045624393069261
7	25	-0.06694322637201822
7	26	-0.06004635521603774
7	27	-0.07580324444218434
7	28	-0.05715761989506518
7	29	-0.061081007976369506
7	30	-0.08823650292577412
7	31	-0.08268156206008115
7	32	-0.07275169103039758
7	33	-0.062155949051252435
7	34	-0.09776015538405858
7	35	-0.06910556591559214
7	36	-0.06479101933678777
7	37	-0.07566793713861131
7	38	-0.055417179610984135
7	39	-0.06931980226379864
7	40	-0.07507684273015514
7	41	-0.05839608561480958
7	42	-0.04949595580352906
7	43	-0.06427306058061077
7	44	-0.04432107002998009
7	45	-0.06876138157284117
7	46	-0.026392115727368548
7	47	-0.052487873791264406
7	48	-0.04508187004403652
7	49	-0.04115244547782737
7	50	-0.03662880442290373
8	1	-0.04201471151793612
8	2	-0.04408688577195363
8	3	-0.058247274061258736
8	4	-0.03911330771114052
8	5	-0.05382080829873104
8	6	-0.06606197938405245
8	7	-0.05397714648702845
8	8	-0.07532687556051343
8	9	-0.06142113282265882
8	10	-0.06772220846342947
8	11	-0.059173719534854285
8	12	-0.050902786733843186
8	13	-0.07266132840143769
8	14	-0.06869769604568177
8	15	-0.07564518902390915
8	16	-0.0859448236219933
8	17	-0.08012445161057258
8	18	-0.0855115803783093
8	19	-0.06638290332580965
8	20	-0.06726019396733562
8	21	-0.059887425087552515
8	22	-0.05474107696638697
8	23	-0.06682573553884197
8	24	-0.06457884332025543
8	25	-0.07562369435050442
8	26	-0.05150213654757607
8	27	-0.042828362286854826
8	28	-0.035213593590206926
8	29	-0.043230021055428836
8	30	-0.04761245184905505
8	31	-0.0646006007913087
8	32	-0.053125579384683874
8	33	-0.049157034682032474
8	34	-0.07281049140944873
8	35	-0.040381758234509846
8	36	-0.03964441787196904
8	37	-0.05117188721949617
8	38	-0.05461021845380998
8	39	-0.054170586650798214
8	40	-0.056123661146585846
8	41	-0.046492994903864826
8	42	-0.06306228592988816
8	43	-0.09441308580164787
8	44	-0.07448657926843083
8	45	-0.06846639170739621
8	46	-0.03312403820615373
8	47	-0.05875456749542707
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112	18	-0.2503786565493584
112	19	-0.25635357548791404
112	20	-0.2557100183085268
112	21	-0.23168018604631158
112	22	-0.26017058335695253
112	23	-0.23870832000432785
112	24	-0.21851239886724727
112	25	-0.2521807891029013
112	26	-0.23480842343767705
112	27	-0.24018301214916704
112	28	-0.23924806575022473
112	29	-0.23941930102400732
112	30	-0.259854938615689
112	31	-0.239493747906927
112	32	-0.22131072417634812
112	33	-0.2364228558914121
112	34	-0.20729534577787234
112	35	-0.21579850614042329
112	36	-0.2774039393310801
112	37	-0.20882471450630646
112	38	-0.17942738307466755
112	39	-0.20267458558850393
112	40	-0.2359842584748364
112	41	-0.18924809324447267
112	42	-0.17263951240514785
112	43	-0.20802058960435232
112	44	-0.16742666245406923
112	45	-0.1685832114386301
112	46	-0.12677239368321835
112	47	-0.1758987665990972
112	48	-0.11046220200896073
112	49	-0.12629976614059046
112	50	-0.15650651050562914
113	1	-0.23593563547581198
113	2	-0.26792759645172737
113	3	-0.2835082103520321
113	4	-0.2777860850944691
113	5	-0.28560841183472974
113	6	-0.27333498286310487
113	7	-0.28745475085735706
113	8	-0.3079158467953249
113	9	-0.28202837321381224
113	10	-0.29142501587929814
