##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR522105_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	33724144
Sequences flagged as poor quality	0
Sequence length	50
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.2351267388729	39.0	38.0	40.0	35.0	40.0
2	38.01030582125376	39.0	38.0	40.0	35.0	40.0
3	37.99662161921738	39.0	38.0	40.0	35.0	40.0
4	37.96524813202078	39.0	38.0	40.0	34.0	40.0
5	37.95075252317746	39.0	38.0	40.0	35.0	40.0
6	37.94362706433705	39.0	38.0	40.0	35.0	40.0
7	37.88424281428759	39.0	38.0	40.0	33.0	40.0
8	37.84235881569003	39.0	38.0	40.0	33.0	40.0
9	37.68742130267265	39.0	38.0	40.0	33.0	40.0
10	37.6083832698615	39.0	38.0	40.0	33.0	40.0
11	37.705449810675695	39.0	38.0	40.0	33.0	40.0
12	37.65887937733868	39.0	38.0	40.0	33.0	40.0
13	37.7169799773124	39.0	38.0	40.0	33.0	40.0
14	37.68788746721044	39.0	38.0	40.0	33.0	40.0
15	37.689442614169835	39.0	38.0	40.0	33.0	40.0
16	37.73723807489376	39.0	38.0	40.0	33.0	40.0
17	37.59678377603891	39.0	38.0	40.0	33.0	40.0
18	37.53726377754762	39.0	38.0	40.0	33.0	40.0
19	37.47013638062985	39.0	37.0	40.0	33.0	40.0
20	37.379359487968024	39.0	37.0	40.0	33.0	40.0
21	37.55115355930161	39.0	38.0	40.0	33.0	40.0
22	37.498735060554836	39.0	37.0	40.0	33.0	40.0
23	37.378360411460704	39.0	37.0	40.0	33.0	40.0
24	37.40070600457643	39.0	37.0	40.0	33.0	40.0
25	37.34884989223151	39.0	37.0	40.0	33.0	40.0
26	37.304276040334784	39.0	37.0	40.0	33.0	40.0
27	37.205724183836956	39.0	37.0	40.0	33.0	40.0
28	37.170208263848004	39.0	36.0	40.0	33.0	40.0
29	37.09503488064812	39.0	36.0	40.0	33.0	40.0
30	37.03271246855072	39.0	36.0	40.0	32.0	40.0
31	36.99921477621493	39.0	36.0	40.0	33.0	40.0
32	36.87771582875462	39.0	36.0	40.0	32.0	40.0
33	36.776803853049614	39.0	36.0	40.0	31.0	40.0
34	36.729819295042745	39.0	36.0	40.0	31.0	40.0
35	36.87343139087533	39.0	36.0	40.0	32.0	40.0
36	36.7226714486808	39.0	36.0	40.0	31.0	40.0
37	36.63278611311824	39.0	36.0	40.0	31.0	40.0
38	36.555490630095754	39.0	36.0	40.0	31.0	40.0
39	36.55337105072259	39.0	36.0	40.0	31.0	40.0
40	36.275550566976584	39.0	35.0	40.0	30.0	40.0
41	36.432456521357516	39.0	36.0	40.0	31.0	40.0
42	36.48178373333953	39.0	36.0	40.0	31.0	40.0
43	36.50506740808603	39.0	36.0	40.0	31.0	40.0
44	36.41762346881214	39.0	36.0	40.0	31.0	40.0
45	36.391742634001325	39.0	36.0	40.0	31.0	40.0
46	36.395377033142786	39.0	36.0	40.0	31.0	40.0
47	36.17606513600464	39.0	35.0	40.0	30.0	40.0
48	36.10053352280787	39.0	35.0	40.0	30.0	40.0
49	35.885274775247076	38.0	35.0	40.0	30.0	40.0
50	35.83609970945445	38.0	35.0	40.0	30.0	40.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1	1	-0.07287461300590792
1	2	-0.12059537602859649
1	3	-0.05929535328163382
1	4	-0.06608186975130792
1	5	-0.10673697104013513
1	6	0.0354602388167109
1	7	0.018642234799983726
1	8	0.0521324909439187
1	9	0.06363469011242984
1	10	0.23890387975975358
1	11	-0.3246536977938703
1	12	-0.04214631843193928
1	13	-0.1445578546055586
1	14	0.011122992967386836
1	15	-0.14745458397747768
1	16	-0.10395712203609264
1	17	0.06265851230246255
1	18	-0.003702165129176649
1	19	-0.2792726073553311
1	20	-0.1113878972254696
1	21	0.04828576800175455
1	22	-0.1095464902247798
1	23	-0.027484821007810467
1	24	-0.2514417640351141
1	25	-0.03619844808562789
1	26	-0.05208902295029105
1	27	-0.042252940172026854
1	28	-0.02151167708616697
1	29	-0.04648676379316896
1	30	-0.0861605544920323
1	31	-0.06779799331419412
1	32	-0.028389214361951076
1	33	-0.05619964897238816
1	34	-0.03467987364069103
1	35	0.06441649998608767
1	36	0.22355842454416575
1	37	-1.170641312084065
1	38	0.2764951265368225
1	39	0.1343704969788817
1	40	0.2884565140482209
1	41	0.37599456523979313
1	42	0.23838788313151582
1	43	0.20377554168391754
1	44	0.2782080522316406
1	45	0.11675056213749002
1	46	0.18105035351762666
1	47	0.2774131047632977
1	48	0.11614766271485877
1	49	0.4459976882454697
1	50	0.38544466510843023
2	1	-0.04877113192057436
2	2	-0.06964226076890867
2	3	-0.0728004142979728
2	4	-0.08051150797422224
2	5	-0.1202710962992839
2	6	0.010051250134736733
2	7	-0.12347770302296368
2	8	0.04771351889573339
2	9	0.13404837426365646
2	10	-0.38915450501119864
2	11	0.019498879885652798
2	12	0.06182427808765567
2	13	-0.003877138467565544
2	14	5.178562416361387E-4
