##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR522102_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	40598193
Sequences flagged as poor quality	0
Sequence length	50
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.4544909671226	40.0	38.0	40.0	35.0	40.0
2	38.27321321912037	40.0	38.0	40.0	35.0	40.0
3	38.24439297088912	40.0	38.0	40.0	35.0	40.0
4	38.24456773236188	40.0	38.0	40.0	35.0	40.0
5	38.25280814838237	40.0	38.0	40.0	35.0	40.0
6	38.29703156985337	40.0	38.0	40.0	35.0	40.0
7	38.26158622380065	40.0	38.0	40.0	35.0	40.0
8	38.23273006264097	40.0	38.0	40.0	35.0	40.0
9	38.225938331787326	40.0	38.0	40.0	35.0	40.0
10	38.2082578897046	40.0	38.0	40.0	35.0	40.0
11	38.25665174309605	40.0	38.0	40.0	35.0	40.0
12	38.1989161931419	40.0	38.0	40.0	35.0	40.0
13	38.17269128209721	40.0	38.0	40.0	35.0	40.0
14	38.14054598439886	40.0	38.0	40.0	35.0	40.0
15	38.12686766625303	40.0	38.0	40.0	35.0	40.0
16	38.130008323276854	40.0	38.0	40.0	35.0	40.0
17	38.07027536915251	40.0	38.0	40.0	35.0	40.0
18	38.02266753596644	40.0	38.0	40.0	34.0	40.0
19	37.974041332332206	40.0	38.0	40.0	34.0	40.0
20	37.918251928109214	40.0	38.0	40.0	33.0	40.0
21	37.92557136717883	40.0	38.0	40.0	34.0	40.0
22	37.87116138395618	40.0	38.0	40.0	33.0	40.0
23	37.79224198476026	39.0	38.0	40.0	33.0	40.0
24	37.753688323024626	39.0	38.0	40.0	33.0	40.0
25	37.71569724790461	39.0	38.0	40.0	33.0	40.0
26	37.60986573959092	39.0	38.0	40.0	33.0	40.0
27	37.52277380916929	39.0	38.0	40.0	33.0	40.0
28	37.489059451488394	39.0	38.0	40.0	33.0	40.0
29	37.41062381766597	39.0	38.0	40.0	33.0	40.0
30	37.33393528130673	39.0	38.0	40.0	33.0	40.0
31	37.26250870328145	39.0	38.0	40.0	33.0	40.0
32	37.180331104884395	39.0	37.0	40.0	33.0	40.0
33	37.10669462554651	39.0	37.0	40.0	33.0	40.0
34	37.037692810613514	39.0	37.0	40.0	32.0	40.0
35	36.95897359274094	39.0	37.0	40.0	32.0	40.0
36	36.911550964842206	39.0	37.0	40.0	32.0	40.0
37	37.035800780591394	39.0	37.0	40.0	33.0	40.0
38	36.953290482657685	39.0	37.0	40.0	32.0	40.0
39	36.87856055563852	39.0	37.0	40.0	32.0	40.0
40	36.94738935794507	39.0	37.0	40.0	32.0	40.0
41	37.14019045133364	39.0	38.0	40.0	33.0	40.0
42	37.07200357414922	39.0	38.0	40.0	33.0	40.0
43	37.0104534209195	39.0	37.0	40.0	33.0	40.0
44	36.92339760047941	39.0	37.0	40.0	32.0	40.0
45	36.854196441698775	39.0	37.0	40.0	32.0	40.0
46	36.77000577340967	39.0	37.0	40.0	32.0	40.0
47	36.68032665887371	39.0	37.0	40.0	32.0	40.0
48	36.560926960468414	39.0	36.0	40.0	32.0	40.0
49	36.468026865136586	39.0	36.0	40.0	31.0	40.0
50	36.352506378793755	39.0	36.0	40.0	31.0	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1	1	-0.29719408117318125
1	2	-0.29941359247815313
1	3	-0.31382969493412105
1	4	-0.29282016248841813
1	5	-0.32804259983129924
1	6	-0.33084175336898625
1	7	-0.3879097164252059
1	8	-0.3604054023834564
1	9	-0.3474653086920583
1	10	-0.39246286809768804
1	11	-0.3238147147401591
1	12	-0.3848023892691401
1	13	-0.4000941462742418
1	14	-0.4191301362087714
1	15	-0.39836805211074733
1	16	-0.39167838292419077
1	17	-0.38601529203150164
1	18	-0.417866042155687
1	19	-0.40761721390057204
1	20	-0.6382063849680435
1	21	-0.4183465526653336
1	22	-0.4226003420946398
1	23	-0.41769109971753693
1	24	-0.41374684588982547
1	25	-0.4384878243016672
1	26	-0.4531333717574384
1	27	-0.4549652467309073
1	28	-0.406024940353781
1	29	-0.460090841704897
1	30	-0.3842224627715467
1	31	-0.49952967951034566
1	32	-0.5637579503963579
1	33	-0.5763558410070431
1	34	-0.556404077135376
1	35	-0.5632866760670012
1	36	-0.4393128046194761
1	37	-0.6154821196146827
1	38	-0.6824213893436735
1	39	-0.6325734977293038
1	40	-0.411148043351254
1	41	-0.36824739284214303
1	42	-0.3781946713380293
1	43	-0.36621298233689004
1	44	-0.42774524273789183
1	45	-0.42359133290722895
1	46	-0.43434354753544113
1	47	-0.5579689573479882
1	48	-0.4356077386159072
1	49	-0.35997791300856363
1	50	-0.4047739345788912
2	1	-0.1824121139314414
2	2	-0.2207327932660803
2	3	-0.199386188499318
2	4	-0.21408956084937358
2	5	-0.24289146173055087
2	6	-0.23420232259748985
2	7	-0.23419260099297645
2	8	-0.22489931376811967
