##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR522100_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	37601870
Sequences flagged as poor quality	0
Sequence length	50
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.61918609367034	40.0	39.0	40.0	36.0	40.0
2	38.47455272836165	40.0	38.0	40.0	35.0	40.0
3	38.44636226868504	40.0	38.0	40.0	35.0	40.0
4	38.43204819334783	40.0	38.0	40.0	35.0	40.0
5	38.44202477164035	40.0	39.0	40.0	35.0	40.0
6	38.48825534474748	40.0	39.0	40.0	35.0	40.0
7	38.46078532796374	40.0	39.0	40.0	35.0	40.0
8	38.43345602226698	40.0	38.0	40.0	35.0	40.0
9	38.423215441146944	40.0	38.0	40.0	35.0	40.0
10	38.411746304106686	40.0	38.0	40.0	35.0	40.0
11	38.45512845504758	40.0	38.0	40.0	35.0	40.0
12	38.40569439764565	40.0	38.0	40.0	35.0	40.0
13	38.39085752916012	40.0	38.0	40.0	35.0	40.0
14	38.35455970673799	40.0	38.0	40.0	35.0	40.0
15	38.33678734594849	40.0	38.0	40.0	35.0	40.0
16	38.34958056607291	40.0	38.0	40.0	35.0	40.0
17	38.28027124714808	40.0	38.0	40.0	35.0	40.0
18	38.233775953164034	40.0	38.0	40.0	35.0	40.0
19	38.19221621158735	40.0	38.0	40.0	35.0	40.0
20	38.13131166082963	40.0	38.0	40.0	35.0	40.0
21	38.12392641642557	40.0	38.0	40.0	35.0	40.0
22	38.07357025062849	40.0	38.0	40.0	35.0	40.0
23	37.99239819721732	40.0	38.0	40.0	34.0	40.0
24	37.93555581145299	40.0	38.0	40.0	34.0	40.0
25	37.924164356719494	40.0	38.0	40.0	34.0	40.0
26	37.81176664883954	40.0	38.0	40.0	34.0	40.0
27	37.74592984338279	40.0	38.0	40.0	33.0	40.0
28	37.68727427651869	39.0	38.0	40.0	33.0	40.0
29	37.61724600398863	39.0	38.0	40.0	33.0	40.0
30	37.55408419847205	39.0	38.0	40.0	33.0	40.0
31	37.47174760191448	39.0	38.0	40.0	33.0	40.0
32	37.406671077794805	39.0	38.0	40.0	33.0	40.0
33	37.33188344090334	39.0	38.0	40.0	33.0	40.0
34	37.25976471382939	39.0	37.0	40.0	33.0	40.0
35	37.19212257262737	39.0	37.0	40.0	33.0	40.0
36	37.12445234771569	39.0	37.0	40.0	33.0	40.0
37	37.247024257038284	39.0	38.0	40.0	33.0	40.0
38	37.177666323509975	39.0	38.0	40.0	33.0	40.0
39	37.234525915865355	39.0	38.0	40.0	33.0	40.0
40	37.17646026647079	39.0	38.0	40.0	33.0	40.0
41	37.329955744222296	39.0	38.0	40.0	33.0	40.0
42	37.28176024224327	39.0	38.0	40.0	33.0	40.0
43	37.23241442513364	39.0	38.0	40.0	33.0	40.0
44	37.15860735117695	39.0	38.0	40.0	33.0	40.0
45	37.056933099337876	39.0	37.0	40.0	33.0	40.0
46	36.95912118200504	39.0	37.0	40.0	33.0	40.0
47	36.85471847543752	39.0	37.0	40.0	33.0	40.0
48	36.71631807141507	39.0	37.0	40.0	32.0	40.0
49	36.62918525062716	39.0	36.0	40.0	32.0	40.0
50	36.52151002064525	39.0	36.0	40.0	32.0	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1	1	-0.18158188426066602
1	2	-0.19926210212992856
1	3	-0.19990873319196112
1	4	-0.18576738399966786
1	5	-0.19660168886316853
1	6	-0.183335229396981
1	7	-0.20383563714172226
1	8	-0.20715263628790126
1	9	-0.24540078489868478
1	10	-0.22759430277355364
1	11	-0.2150044775952864
1	12	-0.2388842546893386
1	13	-0.2483927740311458
1	14	-0.23617211951997774
1	15	-0.21579876554862665
1	16	-0.2252100720721799
1	17	-0.2381612371840589
1	18	-0.2077433291569193
1	19	-0.2312653204416364
1	20	-0.25139125682378705
1	21	-0.19719406803176298
1	22	-0.2036088515890242
1	23	-0.24457799075784692
1	24	-0.23779646099735174
1	25	-0.2136072785837939
1	26	-0.14521373858360676
1	27	-0.1928630407293639
1	28	-0.1626029245378291
1	29	-0.17587337998219255
1	30	-0.14679144710604675
1	31	-0.10616491856912091
1	32	-0.13087619596293365
1	33	-0.17425080493288903
1	34	-0.19058489621427555
1	35	-0.18097907045510908
1	36	-0.186593678020472
1	37	-0.29009631540989744
1	38	-0.2315594404154524
1	39	-0.2111339415690665
1	40	-0.21663984904351707
1	41	-0.09387621737451468
1	42	-0.12056113183086836
1	43	-0.16931660031818296
1	44	-0.15016926496123517
1	45	-0.1330770464611959
1	46	-0.10186813300777686
