##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR522103_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	34456753
Sequences flagged as poor quality	0
Sequence length	50
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.8558848827108	39.0	38.0	40.0	33.0	40.0
2	37.53885457518298	39.0	38.0	40.0	33.0	40.0
3	37.51669386839787	39.0	38.0	40.0	33.0	40.0
4	37.52109976816446	39.0	38.0	40.0	33.0	40.0
5	37.47988598925732	39.0	38.0	40.0	33.0	40.0
6	37.419686236831424	39.0	37.0	40.0	33.0	40.0
7	37.39737769255275	39.0	37.0	40.0	33.0	40.0
8	37.33379303035315	39.0	37.0	40.0	33.0	40.0
9	37.18337549101043	39.0	36.0	40.0	32.0	40.0
10	37.00823646964065	39.0	36.0	40.0	31.0	40.0
11	37.19549381219989	39.0	36.0	40.0	33.0	40.0
12	37.16251821522475	39.0	36.0	40.0	33.0	40.0
13	37.153218847986054	39.0	36.0	40.0	32.0	40.0
14	37.22043443849744	39.0	36.0	40.0	33.0	40.0
15	37.24537631273614	39.0	36.0	40.0	33.0	40.0
16	37.252556704922256	39.0	36.0	40.0	33.0	40.0
17	37.06662963860814	39.0	36.0	40.0	32.0	40.0
18	37.065161131114124	39.0	36.0	40.0	32.0	40.0
19	36.91609963945239	39.0	36.0	40.0	31.0	40.0
20	36.890085348436635	39.0	36.0	40.0	31.0	40.0
21	36.94996316106744	39.0	36.0	40.0	32.0	40.0
22	36.925451013913005	39.0	36.0	40.0	31.0	40.0
23	36.854832200817064	39.0	36.0	40.0	31.0	40.0
24	36.926705514010564	39.0	36.0	40.0	31.0	40.0
25	36.8107886718171	38.0	36.0	40.0	31.0	40.0
26	36.78861258343176	39.0	36.0	40.0	31.0	40.0
27	36.71529798527447	38.0	36.0	40.0	31.0	40.0
28	36.60155467928159	38.0	35.0	40.0	31.0	40.0
29	36.517799631323356	38.0	35.0	40.0	31.0	40.0
30	36.49860760240525	38.0	35.0	40.0	31.0	40.0
31	36.49963930147452	38.0	35.0	40.0	31.0	40.0
32	36.33100988941123	38.0	35.0	40.0	30.0	40.0
33	36.197300772942825	38.0	35.0	40.0	30.0	40.0
34	36.13396175199677	38.0	35.0	40.0	30.0	40.0
35	36.20593623548916	38.0	35.0	40.0	30.0	40.0
36	36.06845914935746	38.0	35.0	40.0	30.0	40.0
37	35.965916115195185	38.0	35.0	40.0	29.0	40.0
38	35.724378121177004	38.0	35.0	40.0	29.0	40.0
39	35.57587605541358	38.0	35.0	40.0	29.0	40.0
40	35.43772537127918	38.0	35.0	40.0	28.0	40.0
41	35.52991063899724	38.0	35.0	40.0	29.0	40.0
42	35.60746855630883	38.0	35.0	40.0	29.0	40.0
43	35.668215371309074	38.0	35.0	40.0	29.0	40.0
44	35.655912238741706	38.0	35.0	40.0	29.0	40.0
45	35.63159175213056	38.0	35.0	40.0	29.0	40.0
46	35.76627086713597	38.0	35.0	40.0	30.0	40.0
47	35.45848553983018	38.0	35.0	40.0	29.0	40.0
48	35.30726809923152	38.0	35.0	40.0	29.0	40.0
49	35.13120946132098	38.0	35.0	40.0	28.0	40.0
50	35.092845863915265	38.0	35.0	39.0	29.0	40.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1	1	-0.13564143563671394
1	2	-0.06582053428370926
1	3	-0.05406243681975553
1	4	-0.029133967163254226
1	5	-0.08150645550071545
1	6	0.12913523851973707
1	7	0.09081867152023904
1	8	-0.11655790636211094
1	9	0.05135491564260519
1	10	0.39519371258239744
1	11	0.24694586368198657
1	12	-0.3025105658476477
1	13	0.18808046253883504
1	14	0.04756106400262183
1	15	0.014720879036126178
1	16	0.11495015070774883
1	17	0.21155199783478906
1	18	0.06582321785350587
1	19	0.32001911902058566
1	20	0.2395790337810837
1	21	0.18902341201589223
1	22	0.09025275559098844
1	23	0.16287428273967208
1	24	-0.6115471319765575
1	25	-0.1272342165801632
1	26	-0.07621655573596087
1	27	-0.021739648119485366
1	28	0.05737301612752077
1	29	0.058764181911392654
1	30	0.004722388649916809
1	31	-0.014997152047541817
1	32	0.028999929287692794
1	33	0.022303107643317333
1	34	0.09564092974469673
1	35	0.2075838720305967
1	36	0.4812247818435722
1	37	0.5067621589398854
1	38	0.7520326314227148
1	39	0.7865558091389815
1	40	0.9508512049434472
1	41	0.8912139016418408
1	42	0.7137506319625615
1	43	0.3626958458794789
1	44	0.542077408063868
1	45	0.42262523672277297
1	46	0.3095985655934328
1	47	0.5581085200832661
1	48	0.5342960353706232
1	49	0.6345880085442914
1	50	0.43115654772078926
2	1	-0.1080652852802686
2	2	-0.05703893998688869
2	3	-0.10584467920472207
2	4	-0.07381964441536581
2	5	-0.053152599376282694
2	6	0.08613459043159821
2	7	0.06259443302379708
2	8	-0.15920210597325735
2	9	0.122450184599181
2	10	-0.07716532824715472
