##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR522095_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	33733142
Sequences flagged as poor quality	0
Sequence length	50
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.86616102941137	40.0	39.0	40.0	37.0	40.0
2	38.734758624026185	40.0	39.0	40.0	36.0	40.0
3	38.72350405426213	40.0	39.0	40.0	36.0	40.0
4	38.61205887669758	40.0	39.0	40.0	36.0	40.0
5	38.71748653001253	40.0	39.0	40.0	36.0	40.0
6	38.77649410778279	40.0	39.0	40.0	36.0	40.0
7	38.75166203017792	40.0	39.0	40.0	36.0	40.0
8	38.722266458309754	40.0	39.0	40.0	36.0	40.0
9	38.73077589392651	40.0	39.0	40.0	36.0	40.0
10	38.71614707577491	40.0	39.0	40.0	36.0	40.0
11	38.77724769901363	40.0	39.0	40.0	36.0	40.0
12	38.74443430143566	40.0	39.0	40.0	36.0	40.0
13	38.71872213978763	40.0	39.0	40.0	36.0	40.0
14	38.679564921642935	40.0	39.0	40.0	36.0	40.0
15	38.693973096250566	40.0	39.0	40.0	36.0	40.0
16	38.63884069856285	40.0	39.0	40.0	36.0	40.0
17	38.6021787416067	40.0	39.0	40.0	36.0	40.0
18	38.58857621386113	40.0	39.0	40.0	36.0	40.0
19	38.40222396123077	40.0	39.0	40.0	35.0	40.0
20	38.50713663138761	40.0	39.0	40.0	35.0	40.0
21	38.49605758633453	40.0	39.0	40.0	35.0	40.0
22	38.471963684853314	40.0	39.0	40.0	35.0	40.0
23	38.38935119058877	40.0	38.0	40.0	35.0	40.0
24	38.38733865941097	40.0	38.0	40.0	35.0	40.0
25	38.36459847114153	40.0	38.0	40.0	35.0	40.0
26	38.29605611597046	40.0	38.0	40.0	35.0	40.0
27	38.24816810719855	40.0	38.0	40.0	35.0	40.0
28	38.21327945081428	40.0	38.0	40.0	35.0	40.0
29	38.16435012190682	40.0	38.0	40.0	35.0	40.0
30	38.11864106818155	40.0	38.0	40.0	35.0	40.0
31	38.01388711433996	40.0	38.0	40.0	35.0	40.0
32	38.01070585123674	40.0	38.0	40.0	35.0	40.0
33	37.955712159869364	40.0	38.0	40.0	35.0	40.0
34	37.901248096011926	40.0	38.0	40.0	35.0	40.0
35	37.849636686674486	40.0	38.0	40.0	34.0	40.0
36	37.76126804316064	40.0	38.0	40.0	34.0	40.0
37	37.684850880478315	39.0	38.0	40.0	33.0	40.0
38	37.62893281627902	39.0	38.0	40.0	33.0	40.0
39	37.556328550717275	39.0	38.0	40.0	33.0	40.0
40	37.70260090210393	40.0	38.0	40.0	33.0	40.0
41	37.69478846648794	40.0	38.0	40.0	34.0	40.0
42	37.62546257920475	40.0	38.0	40.0	33.0	40.0
43	37.77914767026446	40.0	38.0	40.0	34.0	40.0
44	37.71636614816373	40.0	38.0	40.0	34.0	40.0
45	37.6712584318413	40.0	38.0	40.0	34.0	40.0
46	37.71298745311065	40.0	38.0	40.0	35.0	40.0
47	37.62465376038793	40.0	38.0	40.0	34.0	40.0
48	37.556381821770415	40.0	38.0	40.0	34.0	40.0
49	37.496712935901435	40.0	38.0	40.0	33.0	40.0
50	37.41917995661359	40.0	38.0	40.0	33.0	40.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	-0.09223324322276483
1	2	-0.16038693543378457
1	3	-0.1523264333519947
1	4	-0.014671562305601071
1	5	-0.12402297122067552
1	6	-0.18613538752034486
1	7	-0.18412925700788918
1	8	-0.19663895135952458
1	9	-0.20389538067592383
1	10	-0.19478173087656359
1	11	-0.211658353618148
1	12	-0.2065215696421916
1	13	-0.21102415313956158
1	14	-0.19575670115440147
1	15	-0.22196613901087403
1	16	-0.16793216115456744
1	17	-0.1472190145130554
1	18	-0.21309377748413993
1	19	-0.048824225458957926
1	20	-0.21270324219808145
1	21	-0.1915641184614003
1	22	-0.1769756988849167
1	23	-0.1463109716327864
1	24	-0.23410123388972437
1	25	-0.2046559116394988
1	26	-0.16146709392820213
1	27	-0.1868274047195655
1	28	-0.1775647767195565
1	29	-0.17013689905846263
1	30	-0.15857750480079602
1	31	-0.13130097288826192
1	32	-0.18764709399832213
1	33	-0.15624767938794548
