FastQCFastQC Report
Thu 26 May 2016
SRR522094_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR522094_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26818908
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCG1977610.7373939311772127Illumina Paired End PCR Primer 2 (100% over 31bp)
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT404300.1507518501499017No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG282550.035.3598420
GATCGGA290700.034.53982521
ATGCCGA293250.034.07404314
TGCCGAG294000.033.99470515
CGAGATC297950.033.50685518
GAGATCG298550.033.48282619
ATCGGAA302450.033.19537422
CCGAGAT302850.033.0218417
TCGGAAG304650.032.9352523
GAGCGGT300200.032.1627729
AGAGCGG303350.032.12430228
AAGAGCG311100.032.08437727
AGCGGTT303300.031.83998130
GCCGAGA319050.031.43672216
GCGGTTC301800.031.40997331
CGGAAGA331750.030.25228524
GAAGAGC398600.025.23330526
GGAAGAG462100.022.11654525
AATGCCG507600.019.85251613
CGGTTCA523700.019.3985771
Produced by FastQC (version 0.11.5)