##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR522093_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	29129835
Sequences flagged as poor quality	0
Sequence length	50
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	39.180119969783554	40.0	39.0	40.0	38.0	40.0
2	39.083874694106576	40.0	39.0	40.0	38.0	40.0
3	39.07598000469278	40.0	39.0	40.0	38.0	40.0
4	39.074603718146705	40.0	39.0	40.0	38.0	40.0
5	39.06987135354526	40.0	39.0	40.0	38.0	40.0
6	39.0960163351423	40.0	39.0	40.0	38.0	40.0
7	39.05759847249392	40.0	39.0	40.0	38.0	40.0
8	39.045879731210285	40.0	39.0	40.0	38.0	40.0
9	39.03648544524883	40.0	39.0	40.0	38.0	40.0
10	39.0156995396644	40.0	39.0	40.0	38.0	40.0
11	39.026283190412855	40.0	39.0	40.0	38.0	40.0
12	38.99082497377689	40.0	39.0	40.0	38.0	40.0
13	38.96096246339878	40.0	39.0	40.0	38.0	40.0
14	38.93866041465734	40.0	39.0	40.0	38.0	40.0
15	38.918298919303865	40.0	39.0	40.0	37.0	40.0
16	38.88819181433743	40.0	39.0	40.0	37.0	40.0
17	38.858318455974775	40.0	39.0	40.0	37.0	40.0
18	38.82206672300066	40.0	39.0	40.0	37.0	40.0
19	38.79308846067958	40.0	39.0	40.0	37.0	40.0
20	38.74270180383789	40.0	39.0	40.0	36.0	40.0
21	38.68475674510343	40.0	39.0	40.0	36.0	40.0
22	38.63237292624555	40.0	39.0	40.0	36.0	40.0
23	38.58535858510699	40.0	39.0	40.0	36.0	40.0
24	38.53813531727866	40.0	39.0	40.0	36.0	40.0
25	38.482246363565054	40.0	39.0	40.0	36.0	40.0
26	38.3279661213323	40.0	38.0	40.0	35.0	40.0
27	38.23595581643357	40.0	38.0	40.0	35.0	40.0
28	38.16990961328823	40.0	38.0	40.0	35.0	40.0
29	38.10970546177141	40.0	38.0	40.0	35.0	40.0
30	38.024341710140135	40.0	38.0	40.0	35.0	40.0
31	37.86019024824549	40.0	38.0	40.0	35.0	40.0
32	37.785259099476534	40.0	38.0	40.0	34.0	40.0
33	38.05186212005663	40.0	38.0	40.0	35.0	40.0
34	38.00916290806316	40.0	38.0	40.0	35.0	40.0
35	37.757243389809794	40.0	38.0	40.0	35.0	40.0
36	38.010696318739875	40.0	38.0	40.0	35.0	40.0
37	37.94396919858969	40.0	38.0	40.0	35.0	40.0
38	37.88093695003765	40.0	38.0	40.0	35.0	40.0
39	37.8394684006964	40.0	38.0	40.0	35.0	40.0
40	37.76840428378671	40.0	38.0	40.0	35.0	40.0
41	37.72090744763916	40.0	38.0	40.0	35.0	40.0
42	37.6441759797129	40.0	38.0	40.0	34.0	40.0
43	37.580191408567885	40.0	38.0	40.0	34.0	40.0
44	37.5117893046768	40.0	38.0	40.0	34.0	40.0
45	37.438861668801074	40.0	38.0	40.0	33.0	40.0
46	37.297118778736646	40.0	38.0	40.0	33.0	40.0
47	37.229673769178575	40.0	38.0	40.0	33.0	40.0
48	37.21210346711542	40.0	38.0	40.0	33.0	40.0
49	37.14887640111933	40.0	38.0	40.0	33.0	40.0
50	37.08347448586647	40.0	38.0	40.0	33.0	40.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	-0.0029725188753175757
1	2	-0.0022087479850867453
1	3	-0.009874782692953943
1	4	0.013421057809821946
1	5	0.01160044885322975
1	6	0.1062437641577958
1	7	0.2725481398827867
1	8	0.26900713143407273
1	9	0.1975617110364567
1	10	0.18263046099203706
1	11	0.26601625232367354
1	12	0.24608486818955555
1	13	0.21974019052959903
1	14	0.2462361078977935
1	15	0.23945552242598467
1	16	0.2074147568939395
1	17	0.19507685387826967
1	18	0.21648550776730247
1	19	0.20373181092730874
1	20	0.1853371298961548
1	21	0.16067243390313024
1	22	0.15871005748639533
1	23	0.11719115548638825
1	24	0.11316077274249636
1	25	0.08496794925569873
1	26	0.050035027500648255
1	27	0.02075245172243001
1	28	0.022714705103986432
1	29	-0.021699571914652438
1	30	-0.03272552262361472
