FastQCFastQC Report
Thu 26 May 2016
SRR522101_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR522101_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33957366
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAG1265730.37274092460528296Illumina Paired End PCR Primer 2 (97% over 36bp)
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT651550.1918729503342515No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGA231500.034.49007425
AGATCGG213050.033.05525631
TGCCGAG241650.033.01519826
AATGCCG244050.032.82164824
CGAGATC230500.031.36344129
CCGAGAT240100.030.23645828
GAGATCG244500.029.44230830
GCCGAGA276700.029.1538927
GTTCAGC320550.025.2463114
GGAATGC341550.023.9200122
GAATGCC339350.023.8696223
TATGCCG28350.023.75612843
GACCGAT30400.023.45543532
TCGTATG28550.023.43359640
CAGGAAT365100.022.42193220
CGTATGC30600.022.1522941
AGGAATG373750.021.99231121
GAGCGGT372250.021.5981569
GATCGGA391100.021.3224581
GCGGTTC383050.021.10976411
Produced by FastQC (version 0.11.5)