1	47	-0.15125284796179272
1	48	-0.08572163093842988
1	49	-0.06570333619226432
1	50	-0.08707898692716043
2	1	-0.19569116381993723
2	2	-0.20438115081189778
2	3	-0.20214256331617975
2	4	-0.1999922986771523
2	5	-0.18650412871565436
2	6	-0.21996466026899242
2	7	-0.24739504383985178
2	8	-0.2510562938136829
2	9	-0.2421036071087599
2	10	-0.26683583386546417
2	11	-0.2682200428662824
2	12	-0.2818019815158266
2	13	-0.27419772806736376
2	14	-0.297443316304431
2	15	-0.25204004932860613
2	16	-0.2987434804782225
2	17	-0.24278643455114945
2	18	-0.2799709663056049
2	19	-0.29041693432019855
2	20	-0.25762406148611205
2	21	-0.22922873202794847
2	22	-0.21944222771157484
2	23	-0.23847530657673843
2	24	-0.22033041004130638
2	25	-0.22996336296038322
2	26	-0.25082199121186477
2	27	-0.21839955665974742
2	28	-0.2542165550566011
2	29	-0.2511230800732278
2	30	-0.2525017470580124
2	31	-0.24183225326504498
2	32	-0.2490444410976096
2	33	-0.27331339001872124
2	34	-0.27865163775412327
2	35	-0.35374937039983934
2	36	-0.30164747054636365
2	37	-0.4313441013923409
2	38	-0.5223943077256408
2	39	-0.352615447947791
2	40	-0.3666624419802247
2	41	-0.31736162896069686
2	42	-0.3574596818827871
2	43	-0.40199221115926065
2	44	-0.43675667050135303
2	45	-0.32593406827545834
2	46	-0.2706299250243305
2	47	-0.32760572103446606
2	48	-0.3293092633582546
2	49	-0.3669904027661275
2	50	-0.30719844182436873
3	1	-0.2675153663894818
3	2	-0.32191412018097054
3	3	-0.386760612041968
3	4	-0.45913392111810225
3	5	-0.4752747362042058
3	6	-0.43350887902056456
3	7	-0.5047229540193783
3	8	-0.512971110142928
3	9	-0.5405863120830077
3	10	-0.5293497909488849
3	11	-0.49795628106941336
3	12	-0.4608604712706992
3	13	-0.5795890301119613
3	14	-0.5503132298957709
3	15	-0.4821113884912407
3	16	-0.5874587447761925
3	17	-0.6927144905975737
3	18	-0.6424837172100339
3	19	-0.7312863790545876
3	20	-0.6657080345972375
3	21	-0.7104427360066836
3	22	-0.7264251768206194
3	23	-0.7869810794258356
3	24	-0.7933510906633643
3	25	-0.9518479252533325
3	26	-0.5432376589812904
3	27	-0.4823900509746153
3	28	-0.46155765171267404
3	29	-0.5431623202207447
3	30	-0.5771769325141136
3	31	-0.42167890134509634
3	32	-0.4748198213895307
3	33	-0.47653421327476764
3	34	-0.6110162331319984
3	35	-0.4965404784520189
3	36	-0.5028816591879419
3	37	-0.6934783434444682
3	38	-0.693362769377039
3	39	-0.6795510922828072
3	40	-0.7107453899800973
3	41	-0.7975249692005804
3	42	-0.7716559991671161
3	43	-0.9135929418901583
3	44	-0.9527250741303561
3	45	-0.8375611383154791
3	46	-1.1675399093275516
3	47	-1.0139618735304978
3	48	-0.9794775655923189
3	49	-1.054361683414342
3	50	-1.257826902767306
4	1	-0.1157664289161815
4	2	-0.14135965823627572
4	3	-0.15100251220543015
4	4	-0.18601898076497037
4	5	-0.13731094780242614
4	6	-0.13834859121857335
4	7	-0.15075943599704544
4	8	-0.19512277477987539
4	9	-0.1844096740456962
4	10	-0.1743118383883271
4	11	-0.19108057648510623
4	12	-0.1986571352832911
4	13	-0.21894383470237955
4	14	-0.1806308764924438
4	15	-0.1959867336429113
4	16	-0.2177402245861657
4	17	-0.18539771930335291
4	18	-0.21591655491921813
4	19	-0.2042037881849197
4	20	-0.2379125257928152
4	21	-0.25586160241670086
4	22	-0.2880478080171329
4	23	-0.2904252723415297
4	24	-0.23207084375641074
4	25	-0.25980980571135603
4	26	-0.3258346747836498
4	27	-0.27984221621833427
4	28	-0.2911274431562987
4	29	-0.3422507185608694
4	30	-0.2628866280671076
4	31	-0.2626119935564901
4	32	-0.32308542490587655
4	33	-0.32186071334933075
4	34	-0.27729628547034935
4	35	-0.3490890884047033
4	36	-0.31861713448510187
4	37	-0.37953955802122863
4	38	-0.39860875008022845
4	39	-0.3401858463237062
4	40	-0.3603749028148968
4	41	-0.3338308760697686
4	42	-0.5335024854683112
4	43	-0.43001760450611215
4	44	-0.369553278554271
4	45	-0.3716864293792028