2	9	-0.2292102897654189
2	10	-0.22410354954455158
2	11	-0.2495273073960078
2	12	-0.26074119600387746
2	13	-0.26503313691116404
2	14	-0.286651358392227
2	15	-0.2937337724401985
2	16	-0.26385930766714694
2	17	-0.29263125244256827
2	18	-0.2584961246990929
2	19	-0.2794881008364456
2	20	-0.31538339632393075
2	21	-0.32600487121560207
2	22	-0.29706440853904326
2	23	-0.32745417298936275
2	24	-0.30784499772904184
2	25	-0.2670086179964599
2	26	-0.3129657626226461
2	27	-0.244079075071177
2	28	-0.2605104997592207
2	29	-0.23426186233966462
2	30	-0.28591974377823703
2	31	-0.28493219166293926
2	32	-0.27754692406189463
2	33	-0.3069839760642523
2	34	-0.3232230667009546
2	35	-0.3737174585700984
2	36	-0.333492438950195
2	37	-0.41891932941503285
2	38	-0.4385609619000377
2	39	-0.41349756024665396
2	40	-0.38588257692423866
2	41	-0.3737517367678507
2	42	-0.39564834680371064
2	43	-0.42029866945769356
2	44	-0.38297590468270926
2	45	-0.43153433451505663
2	46	-0.3763611429743037
2	47	-0.4029427608414409
2	48	-0.426974838240028
2	49	-0.4963346113226663
2	50	-0.483538500316115
3	1	-0.14421294664457918
3	2	-0.1578672092462341
3	3	-0.15508011949182077
3	4	-0.14695402657235235
3	5	-0.16116648312728898
3	6	-0.18455037370139848
3	7	-0.21829793472134185
3	8	-0.18296621054581408
3	9	-0.2018969523090277
3	10	-0.19664383762259519
3	11	-0.2052643097483866
3	12	-0.19485245763584658
3	13	-0.20657126018538463
3	14	-0.18818527698603305
3	15	-0.18345513889798326
3	16	-0.15263685904511703
3	17	-0.16675423648872112
3	18	-0.19436177029745494
3	19	-0.16792052746612285
3	20	-0.18123944793686775
3	21	-0.11001498286596245
3	22	-0.11960901399726254
3	23	-0.14774982132706782
3	24	-0.11977777268648993
3	25	-0.11631261447435293
3	26	-0.04608212658433075
3	27	-0.08832467712623782
3	28	-0.12929126177593986
3	29	-0.108326869044987
3	30	-0.08159466037744068
3	31	-0.11366618984892085
3	32	-0.10417525267667571
3	33	-0.1172120896269675
3	34	-0.13571390421109442
3	35	-0.13532994131305287
3	36	-0.13301079505792046
3	37	-0.2128630166514469
3	38	-0.19205216138661996
3	39	-0.18767968318831407
3	40	-0.1400109336798181
3	41	-0.12704607363465215
3	42	-0.16046102673669793
3	43	-0.1576464306878762
3	44	-0.1604142550759704
3	45	-0.19525386638027697
3	46	-0.12266972145621935
3	47	-0.11592725827021866
3	48	-0.12347451794037312
3	49	-0.1010227651090716
3	50	-0.12322903346614567
4	1	-0.13517769684210634
4	2	-0.1413262524813561
4	3	-0.1044656176994394
4	4	-0.11909356700319051
4	5	-0.12810329850786673
4	6	-0.12487654621430266
4	7	-0.1341250962811671
4	8	-0.15896199598272176
4	9	-0.14811061919478874
4	10	-0.15534905499498564
4	11	-0.1677653921710558
4	12	-0.15305325570494688
4	13	-0.17301726976958776
4	14	-0.15905294699958716
4	15	-0.16472727893879124
4	16	-0.12666047657736357
4	17	-0.12891017598487053
4	18	-0.15432356977149198
4	19	-0.12398884328592175
4	20	-0.14968679604474033
4	21	-0.09653479072528626
4	22	-0.15170450240994882
4	23	-0.1381160403773265
4	24	-0.15584044047233903
4	25	-0.07443147097615821
4	26	-0.06794817281913623
4	27	-0.06550390308009924
4	28	-0.08626105476048451
4	29	-0.08957733168504944
4	30	-0.07561930264450467
4	31	-0.09012729504237171
4	32	-0.08159060598315904
4	33	-0.11848001907459604
4	34	-0.058091701689583886
4	35	-0.10708311371092094
4	36	-0.09803156309956051
4	37	-0.18217778334479817
4	38	-0.18809426582967603
4	39	-0.21023156566090506
4	40	-0.1304513736359354
4	41	-0.04040933104415245
4	42	-0.07874337103552165
4	43	-0.10606978643717468
4	44	-0.0878353316794076
4	45	-0.07130643403572634
4	46	-0.044822709980763875
4	47	-0.04921087252893841
4	48	-0.031198276727224084
4	49	-0.05663107920691601
4	50	-0.04526115456052793
5	1	-0.07251813770924542
5	2	-0.10186620146950531
5	3	-0.11826102898618274
5	4	-0.111259835047818
5	5	-0.0826522966574359
5	6	-0.09777245438120019
5	7	-0.1057392360371665
5	8	-0.10272231087941464
5	9	-0.09561209747944588
5	10	-0.08638052995534196
5	11	-0.10645938283359158
5	12	-0.11465595162546549
5	13	-0.10548201893189457
5	14	-0.1361766022018287
5	15	-0.13018131090504426
5	16	-0.12331822940817005
