##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR522098_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	38912248
Sequences flagged as poor quality	0
Sequence length	50
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.65797727748857	40.0	39.0	40.0	36.0	40.0
2	38.499574375656735	40.0	39.0	40.0	35.0	40.0
3	38.482861848536736	40.0	39.0	40.0	35.0	40.0
4	38.46619838052019	40.0	39.0	40.0	35.0	40.0
5	38.48186858800859	40.0	39.0	40.0	35.0	40.0
6	38.522513811075626	40.0	39.0	40.0	35.0	40.0
7	38.49646126844175	40.0	39.0	40.0	35.0	40.0
8	38.46527777063921	40.0	39.0	40.0	35.0	40.0
9	38.46218532529912	40.0	38.0	40.0	35.0	40.0
10	38.44552383609397	40.0	38.0	40.0	35.0	40.0
11	38.47987543664915	40.0	39.0	40.0	35.0	40.0
12	38.43495610944914	40.0	38.0	40.0	35.0	40.0
13	38.40922472533584	40.0	38.0	40.0	35.0	40.0
14	38.38207761730959	40.0	38.0	40.0	35.0	40.0
15	38.36742608651137	40.0	38.0	40.0	35.0	40.0
16	38.377581911998504	40.0	38.0	40.0	35.0	40.0
17	38.31026464983467	40.0	38.0	40.0	35.0	40.0
18	38.26645792861929	40.0	38.0	40.0	35.0	40.0
19	38.21946986974384	40.0	38.0	40.0	35.0	40.0
20	38.16817748488856	40.0	38.0	40.0	35.0	40.0
21	38.169284771211366	40.0	38.0	40.0	35.0	40.0
22	38.11758899151753	40.0	38.0	40.0	35.0	40.0
23	38.04254310879186	40.0	38.0	40.0	35.0	40.0
24	37.99275968327505	40.0	38.0	40.0	34.0	40.0
25	37.97761059705417	40.0	38.0	40.0	34.0	40.0
26	37.8777799216329	40.0	38.0	40.0	34.0	40.0
27	37.80958961815827	40.0	38.0	40.0	33.0	40.0
28	37.74875918245587	39.0	38.0	40.0	33.0	40.0
29	37.68955147489808	39.0	38.0	40.0	33.0	40.0
30	37.62080073091639	39.0	38.0	40.0	33.0	40.0
31	37.5507487513957	39.0	38.0	40.0	33.0	40.0
32	37.47537973133806	39.0	38.0	40.0	33.0	40.0
33	37.40703084026397	39.0	38.0	40.0	33.0	40.0
34	37.33947802244681	39.0	38.0	40.0	33.0	40.0
35	37.27263608620093	39.0	37.0	40.0	33.0	40.0
36	37.168405459381326	39.0	37.0	40.0	33.0	40.0
37	37.05564073296408	39.0	37.0	40.0	33.0	40.0
38	36.97014009573541	39.0	37.0	40.0	32.0	40.0
39	37.14646550361213	39.0	37.0	40.0	33.0	40.0
40	37.215481819503204	39.0	38.0	40.0	33.0	40.0
41	37.42244315979894	39.0	38.0	40.0	33.0	40.0
42	37.36473084258714	39.0	38.0	40.0	33.0	40.0
43	37.32041024717976	39.0	38.0	40.0	33.0	40.0
44	37.23967358555075	39.0	38.0	40.0	33.0	40.0
45	37.169874020128574	39.0	38.0	40.0	33.0	40.0
46	37.09688409675021	39.0	38.0	40.0	33.0	40.0
47	37.007187351396404	39.0	37.0	40.0	33.0	40.0
48	36.88736507847092	39.0	37.0	40.0	33.0	40.0
49	36.79911502414355	39.0	37.0	40.0	32.0	40.0
50	36.69480963937113	39.0	37.0	40.0	32.0	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1	1	-0.2632257927508377
1	2	-0.31386544243132164
1	3	-0.30460571701097194
1	4	-0.3080415447320135
1	5	-0.2887025657768376
1	6	-0.32056109082980555
1	7	-0.3142547052835738
1	8	-0.304706843599881
1	9	-0.32822256533161465
1	10	-0.3232688950465672
1	11	-0.32169283121645265
1	12	-0.359105520993765
1	13	-0.3279124704664298
1	14	-0.32294234144691103
1	15	-0.3672579441014179
1	16	-0.33372418885625876
1	17	-0.3169018415977405
1	18	-0.31207832928995316
1	19	-0.3244335937343692
1	20	-0.35028177576548813
1	21	-0.32457470142346523
1	22	-0.3440791182548537
1	23	-0.28220440584965445
1	24	-0.2725514909027922
1	25	-0.36282267031087656
1	26	-0.3676881341345677
1	27	-0.29720127076127056
1	28	-0.3219336956625938
1	29	-0.27480995717143486
1	30	-0.26372964545215893
1	31	-0.28977383701971604
1	32	-0.3052306104191871
1	33	-0.26917629979120505
1	34	-0.29475767291766886
1	35	-0.2906101556455667
1	36	-0.31045403953134354
1	37	-0.31906279391436954
1	38	-0.2684700912865168
1	39	-0.313399455589483
1	40	-0.2401240995755387
1	41	-0.22144088492292724
1	42	-0.2537753733124788
1	43	-0.275274524336794
1	44	-0.2849302845505477
1	45	-0.2749136706820039
1	46	-0.2279986812164907
1	47	-0.20984811696161643
1	48	-0.2580094640298185
1	49	-0.22353658526574094
1	50	-0.25916092045573436
2	1	-0.2174027623747108
2	2	-0.263502662721649
2	3	-0.2680198442929509
2	4	-0.2528676796031064
2	5	-0.24849626144747816
2	6	-0.2515577296910294
2	7	-0.2694996224665047
2	8	-0.28786266153058193