2	15	0.01051706615960768
2	16	-0.14265178158207448
2	17	0.09795021020519812
2	18	0.08724371966499689
2	19	0.1851196404465938
2	20	0.1649844519391621
2	21	0.09627005907256603
2	22	0.04223416667043978
2	23	0.1379010586606526
2	24	0.10502953355487676
2	25	0.07794327151469105
2	26	0.051596409704544044
2	27	-0.047577948977966855
2	28	-0.00484372073886874
2	29	0.032609390874412725
2	30	0.0683948864023165
2	31	-0.056006298816221545
2	32	-0.04448743107410991
2	33	0.0059392688225443635
2	34	-0.13007688995938338
2	35	0.07850937680136383
2	36	0.2625770312309825
2	37	0.15628183388653127
2	38	0.41383023570880795
2	39	0.308148792829968
2	40	0.5460501433147726
2	41	0.5183910312259599
2	42	0.34510098036456327
2	43	0.2466463522079536
2	44	0.25589866135502604
2	45	0.03754601239182165
2	46	0.13357863311873786
2	47	0.30686453033499816
2	48	0.21193266655604504
2	49	0.41675342665366344
2	50	0.1983330627493629
3	1	-0.02893975417084249
3	2	-0.05205226349634273
3	3	-0.07417413281397245
3	4	-0.044788023302658075
3	5	-0.08781271731416496
3	6	0.022244713176938546
3	7	-0.14680056514077933
3	8	0.032077334092861065
3	9	0.13252723134851863
3	10	0.2362883213491287
3	11	-0.04043555699847445
3	12	-0.019789505829827192
3	13	0.01091892367627878
3	14	0.07056282553435267
3	15	0.07502076160767501
3	16	-0.0039484322739724576
3	17	0.14356881030474256
3	18	-0.04005527078502524
3	19	0.12329391902967046
3	20	0.17762380858683713
3	21	0.058218954528960865
3	22	-0.1278509942296111
3	23	0.03221570013796082
3	24	0.08179891135307571
3	25	-0.046908164268053554
3	26	0.010635543984186313
3	27	0.023535337801639855
3	28	0.04489435813358966
3	29	-0.10139230162259594
3	30	0.014176542291004068
3	31	-0.036452050284758286
3	32	-0.00758106477052678
3	33	-0.031032285353660427
3	34	-0.05213362936616761
3	35	0.026729961473130004
3	36	0.1997874731334761
3	37	-0.16199411608347702
3	38	0.04803767671977255
3	39	-0.9213751925905953
3	40	0.4410454204270948
3	41	0.3037533804635686
3	42	0.050656640334416636
3	43	0.0884491279950197
3	44	0.0678936473826468
3	45	0.05298624031257759
3	46	0.11150670571660015
3	47	0.21872746470661042
3	48	0.15257267315286072
3	49	0.030850828694646282
3	50	-0.0563031674215182
4	1	-0.20093264194190397
4	2	-0.0927432609897707
4	3	-0.05446615935986898
4	4	-0.07330521441496529
4	5	-0.0383549403481922
4	6	0.05577216361255921
4	7	0.05271990377994484
4	8	-0.03857119289263977
4	9	0.1729313002653683
4	10	0.14926166970803223
4	11	-0.13658712234693127
4	12	0.1173561369490912
4	13	-0.05937550718605422
4	14	0.08227288227175222
4	15	0.021474342300102478
4	16	0.028737172891560192
4	17	-0.15510928311294236
4	18	-0.516842000292904
4	19	-0.20880922860862938
4	20	0.18313294955958526
4	21	0.05890112387186974
4	22	-0.039058890605524255
4	23	-0.10783563331905555
4	24	-0.010294578790414732
4	25	-0.03523707128942988
4	26	-0.5502769231448497
4	27	-0.08889449419797302
4	28	-0.1267280573315901
4	29	-0.4540788968314473
4	30	-0.13792548293037754
4	31	-0.049175703454835684
4	32	-0.08522999606244497
4	33	-0.08444093315459611
4	34	-0.5045764084336071
4	35	-0.0921043481723487
4	36	0.006367183319831327
4	37	0.038330153187310145
4	38	-0.3284213447144495
4	39	0.07979928099344136
4	40	0.3031384057088857
4	41	-0.09667732704382814
4	42	0.11541605502988261
4	43	-0.008162596357806251
4	44	-0.06920500428861942
4	45	-0.11735396718285074
4	46	0.01613411252236574
4	47	-0.05071293660600418
4	48	0.10530251112861322
4	49	-0.07125751500932864
4	50	-0.2521705420287432
5	1	-0.06828895374692223
5	2	-0.0499110131519771
5	3	-0.07127393298573992
5	4	-0.04442757536368447
5	5	-0.05382923438095588
5	6	-0.027534105677105458
5	7	-0.00239749336616768
5	8	-0.04814523085254763
5	9	-0.042090614468094145
5	10	-1.6921150430414116
5	11	-0.11077048638884435
5	12	0.09753634005625855
5	13	-0.03746413338702581
5	14	-0.03158847809895349
5	15	-0.10293625626653835
5	16	0.04262494488718005
5	17	-0.0025341928213222786
5	18	-0.37131747835016427
5	19	0.14348215460380231
5	20	0.20883493317420943
5	21	0.12414330443502308
5	22	0.054324999114086836
5	23	-0.0913368169847999
5	24	0.07717740001485396
5	25	-0.05444654448720598
5	26	0.07325533879232182
5	27	0.061698854871380604
5	28	0.04150798272905121
5	29	-0.033190233116648926