113	11	-0.27492514551951786
113	12	-0.34732901823966245
113	13	-0.293908606077224
113	14	-0.285355493064813
113	15	-0.29283709156204907
113	16	-0.2925347539459082
113	17	-0.2686256481543623
113	18	-0.28397393699660967
113	19	-0.2770724732569434
113	20	-0.312839422299902
113	21	-0.2861311847816168
113	22	-0.30675288698655123
113	23	-0.24506078625262262
113	24	-0.2850966891683768
113	25	-0.26907553081012026
113	26	-0.27152639272298984
113	27	-0.24795885653996663
113	28	-0.2904206965793108
113	29	-0.2523784042134807
113	30	-0.25293815373932205
113	31	-0.21879133264207695
113	32	-0.23011658280052671
113	33	-0.2610922435053382
113	34	-0.21809855014139146
113	35	-0.23810287870121982
113	36	-0.22649860058721316
113	37	-0.210268268526157
113	38	-0.21015337958557723
113	39	-0.22844788000686123
113	40	-0.21357021541206933
113	41	-0.19048122375210852
113	42	-0.20728447913111125
113	43	-0.2149292227801638
113	44	-0.1856667176354847
113	45	-0.18018400027953163
113	46	-0.13121060546351515
113	47	-0.14554277025269613
113	48	-0.12255901262247448
113	49	-0.1237878689930767
113	50	-0.1162890329101316
114	1	-0.2583889619631705
114	2	-0.29566669345311425
114	3	-0.28838944190100335
114	4	-0.2938207387457723
114	5	-0.27774225144745657
114	6	-0.2965565922302815
114	7	-0.3197700444721292
114	8	-0.29371217932692417
114	9	-0.30828601991452587
114	10	-0.30823603007358713
114	11	-0.2886264656988402
114	12	-0.2924335514866172
114	13	-0.2941440692874977
114	14	-0.30707715619473674
114	15	-0.28952199578964155
114	16	-0.2854608142309445
114	17	-0.288934463255174
114	18	-0.27820075934811683
114	19	-0.26420070359008463
114	20	-0.3381579551179783
114	21	-0.340168697536555
114	22	-0.2832984644730132
114	23	-0.3308391937854722
114	24	-0.3241263289524383
114	25	-0.33435678504498156
114	26	-0.2802327701299703
114	27	-0.3235896703734582
114	28	-0.3031389109764433
114	29	-0.30151285174415676
114	30	-0.2862910748467371
114	31	-0.2380562355733602
114	32	-0.2441781976183819
114	33	-0.2382567216139293
114	34	-0.25422097588860026
114	35	-0.24773659649486746
114	36	-0.2650303847727997
114	37	-0.24831381043131984
114	38	-0.2352957510824183
114	39	-0.22898635705967507
114	40	-0.20793373074371857
114	41	-0.16505840337147504
114	42	-0.21803603497524904
114	43	-0.1691659081328254
114	44	-0.1671759948148548
114	45	-0.17322554129263068
114	46	-0.167166747247947
114	47	-0.18645189658237626
114	48	-0.1738970788670855
114	49	-0.12736052246429352
114	50	-0.10525688393821753
115	1	-0.2925538085260513
115	2	-0.31582743701232374
115	3	-0.33755344540837484
115	4	-0.3500829893746129
115	5	-0.31288318678069515
115	6	-0.35513917928393823
115	7	-0.35276495268278296
115	8	-0.35733368514109287
115	9	-0.35541954371389295
115	10	-0.33615855320563526
115	11	-0.3306630711845955
115	12	-0.3435945465549324
115	13	-0.3380311931569153
115	14	-0.3451673959213508
115	15	-0.32746746163973484
115	16	-0.2932739447586883
115	17	-0.32658841170815833
115	18	-0.3503622917422149
115	19	-0.3547310945923172
115	20	-0.4221916052930226
115	21	-0.33913253210337047
115	22	-0.32769516364071904
115	23	-0.37308581907679184
115	24	-0.3315734314386418
115	25	-0.34487230249747824
115	26	-0.3440598006625919
