FastQCFastQC Report
Thu 26 May 2016
SRR522100_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR522100_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37601870
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1117310.29714213681394036No Hit
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAG693870.18453071615853148Illumina Paired End PCR Primer 2 (97% over 36bp)
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCG600850.1597925847836823Illumina Paired End PCR Primer 2 (100% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC386400.029.71315812
ATCAACG392800.029.2345911
CAACGCA397600.028.72692313
ACGCAGA408000.027.79536415
AACGCAG410500.027.78134714
AAGCAGT434800.027.3198051
GTATCAA424350.027.2412479
GGTATCA426050.027.1067268
GTGGTAT433100.026.7367346
CGCAGAG425750.026.59004416
TGGTATC439600.026.2662037
AGTGGTA447300.026.060335
TATCAAC449950.025.78514910
AGAGTAC428250.025.50453419
CAGAGTA455350.024.14967518
AGCAGTG493400.024.0781882
GCAGAGT467750.023.84447317
TCGTATG31700.023.21280140
GAGTACT263050.023.0747720
TATGCCG33000.022.23381843
Produced by FastQC (version 0.11.5)