5	17	-0.10891288249608522
5	18	-0.11895329256002185
5	19	-0.0878520644461318
5	20	-0.05604297880485376
5	21	-0.0530135019552489
5	22	-0.05226139651180972
5	23	-0.01084911659209098
5	24	-0.04594402336365988
5	25	-0.052857928888442984
5	26	0.023625457524381943
5	27	0.02893083322627632
5	28	0.010255635882344905
5	29	-0.0043834189818028335
5	30	0.004668323009695996
5	31	0.0011721539472446807
5	32	-0.004843803049539019
5	33	0.04720303669424197
5	34	0.005836534577078112
5	35	0.011921007834970965
5	36	0.0187916105201964
5	37	-0.017894611666676496
5	38	-0.008880713268929696
5	39	0.00973895690168547
5	40	0.061800649628288795
5	41	0.07721549943116202
5	42	0.08612170055464219
5	43	0.056690825587381255
5	44	0.11141302308537604
5	45	0.059498370610057805
5	46	0.09552754787326734
5	47	0.13733703124870544
5	48	0.17209763614665263
5	49	0.12715191743053822
5	50	0.03605244477579106
6	1	-0.11792889276445351
6	2	-0.0867792980928428
6	3	-0.0626465884838936
6	4	-0.09464437668662384
6	5	-0.07091041427726452
6	6	-0.08354335722341233
6	7	-0.1097474878599698
6	8	-0.08210655186243088
6	9	-0.10554757006579507
6	10	-0.09486869073366222
6	11	-0.1081127038741414
6	12	-0.08788210573644761
6	13	-0.09172837614376306
6	14	-0.10268710192851671
6	15	-0.12261513793544765
6	16	-0.07126430099282999
6	17	-0.07793483949354396
6	18	-0.07215653618791151
6	19	-0.09401818323264166
6	20	-0.0745516132624715
6	21	-0.05498245427423143
6	22	-0.04513959716232563
6	23	-0.07650724391228891
6	24	-0.057463720338084556
6	25	-0.022231324097674587
6	26	-0.05696702296962286
6	27	-0.0659029646754945
6	28	-0.07705082295959897
6	29	-0.050234305958078096
6	30	-0.03190458351986081
6	31	-0.019981196363197284
6	32	-0.08108914728104821
6	33	-0.0529232781682083
6	34	-0.05197906476047365
6	35	-0.08671880602087612
6	36	-0.013510912662589192
6	37	-0.0923537800128571
6	38	-0.09037606338236515
6	39	-0.04750381365417411
6	40	-0.008402231068565413
6	41	-0.0156186038392363
6	42	-0.08026220372973114
6	43	-0.06459580212280258
6	44	-0.05546287545653428
6	45	-0.04901771039211411
6	46	-0.0464568965264931
6	47	-0.034826964208278355
6	48	-0.045286947784155984
6	49	-0.0014030536254594494
6	50	0.018835677653626703
7	1	-0.08646734382254095
7	2	-0.09106899317953321
7	3	-0.07166225938929216
7	4	-0.11067986977074185
7	5	-0.0941473148505807
7	6	-0.08212797759254187
7	7	-0.1074228197939533
7	8	-0.09453208499778754
7	9	-0.10994075409234938
7	10	-0.13741660555391633
7	11	-0.10229882639782772
7	12	-0.10459813906113169
7	13	-0.10458778107047806
7	14	-0.09776584132003308
7	15	-0.11576431302572843
7	16	-0.05950723297344496
7	17	-0.04802859744507515
7	18	-0.059811894643303276
7	19	-0.07132060379423422
7	20	-0.009834899519937323
7	21	-0.04825778640064726
7	22	-0.03363149121494757
7	23	-0.04352256317601899
7	24	-0.022759783248943677
7	25	-0.040178970607925635
7	26	-0.0065289763885942875
7	27	-0.016732008856372715
7	28	-0.002292838722091517
7	29	-0.02202651989113491
7	30	-0.020932688662419707
7	31	0.011926291005003975
7	32	-0.047902723247126744
7	33	0.012414076320276024
7	34	0.022095096568421013
7	35	-0.02057280658648608
7	36	0.038881812045126196
7	37	-0.045451686903064115
7	38	-0.05012631403599954
7	39	-0.05485516549578762
7	40	0.041463901162764216
7	41	0.017656770593973192
7	42	-0.022414046256635345
7	43	0.001336999341901901
7	44	0.011356382921121622
7	45	0.0070793594601852305
7	46	0.07981431702593511
7	47	0.06565431525233834
7	48	0.08637978135224955
7	49	0.07867113014443561
7	50	0.14707713936684286
8	1	-0.08535035856689888
8	2	-0.08627399825848414
8	3	-0.0642616388846804
8	4	-0.10551632999585792
8	5	-0.07097897700262479
8	6	-0.06348580918671587
8	7	-0.1165522087290114
8	8	-0.09043086117493004
8	9	-0.0923254387354362
8	10	-0.07145634324385952
8	11	-0.07774458162299425
8	12	-0.08353577180653815
8	13	-0.09303729549162654
8	14	-0.07604215184424135
8	15	-0.07252515838951723
8	16	-0.06323324260806373
8	17	-0.05953092273416871
8	18	-0.06428073346088325
8	19	-0.04803160870901735
8	20	-0.012339330779624902
8	21	-0.019708930961037652
8	22	-0.019498786851151806
8	23	-0.01427416509883983
