##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR522096_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	34173080
Sequences flagged as poor quality	0
Sequence length	50
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.95188709943616	40.0	39.0	40.0	38.0	40.0
2	38.8355817795762	40.0	39.0	40.0	37.0	40.0
3	38.81683632262588	40.0	39.0	40.0	37.0	40.0
4	38.809698481963	40.0	39.0	40.0	37.0	40.0
5	38.81719637211513	40.0	39.0	40.0	37.0	40.0
6	38.85261931321379	40.0	39.0	40.0	37.0	40.0
7	38.82896750892808	40.0	39.0	40.0	37.0	40.0
8	38.80363376669589	40.0	39.0	40.0	36.0	40.0
9	38.79688956921647	40.0	39.0	40.0	36.0	40.0
10	38.78836923683789	40.0	39.0	40.0	36.0	40.0
11	38.822579703087925	40.0	39.0	40.0	37.0	40.0
12	38.78685874963568	40.0	39.0	40.0	36.0	40.0
13	38.766422166219726	40.0	39.0	40.0	36.0	40.0
14	38.743218433925186	40.0	39.0	40.0	36.0	40.0
15	38.730032528528305	40.0	39.0	40.0	36.0	40.0
16	38.70595132191772	40.0	39.0	40.0	36.0	40.0
17	38.6457779339761	40.0	39.0	40.0	36.0	40.0
18	38.61740618638999	40.0	39.0	40.0	36.0	40.0
19	38.45967545213952	40.0	39.0	40.0	35.0	40.0
20	38.43204133194901	40.0	39.0	40.0	35.0	40.0
21	38.502257390905356	40.0	39.0	40.0	35.0	40.0
22	38.467693254456435	40.0	38.0	40.0	35.0	40.0
23	38.406120841317204	40.0	38.0	40.0	35.0	40.0
24	38.36995023568259	40.0	38.0	40.0	35.0	40.0
25	38.33991823388468	40.0	38.0	40.0	35.0	40.0
26	38.28757668316698	40.0	38.0	40.0	35.0	40.0
27	38.23209927229269	40.0	38.0	40.0	35.0	40.0
28	38.183255211412025	40.0	38.0	40.0	35.0	40.0
29	38.1261789104172	40.0	38.0	40.0	35.0	40.0
30	38.0699476605562	40.0	38.0	40.0	35.0	40.0
31	37.99962145642125	40.0	38.0	40.0	35.0	40.0
32	37.9386798321954	40.0	38.0	40.0	35.0	40.0
33	37.874915840187654	40.0	38.0	40.0	35.0	40.0
34	37.81667701594355	40.0	38.0	40.0	34.0	40.0
35	37.73464990571526	40.0	38.0	40.0	33.0	40.0
36	37.62601260992571	39.0	38.0	40.0	33.0	40.0
37	37.53808805059421	39.0	38.0	40.0	33.0	40.0
38	37.46472676738532	39.0	38.0	40.0	33.0	40.0
39	37.60053170507312	40.0	38.0	40.0	33.0	40.0
40	37.709088059958304	40.0	38.0	40.0	34.0	40.0
41	37.86448681242663	40.0	38.0	40.0	35.0	40.0
42	37.79621807574851	40.0	38.0	40.0	35.0	40.0
43	37.76547700704765	40.0	38.0	40.0	34.0	40.0
44	37.69367142206673	40.0	38.0	40.0	34.0	40.0
45	37.63659011713314	40.0	38.0	40.0	34.0	40.0
46	37.56597210435817	40.0	38.0	40.0	34.0	40.0
47	37.49837058877924	40.0	38.0	40.0	33.0	40.0
48	37.36741961801511	40.0	38.0	40.0	33.0	40.0
49	37.282489316151775	39.0	38.0	40.0	33.0	40.0
50	37.18908362957041	39.0	38.0	40.0	33.0	40.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	-0.19084795136357258
1	2	-0.21369903496754006
1	3	-0.21153809419279668
1	4	-0.22112490897610826
1	5	-0.2322836990983248
1	6	-0.20231553662490143
1	7	-0.2005303773483078
1	8	-0.21309548032434833
1	9	-0.2164743935956679
1	10	-0.21900590280894505
1	11	-0.23037070253491265
1	12	-0.23862816837930012
1	13	-0.23261670557124603
1	14	-0.2365604214628263
1	15	-0.2692601536363952
1	16	-0.19016651865563716
1	17	-0.20064040583796583
1	18	-0.21357022089745215
1	19	-0.05916187279973428
1	20	-0.10520214622987822
1	21	-0.21190532712917332
1	22	-0.15475762147431027
1	23	-0.1635018479462218
1	24	-0.16105889571120002
1	25	-0.15380737126214683
1	26	-0.22287426843355718
1	27	-0.21982390444704691
1	28	-0.23324280279910425
1	29	-0.23364884154238297
1	30	-0.2731900741294879
1	31	-0.27940358814069555
1	32	-0.22836686962454422
1	33	-0.21167794290441577
1	34	-0.18082338418519583
1	35	-0.27890585271712354
1	36	-0.17751745312249767
1	37	-0.21619246293047212
1	38	-0.2569079691830254
1	39	-0.09344191455606676
1	40	-0.19994168832277381
1	41	-0.12027145234980452
1	42	-0.2334873089117835
1	43	-0.13634546833661432
1	44	-0.10583977420559876
1	45	-0.12775854368477724
1	46	-0.13848688965214961
1	47	-0.132546671625839
1	48	-0.13894517335614864
1	49	-0.12390660434439837
1	50	-0.14197199882648448
2	1	-0.15665210682578845
2	2	-0.17411615402760106
2	3	-0.15840734953253843
2	4	-0.18274242460250179
2	5	-0.17540282341563085
2	6	-0.18569061655312424
2	7	-0.1933868311973228
2	8	-0.21331133397351465
2	9	-0.2248279883018114