4	46	-0.34688232260683094
4	47	-0.40622107837253907
4	48	-0.4660973318727386
4	49	-0.3412419719729485
4	50	-0.3908820207215129
5	1	-0.13725776721192062
5	2	-0.14236606822262132
5	3	-0.11447721941932087
5	4	-0.1432410707532341
5	5	-0.1330110661288444
5	6	-0.15368964145332598
5	7	-0.13774803319615359
5	8	-0.1339811422095778
5	9	-0.12666859993522195
5	10	-0.14705150264474298
5	11	-0.14653245744302268
5	12	-0.14389580677470093
5	13	-0.15244569849393486
5	14	-0.14797280992109307
5	15	-0.15579197741667627
5	16	-0.16327958339441295
5	17	-0.1452878368542656
5	18	-0.16690735843600635
5	19	-0.13248100281731467
5	20	-0.18397732153349722
5	21	-0.12195678925895237
5	22	-0.1719427920326595
5	23	-0.0839154974760774
5	24	-0.09557000042033792
5	25	-0.21507977176270998
5	26	-0.2309442006557063
5	27	-0.14621285719402266
5	28	-0.09371326206824904
5	29	-0.12303212800703989
5	30	-0.21172567213928772
5	31	-0.16403845973002262
5	32	-0.21853580507934112
5	33	-0.2328547723906098
5	34	-0.20294002608855521
5	35	-0.19818871407892402
5	36	-0.18437965141060886
5	37	-0.2772679447441959
5	38	-0.21021844683647117
5	39	-0.2554278917656063
5	40	-0.22512093704828828
5	41	-0.21316893768238288
5	42	-0.1857926117398776
5	43	-0.22993975834916114
5	44	-0.20549607775281942
5	45	-0.12034400994927097
5	46	-0.22688480392399413
5	47	-0.13012966362599343
5	48	-0.2242719577558816
5	49	-0.1459579327116316
5	50	-0.15062624046060336
6	1	-0.07318844076696251
6	2	-0.09798510507098257
6	3	-0.08308846245439128
6	4	-0.09299873795885816
6	5	-0.07271131319105706
6	6	-0.10306636027656424
6	7	-0.13146412069645663
6	8	-0.133359375679305
6	9	-0.1291416488850743
6	10	-0.1154885597687354
6	11	-0.10855949526487052
6	12	-0.1418964460221943
6	13	-0.1378061393423664
6	14	-0.15368597053649324
6	15	-0.12652409648509177
6	16	-0.17620021052173485
6	17	-0.151770691290821
6	18	-0.13122534719784085
6	19	-0.15617101767477948
6	20	-0.12286880731946326
6	21	-0.1304259710065736
6	22	-0.13703528448301228
6	23	-0.165571685257909
6	24	-0.10761425343523001
6	25	-0.1167535126335082
6	26	-0.1351378127273577
6	27	-0.17965549952410242
6	28	-0.18654337434730195
6	29	-0.09931154474421078
6	30	-0.16347624285439366
6	31	-0.13960363698898703
6	32	-0.09637554498174694
6	33	-0.132691579835992
6	34	-0.0893787711144256
6	35	-0.08629661064525607
6	36	-0.07205261341157865
6	37	-0.1630117922820986
6	38	-0.21025609543546864
6	39	-0.1446200917741436
6	40	-0.12145619157172405
6	41	-0.13805143824167487
6	42	-0.17194564923545386
6	43	-0.16760709767309834
6	44	-0.24444329633126927
6	45	-0.11358366310439294
6	46	-0.1698170165552071
6	47	-0.13226510230364852
6	48	-0.08902861972583764
6	49	-0.12395816832469819
6	50	-0.07233175788528712
7	1	-0.12085538644488025
7	2	-0.09168943902217563
7	3	-0.0894609699224489
7	4	-0.08350962108173832
7	5	-0.08495457866597889
7	6	-0.10795559795101894
7	7	-0.13116493643681082
7	8	-0.1270642495612222
7	9	-0.1285519351144515
7	10	-0.1530164467548829
7	11	-0.15601260538099382
7	12	-0.142285160469676
7	13	-0.1664688180420768
7	14	-0.15225151492085587
7	15	-0.17909509820067626
7	16	-0.18741702407186267
7	17	-0.12758662224180028
7	18	-0.18735463989531098
7	19	-0.1916016590992342
7	20	-0.24665050000854905
7	21	-0.2703094057814468
7	22	-0.3791244620366072
7	23	-0.29797901873060084
7	24	-0.3679600572328141
7	25	-0.3702238464416112
7	26	-0.4125757477612808
7	27	-0.3035019799365557
7	28	-0.3806063627963141
7	29	-0.44401249536417
7	30	-0.3254525632463512
7	31	-0.3172113032518311
7	32	-0.4336506689068713
7	33	-0.38345015159073625
7	34	-0.44080909170109805
7	35	-0.4718892285930707
7	36	-0.5187494117121005
7	37	-0.6301518347990225
7	38	-0.6134466986643474
7	39	-0.6890159452190403
7	40	-0.6797305458109193
7	41	-1.0104861655003532
7	42	-0.8045532930447905
7	43	-0.9285148559832663