2	11	0.1796743147513311
2	12	0.16719131360795814
2	13	0.1676332817222459
2	14	0.0838215954348982
2	15	0.005971689146299752
2	16	0.18792487527029067
2	17	0.2549739032139229
2	18	0.17298459180035763
2	19	0.32374562582550936
2	20	0.3623593319016223
2	21	0.2795613251720823
2	22	0.1689630601924108
2	23	0.20772198202677572
2	24	-0.054677397692692864
2	25	0.08559792859158222
2	26	0.06506665878121254
2	27	0.012187766008835865
2	28	0.07587593252414848
2	29	0.1284012525529974
2	30	0.0214106582545881
2	31	-0.08291678911818678
2	32	-0.0443312437518415
2	33	-0.021053988921813982
2	34	-0.03919380777960413
2	35	0.187493139691
2	36	0.3915617876763591
2	37	0.5473324765030725
2	38	0.7209568051103332
2	39	0.8023043509406662
2	40	0.8995226242564769
2	41	0.8487641284726877
2	42	0.6235367628763626
2	43	0.0991508037537514
2	44	0.45507157527060826
2	45	0.3888598446294509
2	46	0.207945940836467
2	47	0.3817313327857903
2	48	0.39521632052940703
2	49	0.48413434684629664
2	50	0.424319217843923
3	1	-0.06227927813476697
3	2	0.009942156100123611
3	3	-0.004454744195797389
3	4	-0.3367835265353776
3	5	-0.10184351643583511
3	6	0.11114576984604696
3	7	0.08103974629536737
3	8	-0.7336582354077734
3	9	0.06791979842088125
3	10	-0.7902536356604912
3	11	0.13735282403840188
3	12	0.15729904713479925
3	13	0.14745275378260914
3	14	0.05191035426345536
3	15	-0.0895431509700586
3	16	0.1490220812856009
3	17	0.24467860560412902
3	18	0.06634836422436052
3	19	0.26949331566044776
3	20	0.2734183689587866
3	21	0.28407369712805064
3	22	0.19903818585751054
3	23	0.2788131183088183
3	24	0.028701748280575146
3	25	0.10772388657733245
3	26	0.10818927375116516
3	27	0.05330626695426588
3	28	0.07288182041973812
3	29	0.014931785388263563
3	30	0.03256943052387129
3	31	-0.007077230987988514
3	32	0.08464783304681589
3	33	0.10306177767659364
3	34	-0.01688565182984547
3	35	0.16524173913819595
3	36	0.41322291514627096
3	37	0.44261389468437784
3	38	0.6266895452600565
3	39	0.7512997295508725
3	40	0.8310823761603672
3	41	0.7472367131575481
3	42	0.5229932843851302
3	43	0.36483705873256156
3	44	0.43265967859340293
3	45	0.33710061806266367
3	46	0.1959758927660431
3	47	0.31329926526594676
3	48	0.31592402938736086
3	49	0.463928556608991
3	50	0.36369069950735167
4	1	-0.1189669789829324
4	2	-0.029864458821037942
4	3	0.001321430149246794
4	4	-0.13729332681123196
4	5	-0.08248879119313557
4	6	-0.04973693727323081
4	7	-0.1849260183860224
4	8	-0.6462264170900411
4	9	-0.038128520937689814
4	10	-0.31997660314912224
4	11	-0.16412512486522246
4	12	-0.07299118542637473
4	13	-1.8034728371887283
4	14	-0.29154224706513787
4	15	-0.30638460956468805
4	16	-0.4511765664160521
4	17	-0.33336200792635395
4	18	-0.040135540467801434
4	19	-0.22514440875534802
4	20	0.15787595727489645
4	21	0.09335313641950904
4	22	-0.16958255028991687
4	23	-0.041835190067828876
4	24	-0.08512373270846751
4	25	0.09722246952915015
4	26	0.14138197871211133
4	27	-0.006357305746853115
4	28	-0.009240984623467341
4	29	0.13764260154603392
4	30	0.1081995997052374
4	31	-0.002394719912281573
4	32	0.0869668719440142
4	33	0.09953990870612017
4	34	0.04852724387943397
4	35	0.21969043862340243
4	36	0.15738613908990118
4	37	0.2002761919605902
4	38	-0.1267939196116643
4	39	0.08707085873684406
4	40	0.3821358901843297
4	41	-0.09252325578862752
4	42	-0.14347622420658723
4	43	0.09932634782724392
4	44	0.11706013918163194
4	45	0.12565051539259287
4	46	0.1646821247879089
4	47	0.36861088266891784
4	48	0.37050366923476474
4	49	0.15446197344840584
4	50	0.35786027339256776
5	1	-0.010864188708524125
5	2	-0.07096577493243927
5	3	-0.1390722596436902
5	4	-0.18873223062781364
5	5	-0.04933982030390638
5	6	0.09056606503028775
5	7	0.0035151465074463317
5	8	0.03372642752930943
5	9	-0.14720518856136522
5	10	-0.7228390110510929
5	11	0.20838091013667537
5	12	0.2006782451823952
5	13	-0.5071610059261857
5	14	-0.1511023134895879
5	15	-0.03386924224312082
5	16	-0.0645182395501962
5	17	-0.4713854536661657
5	18	-0.31216049777586363
5	19	-0.03266978259930653
5	20	-0.060988269199086176
5	21	0.22075181465191207
5	22	0.17578823023534795
5	23	0.07153221754472128