115	27	-0.31960998102405824
115	28	-0.35565791266213154
115	29	-0.33412312524155396
115	30	-0.4300744210583076
115	31	-0.318238618703532
115	32	-0.39259119305356194
115	33	-0.3405391601894152
115	34	-0.33824502048060623
115	35	-0.3824912428992846
115	36	-0.33168840588300696
115	37	-0.3083031540524175
115	38	-0.35094080074563294
115	39	-0.3095341528225646
115	40	-0.31365953617477516
115	41	-0.25422439354950654
115	42	-0.27543895383646344
115	43	-0.2479226778303456
115	44	-0.26522655823620056
115	45	-0.20886135851373666
115	46	-0.18315668301490717
115	47	-0.20284163147486112
115	48	-0.23661246025411486
115	49	-0.16579433662589338
115	50	-0.1792681371192799
116	1	-0.293621639344515
116	2	-0.406276296848489
116	3	-0.3646783866097536
116	4	-0.39264669353317316
116	5	-0.3773793959155469
116	6	-0.36022768330477106
116	7	-0.38789936832403527
116	8	-0.36975180779197814
116	9	-0.38316191923854603
116	10	-0.37578385186041885
116	11	-0.3531404994308218
116	12	-0.3869239303383978
116	13	-0.34027215860586324
116	14	-0.3689993810085497
116	15	-0.3509105717569341
116	16	-0.3857194955319301
116	17	-0.3482825977501989
116	18	-0.36788543270488816
116	19	-0.35007111300584626
116	20	-0.3697054488298903
116	21	-0.36271757196003307
116	22	-0.3697275631112902
116	23	-0.3322343572350164
116	24	-0.34479988604979184
116	25	-0.36748401747798454
116	26	-0.296139693152341
116	27	-0.3557822329382745
116	28	-0.3266492981711693
116	29	-0.3015470180905311
116	30	-0.3728661406768268
116	31	-0.26737654750894535
116	32	-0.29465424055823064
116	33	-0.3014874119087665
116	34	-0.24944229913278804
116	35	-0.26770508967176454
116	36	-0.286050106234768
116	37	-0.2814042631453759
116	38	-0.2554415010876454
116	39	-0.2157278879727187
116	40	-0.23542543272120042
116	41	-0.196957298193297
116	42	-0.18824402512338878
116	43	-0.19082795507317485
116	44	-0.21855451130609538
116	45	-0.15412527865294123
116	46	-0.1698955990572628
116	47	-0.18224320671872363
116	48	-0.14457593525234103
116	49	-0.08921406883017369
116	50	-0.10984546450624322
117	1	-0.2939893461861658
117	2	-0.3487946410408256
117	3	-0.3392172246387659
117	4	-0.3444693556988696
117	5	-0.35393090842624275
117	6	-0.3406064472172332
117	7	-0.3515207914394054
117	8	-0.36613463711787375
117	9	-0.3255438090842233
117	10	-0.3606892770964052
117	11	-0.3120626041486787
117	12	-0.3268447763264106
117	13	-0.352623022451958
117	14	-0.36838876408621957
117	15	-0.32669986446796884
117	16	-0.3072589154453951
117	17	-0.3279894813691513
117	18	-0.3547822883040297
117	19	-0.37573522911213786
117	20	-0.36389880449441847
117	21	-0.35843638918446175
117	22	-0.35610403276746894
117	23	-0.3576130388479939
117	24	-0.3507450648286792
117	25	-0.3795460825003616
117	26	-0.3661307252221917
117	27	-0.36074479210120103
117	28	-0.3787094531220987
117	29	-0.3297483457710655
117	30	-0.4819754721898093
117	31	-0.3263627282505368
117	32	-0.36425176354284616
117	33	-0.3860785254822332
117	34	-0.36869136069261543
117	35	-0.3659063395747708
117	36	-0.31875429169691216
117	37	-0.36608581570788346
117	38	-0.34942974112502156
117	39	-0.3220353743856137
117	40	-0.3928537566570185
117	41	-0.31311370808377603
117	42	-0.31912114404107683