2	10	-0.20767223096137855
2	11	-0.2185294053295479
2	12	-0.20653629539626195
2	13	-0.2180353784920186
2	14	-0.23555681758129055
2	15	-0.23307846068919957
2	16	-0.18091591833095322
2	17	-0.20943018890042708
2	18	-0.184017107606131
2	19	-0.14514951403824483
2	20	-0.09536160224816825
2	21	-0.1624878039585127
2	22	-0.17733717684610895
2	23	-0.1539584041620472
2	24	-0.178556955361465
2	25	-0.15681791585920735
2	26	-0.2452874353749479
2	27	-0.25554204289456806
2	28	-0.2765951721486317
2	29	-0.31463353731757593
2	30	-0.30526468248804406
2	31	-0.23101061873231288
2	32	-0.2714391051979206
2	33	-0.23598911029127123
2	34	-0.2742212132181763
2	35	-0.27056161268686196
2	36	-0.19708307773422717
2	37	-0.2598916318079816
2	38	-0.21162870840313275
2	39	-0.23256841068911172
2	40	-0.2692437015686622
2	41	-0.2508628646971687
2	42	-0.3140421426872493
2	43	-0.2483990277575856
2	44	-0.23325073027807264
2	45	-0.22540183155805238
2	46	-0.2938982296962891
2	47	-0.26733162965881974
2	48	-0.4478524993586248
2	49	-0.48414680226238715
2	50	-0.40193068642717833
3	1	-0.1459962924605378
3	2	-0.15684513727239136
3	3	-0.14289716833673083
3	4	-0.22044797125002447
3	5	-0.16231448827209505
3	6	-0.151799000881212
3	7	-0.1590545286358349
3	8	-0.14531418873220048
3	9	-0.18516512024788057
3	10	-0.17877758116364362
3	11	-0.1956922711287632
3	12	-0.19430387558594475
3	13	-0.17603889592066224
3	14	-0.18743345353536256
3	15	-0.2151551555833393
3	16	-0.169623766375814
3	17	-0.1920194059264091
3	18	-0.19882652045615856
3	19	-0.002024470499684128
3	20	-0.019298816749298453
3	21	-0.13342037439465315
3	22	-0.14436531923346507
3	23	-0.1289637685816416
3	24	-0.1267436462544822
3	25	-0.11947220470185727
3	26	-0.24551352112968772
3	27	-0.20824701900883724
3	28	-0.2046492693440527
3	29	-0.1468297700231389
3	30	-0.16363118028432666
3	31	-0.20291451618336964
3	32	-0.18637003099031801
3	33	-0.1831935297793592
3	34	-0.17333898174516804
3	35	-0.22314341819176775
3	36	-0.12974933478820105
3	37	-0.19030574984923732
3	38	-0.20757347955688488
3	39	-0.10633387357448498
3	40	-0.14798057808610565
3	41	-0.14802978555747615
3	42	-0.13264386739685108
3	43	-0.14015796881466258
3	44	-0.1856874736879277
3	45	-0.1456316476948203
3	46	-0.24399135937552074
3	47	-0.193379151634268
3	48	-0.4518654622062144
3	49	-0.5013029475657902
3	50	-0.5859499235172478
4	1	-0.12446495998871399
4	2	-0.1495255823190078
4	3	-0.12182904168917474
4	4	-0.15068498083207515
4	5	-0.157968340209095
4	6	-0.1673194690601818
4	7	-0.14298477043760727
4	8	-0.15435659162555737
4	9	-0.16552323132071223
4	10	-0.2055251785379184
4	11	-0.17416579749760075
4	12	-0.1944871922854503
4	13	-0.1879641169021653
4	14	-0.18579697920272054
4	15	-0.18892728708557627
4	16	-0.13908235480874254
4	17	-0.1967732994616398
4	18	-0.14460837915288494
4	19	-0.12786012364717436
4	20	-0.08707089857668393
4	21	-0.1329749806675835
4	22	-0.1649631517367851
4	23	-0.2741884884328911
4	24	-0.15890402316393448
4	25	-0.13748949068027372
4	26	-0.13321224167560786
4	27	-0.20113837078966412
4	28	-0.21057641951901473
4	29	-0.18443982243311297
4	30	-0.16969993343656142
4	31	-0.12398564422864666
4	32	-0.15850718999998037
4	33	-0.09908229251114165
4	34	-0.157444009429625
4	35	-0.1196034355136888
4	36	-0.16269971518352122
4	37	-0.10437565825885997
4	38	-0.15808604228249834
4	39	-0.044571712939159625
4	40	-0.11647810755690102
4	41	-0.13521019370401888
4	42	-0.09505288150855051
4	43	-0.16411952594491197
4	44	-0.20920719038081614
4	45	-0.14244511919847724
4	46	-0.15169608883380192
4	47	-0.16581378453568618
4	48	-0.41865563140575546
4	49	-0.39172711618421374
4	50	-0.4305891215715292
5	1	-0.10705263818667987
5	2	-0.16488200416574728
5	3	-0.1460551724086514
5	4	-0.13576903097931847
5	5	-0.14189214381786996
5	6	-0.15108992439959223
5	7	-0.12943328307381563
5	8	-0.1465433884760401
5	9	-0.15466895707416484
5	10	-0.1525935155330771
5	11	-0.21344475555122955
5	12	-0.15187446778290337
5	13	-0.1693790387513232
5	14	-0.16445420351015372
5	15	-0.16876633157556853
5	16	-0.15238230992163437
5	17	-0.13307115205080322
5	18	-0.11992806372714426
5	19	0.01401392762112863