5	24	-0.0869065938563196
5	25	0.07973165796661874
5	26	-0.5927336477543221
5	27	0.0488661963733108
5	28	-0.26779279720485505
5	29	0.020685842752648398
5	30	0.06415167838407143
5	31	-0.039824736804099814
5	32	-0.05725433392347412
5	33	-0.31057902428490536
5	34	-0.10353015112516317
5	35	0.009501857561240001
5	36	0.27755359568433136
5	37	0.0507765560630844
5	38	0.5309584181955174
5	39	0.12253968476785815
5	40	-0.06756289520212277
5	41	0.3429779187608446
5	42	0.0100293697767313
5	43	0.3051893764250835
5	44	0.15761176168162194
5	45	-1.5671833943365812
5	46	0.11845753197042797
5	47	0.2739814617178027
5	48	0.3116365791539337
5	49	0.2831146868851704
5	50	-1.2084477009185335
6	1	-0.06614697241512602
6	2	-0.14327690351684197
6	3	-0.12601954300241403
6	4	-0.14447669402279217
6	5	-0.3140546348845774
6	6	0.18473962866652016
6	7	-0.30567819704645416
6	8	0.12488337544218808
6	9	-0.037781116034615536
6	10	0.3346509503710067
6	11	0.2937136565656644
6	12	-0.07165744045376243
6	13	-1.5489587732024148
6	14	0.05682120031047333
6	15	-0.2975763985294577
6	16	-0.08260714211448317
6	17	-0.16957494676904616
6	18	-0.02921441444113526
6	19	0.016483416756777558
6	20	-2.623151947801354
6	21	-0.23051324394214845
6	22	-0.21353826221746885
6	23	-0.14264314674953482
6	24	-1.9333675519120916
6	25	-0.39046865990275137
6	26	0.02156459421012613
6	27	-0.3352165223042789
6	28	-0.2239555812018068
6	29	0.05805524408484786
6	30	-0.14571890515438923
6	31	-0.3010056788868809
6	32	0.03777296591303525
6	33	0.036851232370196385
6	34	0.0878407129211567
6	35	-0.947219810868873
6	36	0.1697786991592949
6	37	0.17440033445834047
6	38	0.46528474191354974
6	39	-1.6822968199625166
6	40	0.010170163198914395
6	41	0.52060402871669
6	42	-0.535670941270304
6	43	-0.2617383733494947
6	44	-0.3927352871060066
6	45	0.024805870685938203
6	46	-0.4833414561660021
6	47	-0.10357470732734697
6	48	-0.13876298707351253
6	49	-0.22591440910739635
6	50	-0.3125565900217211
7	1	-0.1507693150579783
7	2	-0.19364640538771738
7	3	-0.11482162331524393
7	4	-0.3240639329293842
7	5	-0.3503873279152643
7	6	-0.5622198747941738
7	7	-0.09830918824562929
7	8	-0.8088596052688501
7	9	0.11951602791348392
7	10	0.2620828236324755
7	11	-0.0038306325230621496
7	12	0.1767692161027128
7	13	0.009374769396096383
7	14	-0.18760080089718656
7	15	-0.2132859891214096
7	16	-0.03355113601882209
7	17	-0.21530305321902432
7	18	-0.6211271208403204
7	19	-0.49857154468711684
7	20	0.17556409265493755
7	21	-0.19624984607038698
7	22	0.11408604304372716
7	23	0.07283812220777719
7	24	-0.3215909105950061
7	25	-0.638006103486461
7	26	-0.295711356937268
7	27	-1.5048224687295289
7	28	-0.593296914343135
7	29	-0.08519783111668033
7	30	-0.0919209122127711
7	31	-0.36781115161534217
7	32	-0.6867629760738367
7	33	-0.20955668824509388
7	34	-0.14410943914499796
7	35	-0.8408530756675745
7	36	-1.4694941467255873
7	37	-0.7860216749923126
7	38	0.06274896483139258
7	39	-0.023277305735049936
7	40	-0.277810544345904
7	41	0.23854432604831288
7	42	-0.5883802669381737
7	43	0.05314016910363506
7	44	0.11051123897755133
7	45	0.02771270702233153
7	46	0.023887607720027404
7	47	0.2653003814958623
7	48	0.09353584882578048
7	49	0.22632331880743806
7	50	0.27046126826692785
8	1	-0.3349412723472227
8	2	-0.31432226991042
8	3	-0.4290624291581011
8	4	-0.3396323339399885
8	5	-0.47218616612146036
8	6	-2.011202134196701
8	7	-0.40079156854381637
8	8	-1.598233410123349
8	9	-0.4789568864220044
8	10	-0.24129174960934563
8	11	-1.804165561727089
8	12	-0.26698538657485926
8	13	-0.08098079779230005
8	14	-0.33741427576863003
8	15	-0.2744876456761389
8	16	-0.24567085940523725
8	17	0.01180805460001011
8	18	0.039883007366491086
8	19	0.2901695772157922
8	20	0.23003389025886634
8	21	0.2193241834916435
8	22	0.20414541559330246
8	23	0.27328260103391244
8	24	-0.20786994650474355
8	25	0.15029369383053393
8	26	0.02615523101673034
8	27	0.046909047611343624
8	28	0.07947050131524946
8	29	0.10498056953560564
8	30	0.15494597095875662
8	31	0.07641889142405489
8	32	0.07902525870695598
8	33	0.15147969377697734
8	34	0.16744480731956912
8	35	0.042426553592797234