1	34	-0.17547990296520766
1	35	-0.18307434341279105
1	36	-0.203939607914279
1	37	-0.20898101489783727
1	38	-0.2157038910724296
1	39	-0.20274618774728737
1	40	-0.13699832370078724
1	41	-0.1073189005313111
1	42	-0.09370517565303516
1	43	-0.1960322179357874
1	44	-0.16690444910627633
1	45	-0.18513103206811365
1	46	-0.07450451103229483
1	47	-0.09309689988203473
1	48	-0.11399354756697733
1	49	-0.10967517161507345
1	50	-0.1262064736109778
2	1	-0.14261130017907675
2	2	-0.1372371409312052
2	3	-0.11589235284364463
2	4	-0.023993275075589793
2	5	-0.12647673673014026
2	6	-0.156657088630908
2	7	-0.20605986397828246
2	8	-0.2168571769888885
2	9	-0.19972736831692828
2	10	-0.20403286524175002
2	11	-0.2249275600969014
2	12	-0.20522068838375418
2	13	-0.20739643867895552
2	14	-0.22053827148504013
2	15	-0.2527310169269441
2	16	-0.17073804139280924
2	17	-0.17072729756510086
2	18	-0.21483009951754184
2	19	-0.07264941176690343
2	20	-0.37433198657483757
2	21	-0.2527532635338332
2	22	-0.23341759121009176
2	23	-0.22242589472914176
2	24	-0.1984823805944771
2	25	-0.2781909142142851
2	26	-0.3253691158673533
2	27	-0.36743699848565825
2	28	-0.41518765251749556
2	29	-0.3605416561051271
2	30	-0.3588848473256405
2	31	-0.8609122281228565
2	32	-0.48032936486627165
2	33	-0.3155152341143861
2	34	-0.47793666106122146
2	35	-0.4097377454395854
2	36	-0.3876766489457495
2	37	-0.3804140169090857
2	38	-0.4635983343833132
2	39	-0.574233444963987
2	40	-0.3948758802733039
2	41	-0.3169935053252786
2	42	-0.313176964695117
2	43	-0.46254536879906993
2	44	-0.3237664306260797
2	45	-0.36737146890801853
2	46	-0.3292340997459675
2	47	-0.34345964412275976
2	48	-0.3616569787423458
2	49	-0.3871021690208494
2	50	-0.4908766186736955
3	1	-0.10093700613102641
3	2	-0.1308083975052341
3	3	-0.10472318723871155
3	4	-0.02919674799508698
3	5	-0.08889201027199078
3	6	-0.17642461045997493
3	7	-0.1800762801020781
3	8	-0.2420070602794624
3	9	-0.20893979288958064
3	10	-0.23273234901158446
3	11	-0.2581354889388763
3	12	-0.2500965103744548
3	13	-0.34655126813866843
3	14	-0.2137160906143123
3	15	-0.2817225613618817
3	16	-0.23739721113649637
3	17	-0.20335003544777663
3	18	-0.1772428893737228
3	19	-0.001492869689094789
3	20	-0.2742191658023998
3	21	-0.31430882875297783
3	22	-0.20852349552372118
3	23	-0.1968134115692024
3	24	-0.18110501506236432
3	25	-0.20963766587578903
3	26	-0.3091655808557192
3	27	-0.20786894344303164
3	28	-0.2433819952959766
3	29	-0.2942431192512771
3	30	-0.2398477653361084
3	31	-0.22160786780496977
3	32	-0.3409699878819481
3	33	-0.363298203256754
3	34	-0.29749695004281307
3	35	-0.35357640452625105
3	36	-0.37353322734854544
3	37	-0.33613711278432845
3	38	-0.41673332075225034
3	39	-0.37698834131879266
3	40	-0.4384407766216185
3	41	-0.39140860924956655
3	42	-0.2739396708134407
3	43	-0.4870767624344694
3	44	-0.3433381168690772
3	45	-0.4291033238858688
3	46	-0.4391957839185068
3	47	-0.4712371906956534
3	48	-0.3673416540772507
3	49	-0.4788957098371327
3	50	-0.4033765569060819
4	1	-0.06472034498042945
4	2	-0.11124390339950452
4	3	-0.10625668082335693
4	4	0.009734535575297798
4	5	-0.12280499821224566
4	6	-0.13441110384646038
4	7	-0.13243630804703344
4	8	-0.1705103187189536
4	9	-0.1395862017911469
4	10	-0.16750172971061517
4	11	-0.19795940005181478
4	12	-0.24339538548152717
4	13	-0.21673202616838694
4	14	-0.16763153719369228
4	15	-0.21692008426985865
4	16	-0.1654517635680719
4	17	-0.1934861028623729
4	18	-0.1846889329917616
