FastQCFastQC Report
Thu 26 May 2016
SRR522099_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR522099_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33972212
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAG1441350.4242732266006111Illumina Paired End PCR Primer 2 (97% over 36bp)
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT488510.14379693615476083No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGA250450.035.02282725
TGCCGAG257100.034.1193626
AGATCGG230050.034.05611831
AATGCCG261500.033.7173424
CGAGATC253800.031.57993529
GAGATCG257600.030.96062530
CCGAGAT267200.030.0754728
GCCGAGA305400.029.1714427
TATGCCG28300.027.06604643
GTTCAGC343050.026.05182614
GAATGCC351600.025.41429323
GGAATGC360400.025.09823622
CAGGAAT382300.023.71425620
GACCGAT32950.023.4434932
TCGTATG32500.022.9568640
AGGAATG397050.022.89084621
CGTATGC32900.022.67875341
CTCGTAT34350.022.03968839
GCAGGAA419450.022.00084519
ACCGATC35250.021.60190433
Produced by FastQC (version 0.11.5)