8	36	-0.2664954368908852
8	37	-0.26082670056377566
8	38	0.03176412469300516
8	39	0.44160109847336315
8	40	-0.2705609126980093
8	41	-0.26404677422350886
8	42	0.06888218711272032
8	43	-0.27464072507798676
8	44	0.1066045447353332
8	45	-0.13456972371442788
8	46	0.04027779637590356
8	47	0.31007640865781383
8	48	0.39546785473130086
8	49	0.2845585002414879
8	50	0.25443434969722034
9	1	0.056495900045597125
9	2	-0.008044365104844076
9	3	0.2333482677537546
9	4	0.12521347243139758
9	5	0.13959635708794593
9	6	0.21422307374231053
9	7	0.2142124148688822
9	8	-0.1614389071834097
9	9	-0.5503630915721942
9	10	0.18633481007433517
9	11	0.22235622820978307
9	12	-0.14746238796043798
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115	14	-0.4606577200344475
115	15	-0.37337303128227006
115	16	-0.22502883095852155
115	17	-0.11226880917345028
115	18	-0.2859590202133191
115	19	-0.5319165285497007
115	20	-0.36265360686707737
115	21	-0.21630337936313992
115	22	-0.2852343398507813
115	23	-0.3944340418517669
115	24	-0.3648411618815359
115	25	-0.30426379936292136
115	26	-1.814580671546679
115	27	-0.3734806684542349
115	28	-0.41033688453344297
115	29	-0.28379047997376716
115	30	-1.3233185998320636
115	31	-0.4113051763338902
115	32	-0.43293105123617437
115	33	-0.3517032995274221
115	34	-0.4813818519665318
115	35	-0.13542988485303198
115	36	0.012195322170995837
115	37	0.2615822542969468
115	38	0.3448110215728093
115	39	0.47915258045225784
115	40	0.2915756930823932
115	41	-0.18911537644643772
115	42	0.32559889202499903
115	43	-1.0799698343223483
115	44	0.11910327400705967
115	45	-0.01602262626936124
115	46	0.20324061302169127
115	47	0.34666892021599693
115	48	0.36372735287139335
115	49	0.4606520755880865
115	50	0.38502315983782154
116	1	-0.31912678163664054
116	2	-0.4448892066004575
116	3	-0.42982809998274973
116	4	-0.3606350583674498
116	5	-0.38765722148660586
116	6	-0.2587482642064458
116	7	-0.25298181619536564
116	8	-0.3380413088897143
116	9	-0.10087877376991372
116	10	-0.11814339671585827
116	11	-0.11717332720481721
116	12	-0.5180129388418706
116	13	-0.2737690946951261
116	14	-0.4445230230840025
116	15	-0.25356714047027396
116	16	-0.16187516428764326
116	17	-0.5078499152679683
116	18	-0.25976398397601486
116	19	-0.06682705840579217
116	20	-0.06703498902400895
116	21	0.04757090847600409
116	22	-0.06055577787566335
116	23	-0.4987633351364025
116	24	-0.15632006553115474
116	25	-0.07935467842447252
116	26	-0.2598963238962497
116	27	-0.29397873708905564
116	28	-0.1834591998622983
116	29	-0.5903720923372973
116	30	-0.24835199030200528
116	31	-0.3020708261155747
116	32	-0.2405756861225541
116	33	-0.7064306206570592
116	34	-0.1764452174457034
116	35	0.06816475707199032
116	36	0.1516476494536505
116	37	0.23999841713461478
116	38	0.5699115930576255
116	39	0.18705686307163205
116	40	0.49212601249030286
116	41	0.7228581104370733
116	42	0.400819479569023
116	43	0.3819070661170727
116	44	0.24733037084654796
116	45	0.2989683422867273
116	46	0.21749052923841816
116	47	0.32564504057675947
116	48	0.4633006695658324
116	49	0.5105446996095822
116	50	0.3127859806287887
117	1	-1.3249574186733568
117	2	-1.0279438185720764
117	3	-0.9440088361131984
117	4	-0.8647519546394449
117	5	-0.8881754732218141
117	6	-0.697848201660122
117	7	-0.8584508061959895
117	8	-0.7236332474799099
117	9	-0.5051083101136413
117	10	-0.5149546686598967
117	11	-0.27386532783079787
117	12	-0.2763432942792363
117	13	-0.30621633259807624
117	14	-0.4213332018388414
117	15	-0.5588123021520701
117	16	-0.28095527190251346
117	17	-0.5779695162511942
117	18	-0.33482090783519425
117	19	-0.3423015343170448
117	20	-0.40737007035090755
117	21	-0.1299582886694992
117	22	-0.19029849955010292
117	23	-0.22072030496759254
117	24	-0.3562819035671154
117	25	-0.2589113048185183
117	26	-0.31110059654764655
117	27	-0.3170406388427338
117	28	-0.22833926144986094
117	29	-0.30668082529496843
117	30	-0.262914294754097
117	31	-0.3597051456954077
117	32	-0.5520314093065224
117	33	-0.23074348041221526
117	34	-1.2881266644322125
117	35	-0.06391307472745922
117	36	0.18557755624045313
117	37	0.43748514412163786