117	43	-0.2762141087554255
117	44	-0.30882773472711733
117	45	-0.2792806030946764
117	46	-0.24712166885554154
117	47	-0.3060778777351416
117	48	-0.2454323020396032
117	49	-0.24177820409144601
117	50	-0.22710263446407453
118	1	-0.365015677322134
118	2	-0.3871743587169405
118	3	-0.38786364063532375
118	4	-0.40883279664159744
118	5	-0.3658571705213731
118	6	-0.4047406162829361
118	7	-0.4016455513838011
118	8	-0.3933351343519149
118	9	-0.3816014715120062
118	10	-0.40201613288142823
118	11	-0.360334801097423
118	12	-0.3917191264031601
118	13	-0.41570085850642613
118	14	-0.4436636967169818
118	15	-0.3962642564115768
118	16	-0.3770434031066756
118	17	-0.40286935529607604
118	18	-0.424138973398712
118	19	-0.35285552612987914
118	20	-0.45888863850610306
118	21	-0.3613177729227104
118	22	-0.3401212201467345
118	23	-0.43360940547142945
118	24	-0.43277385336313756
118	25	-0.4275811354615229
118	26	-0.3711390757541366
118	27	-0.35659684145237236
118	28	-0.3994489575514635
118	29	-0.4255243809916962
118	30	-0.39598838416933546
118	31	-0.3341773026272463
118	32	-0.362333955963166
118	33	-0.3643905569475905
118	34	-0.38608458920571564
118	35	-0.34724300157976273
118	36	-0.3779358836436941
118	37	-0.36194334141559636
118	38	-0.3362836236494644
118	39	-0.359410608970542
118	40	-0.3525346833930314
118	41	-0.3138406743555322
118	42	-0.2841768120117436
118	43	-0.340017312021196
118	44	-0.3301129531825211
118	45	-0.3231845372810085
118	46	-0.28955239181063064
118	47	-0.2719010687864625
118	48	-0.26765037248601686
118	49	-0.3009389798858777
118	50	-0.24900692435018357
119	1	-0.3710681536570206
119	2	-0.40788058808976047
119	3	-0.40372929264499646
119	4	-0.4404035865535505
119	5	-0.4281942852910916
119	6	-0.40141017316653205
119	7	-0.4628929433073452
119	8	-0.4232720543077946
119	9	-0.4155598444831057
119	10	-0.435535293729167
119	11	-0.45887454019659657
119	12	-0.42487731067494394
119	13	-0.4455949942778048
119	14	-0.4164221787970419
119	15	-0.41706597993180594
119	16	-0.4334924470683319
119	17	-0.4510260653842053
119	18	-0.47651766484618463
119	19	-0.4891830671664579
119	20	-0.45677949075442825
119	21	-0.4751174137596976
119	22	-0.45499924212402476
119	23	-0.4738662713782418
119	24	-0.4868140999580177
119	25	-0.5075897510308351
119	26	-0.45251057625849
119	27	-0.5411935143593851
119	28	-0.4914684316969584
119	29	-0.5095944360341278
119	30	-0.46409209682317965
119	31	-0.46061387928421027
119	32	-0.5117853245538697
119	33	-0.48039316184139125
119	34	-0.47068959100435137
119	35	-0.46402463721661036
119	36	-0.4144572273690059
119	37	-0.46581618474935027
119	38	-0.4547727558824519
119	39	-0.39729269627871844
119	40	-0.49147096361002696
119	41	-0.37143375594987305
119	42	-0.36678531880227183
119	43	-0.3651214318970446
119	44	-0.3612421523082432
119	45	-0.3743011619550529
119	46	-0.35874750912132924
119	47	-0.32845406198248384
119	48	-0.31291741751432056
119	49	-0.3419695628394024
119	50	-0.3626982427688077
120	1	-0.46729327441808266
120	2	-0.5352843033455414
120	3	-0.5132488396899859
120	4	-0.4985703273593529
120	5	-0.48406988616127933
120	6	-0.4770671601881773
120	7	-0.4920434551282682
120	8	-0.501658780862229
120	9	-0.48255087995304535
