##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR522104_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 37110023 Sequences flagged as poor quality 0 Sequence length 50 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.852677482846076 40.0 39.0 40.0 37.0 40.0 2 38.73619959761275 40.0 39.0 40.0 36.0 40.0 3 38.71516177718348 40.0 39.0 40.0 36.0 40.0 4 38.71274585305431 40.0 39.0 40.0 36.0 40.0 5 38.71283038547295 40.0 39.0 40.0 36.0 40.0 6 38.75819904503967 40.0 39.0 40.0 36.0 40.0 7 38.72904923826105 40.0 39.0 40.0 36.0 40.0 8 38.70843666682718 40.0 39.0 40.0 36.0 40.0 9 38.69790511851744 40.0 39.0 40.0 36.0 40.0 10 38.68392234626209 40.0 39.0 40.0 36.0 40.0 11 38.7190445556986 40.0 39.0 40.0 36.0 40.0 12 38.68068570046427 40.0 39.0 40.0 36.0 40.0 13 38.6547137144054 40.0 39.0 40.0 36.0 40.0 14 38.63767031348916 40.0 39.0 40.0 36.0 40.0 15 38.61728943148324 40.0 39.0 40.0 36.0 40.0 16 38.63242442614492 40.0 39.0 40.0 36.0 40.0 17 38.577743134247044 40.0 39.0 40.0 36.0 40.0 18 38.548688072761365 40.0 39.0 40.0 35.0 40.0 19 38.51185010044321 40.0 39.0 40.0 35.0 40.0 20 38.47468908871331 40.0 38.0 40.0 35.0 40.0 21 38.47101994520456 40.0 38.0 40.0 35.0 40.0 22 38.42219262434841 40.0 38.0 40.0 35.0 40.0 23 38.36779802049705 40.0 38.0 40.0 35.0 40.0 24 38.328340728864546 40.0 38.0 40.0 35.0 40.0 25 38.29778380897258 40.0 38.0 40.0 35.0 40.0 26 38.20320399693635 40.0 38.0 40.0 35.0 40.0 27 38.14052559331478 40.0 38.0 40.0 35.0 40.0 28 38.08928523164752 40.0 38.0 40.0 35.0 40.0 29 38.03127634817149 40.0 38.0 40.0 35.0 40.0 30 37.97461462096103 40.0 38.0 40.0 35.0 40.0 31 37.89804619630659 40.0 38.0 40.0 35.0 40.0 32 37.82434705039121 40.0 38.0 40.0 34.0 40.0 33 37.75942855114911 40.0 38.0 40.0 33.0 40.0 34 37.705751489294414 39.0 38.0 40.0 33.0 40.0 35 37.633892115884706 39.0 38.0 40.0 33.0 40.0 36 37.580777058532135 39.0 38.0 40.0 33.0 40.0 37 37.715471585668375 40.0 38.0 40.0 34.0 40.0 38 37.65546359267953 40.0 38.0 40.0 33.0 40.0 39 37.732841717721385 40.0 38.0 40.0 34.0 40.0 40 37.68640970661754 40.0 38.0 40.0 34.0 40.0 41 37.854537761941025 40.0 38.0 40.0 35.0 40.0 42 37.79380996341608 40.0 38.0 40.0 35.0 40.0 43 37.737713959379654 40.0 38.0 40.0 34.0 40.0 44 37.67612111153906 40.0 38.0 40.0 34.0 40.0 45 37.61757620037045 40.0 38.0 40.0 34.0 40.0 46 37.55845389802103 40.0 38.0 40.0 34.0 40.0 47 37.47861279956631 40.0 38.0 40.0 33.0 40.0 48 37.38440639069396 40.0 38.0 40.0 33.0 40.0 49 37.31487609156157 39.0 38.0 40.0 33.0 40.0 50 37.22634111544474 39.0 38.0 40.0 33.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1 1 -0.21668042823134925 1 2 -0.215540031786432 1 3 -0.25275445622418147 1 4 -0.22880017883131387 1 5 -0.2600766893251887 1 6 -0.2680592230613641 1 7 -0.3062459307140415 1 8 -0.2895048498656152 1 9 -0.280175778345388 1 10 -0.3102217669840357 1 11 -0.4438951859683016 1 12 -0.551284394662261 1 13 -0.494025912806741 1 14 -0.42967469598277575 1 15 -0.3905918043893166 1 16 -0.40878194702600723 1 17 -0.45327133158426847 1 18 -0.4095624431283085 1 19 -0.4082632731276874 1 20 -0.5265459929752936 1 21 -0.36159524412769883 1 22 -0.49532888575285483 1 23 -0.544775427998637 1 24 -0.5102362583580629 1 25 -0.5872757812875093 1 26 -0.43083698384178604 1 27 -0.45152919321420626 1 28 -0.41172821452563113 1 29 -0.42297398093668903 1 30 -0.41121261140278875 1 31 -0.3878168931281607 1 32 -0.385891420649493 1 33 -0.39108463904771895 1 34 -0.4497093555716418 1 35 -0.47855160817652376 1 36 -0.45175676910139373 1 37 -0.5516589392325812 1 38 -0.6093173882156151 1 39 -0.5477822878485981 1 40 -0.5829804346428205 1 41 -0.5792123312561444 1 42 -0.6063515411288449 1 43 -0.662984231970718 1 44 -0.7268278266483392 1 45 -0.6349187426417586 1 46 -0.6028460215683253 1 47 -0.6283242199813799 1 48 -0.6615706098680008 1 49 -0.7100016352303555 1 50 -0.6965943155017698 2 1 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119 35 -0.3994323777330564 119 36 -0.3705522796164189 119 37 -0.35447351343686506 119 38 -0.3896048154582985 119 39 -0.30588855517002145 119 40 -0.40914910510174707 119 41 -0.34649941801183104 119 42 -0.2953019013800784 119 43 -0.4031145902190332 119 44 -0.31267758870041007 119 45 -0.2805808328365842 119 46 -0.28079949736137877 119 47 -0.2658228840276564 119 48 -0.24388217153902048 119 49 -0.32409331648671724 119 50 -0.33794661536520465 120 1 -0.255894731913223 120 2 -0.30427040063325705 120 3 -0.31279758324021856 120 4 -0.32744521998422016 120 5 -0.3433745866587756 120 6 -0.30689462800161493 120 7 -0.31175442471423054 120 8 -0.33216414985595577 120 9 -0.32439064942084883 120 10 -0.3098491057641013 120 11 -0.29560597601042815 120 12 -0.30615194283885216 120 13 -0.31582611172562736 120 14 -0.33145170985331873 120 15 -0.3484618277304605 120 16 -0.3372133137076361 120 17 -0.35068836244826684 120 18 -0.379608203707015 120 19 -0.42691675877812685 120 20 -0.49316530872913233 120 21 -0.5222619865531684 120 22 -0.5296755070900261 120 23 -0.550711724920042 120 24 -0.5074101195744589 120 25 -0.6914288387781795 120 26 -0.7396009964707488 120 27 -0.5484009337437996 120 28 -0.7904827730668913 120 29 -0.6218175586092798 120 30 -0.6653487120767068 120 31 -0.5237417793002663 120 32 -0.5834541022701174 120 33 -0.5009401972395437 120 34 -0.4529621449856407 120 35 -0.5062474875594063 120 36 -0.3294900849778557 120 37 -0.39335400624644734 120 38 -0.38721868616516986 120 39 -0.3032159561095469 120 40 -0.3979200622389314 120 41 -0.2592307251669581 120 42 -0.2664428945063477 120 43 -0.24401822271999407 120 44 -0.2497022010436325 120 45 -0.24421035016596448 120 46 -0.19031546328409377 120 47 -0.2381937982432163 120 48 -0.20482613423829577 120 49 -0.10604759192178648 120 50 -0.1358772032146689 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 24740.0 3 1254.0 4 2055.0 5 2258.0 6 3285.0 7 5400.0 8 6099.0 9 6988.0 10 9338.0 11 11652.0 12 13619.0 13 16938.0 14 19835.0 15 22552.0 16 25261.0 17 27322.0 18 31751.0 19 39459.0 20 49967.0 21 43463.0 22 54691.0 23 69415.0 24 87669.0 25 100562.0 26 126926.0 27 158793.0 28 192676.0 29 220961.0 30 261663.0 31 317069.0 32 386137.0 33 486284.0 34 658485.0 35 957738.0 36 1511697.0 37 2722752.0 38 4918802.0 39 2.3124507E7 40 389960.