7	44	-1.0553660742299371
7	45	-2.1032485001442964
7	46	-2.3073277324755708
7	47	-2.431892676797233
7	48	-2.7101243346306916
7	49	-2.2174789540530853
7	50	-2.7440576835855026
8	1	-0.06570349665635433
8	2	-0.08061267644358594
8	3	-0.05750326658116478
8	4	-0.046108769389348936
8	5	-0.057377233146574724
8	6	-0.06587734742996076
8	7	-0.06764809215513878
8	8	-0.07920522664855412
8	9	-0.08133189079165248
8	10	-0.07495501328147469
8	11	-0.0608545543732788
8	12	-0.08625901259132718
8	13	-0.06293197857625188
8	14	-0.04360097072789415
8	15	-0.06425989747775418
8	16	-0.10055165019517887
8	17	-0.099491702660778
8	18	-0.07035545737543458
8	19	-0.0812747484691485
8	20	-0.04677850374647363
8	21	-0.04428166342378148
8	22	-0.039362461365534784
8	23	-0.07580970830475309
8	24	-0.09142082699240461
8	25	-0.07609631862271016
8	26	-0.011968555385905688
8	27	-0.0569431914815155
8	28	-0.08921218525181729
8	29	-0.054395265094456136
8	30	-0.04973572010814564
8	31	-0.07469069893932101
8	32	-0.09409849400594084
8	33	-0.04367873259296573
8	34	-0.0442404535651022
8	35	-0.06598519736036934
8	36	-0.04271661561815421
8	37	-0.04591578845840161
8	38	-0.12257204265660704
8	39	-0.06197842806760434
8	40	-0.03766750499413973
8	41	0.004152734577935746
8	42	-0.08053727591681081
8	43	-0.0838842759373648
8	44	-0.07173797832513173
8	45	-0.09871208269097309
8	46	0.030763185209934818
8	47	-0.05265372149538905
8	48	0.016704788642549318
8	49	0.039907049608984835
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116	5	-0.20363725782754472
116	6	-0.24276357559629247
116	7	-0.2437445274732255
116	8	-0.22038262031611566
116	9	-0.2510155059371826
116	10	-0.27196952754746206
116	11	-0.27496767195139427
116	12	-0.27541853571931796
116	13	-0.29127233534680386
116	14	-0.25933504722009815
116	15	-0.34520128600856026
116	16	-0.2755379916874219
116	17	-0.3116854501915327
116	18	-0.3071385100869648
116	19	-0.36640302222711085
116	20	-0.40559617022999817
116	21	-0.38192999920264015
116	22	-0.30507470416969795
116	23	-0.42104457093091696
116	24	-0.3099024438031108
116	25	-0.38955057996513176
116	26	-0.3807851336115107
116	27	-0.31894323776444367
116	28	-0.31903230279509387
116	29	-0.46321510866489035
116	30	-0.5934103520765532
116	31	-0.3690373486522489
116	32	-0.2914378469623884
116	33	-0.3063168886979426
116	34	-0.38761381328541233
116	35	-0.4268220469175006
116	36	-0.3651793386838236
116	37	-0.4371709125681207
116	38	-0.36828194092027644
116	39	-0.47280908758477125
116	40	-0.3476501994692214
116	41	-0.4569025498950836
116	42	-0.5610790754049759
116	43	-0.37607122100745016
116	44	-0.39056904153931526
116	45	-0.5713759748377214
116	46	-0.4792203010052134
116	47	-0.38174044962011777
116	48	-0.391824269309609
116	49	-0.3866954394416027
116	50	-0.43380756470656223
117	1	-0.24851745361587518
117	2	-0.26887643229255787
117	3	-0.24559704302834007
117	4	-0.29388984447354005
117	5	-0.2124930000833558
117	6	-0.2340939412864529
117	7	-0.23725888785081395
117	8	-0.33421697760481095
117	9	-0.26219724999804583
117	10	-0.24438453009229733
117	11	-0.2936177939889788
117	12	-0.31614490147800467
117	13	-0.36962905818752745
117	14	-0.3339853991342423
117	15	-0.34522305605097614
117	16	-0.30871621252608605
117	17	-0.2921088785532646
117	18	-0.25430003959849756
117	19	-0.42246447445782565
117	20	-0.2590762853884314
117	21	-0.23400192010957
117	22	-0.1927357037644768
117	23	-0.17445781436207852
117	24	-0.22288896490491084
117	25	-0.3059633476219119
117	26	-0.3208812194163144
117	27	-0.21076158036908055
117	28	-0.2269904011745396
117	29	-0.23157597422217435
117	30	-0.2494165470389902
117	31	-0.2549737108789145
117	32	-0.1810535584038817
117	33	-0.23447774194337256
117	34	-0.26489413334235934
117	35	-0.18819450974392993
117	36	-0.2551118537199031
