FastQCFastQC Report
Thu 26 May 2016
SRR522105_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR522105_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33724144
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAG2104130.6239239163490703Illumina Paired End PCR Primer 2 (97% over 36bp)
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1029280.3052056710468322No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGA326050.037.25263225
AATGCCG340500.035.767224
AGATCGG297300.035.62655631
TGCCGAG342150.035.5210926
CGAGATC325750.033.43316729
CCGAGAT338100.032.23677428
GAGATCG337450.031.92896530
GCCGAGA395000.030.88822227
GTTCAGC429350.028.90428514
TATGCCG38500.028.8690543
TCGTATG39150.028.219540
GACCGAT40650.027.66401132
GGAATGC447350.027.56156522
GAATGCC445000.027.52098723
CAGGAAT470450.026.36880720
CTCGTAT43700.025.78366539
AGGAATG480900.025.70025821
AAGCAGT337000.024.9610481
CGTATGC44950.024.92192541
GCAGGAA502350.024.89817819
Produced by FastQC (version 0.11.5)