5	30	-0.1465552399329937
5	31	0.029466410738997695
5	32	0.06050205270744158
5	33	0.09103570606425393
5	34	0.02319749809146998
5	35	0.06149899493843236
5	36	0.2696249897295786
5	37	0.43986427278582596
5	38	0.0380780517758339
5	39	0.3682074044859647
5	40	0.3689827193349444
5	41	0.4641569585765879
5	42	0.3670720395261071
5	43	0.26481328230863
5	44	0.2860756295262874
5	45	0.2686339595326359
5	46	0.2925149293034295
5	47	0.21242923476049214
5	48	0.323548658361112
5	49	0.38948633229850316
5	50	0.3913492261296412
6	1	0.007612656987198818
6	2	0.0192954598980819
6	3	-0.031340935692540484
6	4	-0.03179669822593212
6	5	0.03709180759440045
6	6	0.03746616237935285
6	7	0.09093463086145448
6	8	0.0925627347586584
6	9	-0.7810686463862027
6	10	-0.228827085392048
6	11	0.10576694284710442
6	12	0.15161501861778248
6	13	-1.118761948669892
6	14	-0.16315714446676566
6	15	-0.5697132253122916
6	16	0.04805651007451672
6	17	0.041764417505582685
6	18	0.10592994688204982
6	19	0.1561771941906116
6	20	-0.6697891045316382
6	21	-0.05700582506590024
6	22	-0.10029395578512634
6	23	0.12449767697594183
6	24	-0.16017746452538262
6	25	-0.8960209027771597
6	26	-0.010287877041029958
6	27	-0.3556254056064887
6	28	-0.11553545724512304
6	29	-0.20161409538810204
6	30	-0.0806116412922151
6	31	0.009296018087624702
6	32	0.023822519330991554
6	33	-0.07245905428757737
6	34	-0.04789734712588967
6	35	-0.00548945353940411
6	36	0.06861544349781923
6	37	-1.9069226428653678
6	38	0.15758531838706347
6	39	0.08949485111705968
6	40	-0.049992654831939376
6	41	-0.40289531015057634
6	42	-0.04004668438829384
6	43	-0.09647491716187773
6	44	-0.5836925643777633
6	45	-0.17850274127415844
6	46	-1.495376276277618
6	47	-0.1975565525441283
6	48	-0.26835934466393496
6	49	-0.06891957589387232
6	50	-0.7798575754321604
7	1	-0.09118124533475225
7	2	-0.084172753198402
7	3	-0.08776156689261683
7	4	-0.11276952321608036
7	5	-0.21263680235903593
7	6	-0.27820605405482723
7	7	-0.08656207884210687
7	8	0.0407612833500508
7	9	-0.14450671298870077
7	10	0.19841514630681445
7	11	0.12360865969016288
7	12	-0.030731315595346587
7	13	0.08373450325080256
7	14	0.0123501432412354
7	15	-0.037819851447139285
7	16	-0.21658021727434118
7	17	-0.5268817764810905
7	18	0.011271390495515732
7	19	-0.4488578840762685
7	20	0.09657532739175423
7	21	-0.39507026018622327
7	22	-0.24179707978825604
7	23	-0.28390331673588065
7	24	-0.5775887860532691
7	25	-0.07633416916419833
7	26	-0.21290963473322932
7	27	-0.1544779025769003
7	28	-0.21015545705735406
7	29	-0.3537754533246513
7	30	-0.06645657625587376
7	31	-0.9476506355745897
7	32	-0.1698843549200717
7	33	-0.1574478133900783
7	34	-0.365536588101385
7	35	-0.4654021599425917
7	36	0.156934848441729
7	37	0.004286226982998187
7	38	-0.20531858339352738
7	39	-0.17268893013643094
7	40	0.29731110437653996
7	41	-0.3124455731632736
7	42	-0.1930804817472236
7	43	-0.4160514923495313
7	44	-0.9959856956503828
7	45	-0.18821388735820221
7	46	-0.49294404397279123
7	47	-0.008695126281217824
7	48	-0.09030850701883963
7	49	-0.03574543814166731
7	50	-0.15263078639591043
8	1	-0.006505588776903437
8	2	0.013386483423765583
8	3	-0.03816787199879457
8	4	-0.10100279030218928
8	5	0.019156743486718142
8	6	-0.06858790273489745
8	7	0.052009326015522106
8	8	-0.03425406046009982
8	9	0.010574046208994048
8	10	0.28535349724359804
8	11	0.16565455683915076
8	12	0.22209481655862362
8	13	0.1342286331933309
8	14	0.001824331178625016
8	15	0.001432692491945886
8	16	-0.8054700380568178
8	17	-0.2888627904898158
8	18	-0.06298519603427621
8	19	-0.029425161116364507
8	20	-0.8487039017936624
8	21	-0.09651796627915132
8	22	-0.07826721230182443
8	23	0.05392751132326623
8	24	-0.013602962233065341
8	25	-0.08996714397884631
8	26	-0.2092531183569477
8	27	0.003594107618965836
8	28	0.039560815236427516
8	29	-0.2168950063628472
8	30	-0.16723986264749158
8	31	-0.0492285689450398
8	32	0.017364427559797946
8	33	0.07516465677083772
8	34	-0.03857646452926389
8	35	-0.045149862491840054
8	36	-0.1015193962350196
8	37	0.026996152248173644
8	38	0.16616227506850834
8	39	0.0261487655671786
8	40	-0.08282033724982085
8	41	-0.3296188409643932
8	42	-0.19259938424024625
8	43	-0.7693735702298099
8	44	-0.1954447239118693
8	45	-1.5068092180985673