117	37	-0.3048734694775135
117	38	-0.2269183401032322
117	39	-0.20655022093393427
117	40	-0.30693573655190676
117	41	-0.13014770164537737
117	42	-0.1324119626503446
117	43	-0.12141800166771333
117	44	-0.17997649849320396
117	45	-0.06316581329439686
117	46	-0.11281026732648769
117	47	0.02599448933206361
117	48	0.026524868226339038
117	49	0.07259659331283075
117	50	0.03242627016519606
119	1	-0.3826387212472966
119	2	-0.33348693405014984
119	3	-0.31142479306527804
119	4	-0.34800354530454314
119	5	-0.34085213506018874
119	6	-0.33234117389606155
119	7	-0.33262284972303746
119	8	-0.37667833876051304
119	9	-0.3858800219546268
119	10	-0.40771739854623235
119	11	-0.3482010341697048
119	12	-0.33184389629304434
119	13	-0.2815921973627482
119	14	-0.30217215259030183
119	15	-0.33169759563528345
119	16	-0.26914826518459023
119	17	-0.43976353596580253
119	18	-0.40380985692489446
119	19	-0.3174193433932686
119	20	-0.21881234904271452
119	21	-0.3056254177296793
119	22	-0.3543202517835766
119	23	-0.29224298475777033
119	24	-0.2151355151333334
119	25	-0.25085583919278776
119	26	-0.1388333417870058
119	27	-0.186759967931458
119	28	-0.16906761922314217
119	29	-0.11859121839695774
119	30	-0.15075333201345842
119	31	-0.0831671783914345
119	32	-0.08849001586440863
119	33	-0.07113144705074603
119	34	-0.06467788908344829
119	35	-0.10804888362281417
119	36	-0.07486748583886538
119	37	-0.1874960358878326
119	38	-0.1718742758987588
119	39	-0.06318380619506314
119	40	-0.03762177892293295
119	41	-0.05153912138927552
119	42	-0.09038720491129482
119	43	-0.08529498457179585
119	44	-0.1836834605485933
119	45	-0.19344555684169507
119	46	-0.22934847176247786
119	47	-0.2540078529234151
119	48	-0.4583559293675563
119	49	-0.1407242364576362
119	50	-0.13343229882701735
120	1	-0.3574592589019545
120	2	-0.38731821321270843
120	3	-0.4198442699624678
120	4	-0.454539234471234
120	5	-0.6812068960419353
120	6	-0.5164520025331143
120	7	-0.7079540107638138
120	8	-0.7326146848880484
120	9	-0.7218525639096214
120	10	-0.5590521933977257
120	11	-0.6433539958313688
120	12	-0.6418295839238297
120	13	-0.49856582543282
120	14	-0.5848352276933397
120	15	-0.6644382186277937
120	16	-0.6633406267904078
120	17	-0.6939110317317727
120	18	-0.5684222545309083
120	19	-0.5641252308908165
120	20	-0.510049962500041
120	21	-0.6141806859229106
120	22	-0.5817005047453137
120	23	-0.7887883169614156
120	24	-1.1005031211470282
120	25	-0.9145082373754079
120	26	-1.097949950926349
120	27	-0.8012810218226818
120	28	-0.5730732401444101
120	29	-0.7279881676615076
120	30	-0.8612859274706111
120	31	-0.6808994820004983
120	32	-0.9892275628699423
120	33	-0.7006992876355937
120	34	-0.8321439304558567
120	35	-0.7134184004277415
120	36	-0.4984920121797245
120	37	-0.7498853867150785
120	38	-0.7644373166815015
120	39	-0.7384958156411869
120	40	-0.43676329106364165
120	41	-0.4454614433263657
120	42	-0.36992422993134966
120	43	-0.31716703063995766
120	44	-0.2784090730029689
120	45	-0.5496182401372067
120	46	-0.23371507902581357
120	47	-0.18990762162687957
120	48	-0.25137532756473036
120	49	-0.25268207499268414
120	50	-0.2759001237657799
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	34037.0
3	1672.0
4	2740.0
5	2842.0
6	4215.0
7	7226.0
8	8353.0
9	9800.0
10	13237.0
11	16155.0
12	18870.0
13	23775.0
14	28016.0
15	30717.0
16	33978.0
17	37213.0
18	42240.0
19	49727.0
20	57986.0
21	56550.0
22	69595.0
23	88095.0
24	111110.0
25	128831.0
26	164230.0
27	203896.0
28	245501.0
29	291501.0
30	352981.0
31	426720.0
32	518520.0
33	650446.0
34	869559.0
35	1240518.0
36	1892763.0
37	3227738.0
38	5314866.0
39	2.1067611E7
40	258040.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	16.080651685909416	29.227429129833382	27.72006731655444	26.971851867702757
2	23.89838370987588	30.26932512966758	25.23162034552296	20.60067081493358