117	38	0.5223265487373538
117	39	0.5092296710542215
117	40	0.7843553957120477
117	41	0.4530335831287502
117	42	0.602677168830148
117	43	0.3326766028735335
117	44	0.27505879127679833
117	45	0.023005934102506842
117	46	0.2743211107482182
117	47	0.455764789689141
117	48	0.6385475983681985
117	49	0.6086899130044756
117	50	0.547861738941215
118	1	-0.5346124149651956
118	2	-0.699658535949979
118	3	-0.5108963311010442
118	4	-0.5702092085546155
118	5	-0.5395309032423157
118	6	-0.36568759075193213
118	7	-0.35266237285298274
118	8	-0.3131319598093256
118	9	-0.37058776035800634
118	10	0.027178542036061515
118	11	-0.7764280531676846
118	12	-0.3356525658622189
118	13	-0.3219941986065251
118	14	-0.3833791704419838
118	15	-0.4605080745977972
118	16	-0.32682073899082553
118	17	-0.12852729049246392
118	18	-0.20999972416223045
118	19	-0.0830392622942071
118	20	-0.12548120927861106
118	21	-0.20037280706768712
118	22	-0.22513000943938977
118	23	-0.4950483909190808
118	24	-0.30230846756651175
118	25	-1.0478941216468272
118	26	-0.7086508991670044
118	27	-0.9128786219831895
118	28	-0.8024403549277821
118	29	-0.6991912658070518
118	30	-0.8618899390715455
118	31	-0.45165606579267603
118	32	-0.5586373192220933
118	33	-0.2703211959554821
118	34	-0.5196359080461548
118	35	-0.25424451931809244
118	36	-0.04484100568028282
118	37	-0.08058474912738234
118	38	-0.05539494682052748
118	39	-0.8505757979815343
118	40	0.3043363611592156
118	41	0.43015719289673626
118	42	0.28578379133081455
118	43	0.1128186194966716
118	44	-0.8832969114897935
118	45	0.05180458166124424
118	46	-0.4830402903619344
118	47	0.10910766955591811
118	48	0.14311456775595133
118	49	0.11634943730846459
118	50	0.12491281482877525
119	1	-0.4668561031417795
119	2	-0.6100830907567385
119	3	-0.5218143685943772
119	4	-0.4530024700343347
119	5	-0.4202259894935949
119	6	-0.2840178107391722
119	7	-0.4322907550686139
119	8	-0.4152220681065728
119	9	-0.3184933409644728
119	10	-0.02265122203075265
119	11	-0.20827854981184402
119	12	-1.0334384007768307
119	13	-0.38692343006874097
119	14	-0.48891468789540227
119	15	-0.6334758245084728
119	16	-0.3649288101836632
119	17	-0.17305157005324645
119	18	-0.22897883298905697
119	19	-0.18874676888789566
119	20	-0.21731242896815672
119	21	-0.0797164295731605
119	22	-0.30341142697879775
119	23	-0.1875284497316514
119	24	-0.3189833623242322
119	25	-0.26700471793269287
119	26	-0.48337689597025246
119	27	-0.40101124785095976
119	28	-1.1978785922072959
119	29	-0.5503894776911196
119	30	-0.4851991750105995
119	31	-0.6690125869082948
119	32	-0.9509480540372977
119	33	-0.527481459321649
119	34	-0.6328309724696339
119	35	-0.7730345828721141
119	36	-0.22616033476298725
119	37	-0.0835639208316934
119	38	-0.06326227997290346
119	39	-0.2926395613048669
119	40	-1.4440972316828393
119	41	0.24608211051988604
119	42	0.059377391202680485
119	43	-0.19039328325609972
119	44	-0.564547908230054
119	45	-0.352358139172253
119	46	-0.11328004636065003
119	47	-0.22810802677165753
119	48	-0.010793017455817733
119	49	-0.08162512446950387
119	50	-0.06402944748965211
120	1	-0.4455600922940448
120	2	-0.5056587224114111
120	3	-0.48316088511417377
120	4	-0.5132897883608081
120	5	-0.5440563664388662
120	6	-0.41373769248579606
120	7	-0.4579108257788036
120	8	-0.4384952834131468
120	9	-0.2810854676647523
120	10	-0.8142778532045156
120	11	-0.3978489900648867
120	12	-0.3559782763166197
120	13	-0.3209767043529581
120	14	-0.4345847542288723
120	15	-0.5567216470226484
120	16	-0.402014456899245
120	17	-0.1925698678166725
120	18	-0.1880322363074356
120	19	-0.10067000835923778
120	20	-0.11886579857546309
120	21	-0.1444678734321414
120	22	-0.2816224656257944
120	23	-0.40485264032577106
120	24	-0.3840213320188397
120	25	-0.26403817858810896
120	26	-0.4279512239454917
120	27	-0.6884652129544406
120	28	-0.6586459047423787
120	29	-0.4885034132794459
120	30	-0.43436548621211557
120	31	-0.39633486010915675
120	32	-0.7810459644510175
120	33	-0.46215822136072404
120	34	-0.7527783786548312
120	35	-0.4277570743527903
120	36	0.08713533097751025
120	37	0.04918066284006528