120	10	-0.5177375145363214
120	11	-0.4914728959912651
120	12	-0.5263614009511244
120	13	-0.5110345713188593
120	14	-0.49710917213433703
120	15	-0.5099705511575863
120	16	-0.4694904299993752
120	17	-0.5184480473794792
120	18	-0.5013164036547337
120	19	-0.488884768192122
120	20	-0.5259449909798875
120	21	-0.5182141705779344
120	22	-0.5098231015818229
120	23	-0.5380721291338517
120	24	-0.538606159597812
120	25	-0.5565475138391562
120	26	-0.4881921680303378
120	27	-0.5078185829776842
120	28	-0.5409038592449491
120	29	-0.5636075388758215
120	30	-0.5127002207825626
120	31	-0.48295653489144286
120	32	-0.5039164898191615
120	33	-0.4852040415154093
120	34	-0.47367535125908233
120	35	-0.4975079508799354
120	36	-0.4753657153276194
120	37	-0.4826851686319955
120	38	-0.5245490280798606
120	39	-0.4626052894767696
120	40	-0.5172949298505358
120	41	-0.4380699796036964
120	42	-0.460696022051863
120	43	-0.4322890253494691
120	44	-0.40712806867445295
120	45	-0.42586179382049494
120	46	-0.43129815807635197
120	47	-0.4593302747257084
120	48	-0.46414591258433546
120	49	-0.43405867037505885
120	50	-0.3951087442245367
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	42098.0
3	1152.0
4	1966.0
5	2051.0
6	3093.0
7	5119.0
8	5921.0
9	6773.0
10	9042.0
11	11111.0
12	13122.0
13	16322.0
14	19495.0
15	22078.0
16	24064.0
17	26835.0
18	30755.0
19	38971.0
20	50910.0
21	42962.0
22	53873.0
23	70887.0
24	93107.0
25	100663.0
26	129623.0
27	164517.0
28	199840.0
29	227531.0
30	269953.0
31	326730.0
32	396813.0
33	496812.0
34	668113.0
35	969321.0
36	1518617.0
37	2709440.0
38	4793750.0
39	2.2943397E7
40	430403.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	16.282768041475812	28.64936722080462	27.958039533503403	27.109825204216165
2	24.27451425346788	29.80815908466443	25.058987582942216	20.85833907892547
3	24.166990498499228	27.217785532835332	25.772732514076026	22.842491454589418
4	25.544939398052986	25.445794776264268	24.959994251669553	24.049271574013193
5	25.28940873813142	27.987672779785562	26.496655031487432	20.226263450595592
6	25.965096408724552	26.16223992591835	23.288627741908602	24.584035923448493
7	24.278477628873464	26.097462383595833	27.10937605542475	22.514683932105957
8	25.251371042170735	25.911753534306715	25.74012453018269	23.096750893339863
9	23.924714982688197	25.522417355064253	26.365306765017298	24.18756089723025
10	23.309362938152102	27.13338818314205	25.643834147823213	23.913414730882636
11	24.08799739449872	26.735897629573195	25.56470531222834	23.611399663699743
12	23.809126997341163	25.819694654959235	26.710018590998835	23.66115975670076
13	23.437032185540087	26.456558404916603	26.089753289197166	24.01665612034615
14	23.385777249385058	27.306648508265642	26.102509252670746	23.205064989678554
15	23.022696875888162	26.838245307150626	26.5045027562221	23.634555060739114
16	23.457071849399384	25.951114511355065	26.322577235714473	24.26923640353108
17	23.93198677865124	25.92279388573534	26.29406157418951	23.85115776142391
18	23.052726302973703	25.940954027968377	26.251332023669676	24.754987645388248
19	23.59341780637043	26.624633195288332	26.252967534381977	23.528981463959262
20	24.121887320733038	26.110157691846407	26.32989263136407	23.438062356056477