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 16.475343118460028 28.43499419371001 28.233270621910794 26.856392065919167 2 24.096567094004104 29.755007466494547 25.102606363863504 21.04581907563784 3 23.77956648883621 27.425180261307037 25.87018501470355 22.925068235153205 4 25.610138919560637 25.607956153896836 25.031988969757514 23.74991595678501 5 25.362411221669856 27.854732358420513 26.524721042059163 20.25813537785046 6 25.687534604869416 26.402883124055194 23.393232604571544 24.516349666503846 7 24.232521826386858 26.254019366686833 26.98524250045399 22.528216306472327 8 24.81445780833927 26.16420367079805 25.914597250451717 23.10674127041096 9 23.64191474632069 25.74906515148212 26.5081754328204 24.10084466937679 10 23.29170208274999 27.339147701417488 25.46073603888631 23.908414176946213 11 24.083975911305686 26.576380726037275 25.715748006946804 23.623895355710232 12 23.76340214071007 26.031886318151837 26.51788170543575 23.68682983570234 13 23.47033626060858 26.59500877083138 26.24572227109952 23.688932697460523 14 23.402226904159566 27.08846032149722 26.29158872944038 23.217724044902837 15 22.986688436948 26.72142327885023 26.661989031126097 23.629899253075667 16 23.32583418372111 26.152150467180395 26.569218412052038 23.952796937046458 17 23.561639937544633 26.1305631634882 26.485855317308747 23.82194158165841 18 23.001238437846926 26.200988023224358 26.436341874812413 24.3614316641163 19 23.500187536935776 26.523675288479343 26.45250314180619 23.52363403277869 20 23.74356113980312 26.22041759445959 26.51213932149813 23.52388194423916 21 23.40593537223084 26.540587161587048 26.42831830096144 23.625159165220673 22 23.763406124508947 26.22625426579636 26.392701232598963 23.617638377095727 23 23.337207048181323 26.1405770992582 27.04830150367791 23.47391434888257 24 23.187180300318573 26.55594151686721 26.272216787393702 23.984661395420513 25 23.37551961659342 26.210688370934083 26.29722477423151 24.11656723824099 26 23.556058584822278 26.349506600165217 26.674633044143032 23.419801770869476 27 23.50600210826829 26.24398387463595 26.64418645436949 23.60582756272627 28 23.678254249178003 26.307448302215718 26.294320742880767 23.71997670572551 29 23.930401821699384 26.199031916720678 26.361645113078954 23.50892114850098 30 23.74375932346747 26.27242984540692 26.368249016812683 23.61556181431293 31 23.704141956594924 26.282703411605397 26.377264047577814 23.635890584221873 32 23.63208249933604 26.204680749075315 26.353207839682508 23.810028911906134 33 23.642900251770858 26.317434074198147 26.26028701100677 23.779378663024218 34 23.67926592393745 26.23541477417191 26.381974461937297 23.70334483995334 35 23.488487465445264 26.320391387182934 26.492992044702824 23.69812910266898 36 23.530368762240013 26.195619280035302 26.50200044479823 23.772011512926454 37 23.594382483208598 