1	31	-0.006381683829424389
1	32	-0.041092255573730085
1	33	0.053560736160171984
1	34	0.0412899808926781
1	35	0.17632130181068106
1	36	0.029873008594172745
1	37	0.02044794891742896
1	38	0.035533524812557005
1	39	0.002457964749183361
1	40	-0.036375449068877685
1	41	-0.07177980551206531
1	42	-0.08292885989481391
1	43	-0.07760286860136745
1	44	-0.06891508642479494
1	45	-0.07465396765722687
1	46	-0.09401882529834893
1	47	-0.09381241901368043
1	48	-0.028201408280395412
1	49	-0.0856270760942408
1	50	-0.05304844860829405
2	1	0.05715611417769395
2	2	0.06885319696834102
2	3	0.06970126135274768
2	4	0.08350127323204504
2	5	0.09079568875281296
2	6	0.19512226695840695
2	7	0.32151382070765777
2	8	0.337967132913235
2	9	0.2683310676430537
2	10	0.262587075450746
2	11	0.32278731529275007
2	12	0.30715379358253614
2	13	0.33999255070050083
2	14	0.3300763100518438
2	15	0.3053816840305288
2	16	0.27211449745693983
2	17	0.26370286918494656
2	18	0.25631859230506393
2	19	0.2566794810900461
2	20	0.24993600231715618
2	21	0.26497725480799517
2	22	0.26392740323684905
2	23	0.23292804893102925
2	24	0.23606402813860683
2	25	0.21249760849674004
2	26	0.21628462222741263
2	27	0.20176933188513146
2	28	0.1433812428196788
2	29	0.1687470076216826
2	30	0.15360683367863714
2	31	0.16738100485724772
2	32	0.0856996078590555
2	33	0.16882520822745306
2	34	0.13566379778407622
2	35	0.3152873724162717
2	36	0.1710001017949523
2	37	0.12889691335577425
2	38	0.11180730741099865
2	39	0.14125615838830186
2	40	0.11423916784866606
2	41	0.10183369747649351
2	42	0.10213670104526784
2	43	0.06389600787736072
2	44	0.053711623327579616
2	45	0.030415477927263623
2	46	0.07117059660258462
2	47	0.09286152593824681
2	48	0.1425385342435206
2	49	0.06861092697069893
2	50	0.09433220121861297
3	1	0.08406405061587208
3	2	0.11549070047364296
3	3	0.1266097122003913
3	4	0.122260474252883
3	5	0.12963086841811844
3	6	0.20165850732816892
3	7	0.34190894164486707
3	8	0.3530989100375237
3	9	0.2656316977775717
3	10	0.27025288424192695
3	11	0.3169751348824832
3	12	0.31651095427348963
3	13	0.3474268834250367
3	14	0.35603640322759844
3	15	0.328456792950341
3	16	0.32865207492330484
3	17	0.3421609201329474
3	18	0.30952720315273297
3	19	0.3119890028470209
3	20	0.3484892648019269
3	21	0.3364072868138308
3	22	0.3377085260695907
3	23	0.32588066562391305
3	24	0.3330391309865064
3	25	0.31366780451555343
3	26	0.31250041627763636
3	27	0.26836168423625395
3	28	0.2969251617535491
3	29	0.26505916538404506
3	30	0.2896010468450214
3	31	0.33666123569298634
3	32	0.27283038395112413
3	33	0.34137480440707435
3	34	0.3055960268925091
3	35	0.44863351803244456
3	36	0.2593965896999748
3	37	0.1363426995266792
3	38	0.1430496209093164
3	39	0.15488957821695948
3	40	0.29406643356944073
3	41	0.18727997142961073
3	42	0.17844332392463258
3	43	0.10865359855221612
3	44	0.10240444198558407
3	45	0.13755867395105525
3	46	-0.042148877934621964
3	47	0.005792648489752139
3	48	0.07481427942799002
3	49	0.12314789688264227
3	50	0.15744381492361725
4	1	0.08580494016590023
4	2	0.1171431472155291
4	3	0.14225499945085573
4	4	0.14228118816513557
4	5	0.14069927180631225
4	6	0.19500388903272636
4	7	0.3095822978726872
4	8	0.2590843051352323
4	9	0.23302499624136175
4	10	0.28797554767555766
4	11	0.3376364588603309
4	12	0.33798317905150554
4	13	0.35124187611432944
4	14	0.31958362086324854
4	15	0.3220361799592979