3	23.594268124477463	27.587159081291734	26.174680790704848	22.643892003525956
4	24.813027972682235	26.021194196610814	25.363706462174733	23.802071368532214
5	25.06965785506225	28.248328308549652	26.657603501709925	20.024410334678173
6	25.526317070213544	26.514059061443124	23.777740858565647	24.181883009777692
7	23.95067585734433	26.52873646975536	27.265729071453094	22.254858601447218
8	24.558124369878414	26.466263512958264	26.030997926432914	22.94461419073041
9	23.63811427463581	25.864945014702727	26.70851210325444	23.788428607407024
10	22.984891442898984	27.48469424525961	25.83856068860405	23.691853623237353
11	23.738096004267874	26.97722746235759	25.84590340852729	23.43877312484725
12	23.326007456544048	26.194324909904747	26.827242900419577	23.652424733131625
13	23.28218061013658	26.68942583479219	26.251872813787347	23.77652074128388
14	23.232345609249585	27.441674168067838	26.28057481405709	23.045405408625488
15	22.71987753790772	27.11211472630063	26.718391972307735	23.449615763483912
16	23.021574085480605	26.3284576270357	26.65669972385324	23.99326856363046
17	23.552254183103127	26.280804651470792	26.45098767694266	23.715953488483418
18	22.75593668802217	26.474407195516775	26.421500235440494	24.34815588102056
19	23.33444054777063	26.90656076413221	26.421654029440557	23.337344658656605
20	23.70941392010557	26.37372024316876	26.450817472641653	23.46604836408402
21	23.11770664597266	27.05561186185687	26.383155944105972	23.443525548064496
22	23.8350675074372	26.344459530283302	26.381534826857088	23.438938135422408
23	23.20649744281335	26.302292944473237	27.198583474704847	23.29262613800856
24	22.929688483930054	27.04615359162838	26.255861638091073	23.768296286350495
25	23.08672462210609	26.467032465952094	26.27544222895313	24.170800682988688
26	23.26104042495616	26.503739776389157	26.98589793207482	23.249321866579862
27	23.372369775743817	26.314555937159902	26.893593276572407	23.419481010523878
28	23.443264075831188	26.39973122082595	26.487004097488164	23.670000605854696
29	23.68483183778654	26.30346966678843	26.55920830159503	23.452490193829995
30	23.63491176286728	26.348366361798153	26.549076768057887	23.467645107276677
31	23.384175805885107	26.579815571852887	26.559473116290878	23.47653550597113
32	23.518285055815436	26.343594621960175	26.548810630928454	23.589309691295934
33	23.353249869488447	26.56635032714559	26.465107017876367	23.615292785489597
34	23.385756298510717	26.362651603985505	26.687992457296932	23.563599640206842
35	23.246663747009652	26.47763311741874	26.628847959442197	23.646855176129407
36	23.387634055340225	26.346148806541013	26.64206339973888	23.62415373837988
37	23.40167023539543	26.44793003963095	26.427133620430133	23.723266104543487
38	23.26276478194616	26.831902449240495	26.494983667910905	23.41034910090244
39	23.358306641801782	26.541198686305922	26.488720932608455	23.61177373928384
40	23.341068707089622	26.392056236047324	26.522570520513263	23.744304536349798
41	23.269346963591584	26.817874480140773	26.452942314704753	23.45983624156289
42	23.609361422069554	26.357190765365683	26.438746291452798	23.594701521111965
43	23.387996593260635	26.387157062780393	26.46419065458136	23.760655689377614
44	23.428048837134497	26.630933434658743	26.44901246767834	23.49200526052842
45	23.33677166366648	26.522108790960676	26.466640129408574	23.67447941596427
46	23.230726243999953	26.43822786494229	26.68882629702572	23.64221959403203
47	23.496604136319007	26.488187162235427	26.50486715761684	23.510341543828726
48	23.178360492590357	26.37848869347423	26.319604675557883	24.12354613837752
49	23.22922153811375	26.617445867873464	26.279300019833695	23.87403257417909
50	23.60814952446709	26.479989942611294	26.341826165288595	23.57003436763302