5	20	0.02945027176841819
5	21	-0.06356791685810492
5	22	-0.05966750205865168
5	23	-0.06326716030847024
5	24	-0.10207555793576972
5	25	-0.10156250071092643
5	26	-0.07202113218682626
5	27	-0.10317726359770063
5	28	-0.09674412981437541
5	29	-0.13243907978493752
5	30	-0.05031554673261951
5	31	-0.04237263642096423
5	32	-0.05110909980384548
5	33	-0.04156422699278295
5	34	-0.051674000317831315
5	35	-0.010766810512549796
5	36	-0.014486093309102444
5	37	-0.001690143062489824
5	38	-0.030790151023566636
5	39	-0.001126754118168094
5	40	0.02762899571044386
5	41	0.0282730986893327
5	42	0.026013718716264123
5	43	0.05272785189745832
5	44	0.020884797065406246
5	45	0.053167546262010035
5	46	-0.08693593045427406
5	47	-0.047623377402025824
5	48	-0.2416613194519357
5	49	-0.30809897024671784
5	50	-0.15385952007081727
6	1	-0.12310519497627581
6	2	-0.12617915900731447
6	3	-0.17415653391457653
6	4	-0.16503216785457653
6	5	-0.1404149410861777
6	6	-0.12451090528183784
6	7	-0.13012970987568906
6	8	-0.12899917529652782
6	9	-0.1439412851009294
6	10	-0.1272143228709126
6	11	-0.09516375826940049
6	12	-0.12658605235250775
6	13	-0.1252745685579697
6	14	-0.11157582282231004
6	15	-0.11746787249499846
6	16	-0.09913990480873025
6	17	-0.08424791463619385
6	18	-0.09223401382057972
6	19	0.023469065782329324
6	20	-0.019433212888024798
6	21	-0.06722782469793032
6	22	-0.09459182820375389
6	23	-0.08879052055105774
6	24	-0.05636594229688541
6	25	-0.08485145330136845
6	26	-0.13347711070419166
6	27	-0.0951783567247304
6	28	-0.10506742658829182
6	29	-0.050826507072287086
6	30	-0.10153098042060549
6	31	-0.11770842779854718
6	32	-0.11336160081663138
6	33	-0.0853132943233561
6	34	-0.14534717365827987
6	35	-0.0520010347315889
6	36	-0.0985241565285051
6	37	-0.1333953042449565
6	38	-0.12333723286467801
6	39	-0.10013902304597622
6	40	-0.057307298095423675
6	41	-0.0276097558606736
6	42	-0.06536383591632955
6	43	-0.11113167011731662
6	44	-0.10540669841508077
6	45	-0.03606155036109726
6	46	-0.054535279173855145
6	47	-0.0626207153160081
6	48	-0.22691536794687295
6	49	-0.22965727156532978
6	50	-0.32795782131168494
7	1	-0.1216476258349104
7	2	-0.13338112278574954
7	3	-0.12083110275299447
7	4	-0.12284187093073484
7	5	-0.1198230151241404
7	6	-0.13791118754576814
7	7	-0.16252554473019387
7	8	-0.172785663228197
7	9	-0.15911483912120872
7	10	-0.14573440435872698
7	11	-0.17129050739463025
7	12	-0.1850379111360283
7	13	-0.1671821517850276
7	14	-0.16671396825200446
7	15	-0.16061186219695855
7	16	-0.1720649543654531
7	17	-0.13006195724849334
7	18	-0.1415721465424582
7	19	-0.03196957143528323
7	20	-0.06503220248772124
7	21	-0.1121385824835599
7	22	-0.11957984570890545
7	23	-0.08533163441362035
7	24	-0.07011391640119768
7	25	-0.12667332279034582
7	26	-0.1500677567003521
7	27	-0.17964233043405642
7	28	-0.14288368946354524
7	29	-0.0892120808358996
7	30	-0.17194525232256552
7	31	-0.10108772865505244
7	32	-0.1748749565024852
7	33	-0.11953841018910083
7	34	-0.15810462981593076
7	35	-0.13202994777554977
7	36	-0.1896485638166041
7	37	-0.14557684851799024
7	38	-0.20190538871096209
7	39	-0.06695694112623585
7	40	-0.1236672647368664
7	41	-0.10780206671199721
7	42	-0.14325690335795827
7	43	-0.287393586121496
7	44	-0.10177099704299764
7	45	-0.11334188169444559
7	46	-0.04947408765028172
7	47	-0.13360211824683432
7	48	-0.08042459329506357
7	49	-0.14243207069262098
7	50	-0.08139698548458085
8	1	-0.08908796204066505
8	2	-0.11681365002188926
8	3	-0.10586916285325998
8	4	-0.12039651152512931
8	5	-0.13066544050812468
8	6	-0.10206912537477564
8	7	-0.11346379698894538
8	8	-0.11367475583603692
8	9	-0.10981277156809455
8	10	-0.10930597971923817
8	11	-0.1304373807319763
8	12	-0.14673891247302606
8	13	-0.152826401733833
8	14	-0.16115547200768532
8	15	-0.15856729843594763
8	16	-0.10436268276905025
8	17	-0.08051425863789774
8	18	-0.1287674364517244
8	19	0.03323488851340528
8	20	7.997437372040395E-4
8	21	-0.061550172208342246
8	22	-0.050738130440848295
8	23	-0.06525654359441546
8	24	-0.08922235099171871
8	25	-0.06328462385774714
8	26	-0.0911472453166624
8	27	-0.0734997981897223
8	28	-0.10885693006484587