8	46	-0.03925424286060064
8	47	0.028973820584496934
8	48	-0.050025277661077894
8	49	-0.39475456809265097
8	50	-0.12514245425840187
9	1	-0.015489405946276236
9	2	0.020415418971985844
9	3	-0.012803861843657671
9	4	-0.00909795355018872
9	5	0.01271479528359265
9	6	-0.05834233446626058
9	7	-0.21127602099544163
9	8	-0.1331258537571287
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114	45	0.35447826707429186
114	46	0.014389259701289348
114	47	0.23423797890622922
114	48	0.4935503831434005
114	49	0.39601514765274715
114	50	0.15316788293622352
115	1	-0.23668742088768369
115	2	-0.24180016139101213
115	3	-0.25078734005683145
115	4	-0.23685501648559892
115	5	-0.20265230571011728
115	6	-0.24590977167284933
115	7	-0.12672499794867775
115	8	-0.24219430522672525
115	9	0.012303445838533378
115	10	0.04208936433744981
115	11	0.024023826670692472
115	12	0.05249736326975807
115	13	-0.06713721275089313
115	14	-0.33352717898167583
115	15	-0.0645978713249491
115	16	-0.18784359581884047
115	17	-0.010710489065161255
115	18	0.01299068736641118
115	19	0.12274153628521134
115	20	0.09230860138698915
115	21	-0.14261966399537584
115	22	-0.03707119379521373
115	23	-0.056103094081521476
115	24	-0.03640256495926053
115	25	-0.4342687360837161
115	26	-0.4270238257067902
115	27	-0.102112082683945
115	28	-0.2875298359697638
115	29	-0.11812536005145091
115	30	-0.631364219197998
115	31	-0.16333285714912904
115	32	-0.20210457824335037
115	33	-0.20760386338424297
115	34	-0.13608835292049548
115	35	-0.07299587237990579
115	36	0.328656184559037
115	37	0.37176261818029843
115	38	0.3770881025170638
115	39	0.1327132850765551
115	40	0.49125724167824814
115	41	-1.4765359885822562
115	42	-0.08300650561943002
115	43	-0.1367657566311209
115	44	0.047543035567848335
115	45	-0.0699045545090442
115	46	0.107524115019622
115	47	0.4481545544466172
115	48	0.363218436684825
115	49	0.47752170136405425
115	50	0.5002464390385626
116	1	-0.42928129509857627
116	2	-0.5366233685789652
116	3	-0.4191200164989297
116	4	-0.3924993599840505
116	5	-0.3366800020259575
116	6	-0.3527306808548545
116	7	-0.275399061878673
116	8	-0.27652304032142183
116	9	-0.24186269197502241
116	10	-0.12999371124957548
116	11	-0.5366305438827652
116	12	-0.07142891349299418
116	13	-0.1959472064046608
116	14	-0.21066685632560933
116	15	-0.22181664282700098
116	16	-0.08063239972010194
116	17	-0.028710128331667306
116	18	-0.0016352654042250947
116	19	-0.05279717910274684
116	20	0.08955823564330956
116	21	0.015690312957765684
116	22	0.006087361934774549
116	23	0.027159005521006918
116	24	-0.006432345513047721
116	25	-0.0017749052944395771
116	26	-0.05745923103094697
116	27	-0.3659490578668141
116	28	-0.15086004739966796
116	29	-0.005143871023761903
116	30	-0.03227137040545358
116	31	0.04644708206060244
116	32	-0.09860661237942736
116	33	-0.035985679744172216
116	34	-0.08126653522451477
116	35	0.06827952091980194
116	36	0.40440310997404794
116	37	0.4517191431143317
116	38	0.5432183540284754
116	39	0.47611449420177365
116	40	0.5642358448380094
116	41	0.7124843794995073
116	42	0.4970561818450818
116	43	0.35954308467954377
116	44	0.4918031377756691
116	45	0.2957459481797571
116	46	0.46765654429248116
116	47	0.4348988545234391
116	48	0.40560416767116436
116	49	0.3923864597060174
116	50	0.41920338482832875
117	1	-0.3362441632107789
117	2	-0.4010085088740709
117	3	-0.3758714894220674
117	4	-0.3300092430269146
117	5	-0.6111067764795024
117	6	-0.24984789311700695
117	7	-0.3137010710403274
117	8	-0.25595860885107413
117	9	-0.05971099385573808
117	10	-0.032179595437021646
117	11	0.010652826079898148
117	12	-0.02378055246457933
117	13	-0.04110957450617292
117	14	-0.14757351067596858
117	15	-0.1710683690385082
117	16	-0.08166163892393286
117	17	-0.029317553536721164
117	18	-0.05077297800855973
117	19	-0.016064915057576457
117	20	0.020206823871788515
117	21	-0.15219386606735696
117	22	-0.12986591660797586
117	23	-0.030589270772388488
117	24	-0.27518927336954135
117	25	-0.06987813118891495
117	26	-0.31145972956000634
117	27	-0.3213544564878035
117	28	-0.20283849094759177
117	29	-0.20720070045886985
117	30	-0.17182209520456126
117	31	-0.22270666938658223
117	32	-0.19298563591374318
117	33	-0.09119133843808669