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	37099.0
1	20642.5
2	4186.0
3	3307.0
4	2428.0
5	2482.0
6	2536.0
7	3001.0
8	3466.0
9	4850.5
10	6235.0
11	9424.5
12	12614.0
13	18733.0
14	24852.0
15	36627.0
16	48402.0
17	72018.5
18	95635.0
19	133951.0
20	172267.0
21	229300.0
22	286333.0
23	413355.0
24	540377.0
25	552167.0
26	563957.0
27	656748.5
28	749540.0
29	840868.5
30	932197.0
31	1050133.0
32	1168069.0
33	1285392.0
34	1402715.0
35	1534674.0
36	1666633.0
37	1780568.0
38	1894503.0
39	1987240.0
40	2079977.0
41	2153542.0
42	2227107.0
43	2291477.5
44	2355848.0
45	2384505.0
46	2413162.0
47	2392357.0
48	2371552.0
49	2361329.5
50	2351107.0
51	2322261.5
52	2293416.0
53	2243891.5
54	2194367.0
55	2100197.0
56	2006027.0
57	1950275.0
58	1894523.0
59	1779175.5
60	1663828.0
61	1467209.0
62	1270590.0
63	1113546.0
64	956502.0
65	825465.5
66	694429.0
67	594678.5
68	494928.0
69	409123.5
70	323319.0
71	255261.5
72	187204.0
73	149066.0
74	110928.0
75	81988.0
76	53048.0
77	39504.0
78	25960.0
79	18290.0
80	10620.0
81	7735.0
82	4850.0
83	3689.5
84	2529.0
85	1905.5
86	1282.0
87	870.5
88	459.0
89	292.5
90	126.0
91	85.0
92	44.0
93	42.0
94	40.0
95	29.5
96	19.0
97	16.0
98	13.0
99	17.5
100	22.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.07855194435808645
2	3.803002350680963E-4
3	0.010967539646299505
4	6.808172040379906E-4
5	0.0015504548045083926
6	6.622011086150769E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	7.180493948838183E-5
14	1.5956653219640404E-5
15	7.978326609820203E-5
16	0.004185962027952334
17	0.0
18	3.191330643928081E-5
19	0.0
20	0.0
21	0.0
22	3.457274864255421E-5
23	0.0
24	0.014656185982239714
25	0.004874757558600144
26	0.03170586994742549
27	0.06607650098253091
28	0.04946828442308854
29	0.04212556449985067
30	0.0673982437575578
31	0.10646544972364405
32	0.1257783190038155
33	0.12182372844754795
34	0.13671128590147247
35	0.10131676961810676
36	0.1414770063297384
37	0.17701779193428413
38	0.19004373984591724
39	0.1703612080994908
40	0.17689279815073028
41	0.17804699606695093
42	0.20675035576688075
43	0.22464574235270743
44	0.20712533711754227
45	0.21217561786155847
46	0.21404786517266297
47	0.22305805535735324
48	0.22804185004628758
49	0.23921682618444243
50	0.2582451351488636
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	3.760187E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.226050293243354
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.61673625382002	35.879151536664885
2	11.022905170793095	10.190907376054676
3	4.033751809185125	5.593932420055588
4	2.0059351708855266	3.7090584037736027
5	1.1550013846111706	2.669557604690084
6	0.7571364374379888	2.099965622151334
7	0.5124408630822901	1.6581681976408482
8	0.3815308592906669	1.4109331751995777
9	0.2813931035248731	1.1706922580141357
>10	1.8856686599694048	17.340459522779465
>50	0.23329961899305807	7.429571585753536
>100	0.11076604950685212	8.863878891951169
>500	0.0030446043898332354	0.9045239793148894
>1k	3.439643683199812E-4	0.2496467264044197
>5k	5.756267985450706E-6	0.01581636186510763
>10k+	4.029387356687268E-5	0.8137363376864273
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	111731	0.29714213681394036	No Hit
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAG	69387	0.18453071615853148	Illumina Paired End PCR Primer 2 (97% over 36bp)
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCG	60085	0.1597925847836823	Illumina Paired End PCR Primer 2 (100% over 31bp)
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.951980845633475E-4	2.659442203273401E-6	0.0	0.0	0.0
2	3.005169689698943E-4	1.0637768813093603E-5	0.0	2.659442203273401E-6	0.0
3	3.031764111731677E-4	1.0637768813093603E-5	0.0	5.318884406546802E-6	0.0
4	3.0583585337644114E-4	1.3297211016367005E-5	0.0	7.978326609820204E-6	0.0