8	29	-0.04762284315912524
8	30	-0.08001033639300914
8	31	-0.13755889381687325
8	32	-0.07985506333921677
8	33	-0.0960307780339491
8	34	-0.08249687827064633
8	35	-0.034140046114956135
8	36	-0.024568651863056346
8	37	-0.04233462506994812
8	38	-0.03708779322398925
8	39	-0.02139198888424687
8	40	-0.057296716288306015
8	41	-0.009614231324107436
8	42	0.008862109776217153
8	43	-0.06052962019257535
8	44	-0.03947074048649313
8	45	-0.00403684041540231
8	46	0.008545351622991859
8	47	-0.02609193692242684
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114	28	-0.17768733189114982
114	29	-0.06810220382641319
114	30	-0.09684032008503607
114	31	-0.10035086594186282
114	32	-0.10603561472209577
114	33	-0.08623599003937699
114	34	-0.08468665169174727
114	35	-0.16540737382125315
114	36	-0.05933498878200538
114	37	-0.03681434903501213
114	38	-0.04129152732794239
114	39	-0.086368497883484
114	40	0.043649787384303806
114	41	0.022274075207825206
114	42	0.13213871606249228
114	43	-0.017161163627143594
114	44	0.17688794135726482
114	45	-0.006782749243761543
114	46	0.09689267462058382
114	47	0.21771076421838131
114	48	0.2008163487291128
114	49	0.07705925555090687
114	50	0.2167967365950929
115	1	-0.10082310236637682
115	2	-0.11082199991449215
115	3	-0.1189330870105536
115	4	-0.09954114422754401
115	5	-0.10484069698024712
115	6	-0.1364156912732355
115	7	-0.1408149859506551
115	8	-0.1250683203903762
115	9	-0.12085755021061573
115	10	-0.10866813875068715
115	11	-0.13907171795842999
115	12	-0.12159850039998332
115	13	-0.10060355736747795
115	14	-0.11917359556646545
115	15	-0.13619754811150386
115	16	-0.10755783397866026
115	17	-0.10987321720170229
115	18	-0.11219649959521405
115	19	0.0030774044582315696
115	20	-0.04311534356415336
115	21	-0.13682195354724058
115	22	-0.05181809324253095
115	23	0.01301479073527645
115	24	-0.16459043070886992
115	25	-0.060852680738044285
115	26	-0.08429904339697458
115	27	-0.19150144416902748
115	28	-0.09258326864429023
115	29	-0.01033019671524471
115	30	-0.011125668785325615
115	31	-0.02007139891516374
115	32	-0.03984227488649594
115	33	0.00254435272546516
115	34	-0.034904514031310896
115	35	-0.018889818980902362
115	36	0.020810548359690983
115	37	0.037294511127456076
115	38	0.05056834922239517
115	39	-0.020135257700353293
115	40	0.12196755442847262
115	41	0.03163971347303374
115	42	0.08598156322502604
115	43	0.03514752362791285
115	44	0.19966584993517245
115	45	0.12683683506112686
115	46	0.15041548395420534
115	47	0.11394642773362307
115	48	0.1486573582370525
115	49	0.05799523589020339
115	50	-0.12454673041189324
116	1	-0.11694165143273239
116	2	-0.1716062242487979
116	3	-0.1929265687039745
116	4	-0.14553510760616462
116	5	-0.15110192185844085
116	6	-0.14711781258382928
116	7	-0.1709693962454537
116	8	-0.1506836764792041
116	9	-0.13934087143143614
116	10	-0.14319649798873257
116	11	-0.1475501260787624
116	12	-0.1173013149446902
116	13	-0.13301157560947985
116	14	-0.13935601258239672
116	15	-0.14200474502432314
116	16	-0.17690509311227487
116	17	-0.20366101790587976
116	18	-0.09233400590761676
116	19	0.017686643961155823
116	20	-0.021451376401948608
116	21	-0.11170415139901735
116	22	-0.08782336814866198
116	23	-0.1669445361034434
116	24	-0.08358458412482861
116	25	-0.0516021930679571
116	26	-0.08542710385986396
116	27	-0.049729660063299264
116	28	-0.10047952382537062
116	29	-0.12783818409209857
116	30	-0.1802262669121859
116	31	-0.007354981931804616
116	32	-0.023714915566380057
116	33	0.01735426473863555
116	34	0.02663069150941766
116	35	0.0091319582739402
116	36	0.01380749196877673
116	37	0.023798382731783363
116	38	0.029249553804262973
116	39	0.13722336993480155
116	40	0.12373438003796622
116	41	0.13907146344638477
116	42	0.16594686861969876
116	43	0.18120732704520748
116	44	0.21342436018358768
116	45	0.20439152967404794
116	46	0.20082397168624766
116	47	0.2009224016101001
116	48	0.07912296425197951
116	49	0.25668910074345774
116	50	0.13564281444731563
117	1	-0.12993094498774127
117	2	-0.1469333476383241
117	3	-0.14433409412826137
117	4	-0.18333414758443922
117	5	-0.20292499527430152
117	6	-0.16528349845652457