4	16	0.36608278476685285
4	17	0.3674403395635295
4	18	0.3950961054040505
4	19	0.3438619005238266
4	20	0.39983536766758476
4	21	0.3932765530362303
4	22	0.34036404841340584
4	23	0.3763565818086434
4	24	0.38435837280648855
4	25	0.34987472594021796
4	26	0.35685690242887347
4	27	0.35217665997608094
4	28	0.3325842598625144
4	29	0.3821673446790257
4	30	0.3643394570931875
4	31	0.4183637973724501
4	32	0.41072986391915123
4	33	0.4134466302191484
4	34	0.38878906522442946
4	35	0.5639782097783197
4	36	0.37237655666174874
4	37	0.39243552885567823
4	38	0.4033399922927785
4	39	0.4245997802261101
4	40	0.41648476513717725
4	41	0.4292300538120273
4	42	0.45643608230536614
4	43	0.4150304209380238
4	44	0.4471897296314111
4	45	0.4053286016666675
4	46	0.2990194702053799
4	47	0.25400221482665586
4	48	0.24085733364329087
4	49	0.20380195583299354
4	50	0.12090983051007242
5	1	0.08273224461062512
5	2	0.11534971308083897
5	3	0.12627418004547053
5	4	0.15108320850281132
5	5	0.1434413902201115
5	6	0.24010847765966759
5	7	0.39904060766640725
5	8	0.4000124443103914
5	9	0.28703691154616706
5	10	0.3208031188915612
5	11	0.3642866049727118
5	12	0.35174932461632125
5	13	0.40035121856289635
5	14	0.3858594421245698
5	15	0.3742829053983314
5	16	0.37493442753858375
5	17	0.39029364243265974
5	18	0.389362411875652
5	19	0.3857742894828533
5	20	0.38243517659297765
5	21	0.3843234927603021
5	22	0.4198188609933524
5	23	0.3968847128618407
5	24	0.40579447925068735
5	25	0.3922178380442105
5	26	0.41474347812398804
5	27	0.4101209487620352
5	28	0.3839455513358203
5	29	0.4086869226665044
5	30	0.39320078204934106
5	31	0.4532929271183761
5	32	0.4336297037139758
5	33	0.4144349005534451
5	34	0.4363019974624578
5	35	0.6573703301750129
5	36	0.49352126383533346
5	37	0.4454330800235766
5	38	0.47668457108260753
5	39	0.4883937912178453
5	40	0.47054372205563766
5	41	0.45191266781872486
5	42	0.4768801625978014
5	43	0.4472694112188407
5	44	0.4823911900497251
5	45	0.4482129544525293
5	46	0.47061729132526153
5	47	0.4519555486121618
5	48	0.5232757678839377
5	49	0.5361548347577099
5	50	0.5222496362297235
6	1	0.11810068221156911
6	2	0.17803468705036352
6	3	0.16608650982158935
6	4	0.1928388429849548
6	5	0.1797566577019296
6	6	0.27430182345260334
6	7	0.42960878995110363
6	8	0.426809934792864
6	9	0.34244490967755326
6	10	0.350953081694513
6	11	0.4038627573170075
6	12	0.40117434465872037
6	13	0.42646791608750334
6	14	0.41725743557682193
6	15	0.41133204379002564
6	16	0.4172900668047106
6	17	0.4155324335956294
6	18	0.4327947318264833
6	19	0.4394749035923553
6	20	0.4467258955868445
6	21	0.4445276011886037
6	22	0.47555340380133515
6	23	0.45114444402462084
6	24	0.462461096888255
6	25	0.4652474303775094
6	26	0.4950259173067266
6	27	0.4778921751182281
6	28	0.4519369044231496
6	29	0.4497126234777795
6	30	0.44520767931917504
6	31	0.5594754018649084
6	32	0.520258723352768
6	33	0.5316808832138022
6	34	0.5737378584826658
6	35	0.6980364002676964
6	36	0.544743090207028
6	37	0.5833157177500965
6	38	0.5650903826421754
6	39	0.5556355244393103
6	40	0.6044613324802555
6	41	0.5935251215277475
6	42	0.6060764318866418
6	43	0.5685678331805946
6	44	0.5960986774271149
6	45	0.6209056445899606
6	46	0.6270782339501508
6	47	0.615584648406525
6	48	0.6979490784889961
6	49	0.6818994690996547
6	50	0.6437129232918366
7	1	0.0947245824346723
7	2	0.13576085434020513
7	3	0.14861896188885737