4	19	-0.12312757462968449
4	20	-0.19885213309498084
4	21	-0.2256366109556467
4	22	-0.35381657683984713
4	23	-0.22871470847949382
4	24	-0.24951703824451954
4	25	-0.19607369809706654
4	26	-0.22887010206064673
4	27	-0.2972961137293879
4	28	-0.27506761870515817
4	29	-0.19362787757597033
4	30	-0.2078231398024002
4	31	-0.20568258597013767
4	32	-0.28681767143149983
4	33	-0.2793757582056742
4	34	-0.3448250803342603
4	35	-0.32515307370106683
4	36	-0.353979832941242
4	37	-0.2970133406622608
4	38	-0.322562549655224
4	39	-0.3041475052626623
4	40	-0.33636813161171375
4	41	-0.38103971552533267
4	42	-0.24499325097293934
4	43	-0.3967268283651464
4	44	-0.3891121618179625
4	45	-0.3819468422526242
4	46	-0.33221212172399106
4	47	-0.2848837072391319
4	48	-0.384598088914224
4	49	-0.5101604953099468
4	50	-0.4707698280284447
5	1	-0.11913329988729515
5	2	-0.2544754044019726
5	3	-0.14362201607600156
5	4	-0.06933474140736706
5	5	-0.13742294827484614
5	6	-0.18220817024872815
5	7	-0.14065912191446017
5	8	-0.12398185711680298
5	9	-0.12867115100237214
5	10	-0.17297222919080468
5	11	-0.21555209762133387
5	12	-0.1692940457165264
5	13	-0.17775963462664635
5	14	-0.16740130511448825
5	15	-0.22041192685543365
5	16	-0.13260573427879763
5	17	-0.19245865363876646
5	18	-0.1345006079350881
5	19	-0.03197413894739043
5	20	-0.14254041108896587
5	21	-0.13823184212543538
5	22	-0.17209706196060637
5	23	-0.2428346291594039
5	24	-0.1752687921257774
5	25	-0.16671412572350164
5	26	-0.2648851840594233
5	27	-0.18638036584668072
5	28	-0.2680371539310471
5	29	-0.2363724850059299
5	30	-0.20911814924678396
5	31	-0.17938960411368754
5	32	-0.1689358568075292
5	33	-0.32510442306202236
5	34	-0.2891463302342956
5	35	-0.29393111050055154
5	36	-0.22549752320365712
5	37	-0.20367962870430034
5	38	-0.23478143856534217
5	39	-0.33703977629561166
5	40	-0.32523702062798776
5	41	-0.3003233294301282
5	42	-0.2466718460845101
5	43	-0.33234241184691626
5	44	-0.2496080185322569
5	45	-0.2914665834573853
5	46	-0.18481003511307392
5	47	-0.254801938474742
5	48	-0.18267948117711796
5	49	-0.33458967445884014
5	50	-0.16829079569390615
6	1	-0.0530787097769192
6	2	-0.10111415698213477
6	3	-0.06660328546762173
6	4	0.01423023271313184
6	5	-0.07821430898945181
6	6	-0.0791304037998728
6	7	-0.07840758676078963
6	8	-0.08686384873469422
6	9	-0.11102021491412728
6	10	-0.13576425645140233
6	11	-0.1278306734347936
6	12	-0.14256371511808652
6	13	-0.12113543137446925
6	14	-0.13881381028232198
6	15	-0.14861400741863662
6	16	-0.11929848525760178
6	17	-0.13053305076216049
6	18	-0.15829866698611283
6	19	-0.00458186787327719
6	20	-0.10626513137754756
6	21	-0.14911641311437762
6	22	-0.15490479652338962
6	23	-0.1364545875404417
6	24	-0.10163817661983643
6	25	-0.20287143228610915
6	26	-0.14595304427736977
6	27	-0.20128573494309165
6	28	-0.16804297893803977
6	29	-0.18219416575764313
6	30	-0.20538727713242366
6	31	-0.16503123485055937
6	32	-0.2144918197243797
6	33	-0.17881207515035413
6	34	-0.20912911294932712
6	35	-0.19306564668053028
6	36	-0.28957960302261654
6	37	-0.22477584074917445
6	38	-0.3274668622518462
6	39	-0.23798210523438712
6	40	-0.30010778585303655
6	41	-0.1656010043675593
6	42	-0.21479269796301992
6	43	-0.29082582316647887
6	44	-0.2573231770523918
6	45	-0.1843995548056725
6	46	-0.2972540030702149
6	47	-0.19109422184429548
6	48	-0.2758893879312794
6	49	-0.3982236163162085
6	50	-0.22202502843080651
7	1	-0.0783491996866843
7	2	-0.07590089365886854
7	3	-0.0778822554077081
