FastQCFastQC Report
Thu 26 May 2016
SRR522095_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR522095_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33733142
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1156800.34292684624515557No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA368350.10919528338036225No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC327850.028.13749912
CAACGCA332400.027.6597913
ATCAACG336250.027.51300611
AACGCAG347350.026.48214
ACGCAGA350700.026.2102415
AAGCAGT362050.025.964311
CGCAGAG356400.025.78097316
GGTATCA362900.025.5105678
GTGGTAT368500.025.0994116
GTATCAA369700.025.0710649
TGGTATC377400.024.4839277
AGTGGTA385400.024.1129325
AGAGTAC385900.023.4570419
TATCAAC401900.023.1828110
CAGAGTA403650.022.59432618
GCAGAGT406700.022.41397517
GAGTACT249000.022.18271820
GTACTTT250450.021.85832422
AGCAGTG433750.021.7120592
AGTACTT270550.020.36749621
Produced by FastQC (version 0.11.5)