26.25270436034841 26.240835393846563 23.91207776259643 38 23.501899879301913 26.61980107855947 26.316669658180995 23.56162938395762 39 23.655048761213706 26.285799459217664 26.291222290519723 23.767929489048903 40 23.48472663309945 26.214437286901198 26.333360586083298 23.967475493916055 41 23.50910468648387 26.609093684004698 26.267728001007175 23.614073628504258 42 23.802167506352774 26.174915759294432 26.251067212779294 23.771849521573504 43 23.689786263484343 26.18111230312409 26.279235981789583 23.84986545160198 44 23.58024406230446 26.481150358322083 26.26626110720111 23.67234447217235 45 23.499858392374314 26.38150336531441 26.27451183714453 23.844126405166747 46 23.457970975191873 26.250066376018484 26.493606267559933 23.79835638122971 47 23.791619344203717 26.26755275530589 26.27106429229861 23.66976360819178 48 23.425012719294052 26.157390940189586 26.156089167234235 24.261507173282133 49 23.463778011056913 26.29057376508849 26.138708184012714 24.106940039841888 50 23.811229214570314 26.240212175030457 26.19430518245693 23.7542534279423 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 12335.0 1 7169.0 2 2003.0 3 1584.5 4 1166.0 5 1203.5 6 1241.0 7 1664.0 8 2087.0 9 3128.0 10 4169.0 11 6631.5 12 9094.0 13 14168.0 14 19242.0 15 29629.5 16 40017.0 17 60848.0 18 81679.0 19 115832.5 20 149986.0 21 203315.5 22 256645.0 23 352859.0 24 449073.0 25 480370.0 26 511667.0 27 598525.5 28 685384.0 29 772862.5 30 860341.0 31 978367.0 32 1096393.0 33 1217967.0 34 1339541.0 35 1478440.5 36 1617340.0 37 1738901.5 38 1860463.0 39 1964374.0 40 2068285.0 41 2147185.5 42 2226086.0 43 2297944.0 44 2369802.0 45 2407677.0 46 2445552.0 47 2416271.0 48 2386990.0 49 2376162.5 50 2365335.0 51 2333992.5 52 2302650.0 53 2244219.5 54 2185789.0 55 2085828.5 56 1985868.0 57 1921333.0 58 1856798.0 59 1772300.0 60 1687802.0 61 1485144.5 62 1282487.0 63 1123389.0 64 964291.0 65 835350.0 66 706409.0 67 605949.5 68 505490.0 69 424361.5 70 343233.0 71 270559.0 72 197885.0 73 159566.0 74 121247.0 75 89802.5 76 58358.0 77 43562.5 78 28767.0 79 20259.5 80 11752.0 81 8366.0 82 4980.0 83 3728.5 84 2477.0 85 1822.5 86 1168.0 87 772.5 88 377.0 89 240.0 90 103.0 91 74.0 92 45.0 93 33.5 94 22.0 95 26.0 96 30.0 97 27.5 98 25.0 99 39.5 100 54.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15851512676238438 2 6.440308592640862E-4 3 0.019078403697028158 4 0.0030665569784206277 5 1.0239821193320199E-4 6 0.0 7 2.6946897877158416E-5 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 2.3174332174356237E-4 14 7.814600384375941E-5 15 2.856371174978792E-4 16 0.0012988404776790358 17 0.0 18 9.943405316671454E-4 19 0.0 20 0.0 21 0.0 22 8.218803852533316E-4 23 2.1826987280498317E-4 24 0.01264348448396273 25 0.008342759582768245 26 0.049312823115199904 27 0.08165448994736543 28 0.033670148897509446 29 0.10074367240354444 30 0.06332251532153456 31 0.12673665009585147 32 0.16044991402996436 33 0.12867413205321915 34 0.15322006132952276 35 0.1376124180790726 36 0.17249248269126646 37 0.17200743852947759 