117	34	-0.1318284719177214
117	35	-0.35835712989864277
117	36	0.10838079949277102
117	37	0.2668542051045648
117	38	0.2994042741373022
117	39	-0.1530340779580115
117	40	0.4630519635606163
117	41	0.433261109522995
117	42	0.25798848235524474
117	43	0.09253173549543448
117	44	0.020832831486217174
117	45	0.05896784120557186
117	46	-0.935085256825765
117	47	-0.10381923080944944
117	48	0.07802679347180685
117	49	-0.005927859723200868
117	50	0.2990666266987603
118	1	-0.43377050875001544
118	2	-0.3706439567416595
118	3	-0.4804262287795211
118	4	-0.4225721767201591
118	5	-0.30551237148371513
118	6	-0.2579922371656451
118	7	-0.25286093882557026
118	8	-0.20934807943063305
118	9	-0.14078063387954387
118	10	0.01263529264329577
118	11	-0.010676832195414931
118	12	0.010921257154492992
118	13	-0.12890480446264974
118	14	-0.07505071674233221
118	15	-0.12086486081008019
118	16	-0.15216658180987253
118	17	-0.0979942075403315
118	18	-0.0703549627943687
118	19	-0.009500611069185538
118	20	0.07779640309029645
118	21	-0.03867376560474867
118	22	-0.1827773718200234
118	23	-0.04090200330610827
118	24	-0.10794308206710923
118	25	-0.404911776906026
118	26	-0.25181667636801564
118	27	-0.1722667023584492
118	28	-0.23421795437553072
118	29	-0.6211581416816401
118	30	-1.0949585613656225
118	31	-0.27636390422836854
118	32	-0.21911440545969185
118	33	-0.36618762952433315
118	34	-0.34909884545132996
118	35	-0.23479800779480797
118	36	0.33800412236994504
118	37	0.42903887433701726
118	38	0.45258386911452675
118	39	0.3951899722783594
118	40	0.6391527309283802
118	41	0.6071269554344028
118	42	0.40653616458654085
118	43	0.3768707798245643
118	44	0.3445334638939741
118	45	0.39101248234185704
118	46	0.07897100998595619
118	47	0.5094456544026755
118	48	0.38434510876282246
118	49	0.575154519291381
118	50	0.47556117574141155
119	1	-0.3225115426978391
119	2	-0.35820905851602447
119	3	-0.35138863513442686
119	4	-0.32874245085399423
119	5	-0.38772095115820804
119	6	-0.17385516300749515
119	7	-0.17821475638212547
119	8	-0.2373897890857748
119	9	-0.06272571056597798
119	10	-0.02684727611617177
119	11	-0.27542975195128605
119	12	-0.11580239149439109
119	13	-0.1908523653699561
119	14	-0.22378004591813294
119	15	-0.31053112388091364
119	16	-0.37907556676010756
119	17	-0.13132412037720798
119	18	-0.155071644248423
119	19	-0.19101407153804928
119	20	-0.08397645084112071
119	21	-0.14495035384989308
119	22	-0.36059064767687943
119	23	-0.09388323710496849
119	24	-0.16985855211935075
119	25	-0.22112781970940176
119	26	-0.09045387435799057
119	27	-0.09072991653071227
119	28	-0.20349040605731972
119	29	-0.1377009916855556
119	30	-0.14542263020694435
119	31	-0.26416947410867664
119	32	-0.27353043703079294
119	33	-0.14749422580006666
119	34	-0.12783032362418112
119	35	-0.17692982500636134
119	36	0.157907466041749
119	37	0.25205974266455655
119	38	0.2427525375110875
119	39	0.340094316123043
119	40	0.5118158577983465
119	41	0.3511270557740147
119	42	0.16798706334094504
119	43	0.17717884065677225
119	44	0.15392871632831628
119	45	0.16417976649914579
119	46	-0.4015849560670972
119	47	0.0017847567332012204
119	48	0.22914507816796004
119	49	0.32029348947394
119	50	0.14420527426065632
120	1	-0.3289453676389158
120	2	-0.3456834966559441
120	3	-0.2920613383500026
120	4	-0.2814295162906859
120	5	-0.36496024455937714
120	6	-0.16900260827057423
120	7	-0.1825444336424198
120	8	-0.15425090969608135
120	9	-0.05537698255378842
120	10	0.008012884824637467
120	11	-0.11605029805405565
120	12	-0.11508144153042821
120	13	-0.22098537685907615
120	14	-0.17048833393410945
120	15	-0.3479556052497017
120	16	-0.32956096330274676
120	17	-0.09208148566204244
120	18	-0.06097980455777474
120	19	-0.04593866242308309
120	20	-0.09029177369334462
120	21	-0.1569978756207604
120	22	-0.35295239025933967
120	23	-0.10433367893291035
120	24	-0.36142218503237444
120	25	-0.1796257988675265
120	26	-0.6066163230464596
120	27	-0.5057992319680267
120	28	-0.2655319737169606
120	29	-0.3005941725428869
120	30	-0.46132685761423176
120	31	-0.5366288524290113
120	32	-0.4245454957151438
120	33	-0.7449714990084431
120	34	-0.5812436604126177
120	35	-0.2025912597346462
120	36	0.2085924409201212
120	37	0.2962142770679179