117	7	-0.1832622021754986
117	8	-0.18600782573101782
117	9	-0.1941811426914839
117	10	-0.1840422318998023
117	11	-0.19773281069045368
117	12	-0.1749773086100177
117	13	-0.19074066633221065
117	14	-0.19600961860231791
117	15	-0.195973431306939
117	16	-0.1810953173539076
117	17	-0.17682556020142925
117	18	-0.20043157410979973
117	19	-0.012457301927284448
117	20	-0.0032024036675579737
117	21	-0.17165245324711265
117	22	-0.08650810369127981
117	23	-0.12227321482110654
117	24	-0.1492816434944615
117	25	-0.11500379698195218
117	26	-0.1118722337968805
117	27	-0.15235002092085637
117	28	-0.16066162542317386
117	29	-0.1464646845149744
117	30	-0.13857719267050328
117	31	-0.13099159716849584
117	32	-0.14549714973471595
117	33	-0.1683336646300333
117	34	-0.14249502750567444
117	35	-0.18737106894033673
117	36	-0.04783396790899985
117	37	-0.057000378372784155
117	38	-0.016624389484533708
117	39	-0.06135471896791955
117	40	-0.01680722306078053
117	41	-0.03090899525587787
117	42	-0.042805111155601594
117	43	-0.13050603485387313
117	44	-0.038299975536666864
117	45	-0.019104130144967257
117	46	0.030913297269847817
117	47	-0.1332539631287304
117	48	-0.10246839834724142
117	49	-0.1327304933316995
117	50	-0.1485506089652091
118	1	-0.12260176204957673
118	2	-0.1618659151852384
118	3	-0.15688164420055983
118	4	-0.15596393932558783
118	5	-0.2065447764134234
118	6	-0.1898602883628513
118	7	-0.1583421490520891
118	8	-0.1595406742119252
118	9	-0.20406040995455754
118	10	-0.1638641013978912
118	11	-0.2194792283774447
118	12	-0.1672062042063729
118	13	-0.1922837925542069
118	14	-0.19628409358318066
118	15	-0.1793004944390333
118	16	-0.11991762686222529
118	17	-0.1690189215991893
118	18	-0.18331052718195906
118	19	-3.1391207773623364E-4
118	20	-0.06577965619256787
118	21	-0.16038985935356465
118	22	-0.15384607930101168
118	23	-0.12305402460776804
118	24	-0.18421008994739196
118	25	-0.19877967324777046
118	26	-0.1301155834891361
118	27	-0.16770767382725893
118	28	-0.13072329725864051
118	29	-0.08160722659082609
118	30	-0.11715768111189107
118	31	-0.05650244569829255
118	32	-0.11445029538192841
118	33	-0.0434665747882832
118	34	-0.07358906892546457
118	35	-0.0774754542461693
118	36	-0.027824872568032788
118	37	-0.023556607407989816
118	38	-0.02245201254052631
118	39	0.07579135835216988
118	40	0.047803871244489926
118	41	0.08169267894241727
118	42	0.04957598542721797
118	43	0.06867156970442068
118	44	0.1002528558365654
118	45	0.05373087801537224
118	46	-0.005186058682703276
118	47	-0.03801365864408979
118	48	-0.06838340478196159
118	49	-0.0858983742830759
118	50	0.0013969381576615092
119	1	-0.09669322634584887
119	2	-0.12266638535774632
119	3	-0.15033964672304023
119	4	-0.1133721575428055
119	5	-0.15382700026192708
119	6	-0.1433536803629636
119	7	-0.1150864204349844
119	8	-0.14296303560427503
119	9	-0.19227870861561058
119	10	-0.17435638383405205
119	11	-0.147676238663891
119	12	-0.1333429728415183
119	13	-0.20427583352288536
119	14	-0.17610288722615763
119	15	-0.18785076927639466
119	16	-0.11947772306407956
119	17	-0.09967658054607398
119	18	-0.13252944871388195
119	19	0.031841772852686745
119	20	0.0018228270058742169
119	21	-0.08145273620140614
119	22	-0.06631199668004228
119	23	-0.15596578973143949
119	24	-0.06270266538508196
119	25	-0.1084793149964085
119	26	-0.11585559688313651
119	27	-0.19030908177020933
119	28	-0.14769692463766404
119	29	-0.1891903832576034
119	30	-0.13519968706187058
119	31	-0.08403260444306682
119	32	-0.06453399386272451
119	33	-0.05175447358626428
119	34	-0.10978788574387721
119	35	-0.06887872026561581
119	36	-0.011991549755897779
119	37	-0.08466449513649366
119	38	-0.014009558194310046
119	39	0.06014355053042664
119	40	-0.05118446856760528
119	41	0.03486324502249971
119	42	0.03707153048812728
119	43	-0.023349330525938683
119	44	0.07553854208099864
119	45	0.012412306621037317
119	46	0.07134119348042134
119	47	0.05915147828774536
119	48	0.016490128608417365
119	49	-0.017953505723561136
119	50	0.10573811033484759
120	1	-0.10757218007831426
120	2	-0.13167533751339988
120	3	-0.12224067430576468
