FastQCFastQC Report
Thu 26 May 2016
SRR522096_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR522096_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34173080
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1049640.30715405225399645No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC337200.027.2450212
CAACGCA342550.026.64626513
ATCAACG351050.026.13881711
AACGCAG352100.025.87360214
AAGCAGT364050.025.8506151
ACGCAGA359000.025.34570115
GTATCAA370750.024.7439889
CGCAGAG375650.024.2108116
GTGGTAT379500.024.1329336
GGTATCA379950.024.1159218
AGTGGTA387350.023.6552055
TGGTATC396350.023.1624247
AGAGTAC386500.023.044419
TATCAAC401100.022.96483810
GAGTACT229200.022.2145120
CAGAGTA407550.022.06420518
GCAGAGT420600.021.56066517
AGCAGTG447750.020.939782
GTACTTT251150.020.33931222
AGTACTT262450.019.5189221
Produced by FastQC (version 0.11.5)