2	9	-0.291779114633151
2	10	-0.28872857479389324
2	11	-0.33455802436843385
2	12	-0.30761950603677946
2	13	-0.2932206603415821
2	14	-0.29831498103162346
2	15	-0.3294575815139211
2	16	-0.3032073300845042
2	17	-0.2841472720566145
2	18	-0.3425455737138847
2	19	-0.31093857801621283
2	20	-0.2962119821138671
2	21	-0.28088667318020555
2	22	-0.2998669938317349
2	23	-0.2812675745182176
2	24	-0.32647305411489214
2	25	-0.27355268485040796
2	26	-0.35760209433448154
2	27	-0.2665890589812676
2	28	-0.338409491746944
2	29	-0.2750043490338143
2	30	-0.2852224078801271
2	31	-0.28231662019287285
2	32	-0.33820566042678024
2	33	-0.34721108077499707
2	34	-0.3574495939654483
2	35	-0.3479836486072898
2	36	-0.4019298219434546
2	37	-0.36385222490141444
2	38	-0.34087726465436674
2	39	-0.46359514023602344
2	40	-0.38047216126012273
2	41	-0.3777997266494566
2	42	-0.40048665070843015
2	43	-0.4236609537026794
2	44	-0.3685215650930189
2	45	-0.39449995320285325
2	46	-0.29861292056220634
2	47	-0.3630855670983095
2	48	-0.44735106917041634
2	49	-0.2965082268004906
2	50	-0.34944411267657927
3	1	-0.2390847015816817
3	2	-0.2644909485555118
3	3	-0.2562711836460565
3	4	-0.27263886342645804
3	5	-0.2556192533016741
3	6	-0.2526005961256246
3	7	-0.2766910404450016
3	8	-0.26722950060396755
3	9	-0.2710273540878063
3	10	-0.27821693259928537
3	11	-0.2728292493667652
3	12	-0.26449166118585055
3	13	-0.2832978728515414
3	14	-0.2919853924156399
3	15	-0.2720532275013525
3	16	-0.2677822811945276
3	17	-0.2846245536921046
3	18	-0.33778800051000957
3	19	-0.24351619322864337
3	20	-0.28935008877813573
3	21	-0.2159147015526912
3	22	-0.2636429667066764
3	23	-0.2778388657532318
3	24	-0.18936916461405673
3	25	-0.2933219029832017
3	26	-0.20431846915656138
3	27	-0.289593757979695
3	28	-0.2792417507853244
3	29	-0.27299435323949695
3	30	-0.3990122669442755
3	31	-0.36157810807689117
3	32	-0.28638208733472226
3	33	-0.2628168882162143
3	34	-0.3177458476296806
3	35	-0.28580859684209514
3	36	-0.3013673443537428
3	37	-0.285384005072828
3	38	-0.3495357902994414
3	39	-0.3118347257937586
3	40	-0.26294827610867344
3	41	-0.22208872183174577
3	42	-0.2663403890160083
3	43	-0.2890067807642964
3	44	-0.2901769592039187
3	45	-0.25596085326147744
3	46	-0.2206480770775201
3	47	-0.3011510823207715
3	48	-0.3210534398020286
3	49	-0.21196111149397723
3	50	-0.23911494824789514
4	1	-0.19401126312238404
4	2	-0.21940306487985595
4	3	-0.2421644240720653
4	4	-0.22020379550967561
4	5	-0.2638213156385092
4	6	-0.22016020803699377
4	7	-0.23621263154854688
4	8	-0.2441507774834264
4	9	-0.2548759367597384
4	10	-0.23538936872147076
4	11	-0.20855569003415297
4	12	-0.24422037173260946
4	13	-0.2496294437778488
4	14	-0.23358963514876052
4	15	-0.2714271024731616
4	16	-0.22343714876393506
4	17	-0.22849643540010334
4	18	-0.21956572094668303
4	19	-0.33680403484217436
4	20	-0.2101762702028438
4	21	-0.28386512706177314
4	22	-0.3208686629343518
4	23	-0.2586603012788373
4	24	-0.20657074530848263
4	25	-0.23249679623624075
4	26	-0.24015529338375075
4	27	-0.2671117093552198
4	28	-0.30613886992351524
4	29	-0.33973675203197473
4	30	-0.30006291300639276
4	31	-0.3069781630846009
4	32	-0.2006074882949278
4	33	-0.2878703146058612
4	34	-0.27933206915685105
4	35	-0.2014513055892735
4	36	-0.2360175666324622
4	37	-0.2619343423753193
4	38	-0.2842610666419816
4	39	-0.3123414077656079
4	40	-0.2867061264497508
4	41	-0.2282406866161324
4	42	-0.1898252850863429
4	43	-0.2496627365979407
4	44	-0.3103971057868691
4	45	-0.2729406442463116
4	46	-0.22207654451097625
4	47	-0.26130887504202605
4	48	-0.24124498922447657
4	49	-0.21152159242409851
4	50	-0.26963037048933103
5	1	-0.15912742326306528
5	2	-0.2117765825643545
5	3	-0.1925780145970748
5	4	-0.1856230178821292
5	5	-0.20643049329562757
5	6	-0.20341972419204524
5	7	-0.21481414398541432
5	8	-0.19755398168118887
5	9	-0.2536425915727776
5	10	-0.21692409581437033
5	11	-0.2181177008209616
5	12	-0.22261251300900398
5	13	-0.18878413314163112
5	14	-0.2326249227869539
5	15	-0.2098200751789676
5	16	-0.1732943860843008
5	17	-0.17648345143817323