7	4	-0.03234825604580749
7	5	-0.10174213111545072
7	6	-0.14256234799427148
7	7	-0.16624842026180886
7	8	-0.14235748026603545
7	9	-0.13082002772663515
7	10	-0.185263627391258
7	11	-0.15417247857277516
7	12	-0.22414735771916838
7	13	-0.18714486182591372
7	14	-0.1330770793414331
7	15	-0.1632250194306124
7	16	-0.0686949036771054
7	17	-0.06996855281602166
7	18	-0.16972220964266427
7	19	0.011826376405132066
7	20	-0.16955988302643732
7	21	-0.17276960531444985
7	22	-0.07886619154349006
7	23	-0.07860828146381493
7	24	-0.14420143553819997
7	25	-0.14772833657766427
7	26	-0.20704379874302958
7	27	-0.25502080797848947
7	28	-0.235856996343486
7	29	-0.3293380404131412
7	30	-0.2749194454828796
7	31	-0.24462409248837957
7	32	-0.25040102767254524
7	33	-0.2971404977530554
7	34	-0.2789164801305759
7	35	-0.3409611647637689
7	36	-0.4094277165012059
7	37	-0.31716972733772764
7	38	-0.25738549778275654
7	39	-0.22427978075429422
7	40	-0.1829896239170452
7	41	-0.32394677718883713
7	42	-0.4623646922497855
7	43	-0.42118002639463725
7	44	-0.3986857566935953
7	45	-0.49725579606317183
7	46	-4.240136202415847
7	47	-7.973682273222455
7	48	-4.427262683937492
7	49	-4.377813170578946
7	50	-4.5601850773066275
8	1	-0.07363079657383054
8	2	-0.0522454102314569
8	3	-0.07022372290881407
8	4	0.03907769423909002
8	5	-0.09486975736705716
8	6	-0.08987079487469174
8	7	-0.10201708529248066
8	8	-0.06314893076030614
8	9	-0.08620580903397013
8	10	-0.05151396773921846
8	11	-0.11956714484709607
8	12	-0.15627641499555978
8	13	-0.14739630339661858
8	14	-0.11800718316877834
8	15	-0.14110391398143918
8	16	-0.11184900806881615
8	17	-0.1364295846737349
8	18	-0.10600901208195523
8	19	-0.0034137924634549677
8	20	-0.11193557249435315
8	21	-0.11580510044360892
8	22	-0.17100897225188305
8	23	-0.12210621721008863
8	24	-0.0787353103444346
8	25	-0.13405680253871566
8	26	-0.1569339942957484
8	27	-0.17603605278176104
8	28	-0.1725168282629781
8	29	-0.24059520112183463
8	30	-0.17849836712090195
8	31	-0.1436796895062784
8	32	-0.07940513737361954
8	33	-0.14318722534648032
8	34	-0.23470341857466792
8	35	-0.17393039686287892
8	36	-0.1620341499327438
8	37	-0.21707472914380332
8	38	-0.1651412535417407
8	39	-0.19767275727431155
8	40	-0.1303545396521386
8	41	-0.08784215754151603
8	42	-0.09752614046069397
8	43	-0.15927900357091573
8	44	-0.10130583110432667
8	45	-0.18185927759200382
8	46	-0.16000535037424157
8	47	-0.21697411246820053
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119	3	-0.06185821716119477
119	4	0.06063878562535763
119	5	-0.020042209115558762
119	6	-0.05327696255440628
119	7	-0.0715660515560117
119	8	-0.062653206404093
119	9	-0.07930126521372216
119	10	-0.045003513125323025
119	11	-0.0754767371232532
119	12	-0.0866850569270099
119	13	-0.07253995757123732
119	14	-0.06557898186864008
119	15	-0.09618797917061528
119	16	-0.02175154328695328
119	17	-0.028765548446664013
119	18	-0.09477346435456013
119	19	0.01623951447405858
119	20	-0.04284160863208797
119	21	-0.08101741008221808
119	22	-0.07817272671652375
119	23	-0.07723240757876937
119	24	-0.09010319552566415
119	25	-0.10739499994348023
119	26	-0.07193609660030376
119	27	-0.10681381140336299
119	28	-0.10861781479169252
119	29	-0.06042806813943713
119	30	-0.07237379412496381
119	31	-0.07202122310635417
119	32	-0.0931386597104904
119	33	-0.1573795701877998
119	34	-0.12901481499208245
119	35	-0.06451134728436614
119	36	-0.07475272674807343
119	37	-0.04712513813761632