120	38	0.28154314347115417
120	39	0.04214446339759803
120	40	0.475858559556805
120	41	0.5779854527562875
120	42	0.4346138700568716
120	43	0.2510824194927892
120	44	-0.05598339131287844
120	45	0.12076532062611989
120	46	0.046746824696022315
120	47	0.008248096655592008
120	48	0.14091165873588807
120	49	-0.3681675595672118
120	50	0.18541552507342374
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	22965.0
3	914.0
4	1090.0
5	1324.0
6	2239.0
7	3234.0
8	3966.0
9	5412.0
10	7613.0
11	9820.0
12	13256.0
13	17781.0
14	22046.0
15	27083.0
16	31419.0
17	36537.0
18	42381.0
19	48479.0
20	55560.0
21	63511.0
22	75142.0
23	92148.0
24	117723.0
25	157376.0
26	218264.0
27	297923.0
28	396751.0
29	510769.0
30	647932.0
31	815056.0
32	1021253.0
33	1299487.0
34	1708825.0
35	2331357.0
36	3232601.0
37	4644056.0
38	5588389.0
39	1.0867466E7
40	17605.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	13.752060747710033	28.81417853757982	30.40475496174765	27.029005752962497
2	22.679178271416216	31.82029436704978	25.262695464922984	20.23783189661102
3	22.55083544083231	29.054278209135347	26.75468027005125	21.64020607998109
4	22.933673250239785	27.06623678063298	26.744826426916163	23.255263542211075
5	24.794843630157366	28.18836042186813	27.82178960899384	19.195006338980665
6	24.87507866035741	27.537972822282402	24.46300475295315	23.123943764407038
7	23.497661125225427	27.691699795163444	27.950990140100313	20.85964893951082
8	23.236127327493687	28.517446202780626	26.49418823648299	21.752238233242696
9	22.99102297886281	26.86490511743808	27.719164368157383	22.424907535541728
10	22.21057219175585	28.868169905620533	26.092748785702476	22.828509116921143
11	23.268419981418447	27.439698105041995	26.481015201867685	22.810866711671874
12	22.037076447685017	27.179046731420108	27.229965632571357	23.55391118832352
13	23.165601239327454	27.146931111007472	26.91846210813886	22.769005541526212
14	23.38031676983609	27.53568509487821	26.96967703253989	22.11432110274581
15	22.098431619485446	27.65443975524914	27.810455616639214	22.436673008626205
16	22.0063093588339	27.42873602414621	28.060478716071596	22.50447590094829
17	22.55436293639783	27.13938355412995	27.12033358458552	23.185919924886704
18	21.965986278272805	28.080450208567314	27.22670725011399	22.726856263045885
19	23.128488286647165	27.50772678927527	27.126477765862035	22.23730715821553
20	22.5911642951263	27.093441485244142	27.164524863786365	23.150869355843195
21	22.153555792096835	28.301009674359044	27.12275587894193	22.42267865460219
22	23.268276197022722	27.03457258667307	27.10215608691911	22.594995129385094
23	23.22743444917608	27.119198779617466	27.290886539836894	22.36248023136956
24	22.211906381491296	28.174921199907892	27.074468329017805	22.538704089583007
25	22.389346265272923	27.90152282493084	27.015519761269	22.69361114852723
26	22.645524864290262	27.108702234560855	28.04163266860446	22.20414023254442
27	23.474389385687633	27.02619903478493	27.148483565587156	22.35092801394028
28	22.581672372330836	27.03816885408386	27.030810296233476	23.349348477351825
29	22.688071458536673	27.087899217387335	27.049895966427083	23.17413335764891
30	23.44514806508131	27.02362084406009	27.09276307888842	22.438468011970176
31	22.502530578347866	27.896487828098852	27.126705475109354	22.474276118443925
32	23.533608233582605	27.051439335882122	27.017386490418343	22.39756594011693
33	22.55967565383385	27.94417672345632	26.958795332790892	22.53735228991894
34	22.518916647058845	27.105660006221655	27.84732492553456	22.528098421184936
35	22.66149971196934	27.11443223810829	27.008265396834467	23.215802653087906
36	23.432983310772055	27.03347502678347	27.009445539617904	22.524096122826567
37	23.349703727508853	27.121646921457238	27.00804991417083	22.520599436863083
38	22.64467187699776	28.007487365650967	27.04531327801402	22.302527479337254
39	22.53557994290399	27.88106862059555	27.057883146385304	22.52546829011516
40	23.20151747808544	27.05774922296611	27.22018399561098	22.52054930333747
41	22.61895353803755	27.966502671615302	27.04488993407006	22.36965385627709