5	18	-0.1797059487758048
5	19	-0.14945766078560752
5	20	-0.13243475737665733
5	21	-0.14538411483100333
5	22	-0.12364100065671124
5	23	-0.14401049366139773
5	24	-0.15976310335428678
5	25	-0.20498785560443622
5	26	-0.13099039688477632
5	27	-0.15984192461994695
5	28	-0.11427929361598643
5	29	-0.1614095142627079
5	30	-0.11380307396406408
5	31	-0.11765586325690691
5	32	-0.07822559091206216
5	33	-0.11955121432583127
5	34	-0.08038305299461257
5	35	-0.07281485235070306
5	36	-0.09706905002654764
5	37	-0.043793969337158956
5	38	-0.04405101698542069
5	39	-0.10558527134978135
5	40	-0.020799037056470127
5	41	-0.027382255154300594
5	42	-0.09463790284670637
5	43	-0.05064039783241725
5	44	-0.04957629165405564
5	45	-0.06818531437911446
5	46	-0.03127999068885856
5	47	-0.04588212730180885
5	48	-0.03291536682093721
5	49	0.11114587689671396
5	50	0.03603793835393532
6	1	-0.18940286146258956
6	2	-0.22167930258862611
6	3	-0.2004852869031737
6	4	-0.2064158343919118
6	5	-0.2186200747547744
6	6	-0.19892633263648207
6	7	-0.22880471956642623
6	8	-0.21058086283888855
6	9	-0.26069699230570365
6	10	-0.2053744890639706
6	11	-0.23651111938725933
6	12	-0.21273914559226625
6	13	-0.20891201029381534
6	14	-0.2057568025826768
6	15	-0.22020227579085372
6	16	-0.24317639841905958
6	17	-0.21195228134124022
6	18	-0.21486672487198888
6	19	-0.219700629174163
6	20	-0.21468825473863973
6	21	-0.26661632232525534
6	22	-0.19973797684246364
6	23	-0.15696340390104524
6	24	-0.17091473737288965
6	25	-0.2466029495576123
6	26	-0.16835303153926162
6	27	-0.20877371060814198
6	28	-0.1685428518623553
6	29	-0.22454813230949355
6	30	-0.17011067245233846
6	31	-0.1874742693735456
6	32	-0.13268530212017993
6	33	-0.2142556162846816
6	34	-0.15317291582218218
6	35	-0.16447205509773255
6	36	-0.10918497501419466
6	37	-0.13343269905497124
6	38	-0.1956534991569825
6	39	-0.22186592061019894
6	40	-0.11962971648010523
6	41	-0.05943003898770627
6	42	-0.10034100939935797
6	43	-0.05552838520454628
6	44	-0.04302084161788855
6	45	-0.0786252038967703
6	46	-0.04028758218762363
6	47	-0.11371787494860541
6	48	-0.10356984870183084
6	49	-0.04428617533781676
6	50	-0.027413107917652724
7	1	-0.14885560857178604
7	2	-0.17327916931927945
7	3	-0.1565417022844855
7	4	-0.15015680594587621
7	5	-0.16569134735025415
7	6	-0.15941373206821652
7	7	-0.17964021499877703
7	8	-0.15168714783174408
7	9	-0.16739264897850603
7	10	-0.16253359390522348
7	11	-0.1736484233295812
7	12	-0.20529645804963081
7	13	-0.20951841855359277
7	14	-0.16902087060739035
7	15	-0.2104897136567203
7	16	-0.1695371768663989
7	17	-0.15368257715914524
7	18	-0.15973231336661797
7	19	-0.16450304520721204
7	20	-0.17669244688214292
7	21	-0.17306867460542463
7	22	-0.1296509752810877
7	23	-0.1153169529117406
7	24	-0.1334184265041216
7	25	-0.11744530545825427
7	26	-0.13063090724710946
7	27	-0.15213689316870926
7	28	-0.13027408239948102
7	29	-0.08643222184723243
7	30	-0.15721681502589036
7	31	-0.09582046773608965
7	32	-0.16312421414828293
7	33	-0.0873444229983491
7	34	-0.059593413130116346
7	35	-0.0872810189434361
7	36	-0.1181037910425573
7	37	-0.054586308916931614
7	38	-0.11525369914526351
7	39	-0.08936214705315138
7	40	-0.10928242574731684
7	41	-0.08058573296241889
7	42	-0.062145658735381915
7	43	-0.13870346683003731
7	44	-0.09505635897134113
7	45	-0.11932673487971357
7	46	-0.059353380911396414
7	47	-0.06408187033136414
7	48	-0.059083039672295
7	49	-0.1569111138948145
7	50	0.042838101504869996
8	1	-0.10413916077114749
8	2	-0.11814461800172893
8	3	-0.1416813821204812
8	4	-0.1371401181222538
8	5	-0.1263370772052852
8	6	-0.11498741561145209
8	7	-0.14571767345273656
8	8	-0.12413774284824797
8	9	-0.15235911937581648
8	10	-0.14399059411307036
8	11	-0.1366987336978127
8	12	-0.1478548363318808
8	13	-0.1593374436320616
8	14	-0.16201119990881097
8	15	-0.14937208844153105
8	16	-0.1487318182135482
8	17	-0.13594275357769448
8	18	-0.14351760573666894
8	19	-0.13558867711105904
8	20	-0.1360756963672216
8	21	-0.16216023318533956
8	22	-0.10754289947275453
8	23	-0.13327617194954655
8	24	-0.11705470772847804