8	24	0.01434541139301615
8	25	0.024236603313951832
8	26	0.049037993989301754
8	27	0.05832227567795201
8	28	0.02583729153514014
8	29	0.01744167123146667
8	30	0.06230108561652514
8	31	0.019793412212457895
8	32	0.04008160509959424
8	33	0.022040042091198586
8	34	0.02473567202937943
8	35	-0.021416188811173242
8	36	0.026913499078915493
8	37	-0.006837523288901082
8	38	-0.06473872951501392
8	39	-0.024532235041554884
8	40	-0.028977672902755103
8	41	0.004174230141899216
8	42	-0.050312288887020884
8	43	-0.01856560913738292
8	44	-0.04451166942801876
8	45	-0.03808601438165482
8	46	0.03794542203203832
8	47	0.013776352528786617
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114	15	-0.3230506324285187
114	16	-0.30676058646564286
114	17	-0.4464244686364722
114	18	-0.3863966431914747
114	19	-0.3308602859041301
114	20	-0.47619997284616034
114	21	-0.401062066505645
114	22	-0.28680666003185706
114	23	-0.3111705460398184
114	24	-0.390406095045833
114	25	-0.289970794205729
114	26	-0.3561822988429455
114	27	-0.29924769621554503
114	28	-0.3250334122055989
114	29	-0.2913117265556764
114	30	-0.30721647966229426
114	31	-0.21619927374950976
114	32	-0.23476793626478099
114	33	-0.2744493847723888
114	34	-0.20146154251449389
114	35	-0.2603851154686794
114	36	-0.25750154533182723
114	37	-0.22961637930324486
114	38	-0.19024467947876644
114	39	-0.2080103936912252
114	40	-0.14365371240916147
114	41	-0.14652480176737015
114	42	-0.16088958683785393
114	43	-0.10195525961836438
114	44	-0.13679985370565362
114	45	-0.10246969599473488
114	46	0.048111368065598015
114	47	-0.008015019956673086
114	48	0.031951132423763795
114	49	0.07356018437716472
114	50	0.11047035568163466
115	1	-0.36020798921381214
115	2	-0.417247326321629
115	3	-0.36052919144468376
115	4	-0.4166306838752263
115	5	-0.4301376532605232
115	6	-0.3332332086354981
115	7	-0.42172588488529783
115	8	-0.4589045316194955
115	9	-0.3780932967668349
115	10	-0.38108548954868837
115	11	-0.38423247612524136
115	12	-0.36200384968039856
115	13	-0.4005439879170609
115	14	-0.37451455248442045
115	15	-0.37483337719078236
115	16	-0.3961074322226139
115	17	-0.4384678602211878
115	18	-0.3933240201684356
115	19	-0.46801943219449527
115	20	-0.3189925444111523
115	21	-0.3095907464393761
115	22	-0.3825293393639626
115	23	-0.460373174011913
115	24	-0.31138980564264784
115	25	-0.4091620394597797
115	26	-0.48362935562976617
115	27	-0.37361766232604054
115	28	-0.4351281293420186
115	29	-0.43772074648894943
115	30	-0.4421255755802136
115	31	-0.4071202882775822
115	32	-0.28803193643785363
115	33	-0.2954088296298494
115	34	-0.3405163322379394
115	35	-0.28390158759370365
115	36	-0.20731983604151338
115	37	-0.3190106446558616
115	38	-0.2932070526770616
115	39	-0.3240722926760995
115	40	-0.22692749143622137
115	41	-0.1625724610664392
115	42	-0.16337435290186164
115	43	-0.1708036747865478
115	44	-0.26472194010003136
115	45	-0.15914267677096205
115	46	-0.1917339517016572
115	47	-0.20022470679497673
115	48	-0.17561342802687108
115	49	-0.18084608733465757
115	50	-0.12955329451423836
116	1	-0.3363610525780274
116	2	-0.4584921203021608
116	3	-0.4164046228268887
116	4	-0.5018163148997417
116	5	-0.37177318857555974
116	6	-0.41235987919167627
116	7	-0.3987594023990795
116	8	-0.44314731099805726
116	9	-0.42931713307830677
116	10	-0.5234034737711823
116	11	-0.4749687840450818
116	12	-0.42939020009254847
116	13	-0.47857637345465776
116	14	-0.41641623722487253
116	15	-0.44703124995022847
116	16	-0.3556815407544107
116	17	-0.35477843140977683
116	18	-0.40189269629090063
116	19	-0.42601171632006896
116	20	-0.4904743016683568
116	21	-0.46424865796369374
116	22	-0.41865391710636857
116	23	-0.405623498913009
116	24	-0.5049518809049829
116	25	-0.4303591610152395
116	26	-0.4630333043881478
116	27	-0.4111408175899314
116	28	-0.5918576463285206
116	29	-0.5048194444457508
116	30	-0.38841573508639726
116	31	-0.3814125062088962
116	32	-0.35098202081874774
116	33	-0.32152148947458414
116	34	-0.35304910633733755
116	35	-0.44939498017172497
116	36	-0.3180763390013084
116	37	-0.43244635320976954