7	4	0.13840615373919718
7	5	0.15042766094637727
7	6	0.26935459205998313
7	7	0.39470124621777103
7	8	0.4166324321936514
7	9	0.341287456793232
7	10	0.34264122939323727
7	11	0.39044248283465066
7	12	0.3963481454146205
7	13	0.4131242211353765
7	14	0.43005663633793034
7	15	0.39331252616703694
7	16	0.3971400547359565
7	17	0.43049616355214226
7	18	0.44172175430053784
7	19	0.4197347555200963
7	20	0.4438923226441034
7	21	0.45591373509007127
7	22	0.43883852376969656
7	23	0.45801891259693406
7	24	0.4769649237587501
7	25	0.4184181729904992
7	26	0.44191899522349587
7	27	0.4327487529828673
7	28	0.4192070835233608
7	29	0.4470474911067228
7	30	0.41394036111563537
7	31	0.5360571764942819
7	32	0.4860363875888183
7	33	0.4942175356141689
7	34	0.46216767668796876
7	35	0.6571645165601865
7	36	0.49738119946785986
7	37	0.5134231075827316
7	38	0.5353497631862254
7	39	0.541943956780301
7	40	0.5577659757413826
7	41	0.5716650567605512
7	42	0.5635804234156296
7	43	0.5679531050350093
7	44	0.5520257645190938
7	45	0.5925819963431849
7	46	0.6017099875184044
7	47	0.6014731804499007
7	48	0.6584683685318069
7	49	0.6492054255308304
7	50	0.6437031637615434
8	1	0.10307822244584486
8	2	0.12271697801951831
8	3	0.14380335687792467
8	4	0.15922290998413047
8	5	0.15625550718511505
8	6	0.2424826378913565
8	7	0.42202165030771965
8	8	0.40069310018185433
8	9	0.3421296423667073
8	10	0.3369423962949156
8	11	0.41056500410523
8	12	0.41282308734201223
8	13	0.4320485530449929
8	14	0.43090207806437064
8	15	0.43580891238219976
8	16	0.42167038532403467
8	17	0.4269304921238657
8	18	0.43152862628195265
8	19	0.41026336947523134
8	20	0.44068816594003835
8	21	0.4420680072581362
8	22	0.4121311756269961
8	23	0.44233544063867924
8	24	0.46010248600372705
8	25	0.4695446384296389
8	26	0.4373908036145622
8	27	0.4820708941418701
8	28	0.4579596022734833
8	29	0.4812967843580438
8	30	0.49452368252790535
8	31	0.5834655375866618
8	32	0.5504945650968409
8	33	0.5312218953168895
8	34	0.55153388490465
8	35	0.679713811855116
8	36	0.5448467544103366
8	37	0.5473196123273141
8	38	0.5628639560621949
8	39	0.5625603438129048
8	40	0.5934908035671853
8	41	0.5765893137377773
8	42	0.5689786345751102
8	43	0.582543660828982
8	44	0.5646511230024629
8	45	0.5916338223092978
8	46	0.6045425408736733
8	47	0.6062644733061191
8	48	0.6493010649398201
8	49	0.6563130829399455
8	50	0.7115707786635568
9	1	0.11210877576444744
9	2	0.16077244065554197
9	3	0.1570628914543164
9	4	0.15374735470388856
9	5	0.16511668540477586
9	6	0.27017188744568443
9	7	0.4152233545207551
9	8	0.42934958011468183
9	9	0.33510894540138736
9	10	0.348858665472477
9	11	0.40835862638389386
9	12	0.3891095754655254
9	13	0.4288779390189674
9	14	0.436807424034761
9	15	0.41726319941407297
9	16	0.43734443737274376
9	17	0.4398179562465998
9	18	0.4466761136689854
9	19	0.4346734681075972
9	20	0.4585980339942992
9	21	0.4760800454996854
9	22	0.47251204934590874
9	23	0.4722316736120362
9	24	0.48096296904763136
9	25	0.46912834516182755
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119	24	0.04157838285347282
119	25	0.027662477167126553
119	26	-0.11245621557571894
119	27	-0.19929896488878995
119	28	-0.1536981610664654
119	29	-0.27256577554891237
119	30	-0.3472594091723451
119	31	-0.24176331199925016
119	32	-0.2698683056244491
119	33	-0.2615207002835831
119	34	-0.2645077942948575
119	35	-0.11037139137735608
119	36	-0.23997725449299168