8	25	-0.11418346870530627
8	26	-0.13512460501188173
8	27	-0.1624073566131088
8	28	-0.1818349590038153
8	29	-0.20398816873414205
8	30	-0.16572524030438274
8	31	-0.11187381154019249
8	32	-0.11932649109245119
8	33	-0.18755890128068842
8	34	-0.14988525315290246
8	35	-0.1376784648758118
8	36	-0.10285309110509644
8	37	-0.15125835840164825
8	38	-0.15678276712716865
8	39	-0.13635295328582941
8	40	-0.15685432343936867
8	41	-0.033198223934363114
8	42	-0.08734906020161759
8	43	-0.18476704383427034
8	44	-0.10954496970221328
8	45	-0.1163889960856821
8	46	-0.07780971738807096
8	47	-0.16823464760526718
8	48	-0.11985734509511303
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115	28	-0.12077772010044896
115	29	-0.1070742250306509
115	30	-0.1532468266264786
115	31	-0.1740897341994483
115	32	-0.1976347316432907
115	33	-0.1641821852997225
115	34	-0.2774241311556125
115	35	-0.200474626635625
115	36	-0.18211947461404776
115	37	-0.1179705381605416
115	38	-0.13974443802002412
115	39	-0.1848923037939798
115	40	-0.145984333665524
115	41	-0.07866726632668986
115	42	-0.080398425848756
115	43	-0.13248487530463393
115	44	-0.04952339040553966
115	45	-0.11715311349808388
115	46	-0.06941154130407057
115	47	-0.09233928491438093
115	48	0.025265528034431384
115	49	0.04557043504678404
115	50	0.0917232885840562
116	1	-0.20219257253457812
116	2	-0.2747128197232698
116	3	-0.25172397573319927
116	4	-0.28673395499334475
116	5	-0.27081775492252547
116	6	-0.23189235562126953
116	7	-0.23993597965716162
116	8	-0.2445005543434533
116	9	-0.2574133863649948
116	10	-0.23242547718717077
116	11	-0.2688721616108296
116	12	-0.24732455980196022
116	13	-0.26213906254377406
116	14	-0.2412752917794876
116	15	-0.26624374426096153
116	16	-0.21000189460978902
116	17	-0.26798657005266335
116	18	-0.31688912887185694
116	19	-0.24381362860835765
116	20	-0.28172995824911595
116	21	-0.22862502509169502
116	22	-0.22424295795460125
116	23	-0.31093609976315406
116	24	-0.33889183090991537
116	25	-0.2857130363058218
116	26	-0.44390846918371807
116	27	-0.33003598102173015
116	28	-0.3695278168243945
116	29	-0.36110239506120223
116	30	-0.3384057327699992
116	31	-0.44660466810415755
116	32	-0.45300147505207633
116	33	-0.2663643494825507
116	34	-0.3225510786828849
116	35	-0.3254792685297119
116	36	-0.31243262170592345
116	37	-0.36612941790634324
116	38	-0.2701027038643389
116	39	-0.3103092000893426
116	40	-0.3412895041204891
116	41	-0.2449472563446662
116	42	-0.2977513884634533
116	43	-0.252471544945287
116	44	-0.2963530324772137
116	45	-0.2327396317189354
116	46	-0.44477003218863587
116	47	-0.3664960063687772
116	48	-0.33085523900020775
116	49	-0.3361814663539562
116	50	-0.4391162059322511
117	1	-0.12830250186425474
117	2	-0.17039047476951907
117	3	-0.15910914042787283
117	4	-0.17007866458572352
117	5	-0.16034531826150555
117	6	-0.16442120448714093
117	7	-0.17087354063146165
117	8	-0.17166813678454673
117	9	-0.18148476075460707
117	10	-0.15075998468891072
117	11	-0.23434145297182596
117	12	-0.1816510835835814
117	13	-0.19476095656300885
117	14	-0.1820422458914237
117	15	-0.20798149691993473
117	16	-0.2000470248448707
117	17	-0.19739789841217714
117	18	-0.16102767247413396
117	19	-0.20786618865610507
117	20	-0.1573963481063032
117	21	-0.16777686655991886
117	22	-0.17591944878545718
117	23	-0.1820964200062889
117	24	-0.1488039783985471
117	25	-0.1607098027748748
117	26	-0.11243357470261373
117	27	-0.09636475332143135
117	28	-0.06508293306850987
117	29	-0.08640493214968359
117	30	-0.08790723409000378
117	31	-0.03494973999625017
117	32	-0.05226130058661482
117	33	-0.06361769814643026
117	34	-0.08041450904907776
117	35	-0.05167305209172213
117	36	-0.053120268963802175
117	37	-0.06624084970999888
117	38	-0.0709620826282773
117	39	-0.12021923796950063
117	40	-0.11474604844573122
117	41	-0.10388082678116461
117	42	-0.0328531711222837
117	43	-0.03222789706656215
117	44	-0.060250646290050724
117	45	-0.07763474342792875
117	46	-0.06885124059929382
117	47	0.0648978971776657
117	48	0.06382504604864891
117	49	0.09181009550062669