42	23.40615690370576	27.054004235049206	27.044977683083797	22.494861178161234
43	22.57164652326427	27.055563019874146	27.04368333426428	23.32910712259731
44	23.401471318209186	27.179862125076422	26.99511617362606	22.423550383088333
45	23.293356253930778	27.107917787156826	27.085682701347167	22.51304325756523
46	22.56707381119753	27.042376362914016	27.89389134520724	22.496658480681216
47	22.693173895750345	27.064976004059034	27.810950699301358	22.430899400889263
48	22.50540395515963	27.047520186634667	26.95582981830838	23.49124603989732
49	22.508613308698823	27.82718696034329	27.049837374831004	22.614362356126875
50	23.498742795004798	27.04692985460878	26.985809894504325	22.468517455882093
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	6826.0
1	3873.0
2	920.0
3	758.5
4	597.0
5	663.5
6	730.0
7	1191.5
8	1653.0
9	2644.0
10	3635.0
11	6267.5
12	8900.0
13	14725.0
14	20550.0
15	32430.0
16	44310.0
17	67599.0
18	90888.0
19	129421.5
20	167955.0
21	228358.0
22	288761.0
23	383168.0
24	477575.0
25	529935.0
26	582295.0
27	681285.5
28	780276.0
29	879259.0
30	978242.0
31	1107043.0
32	1235844.0
33	1367636.0
34	1499428.0
35	1643832.5
36	1788237.0
37	1898268.0
38	2008299.0
39	2097552.0
40	2186805.0
41	2246501.5
42	2306198.0
43	2345444.0
44	2384690.0
45	2377824.5
46	2370959.0
47	2318549.0
48	2266139.0
49	2217609.0
50	2169079.0
51	2101862.0
52	2034645.0
53	1942512.5
54	1850380.0
55	1737671.0
56	1624962.0
57	1634893.5
58	1644825.0
59	1410842.0
60	1176859.0
61	1017175.5
62	857492.0
63	727714.0
64	597936.0
65	500398.0
66	402860.0
67	331177.5
68	259495.0
69	207107.0
70	154719.0
71	122677.5
72	90636.0
73	69329.5
74	48023.0
75	35600.5
76	23178.0
77	17314.5
78	11451.0
79	8201.5
80	4952.0
81	3498.5
82	2045.0
83	1533.5
84	1022.0
85	794.5
86	567.0
87	435.5
88	304.0
89	226.0
90	148.0
91	140.5
92	133.0
93	125.0
94	117.0
95	96.5
96	76.0
97	66.0
98	56.0
99	68.5
100	81.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.21712724933774227
2	9.141894478565638E-4
3	9.867441659404181E-5
4	0.0014191702857201897
5	3.163385708456046E-4
6	2.0315321063479197E-4
7	3.772845340360422E-5
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	8.706566170062512E-6
17	1.4510943616770855E-5
18	3.482626468025005E-5
19	2.6119698510187542E-5
20	9.867441659404181E-5
21	0.0
22	3.4245826935579214E-4
23	4.6435019573666735E-5
24	0.0016687585159286481
25	0.00553157170671305
26	0.011054436847256037
27	0.010154758343016244
28	0.02051266989666728
29	0.02815123061653546
30	0.021696762895795783
31	0.02639250425018283
32	0.03839305462125233
33	0.050785400469974644
34	0.0550371069496885
35	0.056438864103068566
36	0.04566593956197788
37	0.030249513063520524
38	0.011748059952137685
39	0.004225586781203673
40	0.004910503319915258
41	0.001834183273159836
42	0.008253824729219263
43	0.03307624488006749
44	0.05098565149188607
45	0.058586483758350655
46	0.06114911640107239
47	0.0722296729468386
48	0.0712922659891952
49	0.05952098852727069
50	0.06625696855417573
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	3.4456753E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.60029568883308
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.63305750448751	36.953564924417876
2	11.655739724316948	11.096333146991288
3	3.8698633044047663	5.526199126950946
4	1.8397009550806105	3.5028123776346267
5	1.0850397549190647	2.582410658414323
6	0.7105976109803579	2.029479383906605
7	0.4880704360140733	1.6262607949873304
8	0.34958211633673286	1.3312169684125224
9	0.2748653560613295	1.1775305000822107
>10	1.787638292262753	16.828540833557454
>50	0.1901277989290448	6.249001504512997
>100	0.11400555696703303	9.631316002954831
>500	0.0016002621481649014	0.4597502831786581
>1k	6.852663468259903E-5	0.056334681309589144
>5k	1.8343053120597866E-5	0.05513263868977298
>10k+	2.4457403983620194E-5	0.894116173998783
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAG	232836	0.6757340135908918	Illumina Paired End PCR Primer 2 (97% over 36bp)
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	50025	0.1451819908857924	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.094596319043759E-4	2.902188723354171E-6	0.0	0.0	2.902188723354171E-6