21	23.524552328368966	26.588580139875134	26.23982361427752	23.64704391747838
22	24.04137194274268	26.113759306020423	26.223981789587764	23.620886961649138
23	23.31475627729301	25.962936367714875	27.265003435768538	23.45730391922358
24	23.217704289720814	26.62254740901608	26.098813066277216	24.06093523498589
25	23.47290374897681	25.948258922918	26.136464957852585	24.4423723702526
26	23.659512362800147	26.229007699755712	26.695470605260578	23.41600933218356
27	23.493235888832807	26.065007604429635	26.833486888658424	23.60826961807913
28	23.753554429979477	26.19334967054706	26.389478564983214	23.663617334490247
29	24.11498325684644	25.994063606262465	26.441452721855118	23.44950041503598
30	23.771258944410285	26.154453364964674	26.44539903512711	23.628888655497935
31	23.80184161896804	26.066512859882774	26.47157704492192	23.660068476227263
32	23.63113945629426	26.013452843047364	26.45485069557159	23.900557005086785
33	23.757470497497632	26.051766313921433	26.371696271852326	23.81906691672861
34	23.786807062937985	26.01785298865402	26.44958985607795	23.745750092330045
35	23.554327348589958	26.10637794656676	26.65840679948882	23.68088790535446
36	23.50032936631592	26.01601363484044	26.676759113578036	23.80689788526561
37	23.608841592773345	26.05774280465431	26.302203718622057	24.03121188395029
38	23.572617706443197	26.47559254363323	26.371370294705905	23.580419455217662
39	23.76752117295026	26.054625963466314	26.36618551933424	23.81166734424918
40	23.492308947503297	26.012666818124714	26.379301609165772	24.11572262520622
41	23.551355577214213	26.471978037750425	26.342112904416993	23.63455348061837
42	23.876431900775554	25.98827869029311	26.320201082516103	23.81508832641523
43	23.81350005179803	26.020367632225234	26.31015163538072	23.855980680596016
44	23.595227072118334	26.39501031330529	26.30861510974813	23.701147504828246
45	23.469638315894645	26.279954307370534	26.339688964022862	23.910718412711958
46	23.504386397079482	26.086349549661065	26.539829093515937	23.86943495974352
47	23.92139310828519	26.10012517954525	26.271582071857186	23.706899640312372
48	23.465692329953843	25.99892996388038	26.205803975582377	24.329573730583405
49	23.501146594075287	26.094599577201922	26.16936304975018	24.23489077897261
50	23.90969896786663	26.07761595360631	26.235723923705095	23.77696115482196
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	21302.0
1	11975.5
2	2649.0
3	2098.5
4	1548.0
5	1562.5
6	1577.0
7	1962.0
8	2347.0
9	3441.0
10	4535.0
11	7126.0
12	9717.0
13	14767.5
14	19818.0
15	29451.5
16	39085.0
17	59193.5
18	79302.0
19	112390.0
20	145478.0
21	196343.0
22	247208.0
23	367415.0
24	487622.0
25	487921.0
26	488220.0
27	571761.0
28	655302.0
29	739578.0
30	823854.0
31	938089.0
32	1052324.0
33	1172594.5
34	1292865.0
35	1429358.5
36	1565852.0
37	1687837.0
38	1809822.0
39	1919342.0
40	2028862.0
41	2116137.0
42	2203412.0
43	2284112.0
44	2364812.0
45	2402560.0
46	2440308.0
47	2420863.5
48	2401419.0
49	2392118.5
50	2382818.0
51	2353925.5
52	2325033.0
53	2266959.0
54	2208885.0
55	2107643.5
56	2006402.0
57	1926160.0
58	1845918.0
59	1783892.0
60	1721866.0
61	1499441.0
62	1277016.0
63	1118333.5
