FastQCFastQC Report
Thu 26 May 2016
SRR522090_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR522090_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33483537
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAG1575620.4705655797354981Illumina Paired End PCR Primer 2 (97% over 36bp)
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT396710.11847912005234094No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGA251550.034.36012625
TGCCGAG253100.034.13466626
AGATCGG234150.033.25826631
AATGCCG262200.033.0889624
CGAGATC252150.030.8571729
CCGAGAT255600.030.58647328
GAGATCG259050.030.18020630
GCCGAGA289400.029.98514427
GTTCAGC344700.025.62605914
GAATGCC352750.024.9314523
GGAATGC362900.024.42135422
TATGCCG30900.024.00401743
CGTATGC31450.023.72359741
TCGTATG31150.023.52754640
CAGGAAT383500.023.30622120
GACCGAT32100.022.82715432
CTCGTAT32750.022.44393339
AGGAATG405000.022.14125821
ACCGATC34450.021.78109433
GCAGGAA423750.021.34013219
Produced by FastQC (version 0.11.5)