119	38	-0.0756975112111462
119	39	-0.042771225782871625
119	40	0.039242188177169623
119	41	0.11904262442878633
119	42	0.06198479289371761
119	43	0.0892928608182757
119	44	0.07927741292047585
119	45	0.04809103526651626
119	46	0.08863182717307438
119	47	0.1679196781487704
119	48	0.1506785167469502
119	49	0.03927818337395195
119	50	0.11664915933407372
120	1	-0.029761420315487896
120	2	-0.02102130169771499
120	3	-0.051758109880267966
120	4	0.06590906342454161
120	5	-0.010555752061193857
120	6	-0.02839020955531879
120	7	-0.03580972884328304
120	8	-0.032647299120739603
120	9	-0.08300570178646893
120	10	-0.07124005616330464
120	11	-0.09986319579591196
120	12	-0.08429463012161165
120	13	-0.09666208340665605
120	14	-0.0628844474844712
120	15	-0.08783568181675605
120	16	-0.04400221317359865
120	17	-0.04570963959663743
120	18	-0.041684116629063794
120	19	0.10173529846656493
120	20	-0.04800963897052668
120	21	-0.036105135387451526
120	22	-0.08659615746956462
120	23	0.0026864821273449024
120	24	-0.020140182339368096
120	25	-0.03196679195181673
120	26	9.596066301682527E-4
120	27	-0.022594344201202432
120	28	-0.03996397430519494
120	29	0.0019656303544905995
120	30	-0.06719738906816275
120	31	0.011057165563229887
120	32	-0.017794912907213245
120	33	0.026413429701570124
120	34	0.007648359777626013
120	35	0.014993660656934082
120	36	0.030619333959137407
120	37	-0.010753241701856098
120	38	-0.006512914293146821
120	39	-0.006338200107897762
120	40	0.12152272777873918
120	41	0.12748186400270356
120	42	0.17311888824387012
120	43	0.05926294265174903
120	44	0.1351648544971198
120	45	0.08378764775705605
120	46	0.15589200229761246
120	47	0.20464907695038903
120	48	0.18983161461633102
120	49	0.1704712992932329
120	50	0.20388332281538624
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	29049.0
3	887.0
4	1351.0
5	1472.0
6	2565.0
7	3991.0
8	4611.0
9	5783.0
10	7505.0
11	9636.0
12	12464.0
13	17835.0
14	18100.0
15	18522.0
16	21022.0
17	22196.0
18	23988.0
19	28930.0
20	34832.0
21	34122.0
22	42550.0
23	53612.0
24	68088.0
25	81900.0
26	106104.0
27	134721.0
28	164754.0
29	201342.0
30	241665.0
31	289941.0
32	349907.0
33	446560.0
34	614643.0
35	908652.0
36	1434897.0
37	2496295.0
38	4392347.0
39	2.0886954E7
40	519349.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	14.486971717050956	30.676922339023672	25.457013727225743	29.379092216699632
2	23.47787973835942	30.230846866385818	25.960349555703417	20.33092383955134
3	22.679515511843846	28.315486776282704	25.882148601969057	23.122849109904394
4	23.235392165513	26.91189937781775	26.115795701648526	23.736912755020718
5	24.16180544974088	28.382244912841976	27.31032657061428	20.145623066802873
6	24.431182773895703	27.01227268269783	24.83164459141908	23.72489995198739
7	23.81357198377139	26.82197739781377	27.495771205779974	21.86867941263486
8	23.405765167087015	26.8562175441588	26.90837100202525	22.829646286728938
9	22.627874391303365	26.348408339786435	27.850758758256198	23.172958510654006
10	22.550170393258952	27.621962994137935	26.48694865127002	23.3409179613331
11	22.65471742893087	27.531473350451613	26.573910606963324	23.23989861365419
12	22.50625808885517	26.905409522777333	27.280773311896056	23.307559076471442
13	22.675180983734037	27.23979877119066	26.541734535134616	23.543285709940687
14	22.75863007365279	27.864712394712594	26.584022324395395	22.792635207239222
15	22.51279572702639	27.72509499265055	26.51042879618426	23.251680484138802