120	4	-0.12652377899135558
120	5	-0.10197361266599358
120	6	-0.17441380251017335
120	7	-0.14836844778491098
120	8	-0.15584961607527958
120	9	-0.18904977110791776
120	10	-0.13813618806664607
120	11	-0.1457149129919486
120	12	-0.13006919928966454
120	13	-0.1399845877123198
120	14	-0.12760525179902515
120	15	-0.12958353026220237
120	16	-0.1038801760636261
120	17	-0.08951335805788574
120	18	-0.07095502952251564
120	19	0.037311660854825845
120	20	-0.02491605952671705
120	21	-0.07025889146168396
120	22	-0.05473723956119869
120	23	-0.1573294030760266
120	24	-0.042354944393579785
120	25	-0.11275409621725174
120	26	-0.1419455766411346
120	27	-0.03640421951040196
120	28	-0.07631969904688418
120	29	-0.0500494954722015
120	30	-0.05177849608966767
120	31	-0.022333075887942755
120	32	-0.008415609002454971
120	33	-0.021721819264463704
120	34	-0.00887124064890088
120	35	0.02450040493852157
120	36	0.0016590731078380827
120	37	0.029208418564614647
120	38	0.05913954612691441
120	39	0.20598694590106703
120	40	0.13568033581171335
120	41	0.15493141981841774
120	42	0.15950200488682498
120	43	0.17725023967798847
120	44	0.18315385503743897
120	45	0.14806571736731655
120	46	0.10681039368864731
120	47	0.1486886396379603
120	48	0.20447527126891174
120	49	0.2747420469280115
120	50	0.2769238079417349
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	21952.0
3	928.0
4	1484.0
5	1856.0
6	2675.0
7	4256.0
8	5163.0
9	6260.0
10	9177.0
11	10805.0
12	13389.0
13	18148.0
14	19504.0
15	20532.0
16	23636.0
17	24977.0
18	26760.0
19	31173.0
20	36600.0
21	36969.0
22	45283.0
23	57454.0
24	73714.0
25	83771.0
26	107604.0
27	133927.0
28	162810.0
29	193160.0
30	236695.0
31	290555.0
32	356378.0
33	453587.0
34	619546.0
35	906750.0
36	1426787.0
37	2503552.0
38	4478445.0
39	2.125528E7
40	471538.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	13.902279397418326	30.616804835934126	26.169712366916016	29.311203399731532
2	23.54589419706115	30.307486692828117	25.729737043880878	20.416882066229856
3	22.700601420194452	28.141682163699656	26.05247655996628	23.10523985613961
4	23.21448413042722	26.68200106594774	26.303107650567313	23.80040715305773
5	24.243097198145442	28.23041118915825	27.432496573326137	20.09399503937017
6	24.546672409978846	26.865172820243306	24.803813411024116	23.784341358753732
7	23.96748551784036	26.636501597163615	27.528765332243978	21.86724755275205
8	23.67259550499984	26.73514942170855	26.733165403879312	22.859089669412295
9	22.79620098627341	26.231603940879783	27.688961603695073	23.283233469151742
10	22.670490924435256	27.566233421160753	26.307663810227233	23.45561184417676
11	22.805287085624123	27.38001666809079	26.470587374623534	23.344108871661554
12	22.6696715660397	26.713085270628223	27.186416910620874	23.4308262527112
13	22.809931091958934	27.039225027419246	26.52326919317779	23.627574687444035
14	22.913123429319217	27.651832963256457	26.574616628059278	22.860426979365045
15	22.629403027178117	27.548207536458523	26.5151546187818	23.30723481758156
16	22.762721416974998	26.905043385027046	26.665445432486624	23.666789765511332
17	23.435382470646484	26.772266357027224	26.69590215456143	23.09644901776486
18	22.65235848119366	26.77389592677252	26.670469465827672	23.903276126206148
19	22.95727514171974	27.46004457309672	26.555124677085	23.027555608098538
20	23.54667182472285	26.82954243515656	26.650691128806653	22.973094611313936
21	22.9270466694837	27.237111785065903	26.644183082121952	23.191658463328444
22	23.36209477507482	26.66680400819145	26.726761192166183	23.24434002456755
23	22.862835307791983	26.83584271596239	27.282305253140777	23.019016723104855
24	22.75523337292038	27.234001420006464	26.613195618284163	23.39756958878899
25	22.74987220736364	26.79873622534673	26.642016861019552	23.809374706270074
26	22.948007562851362	26.974494753268942	27.130950684697027	22.946546999182672
27	23.003737254987588	26.629959891374156	27.208153506389067	23.158149347249193
28	23.226821717563332	26.710914505229272	26.820438210754276	23.241825566453116
29	23.30830882245527	26.784875499884464	26.920890856232198	22.985924821428064
