FastQCFastQC Report
Thu 26 May 2016
SRR522092_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR522092_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33218769
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCG2581960.7772593861018752Illumina Paired End PCR Primer 2 (100% over 31bp)
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1482550.4462988980717497No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA333700.035.90064621
AGATCGG334200.035.8805420
ATGCCGA340900.035.20868714
ATCGGAA345900.034.592422
TGCCGAG348200.034.54015415
CGAGATC352500.034.0968918
GAGATCG353150.034.04730219
GAGCGGT350050.033.78867729
TCGGAAG359450.033.32882323
CCGAGAT361150.033.3042817
AGAGCGG358700.033.2182328
AAGAGCG359900.033.0567327
GCGGTTC359650.032.90725331
AGCGGTT360900.032.7805930
GCCGAGA377200.031.887716
CGGAAGA395600.030.24421324
AAGCAGT360200.026.1763421
GAGTACT302500.025.91324820
TCAACGC348050.025.77371412
CAACGCA353800.025.34252413
Produced by FastQC (version 0.11.5)