120	38	0.26786616979973843
120	39	0.2423833734732952
120	40	0.529571580072357
120	41	0.555171112657824
120	42	0.22792885729186452
120	43	0.29572422682741717
120	44	0.06412510967782481
120	45	0.1830125042711046
120	46	0.23945245044259167
120	47	-0.1613343806834493
120	48	0.32124611782439416
120	49	0.3315870770238689
120	50	0.3998302315643443
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	21388.0
3	607.0
4	701.0
5	1863.0
6	2040.0
7	2223.0
8	2711.0
9	3767.0
10	5236.0
11	6834.0
12	9142.0
13	12329.0
14	15671.0
15	19053.0
16	21916.0
17	25843.0
18	30067.0
19	34424.0
20	39215.0
21	44193.0
22	53075.0
23	65503.0
24	85128.0
25	114606.0
26	161129.0
27	221172.0
28	296289.0
29	381456.0
30	483149.0
31	608400.0
32	767639.0
33	988035.0
34	1328231.0
35	1864067.0
36	2701320.0
37	4184004.0
38	5610224.0
39	1.3463461E7
40	48033.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	14.526479464288233	28.735644820710593	29.75214283071901	26.985732884282164
2	22.78102260990858	31.493112709129573	25.32231774024863	20.403546940713223
3	23.097125235502816	28.42319172222112	26.51496099868465	21.964722043591415
4	23.79879840476432	26.49467351847155	26.023259806043047	23.68326827072108
5	25.243980754402763	28.174208006797798	27.128527275443986	19.45328396335545
6	25.746141161946433	27.09797284404462	23.688626956981043	23.4672590370279
7	23.69212942233769	27.28951778506857	27.73353217808541	21.28482061450833
8	23.852789265755714	27.933895075290867	26.17222545366904	22.04109020528438
9	23.595501786494566	26.47624503085979	27.16754500870356	22.76070817394209
10	22.527166293679684	28.507727282862984	25.866414874755606	23.098691548701726
11	23.638640020040242	27.443608353706473	26.000369349626784	22.9173822766265
12	22.56266742086426	26.85128950056016	27.006632222103676	23.5794108564719
13	23.388744870737117	26.85172083241016	26.552946755297924	23.2065875415548
14	23.600453728343705	27.440770624155796	26.62422210034449	22.33455354715601
15	22.387768002023716	27.378753835642772	27.491299333097874	22.74217882923564
16	22.29325513805181	26.943010958182295	27.67989612181076	23.08383778195514
17	23.090525992921858	26.663611285269432	26.88143174982499	23.36443097198372
18	22.281814349378443	27.587058227585658	26.88528936916179	23.245838053874106
19	23.38892575005017	27.290317583746525	26.85991674095568	22.460839925247623
20	23.09192627006269	26.587678254926455	27.038026778651457	23.282368696359395
21	22.45807240710884	28.058552325144888	26.85495876360437	22.628416504141903
22	23.77949835013249	26.60780834493736	26.792267921404157	22.820425383525997
23	23.361842014940468	26.723484172882472	27.337356508086664	22.5773173040904
24	22.478841693765215	27.924009230709835	26.78109462900919	22.816054446515764
25	22.683781938751267	27.421905981357135	26.70321744922225	23.191094630669344
26	22.95483917002526	26.780319475143617	27.83733015595872	22.42751119887241
27	23.727124264946156	26.595040116006636	27.090113947080006	22.5877216719672
28	22.983305989725643	26.699836887458574	26.81104229937224	23.505814823443536
29	23.083676077146713	26.704356992713823	26.870253827317864	23.3417131028216
30	23.754456053276094	26.635596992728104	26.890219711005052	22.71972724299075
31	22.800544350791505	27.544295979145677	26.917780037765954	22.737379632296868
32	23.721766833944105	26.701860987830035	26.87423152949979	22.702140648726072
33	22.851876796299365	27.52461514937895	26.779757047498105	22.843751006823588
34	22.814801632486134	26.76891903202626	27.57881269101815	22.837466644469455
35	22.918785731237453	26.76983694401712	26.85337665914541	23.458000665600018
36	23.577639725018397	26.70314888373423	26.891157130191907	22.82805426105547
37	23.55157815829712	26.828025192067674	26.79666012286031	22.823736526774898
38	22.90704022716225	27.57783353074439	26.889055343098573	22.626070898994787
39	22.852435246116414	27.42905722027405	26.82050220267766	22.898005330931873
40	23.390914251140003	26.713226519959278	27.05981874953794	22.83604047936278
41	22.8417498824702	27.562162989561955	26.869402878633515	22.726684249334333
42	23.629949119805556	26.69477725494871	26.853440900298008	22.821832724947726
43	22.851870625288324	26.712497318457178	26.878316926088136	23.55731513016636