64	959651.0
65	833968.0
66	708285.0
67	610092.5
68	511900.0
69	436875.0
70	361850.0
71	281312.0
72	200774.0
73	164210.5
74	127647.0
75	93875.5
76	60104.0
77	44971.5
78	29839.0
79	20501.5
80	11164.0
81	7866.5
82	4569.0
83	3512.5
84	2456.0
85	1817.5
86	1179.0
87	778.5
88	378.0
89	236.0
90	94.0
91	65.5
92	37.0
93	27.0
94	17.0
95	19.0
96	21.0
97	22.5
98	24.0
99	43.5
100	63.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.14422846542634626
2	5.928977348869961E-4
3	0.005162812696025121
4	0.0034869967239016027
5	3.0050981083313503E-4
6	0.0018599120724537275
7	2.3553471659894365E-4
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	4.791913199771612E-4
14	1.5431584880620447E-4
15	2.057544650749393E-4
16	2.9780251524004372E-5
17	0.0
18	2.9780251524004372E-5
19	0.0
20	0.0
21	0.0
22	3.2487547117095677E-4
23	0.0021847875436246844
24	0.0010125285518161485
25	0.013084359601410285
26	0.05367213513303515
27	0.08066116490056238
28	0.033930535668213344
29	0.08662262979654944
30	0.0867904821233211
31	0.17009126022714752
32	0.17120937330709424
33	0.1406954446773621
34	0.17347267242291856
35	0.14966200768168053
36	0.2003913125050254
37	0.21273116581833562
38	0.1995276852108293
39	0.19852869313697863
40	0.18662471441415612
41	0.2180239287028291
42	0.2454569549476233
43	0.24961536097861153
44	0.2506739135555103
45	0.2416911067776333
46	0.263149131648475
47	0.25489188008954655
48	0.25850070511513723
49	0.2880833240608459
50	0.29914804114981014
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	3.693723E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.37467280034389
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.24167373999248	33.503323250230885
2	11.070745015367962	9.603798853952469
3	4.0168904133371	5.226939220600045
4	2.0175001117094133	3.500336288802122
5	1.2109432455008942	2.6262133526693896
6	0.7532130214931981	1.9602221013735517
7	0.5315670579116955	1.613958304585287
8	0.3983530787403551	1.3822747559498023
9	0.3118261395409406	1.2172821095864406
>10	2.031850218190473	17.34013417900278
>50	0.26052575877933265	7.835131229139335
>100	0.14958701038503686	11.618199884627204
>500	0.004691537214024397	1.2917572992986313
>1k	5.399737346469063E-4	0.36068117439623404
>5k	5.620686303344142E-5	0.15848598874837735
>10k+	3.7471239247655264E-5	0.7612620070377083
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	116911	0.31651263508389776	No Hit
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCG	101126	0.2737779741469515	Illumina Paired End PCR Primer 2 (100% over 31bp)
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.842660372745872E-4	0.0	0.0	2.7072955930913067E-6	0.0
2	2.923879240538611E-4	0.0	0.0	2.7072955930913067E-6	0.0
3	3.0050981083313503E-4	0.0	0.0	2.7072955930913067E-6	0.0
4	3.0321710642622633E-4	0.0	0.0	2.7072955930913067E-6	0.0
5	3.1946087998477416E-4	0.0	0.0	2.7072955930913067E-6	0.0
6	3.1946087998477416E-4	0.0	0.0	5.414591186182613E-6	0.0
7	3.302900623571394E-4	0.0	0.0	5.414591186182613E-6	0.0
8	3.329973579502307E-4	0.0	0.0	5.414591186182613E-6	0.0
9	3.4382654032259595E-4	0.0	0.0	5.414591186182613E-6	0.0
10	6.768238982728267E-4	0.0	0.0	5.414591186182613E-6	0.0
11	8.934075457201311E-4	0.0	0.0	5.414591186182613E-6	0.0