116	38	-0.3378339519683351
116	39	-0.3633087068192822
116	40	-0.3693486132600725
116	41	-0.5957927937062308
116	42	-0.4302286708708323
116	43	-0.349077588647674
116	44	-0.46792343265389036
116	45	-0.48758150604897565
116	46	-0.28652855534615895
116	47	-0.2414032795547385
116	48	-0.252018821292765
116	49	-0.20587450087145243
116	50	-0.13578596080854055
117	1	-0.45150183506349606
117	2	-0.4781157353187666
117	3	-0.5210256362499237
117	4	-0.5392306720200608
117	5	-0.4961504399773844
117	6	-0.48223909755747485
117	7	-0.5305546896180289
117	8	-0.5288850506535354
117	9	-0.5295770815431027
117	10	-0.5520138674712172
117	11	-0.45078254457629185
117	12	-0.4797825080894995
117	13	-0.4597435857569465
117	14	-0.4995650316536029
117	15	-0.4819480047278262
117	16	-0.41776623812747005
117	17	-0.45463800470284355
117	18	-0.5725028205227289
117	19	-0.416851886304066
117	20	-0.3921451051731708
117	21	-0.5032357234602785
117	22	-0.5332719337907861
117	23	-0.4694224376274079
117	24	-0.8953585997087004
117	25	-0.5355578180430101
117	26	-0.6107322304697362
117	27	-0.4944111072818629
117	28	-0.5120375863564561
117	29	-0.455839228722013
117	30	-0.8023590911767258
117	31	-0.5118362767794551
117	32	-0.5991286921198125
117	33	-0.4472046419873692
117	34	-0.5611177026003844
117	35	-0.46241377898287084
117	36	-0.46143694538409363
117	37	-0.5306458496321795
117	38	-0.656752103576288
117	39	-0.6491366697811358
117	40	-0.42393051324296493
117	41	-0.4598728159657739
117	42	-0.5693676577994395
117	43	-0.3958606417615016
117	44	-0.33961411432261457
117	45	-0.40781259886153265
117	46	-0.26871074432659725
117	47	-0.24493018369361153
117	48	-0.5142630240708996
117	49	-0.3840170716916589
117	50	-0.33043968972577176
118	1	-0.36818735837852046
118	2	-0.411380764160306
118	3	-0.46814616280237686
118	4	-0.417609981967928
118	5	-0.45619925581811316
118	6	-0.4280366121050818
118	7	-0.43329096833662817
118	8	-0.4954998917380138
118	9	-0.48992583465038564
118	10	-0.4735161135916215
118	11	-0.46554772605433925
118	12	-0.5511874839903967
118	13	-0.48097119330406457
118	14	-0.4745747373573508
118	15	-0.4659593953093122
118	16	-0.4291454484482742
118	17	-0.5148842063248722
118	18	-0.5366270483851849
118	19	-0.6221888840262224
118	20	-0.514942421512508
118	21	-0.5628731712446609
118	22	-0.5248403144043507
118	23	-0.7077383567894131
118	24	-0.5920847983722695
118	25	-0.523598537616806
118	26	-0.6294492877205471
118	27	-0.5290197961736851
118	28	-0.5793986640047706
118	29	-0.6881445532768282
118	30	-0.6354766662040348
118	31	-0.6756682309957682
118	32	-0.646437838117258
118	33	-0.6132322536946333
118	34	-0.6888497447362454
118	35	-0.6153138975605401
118	36	-0.618343347430816
118	37	-0.6455348680005031
118	38	-0.5920856598117723
118	39	-0.692172461515824
118	40	-0.8102567880077842
118	41	-0.5921627742875231
118	42	-0.7512105885430884
118	43	-0.6354199988209928
118	44	-0.7279697565836898
118	45	-0.6713126133633764
118	46	-0.7075212756628204
118	47	-0.613674512755729
118	48	-0.6034283888665399
118	49	-0.7563425635573324
118	50	-1.0697812356153236
120	1	-0.3980510866998799
120	2	-0.4097804973446415
120	3	-0.41133116441771733
120	4	-0.41892783640901143
120	5	-0.4345001102573036
120	6	-0.848582526685675
120	7	-0.9099618083990819
120	8	-0.9649877841086365
120	9	-1.0550296455496664
120	10	-1.3461136254058417
120	11	-1.7583181402006929
120	12	-2.270631623070315
120	13	-1.8158024218040438
120	14	-1.7576597841038009
120	15	-1.6313663162881653
120	16	-3.895207739665267
120	17	-1.5670291379142611
120	18	-1.802054596149837
120	19	-1.7630620420898069
120	20	-1.9570329750789952
120	21	-2.0407207462849613
120	22	-2.5581939436753274
120	23	-1.9491303987988715
120	24	-1.7453097119545404
120	25	-2.7851385587664694
120	26	-3.1392576999161648
120	27	-4.822558517221395
120	28	-1.7672853822479695
120	29	-1.888413590373105
120	30	-2.2031480111649344
120	31	-1.8527287700654043
120	32	-2.0947729739286842
120	33	-1.7575624467286346
120	34	-1.6924677687008582
120	35	-1.744683624116412
120	36	-1.2107013554609196
120	37	-1.4030489829841954