30	23.209675822499467	26.655777186727914	26.92020642359062	23.214340567182003
31	23.035127463901055	26.832058067026576	26.87413621680645	23.25867825226592
32	23.14602352856248	26.807144234967804	26.93651956998602	23.110312666483694
33	23.022611408852985	26.78935913295441	26.806109127695315	23.38192033049729
34	23.04079682414901	26.84663069682684	26.832610766966326	23.279961712057826
35	23.148892218373934	26.87869897979362	26.809668251144004	23.16274055068844
36	23.05014477320525	26.76234308421871	26.839352290452474	23.34815985212357
37	23.083368541684592	26.857276052253614	26.736022211305947	23.32333319475584
38	23.157407724100747	26.907500082136735	26.84036923519164	23.094722958570873
39	23.051991979283066	26.74637508330005	26.877671290268236	23.323961647148643
40	23.03245303698401	26.806878961191412	26.84257742498208	23.318090576842504
41	23.118379690370865	26.891699508949387	26.88573435826836	23.104186442411397
42	23.095699426297806	26.740041003184995	26.855983934556253	23.30827563596095
43	23.0726381288554	26.78858776445224	26.821883112277657	23.316890994414702
44	23.151979420652964	26.81748212575546	26.835235881630524	23.195302571961047
45	23.054787532583354	26.712998443716263	26.874344352655687	23.357869671044696
46	23.066755799498043	26.769653202917926	26.811774329744566	23.35181666783947
47	23.190285768474176	26.84111304269904	26.797609796288334	23.170991392538447
48	23.088857123562185	26.712919437714422	26.780404873319952	23.417818565403444
49	23.083050786664536	26.76089734567679	26.75028947292246	23.405762394736218
50	23.18618088952575	26.79201630422269	26.796122570282044	23.225680235969513
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	31520.0
1	17387.0
2	3254.0
3	2385.5
4	1517.0
5	1584.0
6	1651.0
7	2049.5
8	2448.0
9	3867.0
10	5286.0
11	8524.5
12	11763.0
13	17697.0
14	23631.0
15	35552.0
16	47473.0
17	70298.0
18	93123.0
19	129500.5
20	165878.0
21	222495.0
22	279112.0
23	407134.5
24	535157.0
25	548731.0
26	562305.0
27	655256.0
28	748207.0
29	837408.5
30	926610.0
31	1039183.0
32	1151756.0
33	1259319.5
34	1366883.0
35	1485232.5
36	1603582.0
37	1702966.0
38	1802350.0
39	1885958.5
40	1969567.0
41	2023962.5
42	2078358.0
43	2121584.0
44	2164810.0
45	2183554.0
46	2202298.0
47	2178256.5
48	2154215.0
49	2137222.0
50	2120229.0
51	2085201.0
52	2050173.0
53	1998133.5
54	1946094.0
55	1854647.5
56	1763201.0
57	1680233.0
58	1597265.0
59	1490279.5
60	1383294.0
61	1236027.5
62	1088761.0
63	947114.0
64	805467.0
65	691372.5
66	577278.0
67	483210.5
68	389143.0
69	316268.5
70	243394.0
71	192447.5
72	141501.0
73	107868.5
74	74236.0
75	53988.5
76	33741.0
77	24594.0
78	15447.0
79	10765.5
80	6084.0
81	4351.5
82	2619.0
83	1957.0
84	1295.0
85	952.5
86	610.0
87	429.5
88	249.0
89	164.0
90	79.0
91	61.5
92	44.0
93	40.5
94	37.0
95	27.5
96	18.0
97	25.0
98	32.0
99	33.5
100	35.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11301293298701787
2	4.5064711755569005E-4
3	0.01693438226814791
4	0.001969386429318048
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	2.0483959888895E-5
19	0.0
20	0.0
21	0.0
22	0.0010563870742701564
23	0.0
24	0.008231625595351663
25	0.0015567809515560202
26	0.023960380510038896
27	0.021543273243149287
28	0.04441507759909262
29	0.04224670413085388
30	0.0681852499101632
31	0.09318738609455163
32	0.11883037759546403
33	0.12210195861771897
34	0.10523488078920601
35	0.1141424770608912
36	0.13838378044940639
37	0.11866943219633701
38	0.15552300231644323
39	0.14031805151891488
40	0.16611320957900194
41	0.17050555583517787
42	0.1773559772780212
43	0.19375777658905782
44	0.18139424365611762
45	0.19744781564904304
46	0.19512434934164552
47	0.20448259273088643
48	0.20749373483455397
49	0.22170375043747886
50	0.23072254534856093
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	3.417308E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.32205559900444
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.563092346742	32.88708505774734
2	14.347830429517089	13.005463369033226
3	4.930250246336943	6.70347227344465
4	2.3879994488085328	4.329161751571692
5	1.3495755837606414	3.0582769821129334
6	0.817784922751348	2.223821624217849
