FastQCFastQC Report
Thu 26 May 2016
SRR522097_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR522097_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34367936
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAG2054340.5977490181545961Illumina Paired End PCR Primer 2 (97% over 36bp)
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT403430.11738557706811373No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGA314800.036.7255425
AGATCGG291050.035.46908631
TGCCGAG327000.035.4178126
AATGCCG328800.035.30087324
CGAGATC309150.033.64788429
GAGATCG315950.032.8758130
CCGAGAT318500.032.75578328
GCCGAGA369800.031.38620827
TATGCCG34700.029.6855943
GTTCAGC417250.027.99093214
GGAATGC426450.027.57662222
GAATGCC425700.027.4974223
TCGTATG37200.026.97794340
GACCGAT38450.026.72846232
CAGGAAT449250.026.25037820
CGTATGC38800.025.97995641
AGGAATG462400.025.49277321
CTCGTAT39800.025.15869139
ACCGATC43450.023.8553533
GCAGGAA500950.023.80282219
Produced by FastQC (version 0.11.5)