120	38	-1.4960504289345025
120	39	-1.6687580717055184
120	40	-1.64021449552272
120	41	-1.5549288472959688
120	42	-1.7230346356450639
120	43	-1.5015148433243368
120	44	-2.041528572045074
120	45	-1.7922982764476032
120	46	-2.3820396960824937
120	47	-2.3508359061116977
120	48	-2.8224416646853356
120	49	-2.721620513246606
120	50	-2.3858009178126167
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	57145.0
3	2136.0
4	3670.0
5	3789.0
6	5542.0
7	9387.0
8	10393.0
9	11910.0
10	16208.0
11	19682.0
12	23018.0
13	28966.0
14	33168.0
15	37123.0
16	39975.0
17	44231.0
18	50225.0
19	58165.0
20	68555.0
21	68586.0
22	84440.0
23	108567.0
24	134948.0
25	158034.0
26	200237.0
27	247125.0
28	298127.0
29	351901.0
30	423786.0
31	509824.0
32	617201.0
33	773005.0
34	1024513.0
35	1451435.0
36	2199086.0
37	3696918.0
38	5888517.0
39	2.1647963E7
40	190692.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	15.711008388501863	29.786534236892226	28.42513238795427	26.07732498665164
2	23.417645706546594	31.017700713920938	25.499359047827575	20.065294531704897
3	23.53308350395897	28.04269522054733	26.503649829972215	21.92057144552148
4	24.292895841056406	26.52070727534943	25.68587900157766	23.5005178820165
5	24.800738479200554	28.90050154897914	26.90760430758843	19.391155664231878
6	25.470812766274832	26.90179380843809	24.020067248480544	23.607326176806538
7	23.43672994444023	27.096344326725735	27.813704809038537	21.653220919795498
8	24.43026115320196	27.034561318317806	26.19420061430367	22.340976914176558
9	23.582116080880745	26.286480287435452	26.90985039654351	23.221553235140295
10	22.449107525549227	28.134257108438298	26.35657454015256	23.06006082585991
11	23.423988353373264	27.646161492951176	26.04528236022722	22.884567793448344
12	22.805352445119908	26.590917482460362	27.347872354811457	23.25585771760827
13	22.891284841175075	27.121736674339175	26.48577733496661	23.501201149519144
14	22.85337400054845	28.12799236300007	26.514137679358274	22.504495957093205
15	22.186466347502666	27.782872153817074	27.13815305753125	22.892508441149012
16	22.511107332323505	26.67713691362866	27.10304864652031	23.70870710752752
17	23.3015863538557	26.595127029422223	26.7164353842054	23.38685123251668
18	22.205471066162968	27.029173441290848	26.679788432948236	24.08556705959795
19	23.016019456826562	27.58039994538673	26.684064485333124	22.71951611245358
20	23.480756889844827	26.756232721983466	26.722898726059064	23.040111662112647
21	22.620221052695623	27.899936334604842	26.638328459594245	22.841514153105287
22	23.73246196443733	26.745216966569203	26.6779627367624	22.844358332231067
23	22.779269565868525	26.68991975872813	27.798038262055314	22.732772413348034
24	22.362714878088255	27.90481377663444	26.496508273405304	23.235963071872
25	22.54082318409394	27.018825159254156	26.558475824436517	23.88187583221538
26	22.7347681787331	26.97234904326503	27.607729941166227	22.685152836835645
27	23.007453859944484	26.734154632794972	27.420303674899245	22.838087832361296
28	22.982181129784333	26.838244743586937	26.931365376724443	23.248208749904293
29	23.26458950224238	26.69416902900737	26.98209786105716	23.05914360769309
30	23.335776757926457	26.822439666312377	26.97676031172736	22.86502326403381
31	22.88637034802415	27.17542892755402	27.03038085523235	22.907819869189478
32	23.124114992680692	26.77699110460506	27.019094329663606	23.079799573050643
33	22.86557667617732	27.161387892189964	26.904202948739353	23.068832482893363
34	22.894425744472173	26.804233719427323	27.284132952894097	23.01720758320641
35	22.717459264070385	26.910009115123597	27.08784196236058	23.284689658445444
36	23.003333081096258	26.830681275896023	27.109507657236847	23.05647798577087
37	23.008863158455124	26.918012953473138	26.876079858000796	23.19704403007095
38	22.72405004051977	27.45556629799426	26.94957868372509	22.87080497776088
39	22.872054293832008	27.153364425545085	26.90290961943115	23.071671661191758
40	22.9297648172569	26.864770884894106	26.965508366549827	23.239955931299168
41	22.71616397840701	27.46781935469481	26.874391347270294	22.94162531962788