38 0.17880344617409696 39 0.1696441955856508 40 0.1664617669463584 41 0.17199396508053902 42 0.2223011287274061 43 0.2161814882195034 44 0.24133102800825537 45 0.21050647152657384 46 0.2320370429304234 47 0.24035824499488995 48 0.22526798218368121 49 0.2579114542720709 50 0.25883573286925743 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 3.7110023E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.10494906626305 #Duplication Level Percentage of deduplicated Percentage of total 1 78.80526028517485 36.33312511601589 2 10.510739337773533 9.691942036336323 3 3.749056899629265 5.1855023221178795 4 1.872796088774793 3.4538067313783394 5 1.079249352676306 2.487936821746923 6 0.6841238285460748 1.8924896562080196 7 0.49253750681422387 1.5895891665425785 8 0.3565745088763257 1.3151879656056604 9 0.2785338872405789 1.155761161600963 >10 1.8404132019356458 16.639891702680345 >50 0.20119324413850184 6.426780888392727 >100 0.12214588508818872 10.660506292372864 >500 0.006408305172144549 1.9349573759745176 >1k 9.091908271660392E-4 0.6050580725884296 >5k 3.5086402667421354E-5 0.1086526097887826 >10k+ 2.339092994096676E-5 0.5188120806497242 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCG 76478 0.20608448558493211 Illumina Paired End PCR Primer 2 (100% over 31bp) AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 65195 0.1756803007101343 No Hit GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAG 38371 0.10339794184444455 Illumina Paired End PCR Primer 2 (97% over 36bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 2.910264970733109E-4 0.0 0.0 0.0 0.0 2 3.044999460118901E-4 0.0 0.0 0.0 0.0 3 3.23362774525901E-4 0.0 2.6946897877158416E-6 0.0 0.0 4 3.2605746431361684E-4 0.0 2.6946897877158416E-6 0.0 0.0 5 3.3144684388904854E-4 0.0 2.6946897877158416E-6 0.0 0.0 6 3.422256030399119E-4 0.0 2.6946897877158416E-6 0.0 0.0 7 3.556990519784911E-4 2.6946897877158416E-6 2.6946897877158416E-6 2.6946897877158416E-6 0.0 8 3.718671907047861E-4 2.6946897877158416E-6 2.6946897877158416E-6 5.389379575431683E-6 0.0 9 4.0420346815737626E-4 2.6946897877158416E-6 2.6946897877158416E-6 8.084069363147525E-6 2.6946897877158416E-6 10 7.006193448061189E-4 2.6946897877158416E-6 5.389379575431683E-6 8.084069363147525E-6 2.6946897877158416E-6 11 8.649954218567852E-4 2.6946897877158416E-6 5.389379575431683E-6 8.084069363147525E-6 2.6946897877158416E-6 12 0.0012179997840475604 2.6946897877158416E-6 5.389379575431683E-6 1.0778759150863367E-5 2.6946897877158416E-6 13 0.0014847740730314287 2.6946897877158416E-6 5.389379575431683E-6 1.3473448938579208E-5 2.6946897877158416E-6 14 0.0015925616645400625 2.6946897877158416E-6 5.389379575431683E-6 1.886282851401089E-5 2.6946897877158416E-6 15 0.011328475867557398 8.084069363147525E-6 5.389379575431683E-6 2.1557518301726733E-5 2.6946897877158416E-6 16 0.011738068715290206 8.084069363147525E-6 5.389379575431683E-6 2.4252208089442576E-5 2.6946897877158416E-6 17 0.012848280907829133 8.084069363147525E-6 5.389379575431683E-6 