119	37	-0.2680403908810973
119	38	-0.25573449187138664
119	39	-0.3118554255026993
119	40	-0.3355345227878672
119	41	-0.390218592977817
119	42	-0.38902081398011035
119	43	-0.38765072847098025
119	44	-0.38657463849265383
119	45	-0.4283486116677224
119	46	-0.5349470919643622
119	47	-0.5451127249533201
119	48	-0.4876531350641926
119	49	-0.5711274512596631
119	50	-0.5530954067422087
120	1	-0.08266616085864342
120	2	-0.08836632117630927
120	3	-0.07144203824982043
120	4	-0.07198205034870853
120	5	-0.056131844593544145
120	6	0.0075316618048262285
120	7	0.16658765132384445
120	8	0.16389380532118025
120	9	0.08718000963702366
120	10	0.08367007129113802
120	11	0.12934576507013418
120	12	0.13059580292802764
120	13	0.13662531198748695
120	14	0.11564770754146281
120	15	0.07419595292525116
120	16	0.06471401366690088
120	17	0.08071041410583035
120	18	0.057280790476575305
120	19	0.027510361376954506
120	20	0.014572345698894651
120	21	0.010140928653306958
120	22	0.007058812733660602
120	23	-0.028638704662832026
120	24	-0.09299175072477794
120	25	-0.07857109938942841
120	26	-0.1992645404730098
120	27	-0.26401172107374293
120	28	-0.2832026486611454
120	29	-0.303947427336297
120	30	-0.3625310457314228
120	31	-0.40031072214867436
120	32	-0.5159102650829226
120	33	-0.3767735788499422
120	34	-0.3760552078251109
120	35	-0.3121686641040924
120	36	-0.36069589163358984
120	37	-0.36329746208259905
120	38	-0.43611107742945876
120	39	-0.41954030584528823
120	40	-0.44142116965425515
120	41	-0.46498590255866645
120	42	-0.4895808637081771
120	43	-0.5229506551231324
120	44	-0.5451334724343013
120	45	-0.5683353727468514
120	46	-0.6173396300008207
120	47	-0.6392682218097008
120	48	-0.5870104597578916
120	49	-0.6507911674558642
120	50	-0.6966850570040464
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	8382.0
3	693.0
4	1129.0
5	5588.0
6	2434.0
7	5122.0
8	3926.0
9	3680.0
10	5347.0
11	9542.0
12	8852.0
13	11365.0
14	16614.0
15	21060.0
16	20700.0
17	22810.0
18	30212.0
19	37268.0
20	43988.0
21	33985.0
22	43639.0
23	54025.0
24	56561.0
25	62595.0
26	81670.0
27	102072.0
28	115311.0
29	137298.0
30	169887.0
31	209341.0
32	251911.0
33	324202.0
34	450203.0
35	664061.0
36	1079496.0
37	2015017.0
38	4171028.0
39	1.8397407E7
40	451414.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	17.50209719078469	29.395163238019972	25.096284572900373	28.00645499829497
2	23.53181015630171	29.63178002909459	26.288490802688212	20.54791901191549
3	23.336838336287354	28.029501757479196	25.858487136364527	22.77517276986892
4	23.002989889918705	26.920516370930354	26.7414353702999	23.33505836885104
5	23.8336004365562	28.05449276500652	27.40271032301891	20.70919647541838
6	23.992675683978675	27.01767559901419	25.333346561473657	23.656302155533478
7	23.391220196767502	27.401260083742397	27.201746252378577	22.005773467111524
8	23.38109444770609	27.02087832014422	26.953074981477755	22.644952250671942
9	22.59171739215138	26.522917139764097	27.48888553608354	23.39647993200099
10	22.504518134071134	27.822375238308076	26.608643680954597	23.064462946666193
11	23.063683676890033	27.60942861502648	26.494396552537975	22.832491155545508
12	22.90822450590606	27.070984095859107	27.098505707292887	22.92228569094195
13	22.56471569885073	27.6384008106054	26.554742006244936	23.24214148429893
14	22.712044197984643	28.120790934792456	26.670233456523185	22.496931410699716