42	23.24557877267988	26.829555872246182	26.86059237598169	23.064272979092248
43	22.862840356463995	26.864479498383076	26.90662075094423	23.366059394208694
44	23.025479779049338	27.14734463993037	26.861014414672173	22.96616116634812
45	22.92508052036858	27.0333452525866	26.903815624726718	23.1377586023181
46	22.67020131256973	26.936177575826843	27.315253491531887	23.078367620071536
47	22.95829777418755	26.968932489017767	27.074857025327	22.997912711467684
48	22.597229628967902	26.89170450790514	26.73226911456561	23.77879674856135
49	22.643430485027118	27.274705934788035	26.716752407529825	23.365111172655023
50	23.222213118192073	26.976017606398838	26.75660663956343	23.04516263584566
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	94909.0
1	51684.0
2	8459.0
3	6425.5
4	4392.0
5	4261.0
6	4130.0
7	4827.5
8	5525.0
9	7477.0
10	9429.0
11	13805.5
12	18182.0
13	25833.5
14	33485.0
15	49335.5
16	65186.0
17	95316.0
18	125446.0
19	174906.0
20	224366.0
21	298127.0
22	371888.0
23	556294.5
24	740701.0
25	724925.5
26	709150.0
27	816176.5
28	923203.0
29	1024242.5
30	1125282.0
31	1257697.5
32	1390113.0
33	1515271.5
34	1640430.0
35	1775189.0
36	1909948.0
37	2026483.5
38	2143019.0
39	2237558.5
40	2332098.0
41	2389576.5
42	2447055.0
43	2499787.0
44	2552519.0
45	2565781.0
46	2579043.0
47	2529596.0
48	2480149.0
49	2452355.0
50	2424561.0
51	2380180.0
52	2335799.0
53	2272930.0
54	2210061.0
55	2111025.5
56	2011990.0
57	1981249.5
58	1950509.0
59	1808242.0
60	1665975.0
61	1458298.5
62	1250622.0
63	1095359.0
64	940096.0
65	811686.0
66	683276.0
67	583808.0
68	484340.0
69	404570.0
70	324800.0
71	251319.5
72	177839.0
73	142609.5
74	107380.0
75	79103.0
76	50826.0
77	37641.0
78	24456.0
79	17014.5
80	9573.0
81	6840.5
82	4108.0
83	3118.5
84	2129.0
85	1606.5
86	1084.0
87	742.0
88	400.0
89	260.5
90	121.0
91	86.0
92	51.0
93	38.0
94	25.0
95	26.0
96	27.0
97	22.0
98	17.0
99	19.0
100	21.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.06812864799179609
2	0.0
3	0.02058466001183846
4	3.5223242571411984E-4
5	4.0395886585395564E-4
6	2.487795454344483E-4
7	0.0011650764850543963
8	7.635807830166234E-5
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	1.2808451844149814E-4
15	1.1576869936058485E-4
16	0.007788523986769559
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	7.389491448547968E-6
23	1.970531052946125E-5
24	0.00902256905867707
25	0.002413900539859003
26	0.024486311496671784
27	0.05460587864095331
28	0.04574587839414429
29	0.04354627310629318
30	0.03893523044239924
31	0.093223360951065
32	0.13484097678928716
33	0.11534996151183378
34	0.11877868554396004
35	0.14255806902538742
36	0.1338113743141228
37	0.15318908405603174
38	0.16591625149424755
39	0.15059537255759142
40	0.16039383821836603
41	0.16521917613426784
42	0.1845968858761768
43	0.21969697025677964
44	0.20465196566753602
45	0.2066840758158867
46	0.1962156295971104
47	0.2173027750274501
48	0.18676447003441754
49	0.21854667275462233
50	0.22439914998187235
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4.0598193E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.24558407650831
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.4590493053564	35.82138977137262
2	11.50923887173668	10.645029477990326
3	3.986715943033871	5.53104021998187
4	1.9560400463945686	3.6183285769022895
5	1.1148276061281654	2.5777926895006287
6	0.7397628758407545	2.0526459762823914
7	0.5015551368766229	1.6236297176002747
8	0.36743468615848274	1.3593785337094049
9	0.2675128383918011	1.1134158713454076
>10	1.7780636242362515	16.06928336524234
>50	0.20379653441453446	6.50058792738602
>100	0.10991356920595338	9.602537720741095
>500	0.005249033615841802	1.5836287150312718
>1k	7.86657685456009E-4	0.5408360276456039
>5k	1.598127994861072E-5	0.050335157859857156
>10k+	3.728964464384754E-5	1.3101402514086546
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	189763	0.4674173552502694	No Hit
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAG	133620	0.32912794911832655	Illumina Paired End PCR Primer 2 (97% over 36bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	87396	0.21527066487909943	No Hit