44	23.557429350954724	26.880167746765615	26.807763254518434	22.754639647761223
45	23.453748533088024	26.724651461357933	26.961339270387043	22.860260735167003
46	22.825950406877045	26.716661174623184	27.631102732901365	22.826285685598403
47	22.88746512194323	26.75583449296789	27.563940730211318	22.79275965487756
48	22.712911110911975	26.7062208528177	26.789889137223522	23.790978899046795
49	22.801033805791178	27.41769422475277	26.81203969659475	22.969232272861305
50	23.638519089591387	26.7064064284704	26.84374050834349	22.81133397359472
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	7132.0
1	4140.0
2	1148.0
3	963.5
4	779.0
5	814.0
6	849.0
7	1310.0
8	1771.0
9	2671.0
10	3571.0
11	5954.0
12	8337.0
13	13301.0
14	18265.0
15	29014.5
16	39764.0
17	61106.5
18	82449.0
19	117618.0
20	152787.0
21	207412.5
22	262038.0
23	381375.0
24	500712.0
25	517308.5
26	533905.0
27	627726.5
28	721548.0
29	811718.5
30	901889.0
31	1013772.0
32	1125655.0
33	1239374.0
34	1353093.0
35	1485529.0
36	1617965.0
37	1727229.0
38	1836493.0
39	1936113.5
40	2035734.0
41	2104269.5
42	2172805.0
43	2230245.5
44	2287686.0
45	2302492.5
46	2317299.0
47	2269768.5
48	2222238.0
49	2183255.5
50	2144273.0
51	2092793.0
52	2041313.0
53	1964489.0
54	1887665.0
55	1785709.5
56	1683754.0
57	1692340.5
58	1700927.0
59	1484913.5
60	1268900.0
61	1109345.5
62	949791.0
63	815307.0
64	680823.0
65	577034.0
66	473245.0
67	388710.0
68	304175.0
69	242916.0
70	181657.0
71	142568.5
72	103480.0
73	78622.0
74	53764.0
75	39207.5
76	24651.0
77	18027.5
78	11404.0
79	7894.0
80	4384.0
81	3054.0
82	1724.0
83	1340.5
84	957.0
85	730.5
86	504.0
87	400.5
88	297.0
89	234.0
90	171.0
91	152.5
92	134.0
93	113.5
94	93.0
95	76.5
96	60.0
97	48.5
98	37.0
99	43.0
100	49.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.20602153756667627
2	0.001286911833848177
3	7.413086600507933E-5
4	0.0014084864540965072
5	8.154395260558726E-4
6	5.841512241200251E-4
7	3.5582815682438076E-5
8	0.0
9	0.0
10	0.0
11	0.0
12	5.930469280406346E-6
13	0.0
14	0.0
15	1.4826173201015867E-5
16	8.599180456589202E-5
17	1.1860938560812693E-4
18	2.2832306729564432E-4
19	0.0
20	1.2157462024833011E-4
21	1.54192201290565E-4
22	6.642125594055108E-4
23	9.192227384629837E-5
24	0.0018592021194073895
25	0.005180264916434944
26	0.014532614971635752
27	0.00982975283227352
28	0.024193349429417692
29	0.027324637209472244
30	0.020184352195863
31	0.02704293991865294
32	0.03524774416809512
33	0.049371156759382834
34	0.05993628778242674
35	0.05698291408078438
36	0.049163590334568616
37	0.024590690871204912
38	0.015303575978088577
39	0.007294477214899806
40	0.008780059769641595
41	0.0023188134886388814
42	0.011131490839322713
43	0.036199584487600335
44	0.05649661559979105
45	0.056297944878897443
46	0.06180142037111454
47	0.07078608133093015
48	0.06879937412199402
49	0.06257238137756736
50	0.0599007049667443
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	3.3724144E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.676991086722744
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.928079559151	32.83058965664585
2	11.485310978850919	9.80317028545319
3	4.029938928872615	5.159570032426007
4	1.9034987344551497	3.2494239409572176
5	1.1805210884307114	2.5190543984322846
6	0.7647803832526796	1.9583115359625005
7	0.5529168171865995	1.6517778255140128
8	0.379097869368703	1.2943005113634976
9	0.29350960437544954	1.1273496092818718
>10	2.0328767150849063	17.503376368857072
>50	0.2855327651964647	8.52979153235402
>100	0.1589023802726477	11.726898203437983
>500	0.004458748851290482	1.2357239522287864
>1k	5.267165243399319E-4	0.3027856057139433
>5k	2.0875768508687358E-5	0.059323059228514566
>10k+	2.783435795437968E-5	1.0485534821431721
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAG	210413	0.6239239163490703	Illumina Paired End PCR Primer 2 (97% over 36bp)
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	102928	0.3052056710468322	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.001488547789381993	2.965234640203173E-6	0.0	5.930469280406346E-6	0.0
2	0.0014944782586623993	5.930469280406346E-6	2.965234640203173E-6	1.7791407841219038E-5	0.0
3	0.0014974434933026025	5.930469280406346E-6	2.965234640203173E-6	1.7791407841219038E-5	0.0