117	50	0.11621446696241833
118	1	-0.2357681059796377
118	2	-0.2459646303529368
118	3	-0.22535140590780856
118	4	-0.2573788755351387
118	5	-0.19651158674376745
118	6	-0.20373578573266116
118	7	-0.2106948148919372
118	8	-0.22618917935402294
118	9	-0.23535707185720867
118	10	-0.2225578093315761
118	11	-0.23162266434420076
118	12	-0.22876677002553691
118	13	-0.2425788442148189
118	14	-0.2332247824592244
118	15	-0.2530425036852151
118	16	-0.22591153758836668
118	17	-0.18903939888149068
118	18	-0.238541197452399
118	19	-0.18290844859144784
118	20	-0.1921314145171351
118	21	-0.2441133974767098
118	22	-0.22119689445829493
118	23	-0.22031697352709045
118	24	-0.13492879081587716
118	25	-0.20700451533404873
118	26	-0.18812155136738795
118	27	-0.1550204359114815
118	28	-0.1484007717673208
118	29	-0.1299936081048827
118	30	-0.14547786436658328
118	31	-0.07342296765051515
118	32	-0.0706094343289223
118	33	-0.06382720035817613
118	34	-0.12024229111561624
118	35	-0.044409825257346824
118	36	-0.08987876715778498
118	37	-0.054477897913088213
118	38	-0.10930020852642741
118	39	-0.1369873032042932
118	40	-0.07069409413697514
118	41	-0.14384229735257037
118	42	-0.17275919965359776
118	43	-0.12488274425088974
118	44	-0.006006886891853469
118	45	-0.037245796371536244
118	46	-0.09020587501142074
118	47	-0.030728923563508204
118	48	-0.004212662068013628
118	49	-0.027951993286741583
118	50	0.0011889396240150063
119	1	-0.19530989520352904
119	2	-0.24376490408985774
119	3	-0.2280871908660984
119	4	-0.26247463862193143
119	5	-0.26821880150821187
119	6	-0.2489869831757332
119	7	-0.2434954836923069
119	8	-0.26277890995079645
119	9	-0.2907508262061853
119	10	-0.2930679210789151
119	11	-0.2718986929202458
119	12	-0.2679930075956918
119	13	-0.24852609227925626
119	14	-0.28055596782867553
119	15	-0.24585026097732054
119	16	-0.27274057696009635
119	17	-0.28035852499807135
119	18	-0.30209965937322636
119	19	-0.25922989506337046
119	20	-0.26212268758042967
119	21	-0.35463232057723815
119	22	-0.3412940567952134
119	23	-0.315632838423646
119	24	-0.2621086784290583
119	25	-0.26480099078951014
119	26	-0.2061355379472829
119	27	-0.2524068591874382
119	28	-0.21507606238289156
119	29	-0.2141078676092647
119	30	-0.17008865269482243
119	31	-0.15135598704030429
119	32	-0.2193361023170297
119	33	-0.31613652674870707
119	34	-0.23221972794963364
119	35	-0.313190651537802
119	36	-0.21349802252650107
119	37	-0.2004428174262074
119	38	-0.18780269200907895
119	39	-0.3024221457399605
119	40	-0.199496836897751
119	41	-0.3381261038942327
119	42	-0.25731238625093056
119	43	-0.21324984662833657
119	44	-0.2303071879910661
119	45	-0.2129098795603781
119	46	-0.14343902525979502
119	47	-0.1683152077565353
119	48	-0.22124419309214005
119	49	-0.24044039542232554
119	50	-0.14145174343569522
120	1	-0.24426122375646742
120	2	-0.2973524428393688
120	3	-0.3380243862967305
120	4	-0.345468272001483
120	5	-0.33078676218379144
120	6	-0.34039910340214874
120	7	-0.35804246344391544
120	8	-0.34025236507314816
120	9	-0.3935202606142596
120	10	-0.3456892278840584
120	11	-0.357543118718219
120	12	-0.32364563854610395
120	13	-0.38769952281055
120	14	-0.37936339392076235
120	15	-0.43245383370749835
120	16	-0.34660645366774645
120	17	-0.3914026767922749
120	18	-0.3861242244815557
120	19	-0.3731239022526367
120	20	-0.47888849151129165
120	21	-0.37492341185119216
120	22	-0.382659552027647
120	23	-0.4475684719772346
120	24	-0.4717478455637618
120	25	-0.3882213707204585
120	26	-0.44458582990019124
120	27	-0.5575469010441196
120	28	-0.4220655848682142
120	29	-0.39365116325741667
120	30	-0.4023499183160766
120	31	-0.42668911083598715
120	32	-0.44635809255763803
120	33	-0.3950897544846441
120	34	-0.43353011131051744
120	35	-0.44069365473205124
120	36	-0.42335711528723863
120	37	-0.42874702880240534
120	38	-0.4447484368787755
120	39	-0.4366532365280662
120	40	-0.4101370445343093
120	41	-0.3394960117915673
120	42	-0.38591462812748745
120	43	-0.3989831907251258
120	44	-0.36205950034519674
120	45	-0.43679819287469
120	46	-0.3218517889863719