12	0.0012643070419736402	5.414591186182613E-6	0.0	5.414591186182613E-6	0.0
13	0.0015864752175515056	5.414591186182613E-6	0.0	5.414591186182613E-6	0.0
14	0.001686645154495884	8.121886779273919E-6	0.0	5.414591186182613E-6	0.0
15	0.015047148906401481	8.121886779273919E-6	0.0	1.0829182372365227E-5	0.0
16	0.015420755698248082	8.121886779273919E-6	0.0	1.0829182372365227E-5	0.0
17	0.01658218550768425	8.121886779273919E-6	0.0	1.0829182372365227E-5	0.0
18	0.017313155317818907	8.121886779273919E-6	0.0	1.3536477965456532E-5	0.0
19	0.019013336950280246	8.121886779273919E-6	0.0	1.3536477965456532E-5	2.7072955930913067E-6
20	0.30433792680176613	8.121886779273919E-6	0.0	1.3536477965456532E-5	2.7072955930913067E-6
21	0.31015049044013315	8.121886779273919E-6	0.0	1.8951069151639146E-5	2.7072955930913067E-6
22	0.3111873846522871	8.121886779273919E-6	0.0	1.8951069151639146E-5	2.7072955930913067E-6
23	0.3121593037702069	8.121886779273919E-6	0.0	1.8951069151639146E-5	2.7072955930913067E-6
24	0.31316371043524377	8.121886779273919E-6	0.0	1.8951069151639146E-5	2.7072955930913067E-6
25	0.3140273377294399	8.121886779273919E-6	0.0	1.8951069151639146E-5	2.7072955930913067E-6
26	0.3152239623815863	8.121886779273919E-6	0.0	1.8951069151639146E-5	2.7072955930913067E-6
27	0.3163935140778017	8.121886779273919E-6	0.0	2.1658364744730453E-5	2.7072955930913067E-6
28	0.3585867158961297	8.121886779273919E-6	0.0	2.7072955930913065E-5	2.7072955930913067E-6
29	0.3609935016783879	8.121886779273919E-6	0.0	2.7072955930913065E-5	2.7072955930913067E-6
30	0.36455900997448915	8.121886779273919E-6	0.0	2.7072955930913065E-5	2.7072955930913067E-6
31	0.42341561616829415	1.0829182372365227E-5	0.0	2.7072955930913065E-5	2.7072955930913067E-6
32	0.4256112328942912	1.0829182372365227E-5	0.0	2.7072955930913065E-5	2.7072955930913067E-6
33	0.42912800986971683	1.0829182372365227E-5	2.7072955930913067E-6	2.7072955930913065E-5	2.7072955930913067E-6
34	0.431150359677756	1.3536477965456532E-5	2.7072955930913067E-6	2.9780251524004372E-5	2.7072955930913067E-6
35	0.43341636608917344	1.3536477965456532E-5	2.7072955930913067E-6	3.2487547117095677E-5	2.7072955930913067E-6
36	0.43608846683955454	1.3536477965456532E-5	2.7072955930913067E-6	3.2487547117095677E-5	5.414591186182613E-6
37	0.43914229626856155	1.3536477965456532E-5	2.7072955930913067E-6	3.2487547117095677E-5	5.414591186182613E-6
38	0.4426211711056839	1.8951069151639146E-5	2.7072955930913067E-6	3.519484271018699E-5	5.414591186182613E-6
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCAACGC	42180	0.0	30.979387	12
ATCAACG	43150	0.0	30.38991	11
CAACGCA	43570	0.0	29.864983	13
AAGCAGT	46545	0.0	29.273422	1
GTATCAA	45335	0.0	29.031843	9
AACGCAG	44795	0.0	28.964767	14
ACGCAGA	44555	0.0	28.903763	15
GGTATCA	46125	0.0	28.610786	8
GTGGTAT	46700	0.0	28.287203	6
CGCAGAG	46290	0.0	27.754	16
TGGTATC	47560	0.0	27.738255	7
AGTGGTA	48920	0.0	27.11585	5
TATCAAC	48735	0.0	26.983892	10
AGAGTAC	46745	0.0	26.633045	19
AGCAGTG	53115	0.0	25.634373	2
CAGAGTA	49235	0.0	25.519228	18
GCAGAGT	50330	0.0	25.273619	17
GTACTTT	28185	0.0	23.515692	22
CAGTGGT	57250	0.0	23.436085	4
ATGCCGA	20800	0.0	23.356354	14
>>END_MODULE