FastQCFastQC Report
Thu 26 May 2016
SRR522103_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR522103_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34456753
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAG2328360.6757340135908918Illumina Paired End PCR Primer 2 (97% over 36bp)
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT500250.1451819908857924No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGA355650.037.9569425
AATGCCG364850.037.17963824
AGATCGG339850.036.7993431
TGCCGAG369900.036.4550126
CGAGATC360550.035.3388229
CCGAGAT369100.034.5728728
GAGATCG367000.034.43062630
GCCGAGA404700.033.61146527
GTTCAGC455900.030.21664614
GAATGCC461050.029.569123
GGAATGC472950.029.00922822
CAGGAAT486850.028.41695620
AGGAATG497150.027.73132121
GCAGGAA529100.026.39564119
TCAGCAG550900.025.34536616
AGCAGGA565750.024.75826618
CAGCAGG570350.024.5427717
TTCAGCA578450.024.06969615
TATGCCG28100.023.73290643
GATCGGA644900.021.7986261
Produced by FastQC (version 0.11.5)