FastQCFastQC Report
Thu 26 May 2016
SRR522098_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR522098_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38912248
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1332360.3424011894661033No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC428250.030.55526512
CAACGCA432500.030.17369513
ATCAACG441400.029.74955411
AACGCAG448650.029.08753814
ACGCAGA455150.028.57551415
AAGCAGT488600.028.000681
CGCAGAG462200.027.97795116
GTATCAA476350.027.705079
GGTATCA481900.027.4361698
GTGGTAT483950.027.356256
AGTGGTA496900.026.7538935
TGGTATC499250.026.513497
TATCAAC501800.026.3964810
AGAGTAC479600.026.1729719
CAGAGTA501350.025.34396418
GAGTACT309100.025.32280720
GCAGAGT512500.025.00025217
AGCAGTG556700.024.5221182
GTACTTT328500.023.42402622
CAGTGGT590000.022.7494584
Produced by FastQC (version 0.11.5)