4	0.0015093044318634152	5.930469280406346E-6	2.965234640203173E-6	2.3721877121625384E-5	0.0
5	0.0015300610743448372	5.930469280406346E-6	2.965234640203173E-6	2.3721877121625384E-5	0.0
6	0.0015359915436252438	5.930469280406346E-6	2.965234640203173E-6	2.965234640203173E-5	0.0
7	0.0015419220129056501	5.930469280406346E-6	2.965234640203173E-6	2.965234640203173E-5	0.0
8	0.0017109403873972308	5.930469280406346E-6	2.965234640203173E-6	2.965234640203173E-5	0.0
9	0.0021201427677452686	5.930469280406346E-6	2.965234640203173E-6	3.5582815682438076E-5	0.0
10	0.003291410450625522	5.930469280406346E-6	2.965234640203173E-6	3.5582815682438076E-5	0.0
11	0.003463394059757306	8.895703920609519E-6	2.965234640203173E-6	3.5582815682438076E-5	0.0
12	0.003632412434248887	8.895703920609519E-6	2.965234640203173E-6	3.5582815682438076E-5	0.0
13	0.0036709604845715283	8.895703920609519E-6	5.930469280406346E-6	3.5582815682438076E-5	0.0
14	0.003733230412015795	8.895703920609519E-6	5.930469280406346E-6	4.1513284962844426E-5	0.0
15	0.006099487654897927	8.895703920609519E-6	5.930469280406346E-6	4.44785196030476E-5	0.0
16	0.006215131805865851	8.895703920609519E-6	5.930469280406346E-6	4.744375424325077E-5	0.0
17	0.006556133789489216	8.895703920609519E-6	5.930469280406346E-6	5.0408988883453946E-5	0.0
18	0.006805213499266282	8.895703920609519E-6	5.930469280406346E-6	5.0408988883453946E-5	0.0
19	0.0071610416560906635	8.895703920609519E-6	5.930469280406346E-6	5.0408988883453946E-5	0.0
20	0.06193782116456388	8.895703920609519E-6	5.930469280406346E-6	5.337422352365712E-5	0.0
21	0.0638355713342939	1.1860938560812692E-5	5.930469280406346E-6	5.633945816386029E-5	2.965234640203173E-6
22	0.06421512136823991	1.1860938560812692E-5	5.930469280406346E-6	5.930469280406346E-5	2.965234640203173E-6
23	0.0646658370335508	1.1860938560812692E-5	5.930469280406346E-6	6.226992744426663E-5	2.965234640203173E-6
24	0.06622851568893787	1.1860938560812692E-5	5.930469280406346E-6	6.523516208446981E-5	2.965234640203173E-6
25	0.06658137861112205	1.1860938560812692E-5	5.930469280406346E-6	6.523516208446981E-5	2.965234640203173E-6
26	0.06739385290253772	1.4826173201015865E-5	5.930469280406346E-6	7.116563136487615E-5	2.965234640203173E-6
27	0.06777340293648372	1.4826173201015865E-5	5.930469280406346E-6	7.413086600507933E-5	5.930469280406346E-6
28	0.0694250386310769	1.4826173201015865E-5	5.930469280406346E-6	8.006133528548567E-5	5.930469280406346E-6
29	0.07067043717996223	1.4826173201015865E-5	5.930469280406346E-6	8.302656992568885E-5	5.930469280406346E-6
30	0.08077595683377464	1.7791407841219038E-5	5.930469280406346E-6	8.302656992568885E-5	5.930469280406346E-6
31	0.7716430104200718	1.7791407841219038E-5	5.930469280406346E-6	8.599180456589202E-5	5.930469280406346E-6
32	0.7775171402423142	1.7791407841219038E-5	5.930469280406346E-6	8.599180456589202E-5	5.930469280406346E-6
33	0.7803637654969092	1.7791407841219038E-5	5.930469280406346E-6	8.599180456589202E-5	5.930469280406346E-6
34	0.780826342100781	2.3721877121625384E-5	5.930469280406346E-6	8.599180456589202E-5	5.930469280406346E-6
35	0.7812711272968115	2.3721877121625384E-5	5.930469280406346E-6	8.599180456589202E-5	5.930469280406346E-6
36	0.7817514953085244	2.3721877121625384E-5	5.930469280406346E-6	8.599180456589202E-5	5.930469280406346E-6
37	0.7823771598176072	2.668711176182856E-5	5.930469280406346E-6	8.89570392060952E-5	5.930469280406346E-6
38	0.7830769551926952	2.668711176182856E-5	5.930469280406346E-6	8.89570392060952E-5	5.930469280406346E-6
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATGCCGA	32605	0.0	37.252632	25
AATGCCG	34050	0.0	35.7672	24
AGATCGG	29730	0.0	35.626556	31
TGCCGAG	34215	0.0	35.52109	26
CGAGATC	32575	0.0	33.433167	29
CCGAGAT	33810	0.0	32.236774	28
GAGATCG	33745	0.0	31.928965	30
GCCGAGA	39500	0.0	30.888222	27
GTTCAGC	42935	0.0	28.904285	14
TATGCCG	3850	0.0	28.86905	43
TCGTATG	3915	0.0	28.2195	40
GACCGAT	4065	0.0	27.664011	32
GGAATGC	44735	0.0	27.561565	22
GAATGCC	44500	0.0	27.520987	23
CAGGAAT	47045	0.0	26.368807	20
CTCGTAT	4370	0.0	25.783665	39
AGGAATG	48090	0.0	25.700258	21
AAGCAGT	33700	0.0	24.961048	1
CGTATGC	4495	0.0	24.921925	41
GCAGGAA	50235	0.0	24.898178	19
>>END_MODULE