16	22.716249092540032	27.078202275743145	26.60100039448143	23.60454823723539
17	23.389626735629903	26.929039103443138	26.666730303391244	23.014603857535715
18	22.58620320633044	26.920599332253133	26.6473191260986	23.84587833531783
19	22.809004865304274	27.66343852582721	26.533342195043673	22.99421441382484
20	23.46437573324025	26.974765789261102	26.702572186414038	22.858286291084614
21	22.83206527278129	27.39109508387923	26.67057222241557	23.10626742092391
22	23.287881969986127	26.81874926104945	26.751409149787957	23.14195961917646
23	22.768854439945144	26.981717267843003	27.32276762123137	22.926660670980485
24	22.645084464822034	27.41160348441829	26.66090651686562	23.282405533894057
25	22.663631511111536	26.918865132693536	26.683867752372432	23.733635603822496
26	22.792672417219766	27.13967075973439	27.211002907392377	22.856653915653467
27	22.857344595322743	26.817931072359247	27.275702200317912	23.049022132000097
28	23.112038440696452	26.848281209645545	26.914919761057305	23.1247605886007
29	23.16540328018462	26.950110987960883	27.029644672718767	22.85484105913573
30	23.071310340629218	26.825790254671354	27.01933962938651	23.083559775312914
31	22.958533678726493	27.016413594506115	26.885215834367987	23.13983689239941
32	23.028229349736378	26.998094095853318	26.991456087018477	22.98222046739182
33	22.924182215537137	26.958111839741033	26.86371309147022	23.253992853251606
34	22.96008754992878	27.026232031650476	26.843625604732967	23.17005481368778
35	23.051907824569724	27.020396059530338	26.9112396948858	23.016456421014137
36	22.941959482814532	26.936179667022415	26.900839441662434	23.221021408500622
37	22.990240925934952	27.024047350749257	26.78832195664308	23.19738976667271
38	23.051871165177868	27.03018737779399	26.916440187700825	23.001501269327314
39	22.935329443468653	26.90858837790232	26.951634371681237	23.204447806947787
40	22.925446448766003	26.96938019640908	26.91791105824229	23.187262296582627
41	23.015062759045666	27.051508303019915	26.955893801491143	22.97753513644328
42	22.959714000446155	26.902669423454974	26.93466021894813	23.202956357150743
43	22.958234272135538	26.93562119770313	26.889767426081885	23.21637710407945
44	23.02296330408465	26.99341743508904	26.945868882735564	23.03775037809075
45	22.971866030571906	26.86879556878447	26.966875319571944	23.19246308107168
46	22.955064631392457	26.93793875937507	26.889912859362365	23.217083749870113
47	23.1260559503658	26.94911124551923	26.851796214251323	23.073036589863644
48	22.973021455815438	26.886094788786036	26.88697093154349	23.253912823855032
49	22.997278280583988	26.899067021560736	26.85358421123466	23.250070486620615
50	23.025783623124184	26.93678888205729	26.91787448689167	23.119553007926864
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	38463.0
1	21286.0
2	4109.0
3	2982.5
4	1856.0
5	1835.5
6	1815.0
7	2276.5
8	2738.0
9	3923.5
10	5109.0
11	8095.0
12	11081.0
13	16814.0
14	22547.0
15	34024.0
16	45501.0
17	67877.0
18	90253.0
19	125971.0
20	161689.0
21	214433.5
22	267178.0
23	399958.5
24	532739.0
25	539133.0
26	545527.0
27	635981.0
28	726435.0
29	811658.0
30	896881.0
31	1010235.0
32	1123589.0
33	1241387.0
34	1359185.0
35	1487980.0
36	1616775.0
37	1723293.0
38	1829811.0
39	1921700.5
40	2013590.0
41	2071582.5
42	2129575.0
43	2178533.0
44	2227491.0
45	2245914.5
46	2264338.0
47	2213933.0
48	2163528.0
49	2128717.0
50	2093906.0
51	2056908.5
52	2019911.0
53	1945655.0
54	1871399.0
55	1765753.0
56	1660107.0
