FastQCFastQC Report
Thu 26 May 2016
SRR522093_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR522093_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29129835
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCG1240410.42582115552662764Illumina Paired End PCR Primer 2 (100% over 31bp)
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT884730.30371953703136323No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA170100.034.1011421
AGATCGG173850.033.44867720
ATGCCGA177400.032.7201914
TGCCGAG183550.031.63595415
GAGCGGT181000.031.51358229
ATCGGAA184000.031.49099522
AGAGCGG187350.030.88342728
CGAGATC189150.030.70065318
AAGAGCG188000.030.68145427
TCGGAAG188750.030.6747223
GCGGTTC187850.030.37843931
GAGATCG192900.030.16165419
AGCGGTT191450.029.89855630
CCGAGAT197800.029.35798317
GCCGAGA210700.027.7065116
CGGAAGA215150.026.9312724
AAGCAGT256700.022.2078251
GAGTACT214900.021.13371520
TCAACGC261050.020.68569612
CAACGCA262350.020.52452913
Produced by FastQC (version 0.11.5)