15	22.43858464163325	27.49497291855656	27.11064041970873	22.95580202010146
16	22.964916198509027	27.16402118763422	26.733721820211333	23.137340793645425
17	23.13861716003541	26.961172282644235	26.799513282516017	23.10069727480434
18	22.445149449009925	26.936475953262352	26.79966089749564	23.81871370023208
19	23.209421407539967	27.33200296740489	26.680749595861013	22.777826029194127
20	23.133409566913848	27.297524147976453	26.89329163944652	22.675774645663182
21	23.061845906421585	27.294434009858833	26.763318331880974	22.88040175183861
22	23.055466267719115	27.226563710953766	26.711537967047622	23.006432054279497
23	22.6506980535163	26.915540457731296	27.71619912457428	22.717562364178114
24	22.609159513728848	27.082200757191377	26.86681275325478	23.441826975824995
25	23.184486289535368	26.73619047573274	26.728232747951708	23.351090486780183
26	23.296941969645605	26.848452140331435	27.173880161465153	22.680725728557807
27	22.821115702561652	27.10304992183025	27.151838615272062	22.923995760336034
28	22.87765878671429	27.219408411455564	26.95507872121608	22.947854080614064
29	23.322257798902907	26.822787029981566	27.101838017578483	22.75311715353705
30	22.82133117703154	27.123720631704657	27.078296244076988	22.976651947186816
31	23.271881171362352	26.77099554480526	26.97257277605668	22.98455050777571
32	22.873623227290242	26.791209660022535	27.039750249095263	23.295416863591964
33	23.22826728793158	26.70065791880078	26.9827212470615	23.088353546206136
34	23.309538994079908	26.789586053102255	26.936395611520496	22.96447934129734
35	22.921749762087856	26.830920219125527	27.38534476310334	22.86198525568327
36	22.818520639936118	26.691837725526767	27.441444388361134	23.04819724617598
37	22.870403132211717	26.77469011198457	26.87573961813388	23.479167137669833
38	22.884342541331293	27.284916240276928	26.987067927440915	22.84367329095086
39	23.253342897066133	26.68695821758335	26.984847924691515	23.074850960659003
40	22.874592046017376	26.738724336073272	26.89636759477973	23.490316023129623
41	22.91992524131091	27.26144965773627	26.990313339956312	22.828311760996502
42	23.341998275487295	26.66926871493841	26.935669985967632	23.053063023606665
43	23.330535485992268	26.72785855421496	26.880278818142557	23.06132714165021
44	22.928650754162533	27.218505089600608	26.978312501202645	22.87453165503421
45	22.83696530027889	27.08718854953917	26.96094613213504	23.114900018046907
46	22.90666639625554	26.73726574132208	27.296050857458216	23.060017004964166
47	23.416124812737213	26.77268020366988	26.92250761655331	22.888687367039598
48	22.885200585579067	26.622426275849815	26.878508842635767	23.613864295935354
49	22.92642380673489	26.691620197029454	26.81378699509652	23.56816900113914
50	23.405030209748134	26.749889107633386	26.8830458840026	22.96203479861589
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	15852.0
1	8643.0
2	1434.0
3	1186.0
4	938.0
5	968.5
6	999.0
7	1298.0
8	1597.0
9	2509.0
10	3421.0
11	5674.0
12	7927.0
13	12837.0
14	17747.0
15	26820.0
16	35893.0
17	54519.0
18	73145.0
19	103124.0
20	133103.0
21	182350.0
22	231597.0
23	330142.5
24	428688.0
25	442033.0
26	455378.0
27	532519.5
28	609661.0
29	685524.5
30	761388.0
31	865988.5
32	970589.0
33	1077115.5
34	1183642.0
35	1307323.0
36	1431004.0
37	1521397.5
38	1611791.0
39	1690798.5
40	1769806.0
41	1825824.0