5	3.084952955797145E-4	1.5956653219640408E-5	2.659442203273401E-6	7.978326609820204E-6	0.0
6	3.111547377829879E-4	1.861609542291381E-5	2.659442203273401E-6	7.978326609820204E-6	0.0
7	3.2179250659608154E-4	1.861609542291381E-5	5.318884406546802E-6	1.0637768813093603E-5	0.0
8	3.2977083320590174E-4	2.1275537626187207E-5	5.318884406546802E-6	1.3297211016367005E-5	0.0
9	3.5636525523863576E-4	2.1275537626187207E-5	5.318884406546802E-6	1.5956653219640408E-5	0.0
10	5.824178425168748E-4	2.1275537626187207E-5	5.318884406546802E-6	1.5956653219640408E-5	0.0
11	6.914549728510843E-4	2.3934979829460608E-5	5.318884406546802E-6	1.861609542291381E-5	0.0
12	8.802753692834957E-4	2.3934979829460608E-5	5.318884406546802E-6	1.861609542291381E-5	0.0
13	0.0010903713033420944	2.925386423600741E-5	5.318884406546802E-6	1.861609542291381E-5	0.0
14	0.001154197916220656	2.925386423600741E-5	5.318884406546802E-6	1.861609542291381E-5	0.0
15	0.009129865083837585	3.1913306439280815E-5	5.318884406546802E-6	1.861609542291381E-5	0.0
16	0.009347939344506005	3.1913306439280815E-5	5.318884406546802E-6	1.861609542291381E-5	0.0
17	0.010002162126511262	3.457274864255421E-5	5.318884406546802E-6	1.861609542291381E-5	0.0
18	0.010443629532254646	3.9891633049101016E-5	5.318884406546802E-6	1.861609542291381E-5	0.0
19	0.011600486890678574	4.521051745564782E-5	5.318884406546802E-6	2.1275537626187207E-5	0.0
20	0.17986073564958338	4.521051745564782E-5	5.318884406546802E-6	2.1275537626187207E-5	0.0
21	0.1833791776845141	4.521051745564782E-5	5.318884406546802E-6	2.1275537626187207E-5	0.0
22	0.1840280815821128	5.052940186219462E-5	5.318884406546802E-6	2.3934979829460608E-5	0.0
23	0.1846291155200526	5.584828626874142E-5	7.978326609820204E-6	2.3934979829460608E-5	0.0
24	0.18547215869849026	5.850772847201482E-5	7.978326609820204E-6	2.659442203273401E-5	0.0
25	0.18601202546575477	5.850772847201482E-5	7.978326609820204E-6	3.1913306439280815E-5	0.0
26	0.18679922035792368	5.850772847201482E-5	7.978326609820204E-6	3.1913306439280815E-5	0.0
27	0.18757045859687296	6.116717067528823E-5	7.978326609820204E-6	3.457274864255421E-5	0.0
28	0.2165530597281465	6.382661287856163E-5	7.978326609820204E-6	3.723219084582762E-5	0.0
29	0.21840935038603132	6.648605508183502E-5	7.978326609820204E-6	3.723219084582762E-5	0.0
30	0.22411917279645932	6.914549728510843E-5	7.978326609820204E-6	4.2551075252374413E-5	0.0
31	0.417960596108651	6.914549728510843E-5	7.978326609820204E-6	4.521051745564782E-5	0.0
32	0.4202610136144825	6.914549728510843E-5	7.978326609820204E-6	5.052940186219462E-5	0.0
33	0.4315237513453453	6.914549728510843E-5	7.978326609820204E-6	5.052940186219462E-5	0.0
34	0.43269124647258234	6.914549728510843E-5	1.3297211016367005E-5	5.318884406546802E-5	0.0
35	0.4340714969760812	7.446438169165524E-5	1.3297211016367005E-5	5.318884406546802E-5	0.0
36	0.4355368496300849	7.978326609820203E-5	1.3297211016367005E-5	5.584828626874142E-5	0.0
37	0.4372176171025537	7.978326609820203E-5	1.3297211016367005E-5	5.584828626874142E-5	0.0
38	0.4391989015439924	7.978326609820203E-5	1.3297211016367005E-5	5.584828626874142E-5	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCAACGC	38640	0.0	29.713158	12
ATCAACG	39280	0.0	29.23459	11
CAACGCA	39760	0.0	28.726923	13
ACGCAGA	40800	0.0	27.795364	15
AACGCAG	41050	0.0	27.781347	14
AAGCAGT	43480	0.0	27.319805	1
GTATCAA	42435	0.0	27.241247	9
GGTATCA	42605	0.0	27.106726	8
GTGGTAT	43310	0.0	26.736734	6
CGCAGAG	42575	0.0	26.590044	16
TGGTATC	43960	0.0	26.266203	7
AGTGGTA	44730	0.0	26.06033	5
TATCAAC	44995	0.0	25.785149	10
AGAGTAC	42825	0.0	25.504534	19
CAGAGTA	45535	0.0	24.149675	18
AGCAGTG	49340	0.0	24.078188	2
GCAGAGT	46775	0.0	23.844473	17
TCGTATG	3170	0.0	23.212801	40
GAGTACT	26305	0.0	23.07477	20
TATGCCG	3300	0.0	22.233818	43
>>END_MODULE