FastQCFastQC Report
Thu 26 May 2016
SRR522104_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR522104_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37110023
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCG764780.20608448558493211Illumina Paired End PCR Primer 2 (100% over 31bp)
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT651950.1756803007101343No Hit
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAG383710.10339794184444455Illumina Paired End PCR Primer 2 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC297250.026.86348712
ATCAACG302950.026.45209311
CAACGCA311000.025.65459613
AACGCAG331650.024.1234514
ACGCAGA333350.023.99383515
GTATCAA334300.023.9777499
AAGCAGT347200.023.8074041
GGTATCA337800.023.7423218
CGCAGAG338900.023.6076716
GTGGTAT344050.023.4004026
TGGTATC349500.022.9852397
AGAGTAC355600.022.15742319
AGTGGTA367000.022.056855
TATCAAC369550.021.76816210
CAGAGTA374200.021.18966718
GCAGAGT386650.020.6521717
AGCAGTG409000.020.1081312
AGATCGG191250.019.60001420
ATGCCGA201450.018.85933914
CGAGATC207400.018.23616418
Produced by FastQC (version 0.11.5)