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCG	79052	0.19471802599687135	Illumina Paired End PCR Primer 2 (100% over 31bp)
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.857270026771881E-4	0.0	0.0	4.926327632365313E-6	0.0
2	2.980428217581014E-4	0.0	0.0	7.389491448547969E-6	0.0
3	3.054323132066494E-4	0.0	0.0	9.852655264730625E-6	0.0
4	3.054323132066494E-4	0.0	0.0	1.2315819080913282E-5	0.0
5	3.054323132066494E-4	0.0	0.0	1.4778982897095938E-5	0.0
6	3.07895477022832E-4	2.4631638161826563E-6	0.0	1.970531052946125E-5	0.0
7	3.1282180465519736E-4	9.852655264730625E-6	0.0	2.2168474345643905E-5	0.0
8	3.2267445991992794E-4	9.852655264730625E-6	0.0	2.2168474345643905E-5	0.0
9	3.546955895303025E-4	9.852655264730625E-6	0.0	2.4631638161826563E-5	0.0
10	6.330331007589426E-4	9.852655264730625E-6	0.0	2.7094801978009218E-5	0.0
11	7.192438343253357E-4	9.852655264730625E-6	0.0	2.7094801978009218E-5	0.0
12	9.236864310684961E-4	1.2315819080913282E-5	0.0	2.7094801978009218E-5	0.0
13	0.0011724659765029444	1.2315819080913282E-5	0.0	2.7094801978009218E-5	0.0
14	0.001216802925194232	1.2315819080913282E-5	0.0	2.7094801978009218E-5	0.0
15	0.01030341424309205	1.2315819080913282E-5	0.0	2.7094801978009218E-5	2.4631638161826563E-6
16	0.010532488477997037	1.2315819080913282E-5	0.0	2.7094801978009218E-5	2.4631638161826563E-6
17	0.011249269148506191	1.2315819080913282E-5	0.0	2.9557965794191876E-5	2.4631638161826563E-6
18	0.011830575809125297	1.7242146713278593E-5	0.0	2.9557965794191876E-5	2.4631638161826563E-6
19	0.013062157717216626	1.7242146713278593E-5	0.0	3.2021129610374534E-5	2.4631638161826563E-6
20	0.21834469332169537	1.7242146713278593E-5	0.0	3.2021129610374534E-5	2.4631638161826563E-6
21	0.22260842988750756	1.7242146713278593E-5	0.0	3.4484293426557185E-5	2.4631638161826563E-6
22	0.22325870513497978	1.7242146713278593E-5	0.0	3.4484293426557185E-5	2.4631638161826563E-6
23	0.2238942013995549	1.970531052946125E-5	0.0	3.4484293426557185E-5	2.4631638161826563E-6
24	0.2250740568675064	1.970531052946125E-5	0.0	3.94106210589225E-5	2.4631638161826563E-6
25	0.22570955313208152	2.2168474345643905E-5	0.0	3.94106210589225E-5	2.4631638161826563E-6
26	0.22638692318153175	2.7094801978009218E-5	0.0	5.172644013983578E-5	2.4631638161826563E-6
27	0.2272046935685044	2.7094801978009218E-5	0.0	5.172644013983578E-5	2.4631638161826563E-6
28	0.2633491594071687	2.7094801978009218E-5	0.0	5.4189603956018436E-5	2.4631638161826563E-6
29	0.26552905938449034	3.2021129610374534E-5	0.0	5.6652767772201094E-5	2.4631638161826563E-6
30	0.27395800596346737	3.4484293426557185E-5	0.0	5.911593158838375E-5	2.4631638161826563E-6
31	0.6184043708546338	3.4484293426557185E-5	0.0	6.157909540456641E-5	2.4631638161826563E-6
32	0.6219045266374295	3.694745724273984E-5	0.0	6.157909540456641E-5	2.4631638161826563E-6
33	0.6369372154075922	3.694745724273984E-5	0.0	6.157909540456641E-5	2.4631638161826563E-6
34	0.63806780759922	3.694745724273984E-5	0.0	6.650542303693171E-5	2.4631638161826563E-6
35	0.6394102318790396	3.694745724273984E-5	0.0	6.896858685311437E-5	2.4631638161826563E-6
36	0.6407896036161018	3.694745724273984E-5	0.0	7.143175066929703E-5	2.4631638161826563E-6
37	0.6425803237104666	3.94106210589225E-5	0.0	7.635807830166234E-5	2.4631638161826563E-6
38	0.6443365595114049	3.94106210589225E-5	0.0	7.8821242117845E-5	2.4631638161826563E-6
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	59775	0.0	31.358124	1
TCAACGC	58250	0.0	31.286901	12
ATCAACG	58805	0.0	30.991615	11
CAACGCA	59300	0.0	30.614313	13
GGTATCA	61590	0.0	29.588036	8
AACGCAG	61320	0.0	29.494707	14
GTATCAA	61885	0.0	29.468376	9
ACGCAGA	60985	0.0	29.43328	15
GTGGTAT	62240	0.0	29.184273	6
TGGTATC	62835	0.0	28.928839	7
CGCAGAG	62325	0.0	28.765127	16
AGTGGTA	64075	0.0	28.479	5
AGAGTAC	62585	0.0	27.881844	19
AGCAGTG	66855	0.0	27.856436	2
TATCAAC	66335	0.0	27.61608	10
GAGTACT	37615	0.0	27.058495	20
CAGAGTA	65210	0.0	26.927107	18
GCAGAGT	66530	0.0	26.528896	17
TATGCCG	3760	0.0	25.31513	43
CAGTGGT	72645	0.0	25.28579	4
>>END_MODULE