120	47	-0.35223681754184355
120	48	-0.35437993232866205
120	49	-0.4113488725401879
120	50	-0.3582448388451738
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	25755.0
3	1544.0
4	2610.0
5	2867.0
6	4361.0
7	7203.0
8	8489.0
9	10131.0
10	13821.0
11	16405.0
12	19691.0
13	24769.0
14	27952.0
15	30479.0
16	34045.0
17	36678.0
18	40613.0
19	46264.0
20	52509.0
21	55139.0
22	67849.0
23	87569.0
24	110480.0
25	128758.0
26	164386.0
27	202305.0
28	245919.0
29	291085.0
30	352103.0
31	429439.0
32	525019.0
33	662946.0
34	892932.0
35	1282734.0
36	1973525.0
37	3365024.0
38	5460241.0
39	2.1957678E7
40	250931.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	14.187120384612545	31.66898465340104	24.45121399384867	29.692680968137747
2	23.603169813314633	31.053828571175984	25.822366028650713	19.520635586858674
3	22.548215739256516	28.805080586128877	26.00227117706076	22.644432497553847
4	23.409754056478775	27.185498451777462	25.981341644808637	23.42340584693513
5	24.298765776780616	29.14229730443741	27.103216961405057	19.455719957376914
6	24.515692864653715	27.22487647138475	24.683408734203137	23.576021929758397
7	23.59822028272435	26.973139665433877	27.726342101849266	21.702297949992506
8	23.389728087670495	26.82066839212168	27.10623914609097	22.68336437411686
9	22.646399149183054	26.286851893008084	27.961165337967625	23.105583619841237
10	22.454554668751083	27.748222102202885	26.514708171062235	23.2825150579838
11	22.668353676199843	27.782244808883828	26.51674865970221	23.032652855214124
12	22.565470902631994	26.975038810402317	27.375596495992728	23.083893790972958
13	22.590534990422555	27.32392895933435	26.589938982707963	23.495597067535137
14	22.62678616162967	28.132397099399025	26.647901692147443	22.59291504682386
15	22.342093716252574	27.918112420502496	26.623696624294897	23.116097238950044
16	22.555029219899698	27.133160870722456	26.679051703549924	23.632758205827923
17	23.374537497807886	27.0587887906142	26.727720793720273	22.83895291785764
18	22.434943398645018	26.980380538962155	26.683933902427746	23.900742159965077
19	22.644903476149718	27.734167401482434	26.661497428778723	22.959431693589124
20	23.46529812412791	27.0272897109414	26.775754513077732	22.73165765185296
21	22.71266877205347	27.5585157660385	26.715665463480804	23.01314999842723
22	23.304526797407725	26.90429698793444	26.738480242486208	23.05269597217163
23	22.632735070973027	27.10812801151966	27.502566801075073	22.756570116432233
24	22.557165274141493	27.57389269356456	26.722213806352396	23.14672822594155
25	22.560115753169143	26.99618038390652	26.674989599546294	23.768714263378037
26	22.699844601332565	27.27620233469436	27.315936890995886	22.708016172977185
27	22.742689885758654	26.83946573190237	27.451174844401955	22.966669537937026
28	23.01965522236023	26.92182331119049	27.02704266089701	23.031478805552265
29	23.105298615154744	27.026610198101885	27.149801347010875	22.718289839732495
30	23.013644812544147	26.865033087763575	27.123163737930483	22.998158361761796
31	22.87698591854358	27.034796183802147	27.023837901988728	23.06437999566555
32	22.959542728062544	27.069828084660795	27.108514103906973	22.862115083369684
33	22.819021466950655	26.977863072134145	27.026158849116833	23.176956611798367
34	22.870392559790844	27.046209950507944	26.975612862145816	23.1077846275554
35	22.966614622348143	27.111673021504956	27.039732329397125	22.88198002674978
36	22.858314072939987	26.9645601215018	27.042227784182188	23.134898021376028
37	22.9068474542498	27.056917972975935	26.89931482126284	23.13691975151142
38	22.981169219029464	27.11264249704584	27.04107240804613	22.86511587587857
39	22.837561770796153	26.94329215138705	27.10010798315888	23.119038094657917
40	22.845822084624302	27.02208893799824	27.01455798354911	23.11753099382835
41	22.95436492648944	27.123610841112512	27.080230065057524	22.841794167340527
42	22.863837889743614	26.950500102224538	27.08482845198065	23.1008335560512
43	22.898924052602947	27.010403359075884	26.96940586354296	23.121266724778206
44	22.94640671025492	27.062275988119254	27.074689402750547	22.916627898875277