2.4252208089442576E-5 2.6946897877158416E-6 18 0.013767170125440235 8.084069363147525E-6 5.389379575431683E-6 2.4252208089442576E-5 2.6946897877158416E-6 19 0.015386678687857456 8.084069363147525E-6 5.389379575431683E-6 2.4252208089442576E-5 2.6946897877158416E-6 20 0.23053340602887798 1.0778759150863367E-5 5.389379575431683E-6 2.6946897877158416E-5 2.6946897877158416E-6 21 0.2351143786679949 1.3473448938579208E-5 5.389379575431683E-6 2.6946897877158416E-5 2.6946897877158416E-6 22 0.23622459086053382 1.3473448938579208E-5 5.389379575431683E-6 2.6946897877158416E-5 2.6946897877158416E-6 23 0.23729977208583244 1.3473448938579208E-5 5.389379575431683E-6 2.964158766487426E-5 2.6946897877158416E-6 24 0.23840190020900823 1.3473448938579208E-5 5.389379575431683E-6 2.964158766487426E-5 2.6946897877158416E-6 25 0.2394689973649437 1.3473448938579208E-5 8.084069363147525E-6 3.23362774525901E-5 2.6946897877158416E-6 26 0.24068969183877897 1.3473448938579208E-5 8.084069363147525E-6 3.5030967240305944E-5 2.6946897877158416E-6 27 0.24201547921433517 1.3473448938579208E-5 8.084069363147525E-6 3.5030967240305944E-5 2.6946897877158416E-6 28 0.2780003666394925 1.3473448938579208E-5 8.084069363147525E-6 3.772565702802178E-5 2.6946897877158416E-6 29 0.280309715787565 1.3473448938579208E-5 8.084069363147525E-6 3.772565702802178E-5 2.6946897877158416E-6 30 0.2848152371126259 1.3473448938579208E-5 8.084069363147525E-6 4.0420346815737623E-5 2.6946897877158416E-6 31 0.39308248340347296 1.616813872629505E-5 8.084069363147525E-6 4.3115036603453466E-5 2.6946897877158416E-6 32 0.3954645891758138 1.886282851401089E-5 8.084069363147525E-6 4.3115036603453466E-5 2.6946897877158416E-6 33 0.40065186701716676 1.886282851401089E-5 8.084069363147525E-6 4.3115036603453466E-5 2.6946897877158416E-6 34 0.4022740702693717 1.886282851401089E-5 8.084069363147525E-6 4.3115036603453466E-5 2.6946897877158416E-6 35 0.40424119381440426 1.886282851401089E-5 1.0778759150863367E-5 4.3115036603453466E-5 2.6946897877158416E-6 36 0.40632688371009634 1.886282851401089E-5 1.0778759150863367E-5 4.3115036603453466E-5 2.6946897877158416E-6 37 0.4086901266539231 1.886282851401089E-5 1.0778759150863367E-5 4.3115036603453466E-5 2.6946897877158416E-6 38 0.41106145366711305 1.886282851401089E-5 1.0778759150863367E-5 4.3115036603453466E-5 2.6946897877158416E-6 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCAACGC 29725 0.0 26.863487 12 ATCAACG 30295 0.0 26.452093 11 CAACGCA 31100 0.0 25.654596 13 AACGCAG 33165 0.0 24.12345 14 ACGCAGA 33335 0.0 23.993835 15 GTATCAA 33430 0.0 23.977749 9 AAGCAGT 34720 0.0 23.807404 1 GGTATCA 33780 0.0 23.742321 8 CGCAGAG 33890 0.0 23.60767 16 GTGGTAT 34405 0.0 23.400402 6 TGGTATC 34950 0.0 22.985239 7 AGAGTAC 35560 0.0 22.157423 19 AGTGGTA 36700 0.0 22.05685 5 TATCAAC 36955 0.0 21.768162 10 CAGAGTA 37420 0.0 21.189667 18 GCAGAGT 38665 0.0 20.65217 17 AGCAGTG 40900 0.0 20.108131 2 AGATCGG 19125 0.0 19.600014 20 ATGCCGA 20145 0.0 18.859339 14 CGAGATC 20740 0.0 18.236164 18 >>END_MODULE