2	6.181661980744384E-4	1.1608754893416684E-5	0.0	0.0	2.902188723354171E-6
3	6.239705755211467E-4	1.1608754893416684E-5	0.0	2.902188723354171E-6	2.902188723354171E-6
4	6.355793304145635E-4	1.7413132340125025E-5	0.0	8.706566170062512E-6	2.902188723354171E-6
5	6.471880853079801E-4	2.0315321063479196E-5	0.0	1.1608754893416684E-5	2.902188723354171E-6
6	6.587968402013969E-4	2.0315321063479196E-5	0.0	1.7413132340125025E-5	5.804377446708342E-6
7	6.675034063714593E-4	2.3217509786833368E-5	0.0	2.0315321063479196E-5	5.804377446708342E-6
8	7.777865778589178E-4	2.3217509786833368E-5	0.0	2.0315321063479196E-5	5.804377446708342E-6
9	0.0011057339035979391	2.3217509786833368E-5	0.0	2.0315321063479196E-5	5.804377446708342E-6
10	0.002057651804858107	2.3217509786833368E-5	0.0	2.3217509786833368E-5	5.804377446708342E-6
11	0.002167934976345566	2.902188723354171E-5	0.0	2.3217509786833368E-5	5.804377446708342E-6
12	0.002257902826769545	3.192407595689588E-5	0.0	2.611969851018754E-5	5.804377446708342E-6
13	0.002292729091449795	3.772845340360422E-5	0.0	2.611969851018754E-5	5.804377446708342E-6
14	0.00234496848847017	4.063064212695839E-5	0.0	3.192407595689588E-5	5.804377446708342E-6
15	0.0038628131907844014	4.063064212695839E-5	0.0	3.772845340360422E-5	5.804377446708342E-6
16	0.00393827009759161	4.063064212695839E-5	0.0	3.772845340360422E-5	5.804377446708342E-6
17	0.004216880215033611	4.063064212695839E-5	0.0	4.3532830850312564E-5	5.804377446708342E-6
18	0.004393913727158215	4.063064212695839E-5	0.0	4.3532830850312564E-5	5.804377446708342E-6
19	0.004649306334813382	4.063064212695839E-5	0.0	4.3532830850312564E-5	5.804377446708342E-6
20	0.043114915674149566	4.063064212695839E-5	0.0	4.3532830850312564E-5	5.804377446708342E-6
21	0.04457471660199671	4.063064212695839E-5	0.0	4.3532830850312564E-5	5.804377446708342E-6
22	0.04484462015326865	4.063064212695839E-5	0.0	4.3532830850312564E-5	8.706566170062512E-6
23	0.0451580565353909	4.063064212695839E-5	0.0	4.6435019573666735E-5	8.706566170062512E-6
24	0.04704157701684776	4.063064212695839E-5	0.0	5.223939702037508E-5	8.706566170062512E-6
25	0.047404350607267026	4.3532830850312564E-5	0.0	5.223939702037508E-5	8.706566170062512E-6
26	0.04824018095959303	4.3532830850312564E-5	0.0	5.223939702037508E-5	8.706566170062512E-6
27	0.04863778081469255	4.3532830850312564E-5	0.0	5.223939702037508E-5	8.706566170062512E-6
28	0.05001632045828578	4.3532830850312564E-5	0.0	5.223939702037508E-5	8.706566170062512E-6
29	0.051438392932729324	4.3532830850312564E-5	0.0	5.223939702037508E-5	8.706566170062512E-6
30	0.062188099964033176	4.3532830850312564E-5	0.0	5.514158574372925E-5	8.706566170062512E-6
31	0.8267348928670093	4.3532830850312564E-5	0.0	5.514158574372925E-5	8.706566170062512E-6
32	0.8326843797498853	4.3532830850312564E-5	0.0	5.804377446708342E-5	8.706566170062512E-6
33	0.834025190940075	4.6435019573666735E-5	0.0	5.804377446708342E-5	8.706566170062512E-6
34	0.8343647470207074	4.6435019573666735E-5	0.0	6.094596319043759E-5	8.706566170062512E-6
35	0.8347188140449566	4.9337208297020906E-5	0.0	6.384815191379176E-5	8.706566170062512E-6
36	0.835099000767716	5.223939702037508E-5	0.0	6.384815191379176E-5	8.706566170062512E-6
37	0.835574959718346	5.223939702037508E-5	0.0	6.384815191379176E-5	8.706566170062512E-6
38	0.8359435376862121	5.223939702037508E-5	0.0	6.384815191379176E-5	1.1608754893416684E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATGCCGA	35565	0.0	37.95694	25
AATGCCG	36485	0.0	37.179638	24
AGATCGG	33985	0.0	36.79934	31
TGCCGAG	36990	0.0	36.45501	26
CGAGATC	36055	0.0	35.33882	29
CCGAGAT	36910	0.0	34.57287	28
GAGATCG	36700	0.0	34.430626	30
GCCGAGA	40470	0.0	33.611465	27
GTTCAGC	45590	0.0	30.216646	14
GAATGCC	46105	0.0	29.5691	23
GGAATGC	47295	0.0	29.009228	22
CAGGAAT	48685	0.0	28.416956	20
AGGAATG	49715	0.0	27.731321	21
GCAGGAA	52910	0.0	26.395641	19
TCAGCAG	55090	0.0	25.345366	16
AGCAGGA	56575	0.0	24.758266	18
CAGCAGG	57035	0.0	24.54277	17
TTCAGCA	57845	0.0	24.069696	15
TATGCCG	2810	0.0	23.732906	43
GATCGGA	64490	0.0	21.798626	1
>>END_MODULE