57	1574542.5
58	1488978.0
59	1394480.0
60	1299982.0
61	1166567.0
62	1033152.0
63	903117.0
64	773082.0
65	663618.5
66	554155.0
67	459747.5
68	365340.0
69	295614.5
70	225889.0
71	180375.0
72	134861.0
73	102782.0
74	70703.0
75	52182.0
76	33661.0
77	24574.0
78	15487.0
79	10950.0
80	6413.0
81	4380.0
82	2347.0
83	1707.5
84	1068.0
85	762.5
86	457.0
87	332.0
88	207.0
89	137.5
90	68.0
91	51.5
92	35.0
93	32.0
94	29.0
95	30.5
96	32.0
97	26.5
98	21.0
99	35.0
100	49.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.121032307040951
2	0.0017994173208057524
3	9.041553259402874E-4
4	0.012207579122039684
5	4.80239878040415E-4
6	0.001001981967763335
7	3.6166213037611497E-4
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	1.7786662149645E-5
16	7.381464792102675E-4
17	0.0
18	0.0
19	0.0
20	4.950620964984525E-4
21	0.0
22	0.008519811169679955
23	0.0
24	6.610709432284725E-4
25	0.0
26	0.06472566356255816
27	0.03534802657872783
28	0.036821355093456756
29	0.05669498560199344
30	0.07548659416309338
31	0.11326250012524774
32	0.14330713693968974
33	0.16852269498050315
34	0.1264394523344431
35	0.132724072960651
36	0.12891476281693534
37	0.14283875483641575
38	0.15507004950798833
39	0.14989709526613323
40	0.12761930092370286
41	0.19152974247106896
42	0.22703488456545196
43	0.20634899648541483
44	0.2033756594627325
45	0.15227754355049405
46	0.17681424398592932
47	0.19623135016595844
48	0.18625599714369923
49	0.22017516186307223
50	0.2730193351096675
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	3.3733142E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.59826215585609
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.6880192707959	32.41759339028876
2	13.600725085221768	12.131374057208973
3	4.929025042386899	6.594778530394517
4	2.483649099833064	4.430657346300439
5	1.4876281724926652	3.317281561363249
6	0.9209057195911708	2.46424768218926
7	0.6545956709675129	2.043568053792836
8	0.4769874869713209	1.7018250391207972
9	0.3657605788472728	1.4681057563537512
>10	2.138532432491896	17.548007845498333
>50	0.14728164438404825	4.497887069503784
>100	0.10055205141205976	8.871282790070529
>500	0.005681207852638727	1.648223578026959
>1k	6.298763650829282E-4	0.3454820990104345
>5k	6.665096722566026E-6	0.020025849112561212
>10k+	1.9995290155589294E-5	0.4996593517647676
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	115680	0.34292684624515557	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	36835	0.10919528338036225	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.037555292062625E-4	2.9644436916075E-6	0.0	0.0	0.0
2	1.0968441658947749E-4	5.928887383215E-6	0.0	5.928887383215E-6	0.0
3	1.12648860281085E-4	1.185777476643E-5	0.0	8.8933310748225E-6	2.9644436916075E-6
4	1.156133039726925E-4	1.185777476643E-5	0.0	1.185777476643E-5	2.9644436916075E-6
5	1.2154219135590749E-4	1.4822218458037499E-5	0.0	1.4822218458037499E-5	2.9644436916075E-6
6	1.36364409813945E-4	1.7786662149645E-5	0.0	2.07511058412525E-5	2.9644436916075E-6
7	1.511866282719825E-4	1.7786662149645E-5	0.0	2.07511058412525E-5	2.9644436916075E-6
8	1.60079959346805E-4	1.7786662149645E-5	2.9644436916075E-6	2.6679993224467498E-5	2.9644436916075E-6
9	1.6600884673002E-4	2.07511058412525E-5	2.9644436916075E-6	2.9644436916074998E-5	2.9644436916075E-6
10	6.432842810788275E-4	2.6679993224467498E-5	2.9644436916075E-6	3.26088806076825E-5	2.9644436916075E-6
11	7.648264724347349E-4	2.9644436916074998E-5	2.9644436916075E-6	4.1502211682505E-5	2.9644436916075E-6