45	22.848914848469683	26.907407727777404	27.102449553567844	23.141227870185073
46	22.88358282014534	26.98938622650755	26.961492907281848	23.165538046065265
47	23.002124378484588	27.07547293428642	27.014455715830803	22.907946971398186
48	22.904273205112936	26.8989293038085	27.005904671533266	23.190892819545294
49	22.95618460101153	26.942952131374998	26.91329971868191	23.187563548931568
50	23.001469709401505	27.025807310169142	27.001576520576037	22.971146459853315
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	22087.0
1	12263.5
2	2440.0
3	1830.0
4	1220.0
5	1258.5
6	1297.0
7	1697.5
8	2098.0
9	3453.5
10	4809.0
11	7872.5
12	10936.0
13	17164.5
14	23393.0
15	35776.5
16	48160.0
17	72665.0
18	97170.0
19	137544.5
20	177919.0
21	238615.0
22	299311.0
23	447376.0
24	595441.0
25	605216.0
26	614991.0
27	720488.0
28	825985.0
29	930065.5
30	1034146.0
31	1172298.5
32	1310451.0
33	1451706.0
34	1592961.0
35	1748528.0
36	1904095.0
37	2032656.5
38	2161218.0
39	2273810.0
40	2386402.0
41	2462343.5
42	2538285.0
43	2591009.5
44	2643734.0
45	2658660.0
46	2673586.0
47	2622427.0
48	2571268.0
49	2527344.0
50	2483420.0
51	2421290.5
52	2359161.0
53	2268281.5
54	2177402.0
55	2044821.0
56	1912240.0
57	1800836.0
58	1689432.0
59	1560397.5
60	1431363.0
61	1265872.5
62	1100382.0
63	949027.0
64	797672.0
65	678858.0
66	560044.0
67	464493.0
68	368942.0
69	296952.0
70	224962.0
71	179598.5
72	134235.0
73	102611.0
74	70987.0
75	51755.5
76	32524.0
77	23829.0
78	15134.0
79	10642.5
80	6151.0
81	4343.0
82	2535.0
83	1849.0
84	1163.0
85	885.0
86	607.0
87	430.5
88	254.0
89	164.0
90	74.0
91	55.5
92	37.0
93	36.5
94	36.0
95	29.5
96	23.0
97	20.0
98	17.0
99	32.5
100	48.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.07362463356010683
2	0.0017526615270338532
3	0.002947658022738753
4	0.02293879294766008
5	0.0
6	2.3128964432998062E-5
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	9.251585773199225E-5
15	5.653746861399526E-5
16	6.142024999429486E-4
17	0.0
18	4.368804392899634E-5
19	0.0
20	0.0
21	0.0
22	3.5978389117996986E-5
23	0.0
24	0.01098368822073708
25	0.0012438243095078958
26	0.023342264882769043
27	0.024688884589756935
28	0.03960192687916668
29	0.021790054380821173
30	0.035281950300070045
31	0.07302328048484888
32	0.10362804019957933
33	0.10095535986509954
34	0.10889373443549188
35	0.0705176426712741
36	0.11989027208091395
37	0.1254412235448335
38	0.1527154123812122
39	0.1467789781767427
40	0.15231965010091425
41	0.13883032406660237
42	0.1706146609674157
43	0.18207891767137174
44	0.1519290275904903
45	0.1754511844188493
46	0.16507912881311818
47	0.17340298612406047
48	0.20544688140351078
49	0.202977221979054
50	0.19880629872630334
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	3.8912248E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.158515890203965
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.55654851763583	34.571801127328285
2	11.452833012872905	10.343858831993472
3	4.201748504581679	5.692341798323774
4	2.089734720395849	3.7747727430922757
5	1.2435236320230474	2.8077840848278464
6	0.774879568417785	2.0995446782033365
7	0.5561072489350279	1.757908462638305
8	0.42617562183313445	1.539636687245533
9	0.33470478570684903	1.360329424548305
>10	2.009132598302207	17.591197335504578
>50	0.2264604239803872	7.126874938884551
>100	0.12499842274463857	9.908999772554107
>500	0.002910182544942143	0.8500544417528826
>1k	2.199859772417773E-4	0.1264589259892806
>5k	5.693514569998046E-6	0.0136808586538772
>10k+	1.7080533743242504E-5	0.4347558884595401
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	133236	0.3424011894661033	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.48062029209929E-5	5.13976987399957E-6	0.0	0.0	0.0
2	8.48062029209929E-5	7.709654810999353E-6	0.0	0.0	0.0
3	8.994597279499246E-5	1.5419309621998707E-5	0.0	0.0	0.0
4	9.508574266899203E-5	1.7989194558998493E-5	0.0	0.0	0.0
5	1.1050505229099074E-4	2.3128964432998062E-5	0.0	0.0	0.0
6	1.1050505229099074E-4	3.34085041809972E-5	0.0	2.569884936999785E-6	0.0