7	0.5935077006574484	1.8829292305343914
8	0.40577746991397645	1.4712535241811675
9	0.32247695243108293	1.3153786530731146
>10	1.9487999076076148	17.076001134794733
>50	0.2280650116843243	7.137700292306616
>100	0.10292055059689298	7.851727841143472
>500	0.0018212373715543121	0.5281708635851038
>1k	6.590246588459881E-5	0.045873700471630016
>5k	1.2915741899417415E-5	0.04358942606836409
>10k+	1.937361284089888E-5	0.4400942757137028
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	104964	0.30715405225399645	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.0827235941273072E-4	8.778839952383573E-6	0.0	2.9262799841278576E-6	0.0
2	1.0827235941273072E-4	8.778839952383573E-6	0.0	1.170511993651143E-5	0.0
3	1.1119863939685858E-4	1.7557679904767145E-5	0.0	1.170511993651143E-5	0.0
4	1.1705119936511429E-4	1.7557679904767145E-5	0.0	1.170511993651143E-5	0.0
5	1.2583003931749786E-4	1.7557679904767145E-5	0.0	1.170511993651143E-5	0.0
6	1.2583003931749786E-4	2.0483959888895003E-5	0.0	1.170511993651143E-5	0.0
7	1.3460887926988144E-4	2.341023987302286E-5	0.0	1.170511993651143E-5	0.0
8	1.4631399920639286E-4	2.341023987302286E-5	0.0	1.4631399920639286E-5	0.0
9	1.6094539912703216E-4	2.341023987302286E-5	0.0	1.4631399920639286E-5	0.0
10	7.110860361430693E-4	2.341023987302286E-5	0.0	1.4631399920639286E-5	0.0
11	8.339897954764393E-4	2.9262799841278572E-5	0.0	1.7557679904767145E-5	0.0
12	8.632525953177179E-4	2.9262799841278572E-5	0.0	1.7557679904767145E-5	0.0
13	8.866628351907408E-4	3.218907982540643E-5	0.0	1.7557679904767145E-5	0.0
14	9.100730750637636E-4	3.218907982540643E-5	0.0	2.0483959888895003E-5	0.0
15	0.0012817106330480015	3.511535980953429E-5	0.0	2.341023987302286E-5	0.0
16	0.001372425312555965	3.511535980953429E-5	0.0	2.6336519857150715E-5	0.0
17	0.001498255351873463	3.8041639793662145E-5	0.0	2.6336519857150715E-5	0.0
18	0.0016709058709370066	3.8041639793662145E-5	0.0	2.9262799841278572E-5	0.0
19	0.001907934549651363	3.8041639793662145E-5	0.0	3.218907982540643E-5	0.0
20	0.009583566948018733	3.8041639793662145E-5	0.0	3.218907982540643E-5	0.0
21	0.009940573106082332	4.0967919777790006E-5	0.0	3.218907982540643E-5	0.0
22	0.010201012024669711	4.9746759730173575E-5	0.0	3.218907982540643E-5	0.0
23	0.010514123982971392	4.9746759730173575E-5	0.0	3.218907982540643E-5	0.0
24	0.010920876900765163	4.9746759730173575E-5	0.0	3.218907982540643E-5	0.0
25	0.011286661898781147	4.9746759730173575E-5	0.0	3.218907982540643E-5	0.0
26	0.01163488921689236	4.9746759730173575E-5	0.0	3.511535980953429E-5	0.0
27	0.011994821654940088	5.8525599682557145E-5	0.0	3.8041639793662145E-5	0.0
28	0.013024872209353094	5.8525599682557145E-5	0.0	3.8041639793662145E-5	0.0
29	0.0134667404869564	5.8525599682557145E-5	0.0	3.8041639793662145E-5	0.0
30	0.014455823121591615	5.8525599682557145E-5	0.0	4.0967919777790006E-5	0.0
31	0.039735955904472174	5.8525599682557145E-5	0.0	4.0967919777790006E-5	0.0
32	0.0404324105406946	6.437815965081286E-5	0.0	4.0967919777790006E-5	0.0
33	0.04188384541282202	6.730443963494072E-5	0.0	4.0967919777790006E-5	0.0
34	0.04246617512966347	6.730443963494072E-5	0.0	4.0967919777790006E-5	0.0
35	0.04314799836596526	6.730443963494072E-5	0.0	4.0967919777790006E-5	0.0
36	0.04387078952204484	7.023071961906858E-5	0.0	4.0967919777790006E-5	0.0
37	0.044567244158267266	7.023071961906858E-5	0.0	4.0967919777790006E-5	0.0
38	0.045339782074077024	7.023071961906858E-5	0.0	4.0967919777790006E-5	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCAACGC	33720	0.0	27.24502	12
CAACGCA	34255	0.0	26.646265	13
ATCAACG	35105	0.0	26.138817	11
AACGCAG	35210	0.0	25.873602	14
AAGCAGT	36405	0.0	25.850615	1
ACGCAGA	35900	0.0	25.345701	15
GTATCAA	37075	0.0	24.743988	9
CGCAGAG	37565	0.0	24.21081	16
GTGGTAT	37950	0.0	24.132933	6
GGTATCA	37995	0.0	24.115921	8
AGTGGTA	38735	0.0	23.655205	5
TGGTATC	39635	0.0	23.162424	7
AGAGTAC	38650	0.0	23.0444	19
TATCAAC	40110	0.0	22.964838	10
GAGTACT	22920	0.0	22.21451	20
CAGAGTA	40755	0.0	22.064205	18
GCAGAGT	42060	0.0	21.560665	17
AGCAGTG	44775	0.0	20.93978	2
GTACTTT	25115	0.0	20.339312	22
AGTACTT	26245	0.0	19.51892	21
>>END_MODULE