12	8.003997967340249E-4	3.26088806076825E-5	2.9644436916075E-6	4.44666553741125E-5	2.9644436916075E-6
13	8.389375647249224E-4	3.85377679908975E-5	2.9644436916075E-6	5.03955427573275E-5	2.9644436916075E-6
14	8.685820016409975E-4	4.1502211682505E-5	2.9644436916075E-6	5.3359986448934996E-5	2.9644436916075E-6
15	0.0011413108212688875	4.743109906572E-5	2.9644436916075E-6	5.9288873832149996E-5	2.9644436916075E-6
16	0.0011946708077178224	4.743109906572E-5	2.9644436916075E-6	6.225331752375749E-5	5.928887383215E-6
17	0.00129249744954087	4.743109906572E-5	2.9644436916075E-6	6.225331752375749E-5	5.928887383215E-6
18	0.0014318263030464224	4.743109906572E-5	2.9644436916075E-6	6.5217761215365E-5	5.928887383215E-6
19	0.0016571240236085923	5.03955427573275E-5	2.9644436916075E-6	7.411109229018749E-5	5.928887383215E-6
20	0.006767824947939922	5.03955427573275E-5	2.9644436916075E-6	7.7075535981795E-5	5.928887383215E-6
21	0.007129487078316037	5.03955427573275E-5	2.9644436916075E-6	8.300442336501E-5	5.928887383215E-6
22	0.007408144785327142	5.3359986448934996E-5	2.9644436916075E-6	8.8933310748225E-5	5.928887383215E-6
23	0.007710518041871107	5.63244301405425E-5	2.9644436916075E-6	8.8933310748225E-5	5.928887383215E-6
24	0.007974353530424175	5.9288873832149996E-5	2.9644436916075E-6	8.8933310748225E-5	5.928887383215E-6
25	0.008241153462668849	5.9288873832149996E-5	2.9644436916075E-6	8.8933310748225E-5	5.928887383215E-6
26	0.00856131338136246	5.9288873832149996E-5	2.9644436916075E-6	9.18977544398325E-5	5.928887383215E-6
27	0.008890366631130893	6.225331752375749E-5	2.9644436916075E-6	9.18977544398325E-5	5.928887383215E-6
28	0.009578117567583831	6.5217761215365E-5	2.9644436916075E-6	1.00791085514655E-4	5.928887383215E-6
29	0.009930886366885125	7.114664859858E-5	5.928887383215E-6	1.00791085514655E-4	5.928887383215E-6
30	0.01041112624492554	7.7075535981795E-5	5.928887383215E-6	1.00791085514655E-4	5.928887383215E-6
31	0.01715820008702421	8.00399796734025E-5	5.928887383215E-6	1.037555292062625E-4	5.928887383215E-6
32	0.017626582190298196	8.00399796734025E-5	5.928887383215E-6	1.037555292062625E-4	5.928887383215E-6
33	0.018429946430723827	8.00399796734025E-5	5.928887383215E-6	1.037555292062625E-4	5.928887383215E-6
34	0.01898429740105443	8.300442336501E-5	5.928887383215E-6	1.037555292062625E-4	5.928887383215E-6
35	0.01958607947045075	8.300442336501E-5	5.928887383215E-6	1.0671997289786999E-4	8.8933310748225E-6
36	0.020205648201996718	8.300442336501E-5	5.928887383215E-6	1.0968441658947749E-4	8.8933310748225E-6
37	0.020789643609243395	8.300442336501E-5	5.928887383215E-6	1.156133039726925E-4	8.8933310748225E-6
38	0.021515932313687234	8.8933310748225E-5	5.928887383215E-6	1.156133039726925E-4	8.8933310748225E-6
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCAACGC	32785	0.0	28.137499	12
CAACGCA	33240	0.0	27.65979	13
ATCAACG	33625	0.0	27.513006	11
AACGCAG	34735	0.0	26.482	14
ACGCAGA	35070	0.0	26.21024	15
AAGCAGT	36205	0.0	25.96431	1
CGCAGAG	35640	0.0	25.780973	16
GGTATCA	36290	0.0	25.510567	8
GTGGTAT	36850	0.0	25.099411	6
GTATCAA	36970	0.0	25.071064	9
TGGTATC	37740	0.0	24.483927	7
AGTGGTA	38540	0.0	24.112932	5
AGAGTAC	38590	0.0	23.45704	19
TATCAAC	40190	0.0	23.18281	10
CAGAGTA	40365	0.0	22.594326	18
GCAGAGT	40670	0.0	22.413975	17
GAGTACT	24900	0.0	22.182718	20
GTACTTT	25045	0.0	21.858324	22
AGCAGTG	43375	0.0	21.712059	2
AGTACTT	27055	0.0	20.367496	21
>>END_MODULE