42	1881842.0
43	1924131.0
44	1966420.0
45	1971328.0
46	1976236.0
47	1943046.0
48	1909856.0
49	1870659.0
50	1831462.0
51	1781384.0
52	1731306.0
53	1664655.0
54	1598004.0
55	1508566.5
56	1419129.0
57	1347625.0
58	1276121.0
59	1246022.0
60	1215923.0
61	1023424.0
62	830925.0
63	716725.0
64	602525.0
65	514566.5
66	426608.0
67	354820.0
68	283032.0
69	230003.5
70	176975.0
71	144973.0
72	112971.0
73	88952.5
74	64934.0
75	49718.0
76	34502.0
77	26987.0
78	19472.0
79	15370.5
80	11269.0
81	8732.5
82	6196.0
83	4884.5
84	3573.0
85	2845.0
86	2117.0
87	1719.5
88	1322.0
89	1012.0
90	702.0
91	535.5
92	369.0
93	282.0
94	195.0
95	133.0
96	71.0
97	57.5
98	44.0
99	89.0
100	134.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.07500557418193408
2	6.52252235551626E-5
3	1.5448079263064826E-4
4	0.0
5	7.895684956677578E-5
6	5.492650404645272E-5
7	8.582266257258238E-5
8	3.432906502903295E-6
9	0.0
10	0.0
11	0.0
12	0.0
13	6.86581300580659E-6
14	0.0
15	3.7761971531936244E-4
16	0.001991085771683911
17	0.0
18	0.0
19	1.7164532514516475E-5
20	2.2313892268871417E-4
21	7.243432721125953E-4
22	0.004098890364466534
23	0.0023790042065119834
24	2.711996137293603E-4
25	0.0031479752631623213
26	0.02494349865009534
27	0.042681326550596664
28	0.04779635723992258
29	0.04809158719917226
30	0.04571601589916318
31	0.03936513886879208
32	0.04622065315508996
33	0.0457400462446835
34	0.05701371120021792
35	0.08789613810033596
36	0.09030260555887117
37	0.08681477255192142
38	0.09188517545670959
39	0.08732970852735693
40	0.08105435544004969
41	0.0931381863302693
42	0.09310385726524026
43	0.08281886938254199
44	0.09390715738691963
45	0.11477579601806877
46	0.13158330625628328
47	0.111075122807939
48	0.12792726083069128
49	0.12641334906291093
50	0.13238317347145978
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2.9129835E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.8190209406055
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.64375155701653	30.67715298183429
2	14.293398856298534	12.240586898805471
3	4.940186890928938	6.34601897799573
4	2.4704821432702553	4.23134506504324
5	1.4903419232092887	3.1907491009280404
6	0.9590019631335631	2.463811508489869
7	0.6731524844615805	2.0176611229866723
8	0.4790840069773676	1.6411126501658473
9	0.36331722225617546	1.400119897308282
>10	2.3372597780011364	19.684104980406474
>50	0.23302684950340072	6.833118433340105
>100	0.1167321876177474	8.32104553393114
>500	1.7542362695091235E-4	0.043029281107455436
>1k	3.238621997471249E-5	0.02808875088690293
>5k	2.4140347988293057E-5	0.06160994307558937
>10k+	3.218713058109258E-5	0.8204448736949081
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCG	124041	0.42582115552662764	Illumina Paired End PCR Primer 2 (100% over 31bp)
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	88473	0.30371953703136323	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.0298719508709884E-5	3.432906502903295E-6	0.0	0.0	0.0
2	1.373162601161318E-5	3.432906502903295E-6	0.0	0.0	0.0
3	1.373162601161318E-5	3.432906502903295E-6	0.0	0.0	0.0
4	2.4030345520323065E-5	6.86581300580659E-6	0.0	0.0	0.0
5	3.432906502903295E-5	6.86581300580659E-6	0.0	0.0	0.0
6	4.1194878034839537E-5	6.86581300580659E-6	0.0	3.432906502903295E-6	0.0
7	4.4627784537742833E-5	1.0298719508709884E-5	0.0	3.432906502903295E-6	0.0
8	5.8359410549356015E-5	1.0298719508709884E-5	0.0	3.432906502903295E-6	0.0