7	1.1050505229099074E-4	3.34085041809972E-5	0.0	2.569884936999785E-6	0.0
8	1.182147071019901E-4	3.854827405499677E-5	0.0	2.569884936999785E-6	0.0
9	1.336340167239888E-4	3.854827405499677E-5	2.569884936999785E-6	2.569884936999785E-6	0.0
10	3.623537761169697E-4	4.368804392899634E-5	2.569884936999785E-6	5.13976987399957E-6	2.569884936999785E-6
11	4.3174066941596386E-4	4.368804392899634E-5	2.569884936999785E-6	7.709654810999353E-6	2.569884936999785E-6
12	4.574395187859617E-4	5.396758367699548E-5	2.569884936999785E-6	7.709654810999353E-6	2.569884936999785E-6
13	4.7799859828196E-4	5.396758367699548E-5	2.569884936999785E-6	1.027953974799914E-5	2.569884936999785E-6
14	5.088372175259574E-4	5.396758367699548E-5	2.569884936999785E-6	1.2849424684998924E-5	2.569884936999785E-6
15	7.118581275489404E-4	5.396758367699548E-5	2.569884936999785E-6	1.7989194558998493E-5	2.569884936999785E-6
16	7.889546756589339E-4	5.910735355099505E-5	2.569884936999785E-6	1.7989194558998493E-5	2.569884936999785E-6
17	9.405778869419212E-4	6.167723848799483E-5	2.569884936999785E-6	2.055907949599828E-5	2.569884936999785E-6
18	0.0010922010982249086	6.167723848799483E-5	2.569884936999785E-6	2.3128964432998062E-5	2.569884936999785E-6
19	0.0013157810877438899	6.167723848799483E-5	2.569884936999785E-6	2.3128964432998062E-5	2.569884936999785E-6
20	0.004456180480757627	7.195677823599397E-5	2.569884936999785E-6	2.5698849369997848E-5	2.569884936999785E-6
21	0.004800545062315598	7.452666317299376E-5	2.569884936999785E-6	3.0838619243997414E-5	2.569884936999785E-6
22	0.005085802290322574	7.709654810999354E-5	2.569884936999785E-6	3.0838619243997414E-5	2.569884936999785E-6
23	0.005422457217069546	8.223631798399312E-5	2.569884936999785E-6	3.34085041809972E-5	2.569884936999785E-6
24	0.005771961568501517	8.223631798399312E-5	2.569884936999785E-6	3.34085041809972E-5	2.569884936999785E-6
25	0.006172863618673483	8.48062029209929E-5	2.569884936999785E-6	3.34085041809972E-5	2.569884936999785E-6
26	0.006658571871766442	8.737608785799268E-5	2.569884936999785E-6	3.5978389117996986E-5	2.569884936999785E-6
27	0.007146850009796401	8.994597279499246E-5	2.569884936999785E-6	3.854827405499677E-5	2.569884936999785E-6
28	0.007835579172912343	9.251585773199225E-5	2.569884936999785E-6	3.854827405499677E-5	2.569884936999785E-6
29	0.008426652708422295	9.251585773199225E-5	2.569884936999785E-6	3.854827405499677E-5	2.569884936999785E-6
30	0.009231026693703227	9.765562760599182E-5	2.569884936999785E-6	3.854827405499677E-5	2.569884936999785E-6
31	0.016390726128184627	9.765562760599182E-5	2.569884936999785E-6	3.854827405499677E-5	2.569884936999785E-6
32	0.017241358042331557	1.0279539747999139E-4	2.569884936999785E-6	3.854827405499677E-5	2.569884936999785E-6
33	0.018302720521312467	1.0536528241699118E-4	2.569884936999785E-6	3.854827405499677E-5	2.569884936999785E-6
34	0.019284416567246384	1.0793516735399097E-4	2.569884936999785E-6	4.111815899199656E-5	2.569884936999785E-6
35	0.020466563638266287	1.182147071019901E-4	2.569884936999785E-6	4.111815899199656E-5	2.569884936999785E-6
36	0.021625581744853187	1.182147071019901E-4	2.569884936999785E-6	4.111815899199656E-5	2.569884936999785E-6
37	0.022848846974865087	1.2078459203898988E-4	2.569884936999785E-6	4.111815899199656E-5	2.569884936999785E-6
38	0.02436250920275796	1.2078459203898988E-4	2.569884936999785E-6	4.111815899199656E-5	2.569884936999785E-6
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCAACGC	42825	0.0	30.555265	12
CAACGCA	43250	0.0	30.173695	13
ATCAACG	44140	0.0	29.749554	11
AACGCAG	44865	0.0	29.087538	14
ACGCAGA	45515	0.0	28.575514	15
AAGCAGT	48860	0.0	28.00068	1
CGCAGAG	46220	0.0	27.977951	16
GTATCAA	47635	0.0	27.70507	9
GGTATCA	48190	0.0	27.436169	8
GTGGTAT	48395	0.0	27.35625	6
AGTGGTA	49690	0.0	26.753893	5
TGGTATC	49925	0.0	26.51349	7
TATCAAC	50180	0.0	26.39648	10
AGAGTAC	47960	0.0	26.17297	19
CAGAGTA	50135	0.0	25.343964	18
GAGTACT	30910	0.0	25.322807	20
GCAGAGT	51250	0.0	25.000252	17
AGCAGTG	55670	0.0	24.522118	2
GTACTTT	32850	0.0	23.424026	22
CAGTGGT	59000	0.0	22.749458	4
>>END_MODULE