9	7.895684956677578E-5	1.0298719508709884E-5	0.0	3.432906502903295E-6	0.0
10	5.629966664761404E-4	1.0298719508709884E-5	0.0	6.86581300580659E-6	0.0
11	6.316547965342063E-4	1.0298719508709884E-5	0.0	6.86581300580659E-6	0.0
12	7.724039631532414E-4	1.0298719508709884E-5	0.0	1.0298719508709884E-5	3.432906502903295E-6
13	8.650924387316303E-4	1.0298719508709884E-5	0.0	1.0298719508709884E-5	3.432906502903295E-6
14	9.371834752925995E-4	1.0298719508709884E-5	0.0	1.373162601161318E-5	3.432906502903295E-6
15	0.005266078575453654	1.0298719508709884E-5	0.0	1.7164532514516475E-5	3.432906502903295E-6
16	0.005492650404645272	1.0298719508709884E-5	0.0	2.0597439017419768E-5	3.432906502903295E-6
17	0.006319980871844966	1.0298719508709884E-5	0.0	2.4030345520323065E-5	3.432906502903295E-6
18	0.007219402375605629	1.0298719508709884E-5	0.0	2.746325202322636E-5	3.432906502903295E-6
19	0.009299743716365026	1.373162601161318E-5	0.0	2.746325202322636E-5	3.432906502903295E-6
20	0.45932289008846083	1.373162601161318E-5	0.0	2.746325202322636E-5	3.432906502903295E-6
21	0.46574929106189583	1.373162601161318E-5	0.0	3.432906502903295E-5	3.432906502903295E-6
22	0.4674520126873358	1.373162601161318E-5	0.0	3.432906502903295E-5	3.432906502903295E-6
23	0.4682106850244775	1.373162601161318E-5	0.0	3.432906502903295E-5	3.432906502903295E-6
24	0.4686329325243346	1.373162601161318E-5	0.0	3.432906502903295E-5	6.86581300580659E-6
25	0.46899338770713944	1.373162601161318E-5	0.0	3.432906502903295E-5	6.86581300580659E-6
26	0.4693778732354646	1.373162601161318E-5	0.0	3.7761971531936246E-5	6.86581300580659E-6
27	0.46984131561335657	1.373162601161318E-5	0.0	4.1194878034839537E-5	6.86581300580659E-6
28	0.4831644257511242	1.373162601161318E-5	0.0	4.1194878034839537E-5	6.86581300580659E-6
29	0.4838681715842194	1.373162601161318E-5	0.0	4.1194878034839537E-5	6.86581300580659E-6
30	0.4849975978236746	1.373162601161318E-5	0.0	4.4627784537742833E-5	6.86581300580659E-6
31	0.5045136712926799	1.373162601161318E-5	0.0	4.4627784537742833E-5	6.86581300580659E-6
32	0.505217417125775	1.373162601161318E-5	0.0	4.4627784537742833E-5	6.86581300580659E-6
33	0.5056602620646495	1.373162601161318E-5	0.0	4.806069104064613E-5	6.86581300580659E-6
34	0.506068777938495	1.373162601161318E-5	0.0	5.149359754354942E-5	6.86581300580659E-6
35	0.5064017698692767	1.373162601161318E-5	0.0	5.492650404645272E-5	6.86581300580659E-6
36	0.5067828224910988	1.373162601161318E-5	0.0	6.522522355516261E-5	6.86581300580659E-6
37	0.5073011913730373	1.373162601161318E-5	0.0	6.522522355516261E-5	6.86581300580659E-6
38	0.5077989628159583	1.373162601161318E-5	0.0	6.522522355516261E-5	6.86581300580659E-6
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATCGGA	17010	0.0	34.10114	21
AGATCGG	17385	0.0	33.448677	20
ATGCCGA	17740	0.0	32.72019	14
TGCCGAG	18355	0.0	31.635954	15
GAGCGGT	18100	0.0	31.513582	29
ATCGGAA	18400	0.0	31.490995	22
AGAGCGG	18735	0.0	30.883427	28
CGAGATC	18915	0.0	30.700653	18
AAGAGCG	18800	0.0	30.681454	27
TCGGAAG	18875	0.0	30.67472	23
GCGGTTC	18785	0.0	30.378439	31
GAGATCG	19290	0.0	30.161654	19
AGCGGTT	19145	0.0	29.898556	30
CCGAGAT	19780	0.0	29.357983	17
GCCGAGA	21070	0.0	27.70651	16
CGGAAGA	21515	0.0	26.93127	24
AAGCAGT	25670	0.0	22.207825	1
GAGTACT	21490	0.0	21.133715	20
TCAACGC	26105	0.0	20.685696	12
CAACGCA	26235	0.0	20.524529	13
>>END_MODULE